SSGCID: Target Page

BrsuB.00057.d

Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1)

CENTER ID:	BrsuB.00057.d
ORGANISM:	Brucella suis ATCC 23445
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuB.00057.d.B1.GE37106	Full length( BrsuB.00057.d )		1	202

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruSui83806_1172

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDSQKISRDS GLGISIEGVT KKYGSFTAVD GLDMQIAPGE FLALLGPSGS GKSTILMLLA GFERPDNGRI LFGNNDYTRV PPHRRNIGMV FQHYTLFPNM SVLDNVAFPL KTRGMERSQR HRIAREALAR VRLADFADRS PKQLSGGQQQ RVALARSIVY SPRVLLMDEP LSALDKNPAR RDADRDQALA YRAWHDDRIR DP

NT Sequence

ATGGATAGCC AAAAGATCTC CCGCGATTCA GGCTTGGGTA TCAGCATTGA AGGCGTCACC AAGAAATATG GCAGCTTCAC CGCAGTCGAC GGGCTGGATA TGCAGATTGC GCCTGGCGAA TTTCTGGCGT TGCTGGGGCC TTCCGGATCG GGAAAGTCCA CCATCCTGAT GCTGCTGGCG GGGTTCGAGC GCCCGGATAA TGGCCGCATT CTCTTCGGCA ATAATGATTA TACCCGTGTT CCGCCGCACA GGCGCAATAT TGGCATGGTA TTCCAGCATT ACACCCTGTT TCCCAATATG AGCGTGCTCG ATAATGTGGC GTTCCCGCTC AAGACGCGGG GCATGGAGCG CAGCCAGCGC CACAGGATCG CGCGTGAAGC GCTGGCGCGC GTGCGGCTTG CCGATTTTGC GGATCGCAGT CCCAAGCAAT TGTCCGGCGG GCAGCAGCAA CGCGTCGCGC TGGCACGCTC CATTGTCTAT TCGCCGCGCG TGCTCCTGAT GGATGAGCCA CTTTCGGCCC TGGACAAGAA CCCTGCGCGA AGAGATGCAG ATCGAGATCA AGCGCTTGCA TACCGAGCTT GGCATGACGA TCGTATTCGT GACCCA

Details for BrsuB.00057.d.B1.GE37106

HARVESTED ON:	7/17/2013
SEQUENCED ON:	7/20/2013
EXPECTED MW:	24kDa
OBSERVED MW:	24kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	90
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMD SQKISRDSGL GISIEGVTKK YGSFTAVDGL DMQIAPGEFL ALLGPSGSGK STILMLLAGF EHPDNGRILF GNNDYTRVPP HRRNIGMVFQ HYTLFPNMSV LDNVAFPLKT RGMERSQRHR IAREALARVR LADFADRSPK QLSGGQQQRV ALARSIVYSP RVLLMDEPLS ALDKNLREEM QIEIKRLHTE LGMTIVFVTH K

Validated NT Sequence

ttttgtttaa ctttaagaag gagatatacc atggctcacc accaccacca ccatatggat agccaaaaga tctcccgcga ttcaggcttg ggtatcagca ttgaaggcgt caccaagaaa tatggcagct tcaccgcagt cgacgggctg gatatgcaga ttgcgcctgg cgaatttctg gcgttgctgg ggccttccgg atcgggaaag tccaccatcc tgatgctgct ggcggggttc gagcacccgg ataatggccg cattctcttc ggcaataatg attatacccg tgttccgccg cacaggcgca atattggcat ggtattccag cattacaccc tgtttcccaa tatgagcgtg ctcgataatg tggcgttccc gctcaagacg cggggcatgg agcgcagcca gcgccacagg atcgcgcgtg aagcgctggc gcgcgtgcgg cttgccgatt ttgcggatcg cagtcccaag caattgtccg gcgggcagca gcaacgcgtc gcgctggcac gctccattgt ctattcgccg cgcgtgctcc tgatggatga gccgctttcg gccctggaca agaacctgcg cgaagagatg cagatcgaga tcaagcgctt gcataccgag cttggcatga cgatcgtatt cgtgacccat aagtgagtaa gataggatcc ggctgctaac annccccnna nnnnnnnnnn aaannnttna ngtgnccncn nnnnnnncgt ccnnncnnnn nnnncntccn nannnnnngg nntatcgnan nnnnctctnc nnnnnngnnn cngcnnnt

Expected Protein Sequence

MAHHHHHHMD SQKISRDSGL GISIEGVTKK YGSFTAVDGL DMQIAPGEFL ALLGPSGSGK STILMLLAGF ERPDNGRILF GNNDYTRVPP HRRNIGMVFQ HYTLFPNMSV LDNVAFPLKT RGMERSQRHR IAREALARVR LADFADRSPK QLSGGQQQRV ALARSIVYSP RVLLMDEPLS ALDKNPARRD ADRDQALAYR AWHDDRIRDP

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggata gccaaaagat ctcccgcgat tcaggcttgg gtatcagcat tgaaggcgtc accaagaaat atggcagctt caccgcagtc gacgggctgg atatgcagat tgcgcctggc gaatttctgg cgttgctggg gccttccgga tcgggaaagt ccaccatcct gatgctgctg gcggggttcg agcgcccgga taatggccgc attctcttcg gcaataatga ttatacccgt gttccgccgc acaggcgcaa tattggcatg gtattccagc attacaccct gtttcccaat atgagcgtgc tcgataatgt ggcgttcccg ctcaagacgc ggggcatgga gcgcagccag cgccacagga tcgcgcgtga agcgctggcg cgcgtgcggc ttgccgattt tgcggatcgc agtcccaagc aattgtccgg cgggcagcag caacgcgtcg cgctggcacg ctccattgtc tattcgccgc gcgtgctcct gatggatgag ccactttcgg ccctggacaa gaaccctgcg cgaagagatg cagatcgaga tcaagcgctt gcataccgag cttggcatga cgatcgtatt cgtgacccat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat