SSGCID: Target Page

BrsuB.00323.a

ZINC PROTEASE (EC 3.4.99.-)

CENTER ID:	BrsuB.00323.a
ORGANISM:	Brucella suis ATCC 23445
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuB.00323.a.B1.GE37987	Full length( BrsuB.00323.a )		1	335

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruSui83806_0213

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSFSASQDSV SGGVRMLAEN RDAVTDLVAL AVNEPRFDQE AIDRIRQQIV AGIEAAQRNP STIASRKFAE VLYGNHPYAR DDEGTVKSLQ SISRDDLANF HRKNFARDKL TVGVVGAINA KDLGVMLDRI FGDLPASAEL VPVPDAKLAL GTTTSLNFDM PQTSISFVYP AIPRKDPEFF AAYLMNHILG GGFTSRLYNE VREKRGLAYS VSSSMVMHDH VSELMISTAT RPDKAQDSLK IIREQVAAMA NDGPTEEELA AAKSFLKGSY AVNNLDSSGA IANTLVSLQE AGLPSDYIDK RSELIDAVTL DQVKAIARKL LQAEPAILIY GPAQS

NT Sequence

ATGAGTTTTT CCGCTTCGCA GGATTCGGTT TCCGGCGGCG TCCGTATGCT GGCCGAAAAT CGTGATGCCG TGACCGATCT CGTGGCGCTT GCCGTCAACG AGCCGCGCTT CGATCAGGAA GCCATCGACC GCATTCGCCA GCAGATTGTT GCAGGCATTG AGGCCGCACA GCGCAATCCG TCTACGATTG CGTCGCGCAA GTTTGCCGAA GTGCTTTACG GCAACCACCC TTATGCGCGT GACGATGAGG GAACCGTGAA ATCCCTGCAA TCGATCAGCC GCGATGATCT TGCGAATTTC CATCGCAAGA ATTTCGCCCG CGACAAGCTG ACCGTTGGCG TTGTGGGTGC CATCAACGCC AAGGATCTGG GCGTCATGCT CGACAGGATT TTTGGCGACC TGCCCGCCTC GGCGGAACTG GTTCCGGTGC CGGATGCGAA GCTTGCGCTT GGCACCACCA CCAGCCTGAA TTTCGATATG CCGCAGACGT CGATCAGCTT CGTCTACCCG GCCATTCCGC GCAAAGACCC TGAGTTTTTT GCCGCCTATC TGATGAACCA TATTCTGGGC GGCGGTTTCA CCTCGCGTCT TTATAACGAA GTGCGTGAAA AGCGCGGCCT TGCCTATTCG GTGTCCTCGT CCATGGTCAT GCACGACCAT GTTTCCGAAC TGATGATTTC CACCGCCACC CGGCCTGACA AGGCCCAGGA CTCATTGAAA ATCATTCGTG AGCAGGTTGC CGCCATGGCC AATGACGGCC CGACCGAGGA AGAGCTTGCC GCAGCCAAGA GCTTTCTCAA AGGCTCCTAT GCGGTGAACA ATCTCGATTC GTCGGGAGCT ATTGCCAATA CGCTGGTGAG TTTGCAGGAA GCCGGGCTTC CAAGCGATTA TATCGACAAG CGTTCCGAAC TCATCGATGC TGTCACGCTG GATCAGGTCA AGGCCATTGC CAGGAAACTG CTTCAGGCCG AGCCTGCGAT CCTGATCTAT GGACCGGCGC AAAGT

Details for BrsuB.00323.a.B1.GE37987

HARVESTED ON:	5/12/2014
SEQUENCED ON:	5/4/2014
EXPECTED MW:	37kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	100
PERCENT COVERAGE:	61

Validated AA Sequence

MLDRIFGDLP ASAELVPVPD AKLALGTTTS LNFDMPQTSI SFVYPAIPRK DPEFFAAYLM NHILGGGFTS RLYNEVREKR GLAYSVSSSM VMHDHVSELM ISTATRPDKA QDSLKIIREQ VAAMANDGPT EEELAAAKSF LKGSYAVNNL DSSGAIANTL VSLQEAGLPS DYIDKRSELI DAVTLDQVKA IARKLLQAEP AILIYGPAQS

Validated NT Sequence

ttccgcttcg caggattcgg tttccggcgg cgtccgtatg ctggccgaaa atcgtgatgc cgtgaccgat ctcgtggcgc ttgccgtcaa cgagccgcgc ttcgatcagg aagccatcga ccgcattcgc cagcagattg ttgcaggcat tgaggccgca cagcgcaatc cgtctacgat tgcgtcgcgc aagtttgccg aagtgcttta cggcaaccac ccttatgcgc gtgacgatga gggaaccgtg aaatccctgc aatcgatcag ccgcgatgat cttgcgaatt tccatcgcaa gaatttcgcc cgcgacaagc tgaccgttgg cgttgtgggt gccatcaacg ccnaaggatc tgggcgtcat gctcgacagg atttttggcg acctgcccgc ctcggcggaa ctggttccgg tgccggatgc gaagcttgcg cttggcacca ccaccagcct gaatttcgat atgccgcaga cgtcgatcag cttcgtctac ccggccattc cgcgcaaaga ccctgagttt tttgccgcct atctgatgaa ccatattctg ggcggcggtt tcacctcgcg tctttataac gaagtgcgtg aaaagcgcgg ccttgcctat tcggtgtcct cgtccatggt catgcacgac catgtttccg aactgatgat ttccaccgcc acccggcctg acaaggccca ggactcattg aaaatcattc gtgagcaggt tgccgccatg gccaatgacg gcccgaccga ggaagagctt gccgcagcca agagctttct caaaggctcc tatgcggtga acaatctcga ttcgtcggga gctattgcca atacgctggt gagtttgcag gaagccgggc ttccaagcga ttatatcgac aagcgttccg aactcatcga tgctgtcacg ctggatcagg tcaaggccat tgccaggaaa ctgcttcagg ccgagcctgc gatcctgatc tatggaccgg cgcaaagtta agtgagtaag atagganccg g

Expected Protein Sequence

MAHHHHHHMS FSASQDSVSG GVRMLAENRD AVTDLVALAV NEPRFDQEAI DRIRQQIVAG IEAAQRNPST IASRKFAEVL YGNHPYARDD EGTVKSLQSI SRDDLANFHR KNFARDKLTV GVVGAINAKD LGVMLDRIFG DLPASAELVP VPDAKLALGT TTSLNFDMPQ TSISFVYPAI PRKDPEFFAA YLMNHILGGG FTSRLYNEVR EKRGLAYSVS SSMVMHDHVS ELMISTATRP DKAQDSLKII REQVAAMAND GPTEEELAAA KSFLKGSYAV NNLDSSGAIA NTLVSLQEAG LPSDYIDKRS ELIDAVTLDQ VKAIARKLLQ AEPAILIYGP AQS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagtt tttccgcttc gcaggattcg gtttccggcg gcgtccgtat gctggccgaa aatcgtgatg ccgtgaccga tctcgtggcg cttgccgtca acgagccgcg cttcgatcag gaagccatcg accgcattcg ccagcagatt gttgcaggca ttgaggccgc acagcgcaat ccgtctacga ttgcgtcgcg caagtttgcc gaagtgcttt acggcaacca cccttatgcg cgtgacgatg agggaaccgt gaaatccctg caatcgatca gccgcgatga tcttgcgaat ttccatcgca agaatttcgc ccgcgacaag ctgaccgttg gcgttgtggg tgccatcaac gccaaggatc tgggcgtcat gctcgacagg atttttggcg acctgcccgc ctcggcggaa ctggttccgg tgccggatgc gaagcttgcg cttggcacca ccaccagcct gaatttcgat atgccgcaga cgtcgatcag cttcgtctac ccggccattc cgcgcaaaga ccctgagttt tttgccgcct atctgatgaa ccatattctg ggcggcggtt tcacctcgcg tctttataac gaagtgcgtg aaaagcgcgg ccttgcctat tcggtgtcct cgtccatggt catgcacgac catgtttccg aactgatgat ttccaccgcc acccggcctg acaaggccca ggactcattg aaaatcattc gtgagcaggt tgccgccatg gccaatgacg gcccgaccga ggaagagctt gccgcagcca agagctttct caaaggctcc tatgcggtga acaatctcga ttcgtcggga gctattgcca atacgctggt gagtttgcag gaagccgggc ttccaagcga ttatatcgac aagcgttccg aactcatcga tgctgtcacg ctggatcagg tcaaggccat tgccaggaaa ctgcttcagg ccgagcctgc gatcctgatc tatggaccgg cgcaaagttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat