BrsuB.00057.c

ABC transporter, ATP-binding protein

CENTER ID: BrsuB.00057.c
ORGANISM: Brucella suis ATCC 23445
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuB.00057.c.B1.GE37105 Full length( BrsuB.00057.c ) 1 355
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruSui83806_0843
BV-BRC: fig|470137.4.peg.843
RefSeq: YP_001622587.1
UniProt: A9WZ99

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQAASLPFNE IRLERMSRAF GSHNALKSIT LSISKGEFIA LLGPSGCGKS TALNCIAGLL ATTGGGIYID EKRIDTLKPE DRGFGMVFQN YALFPHMSVR ENVGFGLKMR GTPKSEIRKR VDEAIGMVRL NGQEEKLPGQ LSGGQQQRVA IARAIVIEPP LVLMDEPLSN LDAKLRLEMR AEIRRIHNQL GSTTIYVTHD QDEALSLADR IVVLRDGEIR QVGVPSDLYE RPDHLDVAEF MGYRNRLPGK VTAIDGERAT ISIGGSLIAG IARVPLAVGQ DVVLAARPDD VATGPTSPAA LQAKVETIEY RGRDFVGTAN MSDGHNLVFH ASAHAELGSA VSLTVDPNRA LIFPA
NT Sequence
ATGCAAGCAG CATCTTTGCC CTTCAATGAA ATTCGGCTCG AAAGGATGAG CCGTGCTTTT GGGTCACATA ATGCACTCAA AAGCATTACG CTGTCGATCA GCAAGGGTGA GTTCATTGCA TTGCTGGGCC CTTCGGGCTG CGGAAAATCA ACGGCTTTGA ACTGTATTGC CGGTCTGCTG GCTACCACCG GTGGCGGCAT CTATATCGAT GAAAAGCGCA TCGATACATT GAAGCCGGAA GACCGTGGCT TTGGGATGGT GTTTCAGAAT TATGCGCTGT TTCCACATAT GAGCGTCCGC GAGAATGTGG GCTTCGGCCT GAAAATGCGT GGCACACCGA AGTCAGAAAT CCGAAAACGT GTAGATGAAG CCATAGGAAT GGTTCGTCTC AATGGTCAGG AAGAAAAACT GCCCGGCCAG CTTTCAGGCG GTCAGCAACA ACGGGTTGCA ATTGCCCGCG CCATCGTCAT CGAACCGCCA TTGGTTCTTA TGGATGAGCC GCTTTCCAAT CTCGATGCCA AACTGCGCCT GGAAATGCGG GCCGAAATTC GCCGCATTCA CAATCAGCTT GGCTCGACCA CGATTTATGT CACGCACGAT CAGGATGAGG CGCTCTCGCT TGCAGATCGT ATAGTGGTGC TGCGCGATGG CGAGATCCGC CAGGTTGGCG TGCCCAGCGA TCTTTATGAG CGTCCCGACC ATTTGGACGT CGCCGAATTT ATGGGATATC GCAATCGCCT CCCCGGTAAG GTGACGGCAA TTGATGGAGA GCGGGCAACT ATATCCATCG GCGGTTCGCT GATTGCAGGT ATTGCCCGCG TACCTCTTGC CGTAGGTCAG GATGTCGTGC TTGCAGCGCG CCCCGATGAC GTCGCTACGG GACCTACAAG CCCAGCGGCC CTTCAAGCAA AAGTCGAGAC GATCGAATAT CGCGGACGTG ATTTTGTGGG CACCGCGAAC ATGTCAGACG GGCATAATCT TGTGTTTCAC GCCTCCGCCC ACGCTGAACT GGGAAGTGCA GTTTCTCTGA CGGTCGACCC AAACCGTGCC CTGATTTTCC CGGCG
Details for BrsuB.00057.c.B1.GE37105
HARVESTED ON: 7/17/2013
SEQUENCED ON: 7/20/2013
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 87
PERCENT COVERAGE: 102
Validated AA Sequence
MAHHHHHHMQ AASLPFNEIR LERMSRAFGS HNALKSITLS ISKGEFIALL GPSGCGKSTA LNCIAGLLAT TGGGIYIDEK RIDTLKPEDR GFGMVFQNYA LFPHMSVREN VGFGLKMRGT PKSEIRKRVD EAIGMVRLNG QEEKLPGQLS GGQQQRVAIA RAIVIEPPLV LMDEPLSNLD AKLRLEMRAE IRRIHNQLGS TTIYVTHDQD EALSLADRIV VLRDGEIRQV GVPSDLYERP DHLDVAEFMG YRNRLSGKVT AXDGERATIS IGGSLIAGIA RVPLAVXXXV VLAARPDDVA TGPASPAALQ AKVETIEYRX XDFVGXXXXS XGXXXFXPXP XXXXXXFXXX RPXXXXXXXX XXXXXXXXXX XX
Validated NT Sequence
tttgtnnnnt ttaagaagga ganataccat ggctcaccac caccaccacc atatgcaagc agcatctttg cccttcaatg aaattcggct cgaaaggatg agccgtgctt ttgggtcaca taatgcactc aaaagcatta cgctgtcgat cagcaagggt gagttcattg cattgctggg cccttcgggc tgcggaaaat caacggcttt gaactgtatt gccggtctgc tggctaccac cggtggcggc atctatatcg atgaaaagcg catcgataca ttgaagccgg aagaccgtgg ctttgggatg gtgtttcaga attatgcgct gtttccacat atgagcgtcc gcgagaatgt gggcttcggc ctgaaaatgc gtggcacacc gaagtcagaa atccgaaaac gtgtagatga agccatagga atggttcgtc tcaatggtca ggaagaaaaa ctgcccggcc agctttcagg cggtcagcaa caacgggttg caattgcccg cgccatcgtc atcgaaccgc cattggttct tatggatgag ccgctttcca atctcgatgc aaaactgcgc ctggaaatgc gggccgaaat tcgccgcatt cacaatcagc ttggctcgac cacgatttat gtcacgcacg atcaggatga ggcgctctcg cttgcagatc gtatagtggt gctgcgcgat ggcgagatcc gccaggttgg cgtgcccagc gatctttatg agcgtcccga ccatttggac gtcgccgaat ttatgggata tcgcaatcgc ctctccggta aggtgacggc anttgatgga gagcgggcaa ctatatccat cggcggttcg ctgattgcag gtattgcccg cgtacctctt gccgtagntc nnnatgtcgt gcttgcagcg cgccccgatg acgtcgctac gggacctgca agcccagcgg cccttcaagc aaaagtcgag acgatcgaat atcgcgnang tgattttgtg ggcnncncga nnntgtcnna nggnnanann ngtttcangc cnnnnccnnn ctnannngnn nngcantttn nnnngagncg acccnaancn nnnnctgnnn ncnnnnnann nannannnnn nncnnnnnnn nnnnnnnnnn nnnna
Expected Protein Sequence
MAHHHHHHMQ AASLPFNEIR LERMSRAFGS HNALKSITLS ISKGEFIALL GPSGCGKSTA LNCIAGLLAT TGGGIYIDEK RIDTLKPEDR GFGMVFQNYA LFPHMSVREN VGFGLKMRGT PKSEIRKRVD EAIGMVRLNG QEEKLPGQLS GGQQQRVAIA RAIVIEPPLV LMDEPLSNLD AKLRLEMRAE IRRIHNQLGS TTIYVTHDQD EALSLADRIV VLRDGEIRQV GVPSDLYERP DHLDVAEFMG YRNRLPGKVT AIDGERATIS IGGSLIAGIA RVPLAVGQDV VLAARPDDVA TGPTSPAALQ AKVETIEYRG RDFVGTANMS DGHNLVFHAS AHAELGSAVS LTVDPNRALI FPA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaag cagcatcttt gcccttcaat gaaattcggc tcgaaaggat gagccgtgct tttgggtcac ataatgcact caaaagcatt acgctgtcga tcagcaaggg tgagttcatt gcattgctgg gcccttcggg ctgcggaaaa tcaacggctt tgaactgtat tgccggtctg ctggctacca ccggtggcgg catctatatc gatgaaaagc gcatcgatac attgaagccg gaagaccgtg gctttgggat ggtgtttcag aattatgcgc tgtttccaca tatgagcgtc cgcgagaatg tgggcttcgg cctgaaaatg cgtggcacac cgaagtcaga aatccgaaaa cgtgtagatg aagccatagg aatggttcgt ctcaatggtc aggaagaaaa actgcccggc cagctttcag gcggtcagca acaacgggtt gcaattgccc gcgccatcgt catcgaaccg ccattggttc ttatggatga gccgctttcc aatctcgatg ccaaactgcg cctggaaatg cgggccgaaa ttcgccgcat tcacaatcag cttggctcga ccacgattta tgtcacgcac gatcaggatg aggcgctctc gcttgcagat cgtatagtgg tgctgcgcga tggcgagatc cgccaggttg gcgtgcccag cgatctttat gagcgtcccg accatttgga cgtcgccgaa tttatgggat atcgcaatcg cctccccggt aaggtgacgg caattgatgg agagcgggca actatatcca tcggcggttc gctgattgca ggtattgccc gcgtacctct tgccgtaggt caggatgtcg tgcttgcagc gcgccccgat gacgtcgcta cgggacctac aagcccagcg gcccttcaag caaaagtcga gacgatcgaa tatcgcggac gtgattttgt gggcaccgcg aacatgtcag acgggcataa tcttgtgttt cacgcctccg cccacgctga actgggaagt gcagtttctc tgacggtcga cccaaaccgt gccctgattt tcccggcgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat