BrsuB.00143.e
oxidoreductase, short-chain dehydrogenase/reductase family
| CENTER ID: | BrsuB.00143.e |
| ORGANISM: | Brucella suis ATCC 23445 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrsuB.00143.e.B1.GE37119 | Full length( BrsuB.00143.e ) | 1 | 257 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrsuB.00143.e.B1.PS01915 | Full length( BrsuB.00143.e ) | 1 | 257 | ||
| BrsuB.00143.e.B1.PS01916 | Full length( BrsuB.00143.e ) | 1 | 257 |
Structures
4NPC
DEPOSITED: 11/21/2013
DETERMINATION: XRay
CLONE: BrsuB.00143.e.B1.GE37119
PROTEIN: BrsuB.00143.e.B1.PS01915
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | PATRIC_Locus_Tag:VBIBruSui83806_3279 |
| BV-BRC: | fig|470137.4.peg.3279 |
| RefSeq: | YP_001628283.1 |
| UniProt: | B0CIA7 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDQAPQIDL NFPLSEKVAI VTGGASGIGA AISKAFIAKG AKVAVLDISA DIAKAKAEEL GENAKPFVCD VSSQQSVNDA ITAVISQFGK IDIAVNSAGV VYLAPAEDIS LDYWDKTINI NLKGSFLVTQ AVGRAMIAAG NGGKIINLAS QAGTVAIEEH VAYCASKFGV IGMSKTFAAE WGKYGICVNT LSPTIVLTEL GKKAWAGEKG EAAKKRIPAG RFAYPEEIAA AAVFLASAGA DMITGADLLI DGGYTIL
NT Sequence
ATGTCCGATC AAGCTCCCCA AATCGACCTG AACTTCCCGC TCTCTGAAAA AGTCGCCATC GTGACCGGAG GCGCTTCCGG TATCGGTGCT GCAATTTCCA AGGCTTTCAT CGCTAAAGGA GCCAAGGTCG CTGTGCTCGA CATCAGCGCA GACATTGCGA AAGCCAAAGC CGAGGAGCTT GGCGAAAACG CAAAGCCGTT CGTTTGCGAT GTTTCCAGCC AGCAATCGGT GAATGATGCA ATCACGGCTG TCATAAGCCA GTTCGGCAAG ATCGATATTG CGGTCAATAG TGCCGGTGTC GTCTATCTGG CGCCAGCGGA AGATATTTCG CTCGATTACT GGGACAAGAC GATCAACATT AATCTGAAGG GCAGCTTTCT CGTCACCCAG GCTGTCGGCC GCGCAATGAT CGCCGCAGGC AATGGCGGCA AGATAATCAA TCTCGCCTCA CAGGCCGGAA CCGTCGCCAT TGAAGAACAC GTCGCCTATT GCGCGTCGAA GTTCGGTGTC ATCGGCATGT CGAAGACATT CGCAGCCGAA TGGGGCAAGT ATGGCATCTG CGTCAACACG CTGTCCCCGA CCATCGTGCT CACCGAACTT GGCAAGAAGG CCTGGGCCGG TGAAAAGGGT GAGGCCGCAA AGAAACGCAT TCCGGCGGGC CGCTTCGCCT ATCCCGAAGA GATTGCAGCC GCGGCCGTGT TCCTTGCTTC TGCTGGAGCC GACATGATTA CGGGTGCCGA TCTTCTGATC GATGGCGGTT ACACCATTCT T
Details for BrsuB.00143.e.B1.GE37119
| HARVESTED ON: | 7/17/2013 |
| SEQUENCED ON: | 7/20/2013 |
| EXPECTED MW: | 27kDa |
| OBSERVED MW: | 27kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMS DQAPQIDLNF PLSEKVAIVT GGASGIGAAI SKAFIAKGAK VAVLDISADI AKAKAEELGE NAKPFVCDVS SQQSVNDAIT AVISQFGKID IAVNSAGVVY LAPAEDISLD YWDKTININL KGSFLVTQAV GRAMIAAGNG GKIINLASQA GTVAIEEHVA YCASKFGVIG MSKTFAAEWG KYGICVNTLS PTIVLTELGK KAWAGEKGEA AKKRIPAGRF AYPEEIAAAA VFLASAGADM ITGADLLIDG GYTIL
Validated NT Sequence
tcttactcac ttaaagaatg gtgtaaccgc catcgatcag aagatcggca cccgtaatca tgtcggctcc agcagaagca aggaacacgg ccgcggctgc aatctcttcg ggataggcga agcggcccgc cggaatgcgt ttctttgcgg cctcaccctt ttcaccggcc caggccttct tgccaagttc ggtgagcaca atggtcgggg acagcgtgtt gacgcagatg ccatacttgc cccattcggc tgcgaatgtc ttcgacatgc cgatgacacc gaacttcgac gcgcaatagg cgacgtgttc ttcaatggcg acggttccgg cctgtgaggc gagattgatt atcttgccgc cattgcctgc ggcgatcatt gcgcggccga cagcctgggt gacgagaaag ctgcccttca gattaatgtt gatcgtcttg tcccagtaat cgagcgaaat atcttccgct ggcgccagat agacgacacc ggcactattg accgcaatat cgatcttgcc gaactggctt atgacagccg tgattgcatc attcaccgat tgctggctgg aaacatcgca aacgaacggc tttgcgtttt cgccaagctc ctcggctttg gctttcgcaa tgtctgcgct gatgtcgagc acagcgacct tggctccttt agcgatgaaa gccttggaaa ttgcagcacc gataccggaa gcgcctccgg tcacgatggc gactttttca gagagcggga agttcaggtc gatttgggga gcttgatcgg acatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMS DQAPQIDLNF PLSEKVAIVT GGASGIGAAI SKAFIAKGAK VAVLDISADI AKAKAEELGE NAKPFVCDVS SQQSVNDAIT AVISQFGKID IAVNSAGVVY LAPAEDISLD YWDKTININL KGSFLVTQAV GRAMIAAGNG GKIINLASQA GTVAIEEHVA YCASKFGVIG MSKTFAAEWG KYGICVNTLS PTIVLTELGK KAWAGEKGEA AKKRIPAGRF AYPEEIAAAA VFLASAGADM ITGADLLIDG GYTIL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtccg atcaagctcc ccaaatcgac ctgaacttcc cgctctctga aaaagtcgcc atcgtgaccg gaggcgcttc cggtatcggt gctgcaattt ccaaggcttt catcgctaaa ggagccaagg tcgctgtgct cgacatcagc gcagacattg cgaaagccaa agccgaggag cttggcgaaa acgcaaagcc gttcgtttgc gatgtttcca gccagcaatc ggtgaatgat gcaatcacgg ctgtcataag ccagttcggc aagatcgata ttgcggtcaa tagtgccggt gtcgtctatc tggcgccagc ggaagatatt tcgctcgatt actgggacaa gacgatcaac attaatctga agggcagctt tctcgtcacc caggctgtcg gccgcgcaat gatcgccgca ggcaatggcg gcaagataat caatctcgcc tcacaggccg gaaccgtcgc cattgaagaa cacgtcgcct attgcgcgtc gaagttcggt gtcatcggca tgtcgaagac attcgcagcc gaatggggca agtatggcat ctgcgtcaac acgctgtccc cgaccatcgt gctcaccgaa cttggcaaga aggcctgggc cggtgaaaag ggtgaggccg caaagaaacg cattccggcg ggccgcttcg cctatcccga agagattgca gccgcggccg tgttccttgc ttctgctgga gccgacatga ttacgggtgc cgatcttctg atcgatggcg gttacaccat tctttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for BrsuB.00143.e.B1.PS01915
| PURIFICATION DATe: | 10/1/2013 |
| CONCENTRATION: | 40.2mg/ml |
| OBSERVED MW: | 27kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS DQAPQIDLNF PLSEKVAIVT GGASGIGAAI SKAFIAKGAK VAVLDISADI AKAKAEELGE NAKPFVCDVS SQQSVNDAIT AVISQFGKID IAVNSAGVVY LAPAEDISLD YWDKTININL KGSFLVTQAV GRAMIAAGNG GKIINLASQA GTVAIEEHVA YCASKFGVIG MSKTFAAEWG KYGICVNTLS PTIVLTELGK KAWAGEKGEA AKKRIPAGRF AYPEEIAAAA VFLASAGADM ITGADLLIDG GYTIL
Validated NT Sequence
tcttactcac ttaaagaatg gtgtaaccgc catcgatcag aagatcggca cccgtaatca tgtcggctcc agcagaagca aggaacacgg ccgcggctgc aatctcttcg ggataggcga agcggcccgc cggaatgcgt ttctttgcgg cctcaccctt ttcaccggcc caggccttct tgccaagttc ggtgagcaca atggtcgggg acagcgtgtt gacgcagatg ccatacttgc cccattcggc tgcgaatgtc ttcgacatgc cgatgacacc gaacttcgac gcgcaatagg cgacgtgttc ttcaatggcg acggttccgg cctgtgaggc gagattgatt atcttgccgc cattgcctgc ggcgatcatt gcgcggccga cagcctgggt gacgagaaag ctgcccttca gattaatgtt gatcgtcttg tcccagtaat cgagcgaaat atcttccgct ggcgccagat agacgacacc ggcactattg accgcaatat cgatcttgcc gaactggctt atgacagccg tgattgcatc attcaccgat tgctggctgg aaacatcgca aacgaacggc tttgcgtttt cgccaagctc ctcggctttg gctttcgcaa tgtctgcgct gatgtcgagc acagcgacct tggctccttt agcgatgaaa gccttggaaa ttgcagcacc gataccggaa gcgcctccgg tcacgatggc gactttttca gagagcggga agttcaggtc gatttgggga gcttgatcgg acatatggtg gtggtggtgg tgagccat
Expressed Protein Sequence
MAHHHHHHMS DQAPQIDLNF PLSEKVAIVT GGASGIGAAI SKAFIAKGAK VAVLDISADI AKAKAEELGE NAKPFVCDVS SQQSVNDAIT AVISQFGKID IAVNSAGVVY LAPAEDISLD YWDKTININL KGSFLVTQAV GRAMIAAGNG GKIINLASQA GTVAIEEHVA YCASKFGVIG MSKTFAAEWG KYGICVNTLS PTIVLTELGK KAWAGEKGEA AKKRIPAGRF AYPEEIAAAA VFLASAGADM ITGADLLIDG GYTIL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCCG ATCAAGCTCC CCAAATCGAC CTGAACTTCC CGCTCTCTGA AAAAGTCGCC ATCGTGACCG GAGGCGCTTC CGGTATCGGT GCTGCAATTT CCAAGGCTTT CATCGCTAAA GGAGCCAAGG TCGCTGTGCT CGACATCAGC GCAGACATTG CGAAAGCCAA AGCCGAGGAG CTTGGCGAAA ACGCAAAGCC GTTCGTTTGC GATGTTTCCA GCCAGCAATC GGTGAATGAT GCAATCACGG CTGTCATAAG CCAGTTCGGC AAGATCGATA TTGCGGTCAA TAGTGCCGGT GTCGTCTATC TGGCGCCAGC GGAAGATATT TCGCTCGATT ACTGGGACAA GACGATCAAC ATTAATCTGA AGGGCAGCTT TCTCGTCACC CAGGCTGTCG GCCGCGCAAT GATCGCCGCA GGCAATGGCG GCAAGATAAT CAATCTCGCC TCACAGGCCG GAACCGTCGC CATTGAAGAA CACGTCGCCT ATTGCGCGTC GAAGTTCGGT GTCATCGGCA TGTCGAAGAC ATTCGCAGCC GAATGGGGCA AGTATGGCAT CTGCGTCAAC ACGCTGTCCC CGACCATCGT GCTCACCGAA CTTGGCAAGA AGGCCTGGGC CGGTGAAAAG GGTGAGGCCG CAAAGAAACG CATTCCGGCG GGCCGCTTCG CCTATCCCGA AGAGATTGCA GCCGCGGCCG TGTTCCTTGC TTCTGCTGGA GCCGACATGA TTACGGGTGC CGATCTTCTG ATCGATGGCG GTTACACCAT TCTTTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT
Details for BrsuB.00143.e.B1.PS01916
| PURIFICATION DATe: | 10/1/2013 |
| CONCENTRATION: | 52.7mg/ml |
| OBSERVED MW: | 27kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS DQAPQIDLNF PLSEKVAIVT GGASGIGAAI SKAFIAKGAK VAVLDISADI AKAKAEELGE NAKPFVCDVS SQQSVNDAIT AVISQFGKID IAVNSAGVVY LAPAEDISLD YWDKTININL KGSFLVTQAV GRAMIAAGNG GKIINLASQA GTVAIEEHVA YCASKFGVIG MSKTFAAEWG KYGICVNTLS PTIVLTELGK KAWAGEKGEA AKKRIPAGRF AYPEEIAAAA VFLASAGADM ITGADLLIDG GYTIL
Validated NT Sequence
tcttactcac ttaaagaatg gtgtaaccgc catcgatcag aagatcggca cccgtaatca tgtcggctcc agcagaagca aggaacacgg ccgcggctgc aatctcttcg ggataggcga agcggcccgc cggaatgcgt ttctttgcgg cctcaccctt ttcaccggcc caggccttct tgccaagttc ggtgagcaca atggtcgggg acagcgtgtt gacgcagatg ccatacttgc cccattcggc tgcgaatgtc ttcgacatgc cgatgacacc gaacttcgac gcgcaatagg cgacgtgttc ttcaatggcg acggttccgg cctgtgaggc gagattgatt atcttgccgc cattgcctgc ggcgatcatt gcgcggccga cagcctgggt gacgagaaag ctgcccttca gattaatgtt gatcgtcttg tcccagtaat cgagcgaaat atcttccgct ggcgccagat agacgacacc ggcactattg accgcaatat cgatcttgcc gaactggctt atgacagccg tgattgcatc attcaccgat tgctggctgg aaacatcgca aacgaacggc tttgcgtttt cgccaagctc ctcggctttg gctttcgcaa tgtctgcgct gatgtcgagc acagcgacct tggctccttt agcgatgaaa gccttggaaa ttgcagcacc gataccggaa gcgcctccgg tcacgatggc gactttttca gagagcggga agttcaggtc gatttgggga gcttgatcgg acatatggtg gtggtggtgg tgagccat
Expressed Protein Sequence
MAHHHHHHMS DQAPQIDLNF PLSEKVAIVT GGASGIGAAI SKAFIAKGAK VAVLDISADI AKAKAEELGE NAKPFVCDVS SQQSVNDAIT AVISQFGKID IAVNSAGVVY LAPAEDISLD YWDKTININL KGSFLVTQAV GRAMIAAGNG GKIINLASQA GTVAIEEHVA YCASKFGVIG MSKTFAAEWG KYGICVNTLS PTIVLTELGK KAWAGEKGEA AKKRIPAGRF AYPEEIAAAA VFLASAGADM ITGADLLIDG GYTIL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCCG ATCAAGCTCC CCAAATCGAC CTGAACTTCC CGCTCTCTGA AAAAGTCGCC ATCGTGACCG GAGGCGCTTC CGGTATCGGT GCTGCAATTT CCAAGGCTTT CATCGCTAAA GGAGCCAAGG TCGCTGTGCT CGACATCAGC GCAGACATTG CGAAAGCCAA AGCCGAGGAG CTTGGCGAAA ACGCAAAGCC GTTCGTTTGC GATGTTTCCA GCCAGCAATC GGTGAATGAT GCAATCACGG CTGTCATAAG CCAGTTCGGC AAGATCGATA TTGCGGTCAA TAGTGCCGGT GTCGTCTATC TGGCGCCAGC GGAAGATATT TCGCTCGATT ACTGGGACAA GACGATCAAC ATTAATCTGA AGGGCAGCTT TCTCGTCACC CAGGCTGTCG GCCGCGCAAT GATCGCCGCA GGCAATGGCG GCAAGATAAT CAATCTCGCC TCACAGGCCG GAACCGTCGC CATTGAAGAA CACGTCGCCT ATTGCGCGTC GAAGTTCGGT GTCATCGGCA TGTCGAAGAC ATTCGCAGCC GAATGGGGCA AGTATGGCAT CTGCGTCAAC ACGCTGTCCC CGACCATCGT GCTCACCGAA CTTGGCAAGA AGGCCTGGGC CGGTGAAAAG GGTGAGGCCG CAAAGAAACG CATTCCGGCG GGCCGCTTCG CCTATCCCGA AGAGATTGCA GCCGCGGCCG TGTTCCTTGC TTCTGCTGGA GCCGACATGA TTACGGGTGC CGATCTTCTG ATCGATGGCG GTTACACCAT TCTTTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT