BrsuB.01370.a

Phosphoglucosamine mutase (EC 5.4.2.10)

CENTER ID: BrsuB.01370.a
ORGANISM: Brucella suis ATCC 23445
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuB.01370.a.B1.GE37992 Full length( BrsuB.01370.a ) 1 432
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruSui83806_1236
BV-BRC: fig|470137.4.peg.1236
RefSeq: YP_001622925.1
UniProt: A9WWG7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTPEIAMKVG MAVGYIFRRK GQASRVVIGK DTRRSGYMLE NALVAGFTAA GMDVFLLGPI PTPAVAMLCR SLRADIGVMI SASHNPFYDN GIKLFGPDGF KLSDQIELQI EAMIEGDMTP FLASHGDVGR AKRVDGDIYR YIEFAKRTLP RNISLNGLRV VVDCANGAGY KVAPAALWEL GAEVITINNE PNGININEDC GSTHPIGLMK KVHEVRADVG IALDGDADRV LLVDENGTVI DGDQLMAVIA ESWAASNRLE GGGIVATVMS NLGLERFLAD RNLTLARTKV GDRYVVEHMR EHGFNVGGEQ SGHIVLSDFA TTGDGLISAL QILAVAQEQN KPISDVCRKF QPVPQLLKNV RTTGGKPLEN KRVKSAIDEA KERLGGQGRL VIRPSGTEPL IRVMAEGDDR GLVEKVVNDI IDVISSESSA AA
NT Sequence
ATGACACCGG AAATCGCCAT GAAAGTGGGT ATGGCCGTTG GCTATATCTT CCGTCGCAAA GGTCAGGCCT CTCGCGTGGT GATCGGCAAG GATACACGCC GCTCCGGCTA TATGCTGGAA AATGCGCTGG TGGCCGGTTT TACCGCTGCC GGCATGGACG TGTTCCTGCT GGGCCCGATC CCGACCCCTG CCGTCGCCAT GCTCTGCCGC TCGCTGCGCG CTGATATCGG GGTGATGATT TCCGCGTCGC ATAATCCCTT CTACGATAAC GGCATCAAGC TTTTCGGCCC CGATGGCTTC AAGCTTTCGG ACCAGATCGA GCTTCAGATC GAGGCGATGA TCGAAGGCGA TATGACGCCT TTTCTTGCGA GCCATGGCGA TGTTGGCCGC GCCAAGCGCG TGGACGGCGA TATTTATCGC TATATCGAAT TTGCCAAGCG CACATTGCCG CGCAACATCT CGCTCAATGG GCTGCGCGTT GTGGTCGATT GCGCGAATGG CGCGGGTTAC AAGGTTGCGC CGGCTGCGCT GTGGGAACTG GGCGCGGAAG TCATTACCAT CAACAACGAG CCGAATGGCA TCAATATCAA CGAGGATTGC GGCTCGACGC ATCCCATCGG CCTTATGAAG AAAGTGCATG AGGTGCGCGC CGATGTGGGT ATCGCGCTCG ATGGCGATGC GGATCGTGTG CTTCTGGTCG ATGAAAACGG CACCGTCATC GATGGCGACC AGCTCATGGC TGTCATCGCC GAAAGCTGGG CTGCGAGCAA TCGCCTGGAA GGCGGCGGCA TTGTCGCCAC TGTCATGTCC AATCTCGGCC TGGAGCGTTT CTTGGCCGAC CGTAATCTGA CGCTTGCCCG TACCAAGGTT GGCGACCGCT ATGTGGTCGA GCACATGCGC GAGCATGGTT TCAATGTGGG TGGCGAGCAG TCGGGCCATA TCGTTCTGTC GGATTTTGCG ACGACTGGCG ACGGTCTTAT CTCCGCGCTC CAGATACTTG CCGTGGCGCA GGAACAGAAC AAGCCGATCA GCGATGTATG CCGCAAATTC CAGCCGGTGC CGCAATTGTT GAAGAATGTC CGCACCACAG GCGGCAAACC ACTTGAAAAC AAACGCGTGA AATCAGCGAT CGATGAGGCC AAGGAACGCC TTGGCGGTCA GGGGCGACTG GTTATCCGCC CGTCGGGCAC GGAGCCGCTC ATCCGCGTCA TGGCGGAAGG TGACGACCGT GGCCTGGTCG AAAAAGTGGT GAACGACATC ATTGATGTAA TCTCTTCGGA AAGCAGTGCT GCGGCC
Details for BrsuB.01370.a.B1.GE37992
HARVESTED ON: 5/12/2014
SEQUENCED ON: 5/5/2014
EXPECTED MW: 47kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MKVGMAVGYI FRRKGQASRV VIGKDTRRSG YMLENALVAG FTAAGMDVFL LGPIPTPAVA MLCRSLRADI GVMISASHNP FYDNGIKLFG PDGFKLSDQI ELQIEAMIEG DMTPFLASHG DVGRAKRVDG DIYRYIEFAK RTLPRNISLN GLRVVVDCAN GAGYKVAPAA LWELGAEVIT INNEPNGINI NEDCGSTHPI GLMKKVHEVR ADVGIALDGD ADRVLLVDEN GTVIDGDQLM AVIAESWAAS NRLEGGGIVA TVMSNLGLER FLADRNLTLA RTKVGDRYVV EHMREHGFNV GGEQSGHIVL SDFATTGDGL ISALQILAVA QEQNKPISDV CRKFQPVPQL LKNVRTTGGK PLENKRVKSA IDEAKERLGG QGRLVIRPSG TEPLIRVMAE GDDRGLVEKV VNDIIDV
Validated NT Sequence
gacaccggaa atcgccatga aagtgggtat ggccgttggc tatatcttcc gtcgcaaagg tcaggcctct cgcgtggtga tcggcaagga tacacgccgc tccggctata tgctggaaaa tgcgctggtg gccggtttta ccgctgccgg catggacgtg ttcctgctgg gcccgatccc gacccctgcc gtcgccatgc tctgccgctc gctgcgcgct gatatcgggg tgatgatttc cgcgtcgcat aatcccttct acgataacgg catcaagctt ttcggccccg atggcttcaa gctttcggac cagatcgagc ttcagatcga ggcgatgatc gaaggcgata tgacgccttt tcttgcgagc catggcgatg ttggccgcgc caagcgcgtg gacggcgata tttatcgcta tatcgaattt gccaagcgca cattgccgcg caacatctcg ctcaatgggc tgcgcgttgt ggtcgattgc gcgaatggcg cgggttacaa ggttgcgccg gctgcgctgt gggaactggg cgcggaagtc attaccatca acaacgagcc gaatggcatc aatatcaacg aggattgcgg ctcgacgcat cccatcggcc ttatgaagaa agtgcatgag gtgcgcgccg atgtgggtat cgcgctcgat ggcgatgcgg atcgtgtgct tctggtcgat gaaaacggca ccgtcatcga tggcgaccag ctcatggctg tcatcgccga aagctgggct gcgagcaatc gcctggaagg cggcggcatt gtcgccactg tcatgtccaa tctcggcctg gagcgtttct tggccgaccg taatctgacg cttgcccgta ccaaggttgg cgaccgctat gtggtcgagc acatgcgcga gcatggtttc aatgtgggtg gcgagcagtc gggccatatc gttctgtcgg attttgcgac gactggcgac ggtcttatct ccgcgctcca gatacttgcc gtggcgcagg aacagaacaa gccgatcagc gatgtatgcc gcaaattcca gccggtgccg caattgttga agaatgtccg caccacaggc ggcaaaccac ttgaaaacaa acgcgtgaaa tcagcgatcg atgaggccaa ggaacgcctt ggcggtcagg ggcgactggt tatccgcccg tcgggcacgg agccgctcat ccgcgtcatg gcggaaggtg acgaccgtgg cctggtcgaa aaagtggtga acgacatcat tgatgtaatc
Expected Protein Sequence
MAHHHHHHMT PEIAMKVGMA VGYIFRRKGQ ASRVVIGKDT RRSGYMLENA LVAGFTAAGM DVFLLGPIPT PAVAMLCRSL RADIGVMISA SHNPFYDNGI KLFGPDGFKL SDQIELQIEA MIEGDMTPFL ASHGDVGRAK RVDGDIYRYI EFAKRTLPRN ISLNGLRVVV DCANGAGYKV APAALWELGA EVITINNEPN GININEDCGS THPIGLMKKV HEVRADVGIA LDGDADRVLL VDENGTVIDG DQLMAVIAES WAASNRLEGG GIVATVMSNL GLERFLADRN LTLARTKVGD RYVVEHMREH GFNVGGEQSG HIVLSDFATT GDGLISALQI LAVAQEQNKP ISDVCRKFQP VPQLLKNVRT TGGKPLENKR VKSAIDEAKE RLGGQGRLVI RPSGTEPLIR VMAEGDDRGL VEKVVNDIID VISSESSAAA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacac cggaaatcgc catgaaagtg ggtatggccg ttggctatat cttccgtcgc aaaggtcagg cctctcgcgt ggtgatcggc aaggatacac gccgctccgg ctatatgctg gaaaatgcgc tggtggccgg ttttaccgct gccggcatgg acgtgttcct gctgggcccg atcccgaccc ctgccgtcgc catgctctgc cgctcgctgc gcgctgatat cggggtgatg atttccgcgt cgcataatcc cttctacgat aacggcatca agcttttcgg ccccgatggc ttcaagcttt cggaccagat cgagcttcag atcgaggcga tgatcgaagg cgatatgacg ccttttcttg cgagccatgg cgatgttggc cgcgccaagc gcgtggacgg cgatatttat cgctatatcg aatttgccaa gcgcacattg ccgcgcaaca tctcgctcaa tgggctgcgc gttgtggtcg attgcgcgaa tggcgcgggt tacaaggttg cgccggctgc gctgtgggaa ctgggcgcgg aagtcattac catcaacaac gagccgaatg gcatcaatat caacgaggat tgcggctcga cgcatcccat cggccttatg aagaaagtgc atgaggtgcg cgccgatgtg ggtatcgcgc tcgatggcga tgcggatcgt gtgcttctgg tcgatgaaaa cggcaccgtc atcgatggcg accagctcat ggctgtcatc gccgaaagct gggctgcgag caatcgcctg gaaggcggcg gcattgtcgc cactgtcatg tccaatctcg gcctggagcg tttcttggcc gaccgtaatc tgacgcttgc ccgtaccaag gttggcgacc gctatgtggt cgagcacatg cgcgagcatg gtttcaatgt gggtggcgag cagtcgggcc atatcgttct gtcggatttt gcgacgactg gcgacggtct tatctccgcg ctccagatac ttgccgtggc gcaggaacag aacaagccga tcagcgatgt atgccgcaaa ttccagccgg tgccgcaatt gttgaagaat gtccgcacca caggcggcaa accacttgaa aacaaacgcg tgaaatcagc gatcgatgag gccaaggaac gccttggcgg tcaggggcga ctggttatcc gcccgtcggg cacggagccg ctcatccgcg tcatggcgga aggtgacgac cgtggcctgg tcgaaaaagt ggtgaacgac atcattgatg taatctcttc ggaaagcagt gctgcggcct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat