SSGCID: Target Page

BrsuB.00076.a

Uracil phosphoribosyltransferase (EC 2.4.2.9)

CENTER ID:	BrsuB.00076.a
ORGANISM:	Brucella suis ATCC 23445
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuB.00076.a.B1.GE37113	Full length( BrsuB.00076.a )		1	208

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruSui83806_1126
BV-BRC:	fig\|470137.4.peg.1126
RefSeq:	YP_001622833.1
UniProt:	A9WW75

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGVTVVSHPL VQHKLTIMRK KETSTASFQR LLKEISLLLC YEVTRNLELT TMSIETPLMP MEAPVLEGKK LVFASILRAG NGLLEGMLDL VPAARVAHIG LYRDHDTLQP IEYYFKAPED IVNRLVIVVD PMLATANSAI AAIDKLKERG ATNIRFLCLL AAPEGIERFT KAHPDVEVFT ASIDERLDEK GYIVPGLGDA GDRMYGTK

NT Sequence

ATGGGCGTTA CAGTCGTCAG CCATCCGCTT GTCCAGCACA AGCTTACCAT CATGCGCAAG AAGGAGACGT CCACGGCCAG CTTCCAGCGG TTGCTGAAGG AGATTTCGCT GTTGCTGTGC TACGAAGTGA CGCGCAATCT CGAACTTACC ACCATGTCTA TTGAAACCCC GCTGATGCCG ATGGAAGCCC CGGTGCTGGA AGGCAAGAAG CTTGTGTTCG CTTCCATCCT GCGTGCAGGT AACGGCCTTC TGGAAGGGAT GCTCGATCTG GTGCCTGCCG CGCGCGTCGC CCATATCGGC CTCTACCGCG ACCACGACAC GCTTCAGCCG ATCGAATATT ACTTCAAGGC ACCGGAAGAT ATCGTCAATC GCCTCGTTAT CGTTGTCGAT CCGATGCTTG CCACCGCCAA CTCGGCCATT GCCGCTATCG ACAAGCTGAA AGAGCGCGGC GCAACCAATA TCCGCTTCCT CTGCCTGCTG GCCGCACCCG AAGGCATTGA ACGCTTCACC AAGGCGCATC CGGATGTCGA GGTCTTCACC GCCTCCATCG ATGAACGCCT TGATGAAAAA GGCTATATTG TTCCGGGCCT CGGCGACGCG GGCGACCGCA TGTACGGCAC GAAA

Details for BrsuB.00076.a.B1.GE37113

HARVESTED ON:	7/17/2013
SEQUENCED ON:	7/20/2013
EXPECTED MW:	24kDa
OBSERVED MW:	24kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG VTVVSHPLVQ HKLTIMRKKE TSTASFQRLL KEISLLLCYE VTRNLELTTM SIETPLMPME APVLEGKKLV FASILRAGNG LLEGMLDLVP AARVAHIGLY RDHDTLQPIE YYFKAPEDIV NRLVIVVDPM LATANSAIAA IDKLKERGAT NIRFLCLLAA PEGIERFTKA HPDVEVFTAS IDERLDEKGY IVPGLGDAGD RMYGTK

Validated NT Sequence

atcttactca cttatttcgt gccgtacatg cggtcgcccg cgtcgccgag gcccggaaca atatagcctt tttcatcaag gcgttcatcg atggaggcgg tgaagacctc gacatccgga tgcgccttgg tgaagcgttc aatgccttcg ggtgcggcca gcaggcagag gaagcggata ttggttgcgc cgcgctcttt cagcttgtcg atagcggcaa tggccgagtt ggcggtggca agcatcggat cgacaacgat aacgaggcga ttgacgatat cttccggtgc cttgaagtaa tattcgatcg gctgaagcgt gtcgtggtcg cggtagaggc cgatatgggc gacgcgcgcg gcaggcacca gatcgagcat cccttccaga aggccgttac ctgcacgcag gatggaagcg aacacaagct tcttgccttc cagcaccggg gcttccatcg gcatcagcgg ggtttcaata gacatggtgg taagttcgag attgcgcgtc acttcgtagc acagcaacag cgaaatctcc ttcagcaacc gctggaagct ggccgtggac gtctccttct tgcgcatgat ggtaagcttg tgctggacaa gcggatggct gacgactgta acgcccatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggcg ttacagtcgt cagccatccg cttgtccagc acaagcttac catcatgcgc aagaaggaga cgtccacggc cagcttccag cggttgctga aggagatttc gctgttgctg tgctacgaag tgacgcgcaa tctcgaactt accaccatgt ctattgaaac cccgctgatg ccgatggaag ccccggtgct ggaaggcaag aagcttgtgt tcgcttccat cctgcgtgca ggtaacggcc ttctggaagg gatgctcgat ctggtgcctg ccgcgcgcgt cgcccatatc ggcctctacc gcgaccacga cacgcttcag ccgatcgaat attacttcaa ggcaccggaa gatatcgtca atcgcctcgt tatcgttgtc gatccgatgc ttgccaccgc caactcggcc attgccgcta tcgacaagct gaaagagcgc ggcgcaacca atatccgctt cctctgcctg ctggccgcac ccgaaggcat tgaacgcttc accaaggcgc atccggatgt cgaggtcttc accgcctcca tcgatgaacg ccttgatgaa aaaggctata ttgttccggg cctcggcgac gcgggcgacc gcatgtacgg cacgaaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at