BrsuB.00057.f

HIGH-AFFINITY BRANCHED-CHAIN AMINO ACID TRANSPORT SYSTEM PERMEASE PROTEIN LIVM / HIGH-AFFINITY BRANCHED-CHAIN AMINO ACID TRANSPORT ATP-BINDING PROTEIN LIVG

CENTER ID: BrsuB.00057.f
ORGANISM: Brucella suis ATCC 23445
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuB.00057.f.B1.GE37108 Full length( BrsuB.00057.f ) 1 235
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruSui83806_1489
BV-BRC: fig|470137.4.peg.1489
RefSeq: YP_001623147.1
UniProt: A9WXA9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLEVRDLTVR YGAVTALNKV NLTVPAGTVV GLVGPNGAGK STLVDAIAGF LPRYDGTVLL HGKPLEALSA TARAKAGIRR TWQTTRIAPE LRVGDYIRLA AGPIGQTELE SLLGWFGCPP PETPIASVDA GTRRLLDVAG AVASRAPVIL LDEPAAGISY DEALKLGERI AAIPATFGSA VLLIEHDMDL VRRACTAITV FDFGQVIASG EPAEVLDMPE VQKVYMGVDF EEAAE
NT Sequence
GTGCTTGAAG TCCGCGATCT CACCGTGCGC TATGGTGCGG TGACGGCCTT GAACAAGGTC AACCTCACGG TGCCAGCCGG TACGGTTGTG GGCCTTGTCG GGCCGAACGG AGCGGGCAAA TCGACATTGG TGGACGCCAT TGCCGGGTTC CTGCCGCGCT ATGACGGTAC GGTTCTTCTG CATGGAAAGC CGCTTGAAGC GCTTTCGGCA ACGGCGCGGG CCAAGGCTGG CATTCGCCGC ACCTGGCAGA CAACACGCAT CGCGCCGGAA TTGCGGGTTG GCGACTATAT TCGCCTTGCC GCCGGTCCCA TAGGACAGAC GGAACTGGAA AGCCTGCTTG GCTGGTTCGG TTGTCCGCCG CCCGAGACGC CGATTGCTTC CGTCGATGCG GGAACGCGCC GCCTGCTCGA TGTTGCGGGT GCTGTCGCCT CGCGCGCGCC GGTGATCCTG CTCGATGAGC CAGCCGCTGG CATTTCCTAT GACGAAGCCT TGAAATTGGG CGAGCGGATC GCCGCCATCC CAGCCACCTT CGGCAGTGCC GTCCTGTTGA TCGAGCATGA CATGGATCTC GTGCGGCGGG CCTGCACGGC CATTACCGTG TTCGATTTCG GACAGGTGAT CGCTTCGGGC GAACCGGCGG AAGTGCTGGA CATGCCAGAA GTACAGAAAG TCTATATGGG CGTTGATTTC GAGGAGGCTG CGGAA
Details for BrsuB.00057.f.B1.GE37108
HARVESTED ON: 7/17/2013
SEQUENCED ON: 7/20/2013
EXPECTED MW: 26kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV LEVRDLTVRY GAVTALNKVN LTVPAGTVVG LVGPNGAGKS TLVDAIAGFL PRYDGTVLLH GKPLEALSAT ARAKAGIRRT WQTTRIAPEL RVGDYIRLAA GPIGQTELES LLGWFGCPPP ETPIASVDAG TRRLLDVAGA VASRAPVILL DEPAAGISYD EALKLGERIA AIPATFGSAV LLIEHDMDLV RRACTAITVF DFGQVIASGE PAEVLDMPEV QKAYMGVDFE EAAE
Validated NT Sequence
atcttactca cttattccgc agcctcctcg aaatcaacgc ccatataggc tttctgtact tctggcatgt ccagcacttc cgccggttcg cccgaagcga tcacctgtcc gaaatcgaac acggtaatgg ccgtgcaggc ccgccgcacg agatccatgt catgctcgat caacaggacg gcactgccga aggtggctgg gatggcggcg atccgctcgc ccaatttcaa ggcttcgtca taggaaatgc cagcggctgg ctcatcgagc aggatcaccg gcgcgcgcga ggcgacagca cccgcaacat cgagcaggcg gcgcgttccc gcatcgacgg aagcaatcgg cgtctcgggc ggcggacaac cgaaccagcc aagcaggctt tccagttccg tctgtcctat gggaccggcg gcaaggcgaa tatagtcgcc aacccgcaat tccggcgcga tgcgtgttgt ctgccaggtg cggcgaatgc cagccttggc ccgcgccgtt gccgaaagcg cttcaagcgg ctttccatgc agaagaaccg taccgtcata gcgcggcagg aacccggcaa tggcgtccac caatgtcgat ttgcccgctc cgttcggccc gacaaggccc acaaccgtac cggctggcac cgtgaggttg accttgttca aggccgtcac cgcaccatag cgcacggtga gatcgcggac ttcaagcacc atatggtggt ggtggtggtg agccatggta t
Expected Protein Sequence
MAHHHHHHML EVRDLTVRYG AVTALNKVNL TVPAGTVVGL VGPNGAGKST LVDAIAGFLP RYDGTVLLHG KPLEALSATA RAKAGIRRTW QTTRIAPELR VGDYIRLAAG PIGQTELESL LGWFGCPPPE TPIASVDAGT RRLLDVAGAV ASRAPVILLD EPAAGISYDE ALKLGERIAA IPATFGSAVL LIEHDMDLVR RACTAITVFD FGQVIASGEP AEVLDMPEVQ KVYMGVDFEE AAE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgcttg aagtccgcga tctcaccgtg cgctatggtg cggtgacggc cttgaacaag gtcaacctca cggtgccagc cggtacggtt gtgggccttg tcgggccgaa cggagcgggc aaatcgacat tggtggacgc cattgccggg ttcctgccgc gctatgacgg tacggttctt ctgcatggaa agccgcttga agcgctttcg gcaacggcgc gggccaaggc tggcattcgc cgcacctggc agacaacacg catcgcgccg gaattgcggg ttggcgacta tattcgcctt gccgccggtc ccataggaca gacggaactg gaaagcctgc ttggctggtt cggttgtccg ccgcccgaga cgccgattgc ttccgtcgat gcgggaacgc gccgcctgct cgatgttgcg ggtgctgtcg cctcgcgcgc gccggtgatc ctgctcgatg agccagccgc tggcatttcc tatgacgaag ccttgaaatt gggcgagcgg atcgccgcca tcccagccac cttcggcagt gccgtcctgt tgatcgagca tgacatggat ctcgtgcggc gggcctgcac ggccattacc gtgttcgatt tcggacaggt gatcgcttcg ggcgaaccgg cggaagtgct ggacatgcca gaagtacaga aagtctatat gggcgttgat ttcgaggagg ctgcggaatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat