BrsuB.00111.b

glutamine synthetase family protein

CENTER ID: BrsuB.00111.b
ORGANISM: Brucella suis ATCC 23445
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuB.00111.b.B1.GE37985 Full length( BrsuB.00111.b ) 1 396
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruSui83806_0794
BV-BRC: fig|470137.4.peg.794
RefSeq: YP_001622552.1
UniProt: A9WZ64

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTISGAYPE DGYGFHYPED DGDLKLLPDL TTLSAVPWET DSTAQVICDL VYQDGRAVEF TPRNVLRNVI AAYSKRGLKP VVAPEIEFYL VRKNPDPDYP LTPPVGRSGR AIGGGQGYSI AGVNEFDELI DDIYHFSEGQ GLEIDTLIHE EGAGQLEINL RHGDPVELAD QVFMFKRTIR EAALKHDMYA TFMAKPIQGQ PGSAMHIHQS IVDKKTGRNI FTNEDGSESQ AFHHFLGGMQ RHVPNALVMF APYVNSYRRL TPDASAPVNV KWGYDNRTTA FRVPRSDPSA RRVENRIPSS DANPYLALAA SLACGLIGLV NKIEPEQPVT TSVNTKEVEL PRGLIDAVEL FEEDAELRNL FGSSFMTTYA AIKRAEFETF MEVISPWERE FLLLNV
NT Sequence
ATGACGACGA TTTCCGGCGC TTACCCCGAG GATGGCTATG GTTTCCATTA TCCCGAAGAC GATGGCGACC TGAAGCTTCT GCCCGATCTT ACCACCCTCT CTGCCGTGCC ATGGGAAACG GATTCGACGG CGCAGGTGAT CTGCGATCTC GTCTATCAGG ACGGGCGCGC GGTGGAATTC ACCCCGCGCA ATGTGCTGCG CAATGTCATT GCCGCCTATA GCAAGCGCGG CCTGAAGCCG GTTGTCGCCC CGGAAATCGA GTTTTATCTG GTGCGCAAAA ACCCGGATCC GGACTATCCG CTGACGCCGC CTGTTGGCCG TTCGGGCCGG GCCATTGGTG GCGGACAGGG CTATTCCATT GCCGGTGTCA ACGAGTTCGA TGAACTGATC GACGATATCT ATCATTTCTC GGAAGGTCAG GGGCTGGAGA TCGACACGCT GATCCATGAA GAAGGTGCTG GCCAGCTTGA AATAAACCTG CGCCATGGCG ACCCGGTGGA ACTGGCCGAT CAGGTTTTCA TGTTCAAGCG CACGATCCGC GAGGCGGCAC TGAAACACGA TATGTACGCC ACGTTCATGG CCAAGCCCAT TCAGGGCCAG CCGGGCTCGG CCATGCATAT CCACCAGTCC ATCGTGGACA AGAAGACCGG ACGAAACATC TTCACCAATG AAGACGGCAG CGAAAGCCAA GCCTTCCACC ATTTCCTTGG CGGGATGCAG CGCCATGTGC CCAATGCACT CGTCATGTTC GCCCCTTATG TGAACTCCTA CCGCCGCCTC ACGCCGGACG CCTCGGCCCC GGTCAACGTC AAATGGGGCT ATGACAACCG CACAACCGCC TTCCGCGTGC CGCGTTCAGA CCCCAGTGCG CGCCGCGTGG AAAACCGCAT CCCCTCTTCC GACGCCAATC CCTACCTTGC CCTTGCTGCT TCGCTTGCTT GCGGCCTGAT CGGCCTTGTA AACAAGATCG AGCCGGAACA GCCCGTGACC ACGAGCGTCA ACACCAAGGA GGTCGAGTTG CCCCGCGGCC TCATCGACGC GGTGGAGCTT TTCGAGGAAG ACGCCGAATT GCGCAATCTG TTCGGCAGTT CCTTCATGAC CACCTATGCC GCCATCAAAC GCGCCGAGTT CGAGACCTTC ATGGAAGTCA TCAGCCCCTG GGAACGGGAG TTTTTGTTGC TCAATGTT
Details for BrsuB.00111.b.B1.GE37985
HARVESTED ON: 5/12/2014
SEQUENCED ON: 5/4/2014
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 90
PERCENT COVERAGE: 69
Validated AA Sequence
MAHHHHHHMT TISGAYPEDG YGFHYPEDXG DLKLLPDLTT LSAVPWETDS TAQVICDLVY QDGRAVEFTP RNVLRNVIAA YSKRGLKPVV APEIEFYLVR KNPDPDYPLT PPVGRSGRAI GGGQGYSIAG VNEFDELIDD IYHFSEGQGL EIDTLIHEEG AGQLEINLRH GDPVELADQV FMFKRTIREA ALKHDMYATF MAKPIQGQPG SAMHIHQSIV DKKTGRNIFT NEDGSESQAF HHFLGGDAAP CAQCTRHVRP LCELLPPPHA GRLGPGQRQM GL
Validated NT Sequence
cnnnnnnnnt ctagatattt tgtttacttt aagaaggaga tataccatgg ctcaccacca ccaccaccat atgacgacga tttccggcgc ttaccccgag gatggctatg gtttccatta tcccgaagac ganggcgacc tgaagcttct gcccgatctt accaccctct ctgccgtgcc atgggaaacg gattcgacgg cgcaggtgat ctgcgatctc gtctatcagg acgggcgcgc ggtggaattc accccgcgca atgtgctgcg caatgtcatt gccgcctata gcaagcgcgg cctgaagccg gttgtcgccc cggaaatcga gttttatctg gtgcgcaaaa acccggatcc ggactatccg ctgacgccgc ctgttggccg ttcgggccgg gccattggtg gcggacaggg ctattccatt gccggtgtca acgagttcga tgaactgatc gacgatatct atcatttctc ggaaggtcag gggctggaga tcgacacgct gatccatgaa gaaggtgctg gccagcttga aataaacctg cgccatggcg acccggtgga actggccgat caggttttca tgttcaagcg cacgatccgc gaggcggcac tgaaacacga tatgtacgcc acgttcatgg ccaagcccat tcagggccag ccgggctcgg ccatgcatat ccaccagtcc atcgtggaca agaagaccgg acgaaacatc ttcaccaatg aagacggcag cgaaagccaa gccttccacc atttccttgg cggggatgca gcgccatgtg cccaatgcac tcgtcatgtt cgccccttat gtgaactcct accgccgcct cacgccggac gcctcggccc cggtcaacgt caaatggggc tatgacaacc gcacaaccgc cttccgcgtg ccgcgttcag accccagtgc gcgccgcgtg gaaaaccgca tcccctcttc cgacgccaat ccctaccttg cccttgctgc ttcgcttgct tgcggcctga tcggnttgta aacaagatcg agccngacag cccgcgacca cganngtcac accnaggagg tcgagttgnc ccnnngccnc atcgannnng gtgggagctt tnnaggaaga acgncnaant nnncgcaatc tgntcnnnan tnnncatgga nnnnctatnn nnntnnnnnn nnnnnntnga nnnnatgnag nnnnnccnnn nnnnnnntnn nnagtnnna
Expected Protein Sequence
MAHHHHHHMT TISGAYPEDG YGFHYPEDDG DLKLLPDLTT LSAVPWETDS TAQVICDLVY QDGRAVEFTP RNVLRNVIAA YSKRGLKPVV APEIEFYLVR KNPDPDYPLT PPVGRSGRAI GGGQGYSIAG VNEFDELIDD IYHFSEGQGL EIDTLIHEEG AGQLEINLRH GDPVELADQV FMFKRTIREA ALKHDMYATF MAKPIQGQPG SAMHIHQSIV DKKTGRNIFT NEDGSESQAF HHFLGGMQRH VPNALVMFAP YVNSYRRLTP DASAPVNVKW GYDNRTTAFR VPRSDPSARR VENRIPSSDA NPYLALAASL ACGLIGLVNK IEPEQPVTTS VNTKEVELPR GLIDAVELFE EDAELRNLFG SSFMTTYAAI KRAEFETFME VISPWEREFL LLNV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacga cgatttccgg cgcttacccc gaggatggct atggtttcca ttatcccgaa gacgatggcg acctgaagct tctgcccgat cttaccaccc tctctgccgt gccatgggaa acggattcga cggcgcaggt gatctgcgat ctcgtctatc aggacgggcg cgcggtggaa ttcaccccgc gcaatgtgct gcgcaatgtc attgccgcct atagcaagcg cggcctgaag ccggttgtcg ccccggaaat cgagttttat ctggtgcgca aaaacccgga tccggactat ccgctgacgc cgcctgttgg ccgttcgggc cgggccattg gtggcggaca gggctattcc attgccggtg tcaacgagtt cgatgaactg atcgacgata tctatcattt ctcggaaggt caggggctgg agatcgacac gctgatccat gaagaaggtg ctggccagct tgaaataaac ctgcgccatg gcgacccggt ggaactggcc gatcaggttt tcatgttcaa gcgcacgatc cgcgaggcgg cactgaaaca cgatatgtac gccacgttca tggccaagcc cattcagggc cagccgggct cggccatgca tatccaccag tccatcgtgg acaagaagac cggacgaaac atcttcacca atgaagacgg cagcgaaagc caagccttcc accatttcct tggcgggatg cagcgccatg tgcccaatgc actcgtcatg ttcgcccctt atgtgaactc ctaccgccgc ctcacgccgg acgcctcggc cccggtcaac gtcaaatggg gctatgacaa ccgcacaacc gccttccgcg tgccgcgttc agaccccagt gcgcgccgcg tggaaaaccg catcccctct tccgacgcca atccctacct tgcccttgct gcttcgcttg cttgcggcct gatcggcctt gtaaacaaga tcgagccgga acagcccgtg accacgagcg tcaacaccaa ggaggtcgag ttgccccgcg gcctcatcga cgcggtggag cttttcgagg aagacgccga attgcgcaat ctgttcggca gttccttcat gaccacctat gccgccatca aacgcgccga gttcgagacc ttcatggaag tcatcagccc ctgggaacgg gagtttttgt tgctcaatgt ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat