SSGCID: Target Page

BrsuB.00674.a

Aspartate aminotransferase (EC 2.6.1.1)

CENTER ID:	BrsuB.00674.a
ORGANISM:	Brucella suis ATCC 23445
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuB.00674.a.B1.GE36979	Full length( BrsuB.00674.a )		1	342

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruSui83806_2529
BV-BRC:	fig\|470137.4.peg.2529
RefSeq:	YP_001627585.1
UniProt:	B0CLN5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVVRQAAERA LKDGRIGYTD ALGLIELREA IAAHYADHYG VSVSPERIAV TTGSSAAFNL AFIVYFDPGD RVAITRPGYP AYRNILTALG LEVVEIPTGH GTSSSLTAQA LAAAHAEKPL KGVLFASPAN PTGAVIDKPE LTAIIETARN LGIRVISDEI YHRLSYEADD VTALEVADDV TIINSFSKYY CMTGWRIGWM VLPDNDIRAI ERLSQSLYIS APELSQRGAI EAFRATAELE RIKDRYRRNR LILLNHLPRM GLDLAAPMDG AFYAYCDVSR FTNDSMEFVR GMISEINIAA TPGRDFDPLD GHRTVRFSYA GKVEEMEEAV RRLDDWLPQQ KA

NT Sequence

GTGGTCGTTC GTCAGGCCGC AGAGCGCGCG TTGAAAGATG GCCGCATCGG CTATACCGAC GCTTTGGGCC TGATTGAACT GCGCGAGGCT ATCGCCGCGC ATTATGCGGA TCATTACGGC GTCAGCGTGT CGCCGGAGCG GATTGCGGTC ACGACCGGCT CTTCCGCCGC TTTCAATCTT GCATTTATTG TCTATTTCGA TCCCGGCGAC CGGGTGGCGA TAACGCGCCC CGGCTATCCG GCCTACCGCA ACATCTTGAC GGCGCTTGGT CTCGAAGTGG TGGAAATTCC CACCGGCCAT GGAACATCCT CGTCGCTGAC GGCGCAAGCA CTTGCCGCCG CCCATGCTGA AAAACCCTTG AAGGGCGTTC TCTTTGCAAG CCCCGCCAAC CCAACCGGAG CGGTTATCGA CAAACCGGAA CTGACGGCGA TCATCGAGAC GGCACGTAAC CTCGGAATCC GGGTCATATC GGATGAAATC TATCATCGCC TGTCCTATGA AGCCGACGAT GTAACCGCAC TTGAAGTTGC CGACGACGTC ACCATCATCA ATTCCTTCTC CAAATATTAC TGCATGACGG GCTGGCGCAT CGGCTGGATG GTTCTGCCGG ACAATGATAT CCGGGCGATT GAGCGCCTCA GCCAGAGCCT CTATATTTCC GCGCCCGAAC TGTCGCAGCG CGGCGCCATT GAGGCCTTCC GCGCCACTGC GGAACTGGAA CGGATCAAGG ATCGCTATCG CCGAAACAGG CTTATCCTGC TCAATCATCT GCCGCGCATG GGGCTTGATC TGGCTGCACC GATGGACGGC GCATTCTACG CCTATTGCGA TGTGTCGCGT TTCACCAATG ACAGCATGGA GTTTGTGCGC GGGATGATCT CCGAGATCAA TATCGCCGCC ACCCCCGGCC GTGATTTCGA TCCGCTGGAC GGGCATCGCA CCGTGCGTTT CTCCTACGCA GGCAAGGTCG AGGAAATGGA AGAGGCTGTT CGGCGGCTTG ACGACTGGTT GCCGCAGCAG AAAGCG

Details for BrsuB.00674.a.B1.GE36979

HARVESTED ON:	5/21/2013
SEQUENCED ON:	5/23/2013
EXPECTED MW:	39kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	89

Validated AA Sequence

MAHHHHHHMV VVRQAAERAL KDGRIGYTDA LGLIELREAI AAHYADHYGV SVSPERIAVT TGSSAAFNLA FIVYFDPGDR VAITRPGYPA YRNILTALGL EVVEIPTGHG TFSSLTAQAL AAAHAEKPLK GVLFASPANP TGAVIDKPEL TAIIETARNL GIRVISDEIY HRLSYEADDV TALEVADDVT IINSFSKYYC MTGWRIGWMV LPDNDIRAIE RLSQSLYISA PELSQRGAIE AFRATAELER IKDRYRRNRL ILLNHLPRMG LDLAAPMDGA FYAYCDVSRF TXDSMXFVRG XISEINIAAT PXP

Validated NT Sequence

tttgtttanc tttaagaagg agatatacca tggctcacca ccaccaccac catatggtgg tcgttcgtca ggccgcagag cgcgcgttga aagatggccg catcggctat accgacgctt tgggcctgat tgaactgcgc gaggctatcg ccgcgcatta tgcggatcat tacggcgtca gcgtgtcgcc ggagcggatt gcggtcacga ccggctcttc cgccgctttc aatcttgcat ttattgtcta tttcgatccc ggcgaccggg tggcgataac gcgccccggc tatccggcct accgcaacat cttgacggcg cttggtctcg aagtggtgga aattcccacc ggccatggaa cattctcgtc gctgacggcg caagcacttg ccgccgccca tgctgaaaaa cccttgaagg gcgttctctt tgcaagcccc gccaacccaa ccggagcggt tatcgacaaa ccggaactga cggcgatcat cgagacggca cgtaacctcg gaatccgggt catatcggat gaaatctatc atcgcctgtc ctatgaagcc gacgatgtaa ccgcacttga agttgccgac gacgtcacca tcatcaattc cttctccaaa tattactgca tgacgggctg gcgcatcggc tggatggttc tgccggacaa cgatatccgg gcgattgagc gcctcagcca gagcctctat atttccgcgc ccgaactgtc gcagcgcggc gccattgagg ccttccgcgc cactgcggaa ctggaacgga tcaaggatcg ctatcgccga aacaggctta tcctgctcaa tcatctgccg cgcatggggc ttgatctggc tgcaccgatg gacggcgcat tctacgccta ttgcgatgtg tcgcgtttca ccnatgacag catggnnttt gtgcgcgggn ngatctccga gatcaatatc gccgccaccc ccnggccgtg atttcgatcc gctggacggg catcgnnncg tgcgtttctc ctacncnggc aaggtcgagg aaatgnnnan nngntnnnnn gcttgacnac tggnngccgc annnnnaaan cgtaannnan taannnnnnn cnnnnnnnnn nnnnnnnnnn nnnnnnnna

Expected Protein Sequence

MAHHHHHHMV VRQAAERALK DGRIGYTDAL GLIELREAIA AHYADHYGVS VSPERIAVTT GSSAAFNLAF IVYFDPGDRV AITRPGYPAY RNILTALGLE VVEIPTGHGT SSSLTAQALA AAHAEKPLKG VLFASPANPT GAVIDKPELT AIIETARNLG IRVISDEIYH RLSYEADDVT ALEVADDVTI INSFSKYYCM TGWRIGWMVL PDNDIRAIER LSQSLYISAP ELSQRGAIEA FRATAELERI KDRYRRNRLI LLNHLPRMGL DLAAPMDGAF YAYCDVSRFT NDSMEFVRGM ISEINIAATP GRDFDPLDGH RTVRFSYAGK VEEMEEAVRR LDDWLPQQKA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtggtcg ttcgtcaggc cgcagagcgc gcgttgaaag atggccgcat cggctatacc gacgctttgg gcctgattga actgcgcgag gctatcgccg cgcattatgc ggatcattac ggcgtcagcg tgtcgccgga gcggattgcg gtcacgaccg gctcttccgc cgctttcaat cttgcattta ttgtctattt cgatcccggc gaccgggtgg cgataacgcg ccccggctat ccggcctacc gcaacatctt gacggcgctt ggtctcgaag tggtggaaat tcccaccggc catggaacat cctcgtcgct gacggcgcaa gcacttgccg ccgcccatgc tgaaaaaccc ttgaagggcg ttctctttgc aagccccgcc aacccaaccg gagcggttat cgacaaaccg gaactgacgg cgatcatcga gacggcacgt aacctcggaa tccgggtcat atcggatgaa atctatcatc gcctgtccta tgaagccgac gatgtaaccg cacttgaagt tgccgacgac gtcaccatca tcaattcctt ctccaaatat tactgcatga cgggctggcg catcggctgg atggttctgc cggacaatga tatccgggcg attgagcgcc tcagccagag cctctatatt tccgcgcccg aactgtcgca gcgcggcgcc attgaggcct tccgcgccac tgcggaactg gaacggatca aggatcgcta tcgccgaaac aggcttatcc tgctcaatca tctgccgcgc atggggcttg atctggctgc accgatggac ggcgcattct acgcctattg cgatgtgtcg cgtttcacca atgacagcat ggagtttgtg cgcgggatga tctccgagat caatatcgcc gccacccccg gccgtgattt cgatccgctg gacgggcatc gcaccgtgcg tttctcctac gcaggcaagg tcgaggaaat ggaagaggct gttcggcggc ttgacgactg gttgccgcag cagaaagcgt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat