SSGCID: Target Page

BrsuB.00107.b

RND efflux system, membrane fusion protein CmeA

CENTER ID:	BrsuB.00107.b
ORGANISM:	Brucella suis ATCC 23445
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuB.00107.b.B1.GE37980	Full length( BrsuB.00107.b )		1	304

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruSui83806_0353
BV-BRC:	fig\|470137.4.peg.353
RefSeq:	YP_001622169.1
UniProt:	A9WY31

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLFALQKDAL QAALDGAQAN LAKAQADADN LKLQTERARS LYKQKTVSQA MLDDRVAAEK QALAVVQQAQ ASLEQAQINL GYTDIRAPFS GRIGMANFSV GALVGPSSGP LATIVSQDPI YVTFPVSDKT ILDLTEGGRT ATDRSNVAVS LTLSNGMTYP QTGAIDFTGI KINPNTDTLM VRAQFPNPNN VLIDGQYVQV TAASKYPVEA LLVPQKAIMT DQSGNYVLAV GEDNKVIQRQ ITQGSTFGSN VVVKSGLAVG DQVVVDGLQR IRPGQKVDPQ IVDATTPAQS HVGGELIHAV IRLH

NT Sequence

TTGCTGTTTG CCCTGCAAAA GGATGCGCTT CAGGCGGCGC TGGATGGGGC GCAGGCCAAT CTTGCAAAAG CGCAGGCCGA TGCCGACAAC CTGAAACTCC AGACCGAGCG CGCCCGCTCG CTCTACAAGC AGAAGACCGT TTCGCAAGCC ATGCTTGATG ATCGTGTGGC AGCGGAAAAG CAGGCTTTGG CCGTCGTGCA GCAGGCGCAG GCTTCGCTGG AACAGGCGCA AATCAATCTC GGCTATACCG ATATACGCGC GCCTTTTTCC GGACGCATCG GCATGGCCAA TTTCAGCGTC GGCGCCCTTG TCGGGCCGTC CTCAGGCCCT CTGGCAACGA TCGTCAGCCA GGACCCGATT TACGTCACCT TCCCCGTCAG CGACAAGACC ATTCTCGATC TAACCGAAGG CGGGAGAACG GCCACAGACC GCAGCAATGT AGCCGTCAGC CTGACGCTCT CCAACGGCAT GACATATCCG CAAACGGGGG CCATCGACTT TACCGGGATC AAGATTAATC CCAATACCGA TACGCTGATG GTCCGGGCAC AGTTTCCCAA TCCGAACAAT GTCCTGATCG ACGGGCAGTA TGTTCAGGTA ACGGCGGCGA GCAAATACCC CGTTGAAGCC CTGCTCGTCC CGCAAAAGGC AATCATGACC GACCAGAGCG GCAATTACGT TCTGGCCGTC GGTGAAGATA ACAAGGTCAT CCAGCGCCAG ATCACGCAAG GTTCGACCTT TGGCAGCAAT GTTGTGGTCA AGTCCGGCCT CGCGGTGGGC GATCAGGTCG TGGTCGATGG CCTCCAGCGC ATCCGGCCCG GCCAGAAGGT CGATCCGCAG ATCGTCGATG CAACCACGCC CGCCCAAAGC CATGTCGGTG GGGAATTGAT CCATGCTGTC ATCCGTCTTC AT

Details for BrsuB.00107.b.B1.GE37980

HARVESTED ON:	5/12/2014
SEQUENCED ON:	5/4/2014
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHML LFALQKDALQ AALDAAQANL AKAQADADNL KLQTERARSL YKQKTVSQAM LDDRVAAEKQ ALAVVQQAQA SLEQAQINLG YTDIRAPFSG RIGMANFSVG ALVGPSSGPL ATIVSQDPIY VTFPVSDKTI LDLTEGGRTA TDRSNVAVSL TLSNGMTYPQ TGAIDFTGIK INPNTDTLMV RAQFPNPNNV LIDGQYVQVT AASKHPVEAL LVPQKAIMTD QSGNYVLAVG EDNKVIQRQI TQGSTFGSNV VVKSGLAVGD QVVVDGLQRI RPGQKVDPQI VDATTPAQKA MSVGN

Validated NT Sequence

tactcactta atgaagacgg atgacagcat ggatcaattc cccaccgaca tggctttctg ggcgggcgtg gttgcatcga cgatctgcgg atcgaccttc tggccgggcc ggatgcgctg gaggccatcg accacgacct gatcgcccac cgcgaggccg gacttgacca caacattgct gccaaaggtc gaaccttgcg tgatctggcg ctggatgacc ttgttatctt caccgacggc cagaacgtaa ttgccgctct ggtcggtcat gattgccttt tgcgggacga gcagggcttc aacggggtgt ttgctcgccg ccgttacctg aacatactgc ccgtcgatca ggacattgtt cggattggga aactgtgccc ggaccatcag cgtatcggta ttgggattaa tcttgatccc ggtaaagtcg atggcccccg tttgcggata tgtcatgccg ttggagagcg tcaggctgac ggctacattg ctgcggtctg tggccgttct cccgccttcg gttaaatcga gaatggtctt gtcgctgacg gggaaggtga cgtaaatcgg gtcctggctg acgatcgttg ccagagggcc tgaggacggc ccgacaaggg cgccgacgct gaaattggcc atgccgatgc gtccggaaaa aggcgcgcgt atatcggtat agccgagatt gatttgcgcc tgttccagcg aagcctgcgc ctgctgcacg acggccaaag cctgcttttc cgctgccaca cgatcatcaa gcatggcttg cgaaacggtc ttctgcttgt agagcgagcg ggcgcgctcg gtctggagtt tcaggttgtc ggcatcggcc tgcgcttttg caagattggc ctgcgccgca tccagcgccg cctgaagcgc atccttttgc agggcaaaca gcaacatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

MAHHHHHHML FALQKDALQA ALDGAQANLA KAQADADNLK LQTERARSLY KQKTVSQAML DDRVAAEKQA LAVVQQAQAS LEQAQINLGY TDIRAPFSGR IGMANFSVGA LVGPSSGPLA TIVSQDPIYV TFPVSDKTIL DLTEGGRTAT DRSNVAVSLT LSNGMTYPQT GAIDFTGIKI NPNTDTLMVR AQFPNPNNVL IDGQYVQVTA ASKYPVEALL VPQKAIMTDQ SGNYVLAVGE DNKVIQRQIT QGSTFGSNVV VKSGLAVGDQ VVVDGLQRIR PGQKVDPQIV DATTPAQSHV GGELIHAVIR LH

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgctgt ttgccctgca aaaggatgcg cttcaggcgg cgctggatgg ggcgcaggcc aatcttgcaa aagcgcaggc cgatgccgac aacctgaaac tccagaccga gcgcgcccgc tcgctctaca agcagaagac cgtttcgcaa gccatgcttg atgatcgtgt ggcagcggaa aagcaggctt tggccgtcgt gcagcaggcg caggcttcgc tggaacaggc gcaaatcaat ctcggctata ccgatatacg cgcgcctttt tccggacgca tcggcatggc caatttcagc gtcggcgccc ttgtcgggcc gtcctcaggc cctctggcaa cgatcgtcag ccaggacccg atttacgtca ccttccccgt cagcgacaag accattctcg atctaaccga aggcgggaga acggccacag accgcagcaa tgtagccgtc agcctgacgc tctccaacgg catgacatat ccgcaaacgg gggccatcga ctttaccggg atcaagatta atcccaatac cgatacgctg atggtccggg cacagtttcc caatccgaac aatgtcctga tcgacgggca gtatgttcag gtaacggcgg cgagcaaata ccccgttgaa gccctgctcg tcccgcaaaa ggcaatcatg accgaccaga gcggcaatta cgttctggcc gtcggtgaag ataacaaggt catccagcgc cagatcacgc aaggttcgac ctttggcagc aatgttgtgg tcaagtccgg cctcgcggtg ggcgatcagg tcgtggtcga tggcctccag cgcatccggc ccggccagaa ggtcgatccg cagatcgtcg atgcaaccac gcccgcccaa agccatgtcg gtggggaatt gatccatgct gtcatccgtc ttcattgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat