SSGCID: Target Page

BrsuB.00057.i

Methionine ABC transporter ATP-binding protein

CENTER ID:	BrsuB.00057.i
ORGANISM:	Brucella suis ATCC 23445
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuB.00057.i.B1.GE37111	Full length( BrsuB.00057.i )		1	287

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruSui83806_2636
BV-BRC:	fig\|470137.4.peg.2636
RefSeq:	YP_001627692.1
UniProt:	B0CGH1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVMENGIDTQ SQPQEDQNHQ TGVPMISVRD VTVSFGHQTV LDKLSLDIFQ GEVLGFIGPS GSGKSVLMRT ILGLNKKQTG DIEILGRNID KLNEMEKMAI DMRMGVLFQH GALFSALNVL ENIQVPMREY LDLSPKLMDE LARLKVDLVG LHPDTAEKFP SELSGGMIKR AALARALALD PDIVFLDEPT SGLDPIGAAE FDDLIANLRD TMGLTVYMVT HDLDSLFAIC DRIAVLGNKK VLVSGTIEDM LKVDDPWVKA YFRGKRARQI DTSAPSRRRP GNRLQTG

NT Sequence

ATGGTTATGG AAAACGGCAT CGACACTCAG TCGCAACCCC AGGAAGATCA GAACCACCAG ACGGGCGTGC CGATGATCTC GGTGCGCGAC GTTACGGTTT CGTTTGGTCA CCAGACTGTT CTCGACAAGC TTTCGCTCGA TATATTCCAG GGAGAGGTCC TTGGCTTTAT CGGGCCGTCC GGCTCCGGCA AGTCGGTGCT GATGCGTACC ATTCTTGGCC TCAACAAGAA GCAGACCGGC GATATCGAAA TTCTCGGGCG CAATATCGAC AAGCTGAACG AGATGGAAAA GATGGCCATC GATATGCGCA TGGGGGTTCT CTTTCAGCAT GGCGCGCTGT TTTCCGCGCT GAACGTGCTG GAGAACATTC AGGTGCCGAT GCGTGAATAT CTCGACCTTT CGCCCAAACT CATGGATGAG CTGGCGCGCC TGAAGGTCGA TCTGGTGGGA TTGCACCCGG ATACGGCGGA AAAATTTCCC TCCGAACTTT CAGGCGGCAT GATCAAGCGC GCCGCGCTTG CACGCGCGCT GGCGCTGGAT CCGGATATTG TGTTTCTTGA TGAGCCGACA TCCGGCCTGG ACCCGATCGG TGCAGCGGAA TTTGACGATC TGATCGCAAA CCTGCGCGAT ACGATGGGGC TGACGGTTTA CATGGTGACC CATGACCTCG ACAGTCTTTT TGCAATCTGC GACCGGATCG CGGTTCTGGG AAACAAGAAG GTGCTCGTGA GCGGTACCAT TGAGGACATG CTGAAGGTGG ATGACCCTTG GGTGAAGGCC TATTTTCGGG GCAAGCGTGC GCGTCAGATT GATACATCTG CGCCAAGTAG ACGGCGGCCG GGCAACCGCT TGCAGACTGG A

Details for BrsuB.00057.i.B1.GE37111

HARVESTED ON:	7/17/2013
SEQUENCED ON:	7/20/2013
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	97
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMV MENGIDTQSQ PQEDQNHQTG VPMISVRDVT VSFGHQTVLD KLSLDIFQGE VLGFIGPSGS GKSVLMRTIL GLNKKQTGDI EILGRNIDKL NEMEKMAIDM RMGVLFQHGA LFSALNVLEN IQVPMREYLD LSPKLMDELA RLKVDLVGLH PDTAEKFPSE LSGGMIKRAA LARALALDPD IVFLDEPTSG LDPIGAAEFD DLIANLRDTM GLTVYMVTHD LDSLFAICDR IAVLGNKKVL VSGTIXDMLX XDDPWVKAYF RGKRARQXDT SAPSXXXPXN RLQTG

Validated NT Sequence

annnattttn tttactttaa gaaggagata taccatggct caccaccacc accaccatat ggttatggaa aacggcatcg acactcagtc gcaaccccag gaagatcaga accaccagac gggcgtgccg atgatctcgg tgcgcgacgt tacggtttcg tttggtcacc agactgttct cgacaagctt tcgctcgata tattccaggg agaggtcctt ggctttatcg ggccgtccgg ctccggcaag tcggtgctga tgcgtaccat tcttggcctc aacaagaagc agaccggcga tatcgaaatt ctcgggcgca atatcgacaa gctgaacgag atggaaaaga tggccatcga tatgcgcatg ggggttctct ttcagcatgg cgcgctgttt tccgcgctga acgtgctgga gaacattcag gtgccgatgc gtgaatatct cgacctttcg cccaaactca tggatgagct ggcgcgcctg aaggtcgatc tggtgggatt gcacccggat acggcggaaa aatttccctc cgaactttca ggcggcatga tcaagcgcgc cgcgcttgca cgcgcgctgg cgctggatcc ggatattgtg tttcttgatg agccgacatc cggcctggac ccgatcggtg cagcggaatt tgacgatctg atcgcaaacc tgcgcgatac gatggggctg acggtttaca tggtgaccca tgacctcgac agtctttttg caatctgcga ccggatcgcg gttctgggaa acaagaaggt gctcgtgagc ggcaccattg angacatgct gnanntggat gacccttggg tgaaggccta ttttcggggc aagcgtgcgc gtcagantga tacatctgcg ccaagtanan ggnggccggn naaccgcttg cagactggnt aagtgagtaa nanaggancn nnnnnnnnnn ncccc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtta tggaaaacgg catcgacact cagtcgcaac cccaggaaga tcagaaccac cagacgggcg tgccgatgat ctcggtgcgc gacgttacgg tttcgtttgg tcaccagact gttctcgaca agctttcgct cgatatattc cagggagagg tccttggctt tatcgggccg tccggctccg gcaagtcggt gctgatgcgt accattcttg gcctcaacaa gaagcagacc ggcgatatcg aaattctcgg gcgcaatatc gacaagctga acgagatgga aaagatggcc atcgatatgc gcatgggggt tctctttcag catggcgcgc tgttttccgc gctgaacgtg ctggagaaca ttcaggtgcc gatgcgtgaa tatctcgacc tttcgcccaa actcatggat gagctggcgc gcctgaaggt cgatctggtg ggattgcacc cggatacggc ggaaaaattt ccctccgaac tttcaggcgg catgatcaag cgcgccgcgc ttgcacgcgc gctggcgctg gatccggata ttgtgtttct tgatgagccg acatccggcc tggacccgat cggtgcagcg gaatttgacg atctgatcgc aaacctgcgc gatacgatgg ggctgacggt ttacatggtg acccatgacc tcgacagtct ttttgcaatc tgcgaccgga tcgcggttct gggaaacaag aaggtgctcg tgagcggtac cattgaggac atgctgaagg tggatgaccc ttgggtgaag gcctattttc ggggcaagcg tgcgcgtcag attgatacat ctgcgccaag tagacggcgg ccgggcaacc gcttgcagac tggatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat