BrsuB.00143.d
Short chain dehydrogenase
| CENTER ID: | BrsuB.00143.d |
| ORGANISM: | Brucella suis ATCC 23445 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrsuB.00143.d.B1.GE37118 | Full length( BrsuB.00143.d ) | 1 | 246 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrsuB.00143.d.B1.PS01901 | Full length( BrsuB.00143.d ) | 1 | 246 |
Structures
4NI5
DEPOSITED: 11/5/2013
DETERMINATION: XRay
CLONE: BrsuB.00143.d.B1.GE37118
PROTEIN: BrsuB.00143.d.B1.PS01901
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | PATRIC_Locus_Tag:VBIBruSui83806_2753 |
| BV-BRC: | fig|470137.4.peg.2753 |
| RefSeq: | YP_001627793.1 |
| UniProt: | B0CGS2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRFKDKVVIV TGGASGIGEA TARAFIREGA KVVIADFSDH GQQLADERAG AHEQALFIKT DVADTRAVQA LIARTVENYG RLDIMFANAG IAADAPIDEL DEAAWQKTID INLTGVYLCD KYAIDQMRSQ GGGVIVNCGS IHSHVGKSGV TAYAAAKGGV KLLTQTLAID YGPQNIRVNA VCPGYIDTPL LKNIPDDKKQ ALVALHPMGR LGRAEEVANA VLFLASDEAS FVNGASLLVD GGYTAQ
NT Sequence
ATGCGGTTCA AGGACAAGGT GGTCATCGTA ACAGGTGGAG CAAGCGGCAT CGGGGAAGCC ACGGCGCGGG CTTTTATCCG GGAGGGCGCA AAGGTCGTGA TTGCGGATTT TTCCGATCAT GGCCAGCAAT TGGCGGATGA ACGGGCTGGC GCCCATGAAC AGGCGCTATT CATCAAGACG GATGTTGCCG ATACCAGGGC CGTGCAGGCG CTGATTGCAA GGACGGTGGA AAACTATGGG CGGCTCGACA TCATGTTCGC AAATGCAGGC ATTGCAGCCG ATGCCCCCAT CGACGAACTG GACGAAGCCG CATGGCAGAA AACCATCGAT ATAAACCTGA CGGGGGTTTA TCTCTGCGAT AAATACGCCA TCGACCAGAT GCGCTCGCAA GGCGGTGGGG TGATCGTCAA TTGCGGCTCC ATCCATAGCC ATGTCGGCAA GTCGGGCGTT ACAGCTTATG CCGCCGCCAA GGGCGGTGTG AAACTTCTCA CGCAAACACT TGCCATTGAT TACGGCCCCC AGAACATTCG CGTGAACGCG GTCTGCCCCG GCTATATCGA TACGCCGCTT CTCAAGAATA TCCCCGACGA CAAAAAACAG GCCCTCGTGG CGCTTCATCC CATGGGGCGT CTTGGCAGGG CCGAGGAGGT TGCAAACGCC GTCCTGTTCC TCGCAAGCGA CGAAGCTTCC TTCGTCAACG GTGCATCGCT TCTGGTCGAC GGCGGCTATA CCGCCCAA
Details for BrsuB.00143.d.B1.GE37118
| HARVESTED ON: | 7/17/2013 |
| SEQUENCED ON: | 7/20/2013 |
| EXPECTED MW: | 27kDa |
| OBSERVED MW: | 27kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with sequence variation |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMR FKDKVVIVTG GASGIGEATA RAFIREGAKV VIADFSDHGQ QLADERAGAH EQALFIKTDV ADTRAVQALI ARTVENYGRL DIMFANAGIA ADAPIDELDE AAWQKTIDIN LTGVYLCDKY AIDQMRSQGG GVIVNCGSIH SHVGKSGVTA YAAAKGGVKL LTQTLAIDYG PQNIRVNAVC PGYIDTPLLK NIPDDKKQAL VALHPMGRLG RAEEVANAVL FLASDEASFV NGASLLVDGG YTAQ
Validated NT Sequence
tcttactcac ttattgggcg gtatagccgc cgtcgaccag aagcgatgca ccgttgacga aggaagcttc gtcgcttgcg aggaacagga cggcgtttgc aacctcctcg gccctgccaa gacgccccat gggatgaagc gccacgaggg cctgtttttt gtcgtcgggg atattcttga gaagcggcgt atcgatatag ccggggcaga ccgcgttcac gcgaatgttc tgggggccgt aatcaatggc aagtgtttgc gtgagaagtt tcacaccgcc cttggcggcg gcataagctg taacgcccga cttgccgaca tggctatgga tggagccgca attgacgatc accccaccgc cttgcgagcg catctggtcg atggcgtatt tatcgcagag ataaaccccc gtcaggttta tatcgatggt tttctgccat gcggcttcgt ccagttcgtc gatgggggca tcggctgcaa tgcctgcatt tgcaaacatg atgtcgagcc gcccatagtt ttccaccgtc cttgcaatca gcgcctgcac ggccctggta tcggcaacat ccgtcttgat gaatagcgcc tgttcatggg cgccagcccg ttcatccgcc aattgctggc catgatcgga aaaatccgca atcacgacct ttgcgccctc ccggataaaa gcccgcgccg tggcttcccc gatgccgctt gctccacctg ttacgatgac caccttgtcc ttgaaccgca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMR FKDKVVIVTG GASGIGEATA RAFIREGAKV VIADFSDHGQ QLADERAGAH EQALFIKTDV ADTRAVQALI ARTVENYGRL DIMFANAGIA ADAPIDELDE AAWQKTIDIN LTGVYLCDKY AIDQMRSQGG GVIVNCGSIH SHVGKSGVTA YAAAKGGVKL LTQTLAIDYG PQNIRVNAVC PGYIDTPLLK NIPDDKKQAL VALHPMGRLG RAEEVANAVL FLASDEASFV NGASLLVDGG YTAQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcggt tcaaggacaa ggtggtcatc gtaacaggtg gagcaagcgg catcggggaa gccacggcgc gggcttttat ccgggagggc gcaaaggtcg tgattgcgga tttttccgat catggccagc aattggcgga tgaacgggct ggcgcccatg aacaggcgct attcatcaag acggatgttg ccgataccag ggccgtgcag gcgctgattg caaggacggt ggaaaactat gggcggctcg acatcatgtt cgcaaatgca ggcattgcag ccgatgcccc catcgacgaa ctggacgaag ccgcatggca gaaaaccatc gatataaacc tgacgggggt ttatctctgc gataaatacg ccatcgacca gatgcgctcg caaggcggtg gggtgatcgt caattgcggc tccatccata gccatgtcgg caagtcgggc gttacagctt atgccgccgc caagggcggt gtgaaacttc tcacgcaaac acttgccatt gattacggcc cccagaacat tcgcgtgaac gcggtctgcc ccggctatat cgatacgccg cttctcaaga atatccccga cgacaaaaaa caggccctcg tggcgcttca tcccatgggg cgtcttggca gggccgagga ggttgcaaac gccgtcctgt tcctcgcaag cgacgaagct tccttcgtca acggtgcatc gcttctggtc gacggcggct ataccgccca atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for BrsuB.00143.d.B1.PS01901
| PURIFICATION DATe: | 9/19/2013 |
| CONCENTRATION: | 61.2mg/ml |
| OBSERVED MW: | 28kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMR FKDKVVIVTG GASGIGEATA RAFIREGAKV VIADFSDHGQ QLADERAGAH EQALFIKTDV ADTRAVQALI ARTVENYGRL DIMFANAGIA ADAPIDELDE AAWQKTIDIN LTGVYLCDKY AIDQMRSQGG GVIVNCGSIH SHVGKSGVTA YAAAKGGVKL LTQTLAIDYG PQNIRVNAVC PGYIDTPLLK NIPDDKKQAL VALHPMGRLG RAEEVANAVL FLASDEASFV NGASLLVDGG YTAQ
Validated NT Sequence
tcttactcac ttattgggcg gtatagccgc cgtcgaccag aagcgatgca ccgttgacga aggaagcttc gtcgcttgcg aggaacagga cggcgtttgc aacctcctcg gccctgccaa gacgccccat gggatgaagc gccacgaggg cctgtttttt gtcgtcgggg atattcttga gaagcggcgt atcgatatag ccggggcaga ccgcgttcac gcgaatgttc tgggggccgt aatcaatggc aagtgtttgc gtgagaagtt tcacaccgcc cttggcggcg gcataagctg taacgcccga cttgccgaca tggctatgga tggagccgca attgacgatc accccaccgc cttgcgagcg catctggtcg atggcgtatt tatcgcagag ataaaccccc gtcaggttta tatcgatggt tttctgccat gcggcttcgt ccagttcgtc gatgggggca tcggctgcaa tgcctgcatt tgcaaacatg atgtcgagcc gcccatagtt ttccaccgtc cttgcaatca gcgcctgcac ggccctggta tcggcaacat ccgtcttgat gaatagcgcc tgttcatggg cgccagcccg ttcatccgcc aattgctggc catgatcgga aaaatccgca atcacgacct ttgcgccctc ccggataaaa gcccgcgccg tggcttcccc gatgccgctt gctccacctg ttacgatgac caccttgtcc ttgaaccgca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHMR FKDKVVIVTG GASGIGEATA RAFIREGAKV VIADFSDHGQ QLADERAGAH EQALFIKTDV ADTRAVQALI ARTVENYGRL DIMFANAGIA ADAPIDELDE AAWQKTIDIN LTGVYLCDKY AIDQMRSQGG GVIVNCGSIH SHVGKSGVTA YAAAKGGVKL LTQTLAIDYG PQNIRVNAVC PGYIDTPLLK NIPDDKKQAL VALHPMGRLG RAEEVANAVL FLASDEASFV NGASLLVDGG YTAQ
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCGGT TCAAGGACAA GGTGGTCATC GTAACAGGTG GAGCAAGCGG CATCGGGGAA GCCACGGCGC GGGCTTTTAT CCGGGAGGGC GCAAAGGTCG TGATTGCGGA TTTTTCCGAT CATGGCCAGC AATTGGCGGA TGAACGGGCT GGCGCCCATG AACAGGCGCT ATTCATCAAG ACGGATGTTG CCGATACCAG GGCCGTGCAG GCGCTGATTG CAAGGACGGT GGAAAACTAT GGGCGGCTCG ACATCATGTT CGCAAATGCA GGCATTGCAG CCGATGCCCC CATCGACGAA CTGGACGAAG CCGCATGGCA GAAAACCATC GATATAAACC TGACGGGGGT TTATCTCTGC GATAAATACG CCATCGACCA GATGCGCTCG CAAGGCGGTG GGGTGATCGT CAATTGCGGC TCCATCCATA GCCATGTCGG CAAGTCGGGC GTTACAGCTT ATGCCGCCGC CAAGGGCGGT GTGAAACTTC TCACGCAAAC ACTTGCCATT GATTACGGCC CCCAGAACAT TCGCGTGAAC GCGGTCTGCC CCGGCTATAT CGATACGCCG CTTCTCAAGA ATATCCCCGA CGACAAAAAA CAGGCCCTCG TGGCGCTTCA TCCCATGGGG CGTCTTGGCA GGGCCGAGGA GGTTGCAAAC GCCGTCCTGT TCCTCGCAAG CGACGAAGCT TCCTTCGTCA ACGGTGCATC GCTTCTGGTC GACGGCGGCT ATACCGCCCA ATGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT