BrsuB.01563.a

Dihydrodipicolinate synthase (EC 4.2.1.52)

CENTER ID: BrsuB.01563.a
ORGANISM: Brucella suis ATCC 23445
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuB.01563.a.B1.GE37993 Full length( BrsuB.01563.a ) 1 238
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruSui83806_0423
BV-BRC: fig|470137.4.peg.423
RefSeq: YP_001622232.1
UniProt: A9WY94

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MANGRVPVIA GVIDPATDRV IAHAKAAKDV GADAVVVTAP FYTITSQPEI IDHFRMVRDA VDIPLIAYDI PVCVHAKLTR QTVVTLANEG TIIGLKDSSG DDGNFRYALL DLAKNKNMFL MTGSEIVVDN ALLMGAHGVV PGLANVDPAG YVRLWDAAQR GDWVAARKEQ ERLCRLFEIV WVGAGRVSGG AAGVGAFKTA MRSLGIISTN KMARPRQALN TAEAGRIDAI LRSVGLLD
NT Sequence
GTGGCGAATG GCCGTGTTCC GGTCATTGCC GGTGTCATAG ACCCGGCGAC CGACCGCGTG ATTGCCCATG CAAAAGCCGC AAAGGACGTT GGCGCCGATG CGGTCGTCGT CACGGCTCCT TTCTATACAA TCACCAGCCA GCCCGAGATC ATCGATCATT TCCGCATGGT GCGCGATGCG GTCGATATTC CGCTGATTGC CTACGACATC CCGGTCTGCG TCCATGCCAA GCTTACACGC CAGACCGTGG TAACGCTTGC CAATGAAGGC ACGATCATCG GCCTGAAGGA TTCCTCCGGC GATGACGGCA ATTTCCGCTA TGCACTGCTC GATCTGGCTA AAAACAAAAA CATGTTCCTG ATGACCGGCT CGGAAATCGT GGTGGACAAT GCGCTTTTGA TGGGCGCGCA CGGCGTTGTT CCCGGCCTCG CCAATGTCGA TCCGGCAGGC TATGTGCGGC TTTGGGATGC CGCGCAGCGC GGCGACTGGG TGGCCGCGCG CAAGGAACAG GAGCGCCTTT GCCGCCTCTT CGAGATCGTC TGGGTTGGTG CGGGGCGCGT CAGCGGCGGA GCCGCAGGCG TCGGCGCTTT CAAGACCGCG ATGCGCAGTC TCGGCATTAT TTCCACCAAC AAGATGGCGC GCCCGCGCCA GGCGCTGAAT ACGGCGGAAG CAGGCCGCAT AGACGCGATC CTGCGCAGCG TCGGCCTCCT GGAT
Details for BrsuB.01563.a.B1.GE37993
HARVESTED ON: 5/12/2014
SEQUENCED ON: 5/4/2014
EXPECTED MW: 26kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV ANGRVPVIAG VIDPATDRVI AHAKAAKDVG ADAVVVTAPF YTITSQPEII DHFRMVRDAV DIPLIAYDIP VCVHAKLTRQ TVVTLANEGT IIGLKDSSGD DGNFRYALLD LAKNKNMFLM TGSEIVVDNA LLMGAHGVVP GLANVDPAGY VRLWDAAQRG DWVAARKEQE RLCRLFEIVW VGAGRVSGGA AGVGAFKTAM RSLGIISTNK MARPRQALNT AEAGRIDAIL RSVGLLD
Validated NT Sequence
atcttactca cttaatccag gaggccgacg ctgcgcagga tcgcgtctat gcggcctgct tccgccgtat tcagcgcctg gcgcgggcgc gccatcttgt tggtggaaat aatgccgaga ctgcgcatcg cggtcttgaa agcgccgacg cctgcggctc cgccgctgac gcgccccgca ccaacccaga cgatctcgaa gaggcggcaa aggcgctcct gttccttgcg cgcggccacc cagtcgccgc gctgcgcggc atcccaaagc cgcacatagc ctgccggatc gacattggcg aggccgggaa caacgccgtg cgcgcccatc aaaagcgcat tgtccaccac gatttccgag ccggtcatca ggaacatgtt tttgttttta gccagatcga gcagtgcata gcggaaattg ccgtcatcgc cggaggaatc cttcaggccg atgatcgtgc cttcattggc aagcgttacc acggtctggc gtgtaagctt ggcatggacg cagaccggga tgtcgtaggc aatcagcgga atatcgaccg catcgcgcac catgcggaaa tgatcgatga tctcgggctg gctggtgatt gtatagaaag gagccgtgac gacgaccgca tcggcgccaa cgtcctttgc ggcttttgca tgggcaatca cgcggtcggt cgccgggtct atgacaccgg caatgaccgg aacacggcca ttcgccacca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMA NGRVPVIAGV IDPATDRVIA HAKAAKDVGA DAVVVTAPFY TITSQPEIID HFRMVRDAVD IPLIAYDIPV CVHAKLTRQT VVTLANEGTI IGLKDSSGDD GNFRYALLDL AKNKNMFLMT GSEIVVDNAL LMGAHGVVPG LANVDPAGYV RLWDAAQRGD WVAARKEQER LCRLFEIVWV GAGRVSGGAA GVGAFKTAMR SLGIISTNKM ARPRQALNTA EAGRIDAILR SVGLLD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtggcga atggccgtgt tccggtcatt gccggtgtca tagacccggc gaccgaccgc gtgattgccc atgcaaaagc cgcaaaggac gttggcgccg atgcggtcgt cgtcacggct cctttctata caatcaccag ccagcccgag atcatcgatc atttccgcat ggtgcgcgat gcggtcgata ttccgctgat tgcctacgac atcccggtct gcgtccatgc caagcttaca cgccagaccg tggtaacgct tgccaatgaa ggcacgatca tcggcctgaa ggattcctcc ggcgatgacg gcaatttccg ctatgcactg ctcgatctgg ctaaaaacaa aaacatgttc ctgatgaccg gctcggaaat cgtggtggac aatgcgcttt tgatgggcgc gcacggcgtt gttcccggcc tcgccaatgt cgatccggca ggctatgtgc ggctttggga tgccgcgcag cgcggcgact gggtggccgc gcgcaaggaa caggagcgcc tttgccgcct cttcgagatc gtctgggttg gtgcggggcg cgtcagcggc ggagccgcag gcgtcggcgc tttcaagacc gcgatgcgca gtctcggcat tatttccacc aacaagatgg cgcgcccgcg ccaggcgctg aatacggcgg aagcaggccg catagacgcg atcctgcgca gcgtcggcct cctggattga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at