BrsuB.00629.a

Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases

CENTER ID: BrsuB.00629.a
ORGANISM: Brucella suis ATCC 23445
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuB.00629.a.B1.GE37121 Full length( BrsuB.00629.a ) 1 504
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruSui83806_0478
BV-BRC: fig|470137.4.peg.478
RefSeq: YP_001622271.1
UniProt: A9WYD3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MALRAISIDN TLTELTFEDL RDMSARVGNM LADAGISAGD VVAGLLPRTP ELVATILGAW RIGAIYQPLF TAFGPKAIEQ RFGTSGAKLV VTNLANRSKL AEVENCPRVA TILAPGESLP EGDIDFRAAV AAASTECEPV MRKGSDLFMM MSTSGTAGLP KGVPVPLRAL MAFGAYMRDA VGLRSDDIFW NIADPGWAYG LYYAVTGPLL LGVPTILNEG GFTAENTYDI IERLGVTSLA GSPTAFRLLM AAGPESAARV KGRLRVVSSA GEPLNPEVIR WFDACLGAPI HDHYAQTELG MVVNNHHGLE HPVRQGSAGY AMPGYRVAVL DEAGKEVDPN EPGVLAIDID NSPLLWFTGY YKKDTPSISG GYYRTGDTVE FEPDGSISFI GRADDVITSS GYRIGPFDVE SALLEHPAVN EAAVVGVPDP QRTEIVKAFV ILAPGFEGTP ELAEELALHV KKQLSAHTYP RQIDFVAELP KTPSGKIQRF LLRKAEVEKQ QQQN
NT Sequence
GTGGCGCTGC GCGCCATTTC CATCGACAAT ACGTTGACTG AACTCACCTT TGAAGACCTG CGCGATATGT CCGCGCGGGT CGGCAATATG CTGGCCGATG CCGGGATCAG CGCAGGCGAT GTGGTTGCCG GTCTTCTCCC CCGCACGCCT GAACTCGTCG CCACCATTCT CGGCGCCTGG CGCATCGGCG CAATCTATCA GCCGCTTTTC ACCGCCTTCG GCCCAAAAGC CATCGAACAG CGTTTCGGCA CCAGTGGCGC CAAACTCGTC GTCACCAACC TGGCCAATCG TTCCAAATTG GCCGAAGTGG AAAACTGCCC CCGCGTCGCA ACCATTCTTG CCCCCGGTGA AAGCCTGCCG GAAGGCGATA TCGACTTTCG CGCAGCCGTG GCGGCAGCAT CGACCGAATG CGAGCCCGTC ATGCGCAAGG GCAGCGACCT TTTCATGATG ATGTCCACCT CCGGCACGGC GGGCCTGCCC AAAGGCGTCC CGGTGCCGCT GCGCGCCCTG ATGGCTTTCG GCGCCTATAT GCGCGATGCC GTGGGCCTGC GCAGCGACGA TATTTTCTGG AATATCGCCG ATCCGGGCTG GGCATATGGG CTTTACTATG CCGTGACGGG TCCCCTGCTT CTGGGTGTGC CGACGATCCT GAACGAAGGC GGTTTCACCG CCGAAAACAC CTATGACATC ATCGAACGCC TCGGCGTGAC GAGCCTTGCG GGCTCTCCGA CTGCCTTCCG CCTGCTTATG GCCGCCGGGC CGGAATCTGC TGCCCGCGTC AAGGGCCGCT TGCGCGTGGT ATCCAGCGCG GGCGAGCCGC TGAACCCGGA AGTCATTCGC TGGTTCGATG CCTGTCTTGG CGCGCCGATC CACGATCATT ACGCCCAGAC GGAACTGGGC ATGGTGGTGA ACAACCATCA TGGATTGGAA CATCCGGTGC GGCAGGGTTC GGCGGGCTAT GCCATGCCGG GCTACAGGGT TGCGGTTCTG GATGAAGCGG GCAAAGAAGT CGACCCCAAT GAACCGGGCG TTCTGGCCAT TGATATCGAC AATTCGCCGC TTCTCTGGTT CACCGGCTAT TATAAAAAGG ATACGCCCTC CATCTCCGGC GGATATTATC GCACCGGCGA TACGGTGGAA TTCGAGCCGG ATGGGTCTAT CAGCTTCATC GGCCGCGCCG ATGACGTCAT CACCTCCTCC GGCTATCGCA TCGGGCCTTT CGATGTGGAA AGCGCGCTTC TGGAACATCC TGCGGTCAAC GAGGCTGCGG TGGTCGGCGT TCCCGATCCG CAACGCACCG AAATCGTGAA GGCCTTTGTC ATCCTGGCCC CCGGCTTCGA GGGTACGCCG GAACTGGCGG AAGAACTGGC GCTTCACGTC AAGAAACAGC TTTCCGCCCA TACCTATCCG CGGCAGATCG ACTTTGTGGC GGAGCTTCCC AAGACGCCGA GCGGAAAAAT ACAGCGCTTC CTCCTGCGCA AGGCGGAAGT CGAAAAGCAA CAGCAACAGA AT
Details for BrsuB.00629.a.B1.GE37121
HARVESTED ON: 7/17/2013
SEQUENCED ON: 7/20/2013
EXPECTED MW: 55kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 90
PERCENT COVERAGE: 67
Validated AA Sequence
MAHHHHHHMV ALRAISIDNT LTELTFEDLR DMSARVGNML ADAGISAGDV VAGLLPRTPE LVATILGAWR IGAIYQPLFT AFGPKAIEQR FGTSGAKLVV TNLANRSKLA EVENCPRVAT ILAPGESLPE GDIDFRAAVA AASTECEPVM RKGSDLFMMM STSGTAGLPK GVPVPLRALM AFGAYMRDAV GLRSDDIFWN IADPGWAYGL YYAVTGPLLL GVPTILNEGG FTAENTYDII ERLGVTSLAG SPTAFRLLMA AGPESAARVK GRLRVVSSAG EPLNPEVIRW FDACLGAPIX DHXXQXXLGM XVNXXHGXXI XXXXVRXXMX XXXRXXXXEX XXXXX
Validated NT Sequence
ttntnnnntt taagaaggag anataccatg gctcaccacc accaccacca tatggtggcg ctgcgcgcca tttccatcga caatacgttg actgaactca cctttgaaga cctgcgcgat atgtccgcgc gggtcggcaa tatgctggcc gatgccggga tcagcgcagg cgatgtggtt gccggtcttc tcccccgcac gcctgaactc gtcgccacca ttctcggcgc ctggcgcatc ggcgcaatct atcagccgct tttcaccgcc ttcggcccaa aagccatcga acagcgtttc ggcaccagtg gcgccaaact cgtcgtcacc aacctggcca atcgttccaa attggccgaa gtggaaaact gcccccgcgt cgcaaccatt cttgcccccg gtgaaagcct gccggaaggc gatatcgact ttcgcgcagc cgtggcggca gcatcgaccg aatgcgagcc cgtcatgcgc aagggcagcg accttttcat gatgatgtcc acctccggca cggcgggcct gcccaaaggc gtcccggtgc cgctgcgcgc cctgatggct ttcggcgcct atatgcgcga tgccgtgggc ctgcgcagcg acgatatttt ctggaatatc gccgatccgg gctgggcata tgggctttac tatgccgtga cgggtcccct gcttctgggt gtgccgacga tcctgaacga aggcggtttc accgccgaaa acacctatga catcatcgaa cgcctcggcg tgacgagcct tgcgggctct ccgactgcct tccgcctgct tatggccgcc gggccggaat ctgctgcccg cgtcaagggc cgcttgcgcg tggtatccag cgcgggcgag ccgctgaacc cggaagtcat tcgctggttc gatgcctgtc ttggcgcgcc gatccncgat cattannncc agangganct gggnatggng gtgaacancn ntcatggatt nnncatcnnn ngcnncnggg ttcggcnggn tatgncatgn nnnntacggn ngnnnntggn ngagcnngna anantngncc nntgannngg nntnnntnnn nncgancnnn nnnncngnnn nnnnnncnnn nnnnnnnnnn nnnntnnnnn nnnnnnnnnn nnnnnnnnnn nntnnnnnnn nnnnnnnnnn ntnt
Expected Protein Sequence
MAHHHHHHMA LRAISIDNTL TELTFEDLRD MSARVGNMLA DAGISAGDVV AGLLPRTPEL VATILGAWRI GAIYQPLFTA FGPKAIEQRF GTSGAKLVVT NLANRSKLAE VENCPRVATI LAPGESLPEG DIDFRAAVAA ASTECEPVMR KGSDLFMMMS TSGTAGLPKG VPVPLRALMA FGAYMRDAVG LRSDDIFWNI ADPGWAYGLY YAVTGPLLLG VPTILNEGGF TAENTYDIIE RLGVTSLAGS PTAFRLLMAA GPESAARVKG RLRVVSSAGE PLNPEVIRWF DACLGAPIHD HYAQTELGMV VNNHHGLEHP VRQGSAGYAM PGYRVAVLDE AGKEVDPNEP GVLAIDIDNS PLLWFTGYYK KDTPSISGGY YRTGDTVEFE PDGSISFIGR ADDVITSSGY RIGPFDVESA LLEHPAVNEA AVVGVPDPQR TEIVKAFVIL APGFEGTPEL AEELALHVKK QLSAHTYPRQ IDFVAELPKT PSGKIQRFLL RKAEVEKQQQ QN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtggcgc tgcgcgccat ttccatcgac aatacgttga ctgaactcac ctttgaagac ctgcgcgata tgtccgcgcg ggtcggcaat atgctggccg atgccgggat cagcgcaggc gatgtggttg ccggtcttct cccccgcacg cctgaactcg tcgccaccat tctcggcgcc tggcgcatcg gcgcaatcta tcagccgctt ttcaccgcct tcggcccaaa agccatcgaa cagcgtttcg gcaccagtgg cgccaaactc gtcgtcacca acctggccaa tcgttccaaa ttggccgaag tggaaaactg cccccgcgtc gcaaccattc ttgcccccgg tgaaagcctg ccggaaggcg atatcgactt tcgcgcagcc gtggcggcag catcgaccga atgcgagccc gtcatgcgca agggcagcga ccttttcatg atgatgtcca cctccggcac ggcgggcctg cccaaaggcg tcccggtgcc gctgcgcgcc ctgatggctt tcggcgccta tatgcgcgat gccgtgggcc tgcgcagcga cgatattttc tggaatatcg ccgatccggg ctgggcatat gggctttact atgccgtgac gggtcccctg cttctgggtg tgccgacgat cctgaacgaa ggcggtttca ccgccgaaaa cacctatgac atcatcgaac gcctcggcgt gacgagcctt gcgggctctc cgactgcctt ccgcctgctt atggccgccg ggccggaatc tgctgcccgc gtcaagggcc gcttgcgcgt ggtatccagc gcgggcgagc cgctgaaccc ggaagtcatt cgctggttcg atgcctgtct tggcgcgccg atccacgatc attacgccca gacggaactg ggcatggtgg tgaacaacca tcatggattg gaacatccgg tgcggcaggg ttcggcgggc tatgccatgc cgggctacag ggttgcggtt ctggatgaag cgggcaaaga agtcgacccc aatgaaccgg gcgttctggc cattgatatc gacaattcgc cgcttctctg gttcaccggc tattataaaa aggatacgcc ctccatctcc ggcggatatt atcgcaccgg cgatacggtg gaattcgagc cggatgggtc tatcagcttc atcggccgcg ccgatgacgt catcacctcc tccggctatc gcatcgggcc tttcgatgtg gaaagcgcgc ttctggaaca tcctgcggtc aacgaggctg cggtggtcgg cgttcccgat ccgcaacgca ccgaaatcgt gaaggccttt gtcatcctgg cccccggctt cgagggtacg ccggaactgg cggaagaact ggcgcttcac gtcaagaaac agctttccgc ccatacctat ccgcggcaga tcgactttgt ggcggagctt cccaagacgc cgagcggaaa aatacagcgc ttcctcctgc gcaaggcgga agtcgaaaag caacagcaac agaattgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat