SSGCID: Target Page

BrsuB.00050.a

UDP-glucose 4-epimerase (EC 5.1.3.2)

CENTER ID:	BrsuB.00050.a
ORGANISM:	Brucella suis ATCC 23445
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuB.00050.a.B1.GE37978	Full length( BrsuB.00050.a )		1	336

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruSui83806_0889
BV-BRC:	fig\|470137.4.peg.889
RefSeq:	YP_001622626.1
UniProt:	A9WZD8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRHIIFGGDG FVGRYLAPQL LADGHEVIVA DIVKSDLAHY GDATFVHTDV TDPESIRKLG IRADDMVYNL SAKMLSPIQV RAKRHDFFFP VNYYGTENII KAMDAAGAKK LVHFTTDMLY GHTYVWPQKE DHPCKPLGEY GLSKLKTEEL AAQWRTRGMN ISLFRPRLII GPGRLGILEK LFKLIDNNLP VPMIGSGKNP YQFISVFDCA SAARAAFKAG VPNEVYNLGS LNPPPVRKLL GDLIKHAGSK SMLLPTPAWA VKRTLDFLDW LNMPLMDPEQ YLIADEMCVL DVSKGERELG WIPQYRDEDM LIAAYSEYRK KKAGGAENRL SPSKAA

NT Sequence

ATGAGGCATA TTATTTTCGG CGGTGACGGT TTTGTCGGGC GTTATCTCGC GCCCCAATTG CTGGCCGATG GCCATGAGGT GATCGTCGCC GATATCGTCA AAAGCGATCT TGCGCATTAT GGCGATGCGA CCTTCGTTCA TACGGATGTG ACGGACCCGG AATCGATCCG CAAGCTTGGC ATTCGCGCCG ATGACATGGT TTATAATCTC TCGGCCAAGA TGCTTTCGCC CATTCAGGTC CGCGCCAAGC GGCATGATTT CTTCTTCCCC GTGAACTATT ACGGCACAGA GAACATCATA AAGGCGATGG ATGCAGCGGG GGCAAAAAAG CTGGTCCATT TCACCACCGA TATGCTCTAT GGCCACACCT ATGTCTGGCC GCAAAAGGAA GATCACCCCT GCAAGCCGCT CGGAGAATAT GGGCTTTCCA AGCTGAAAAC CGAGGAACTG GCGGCGCAAT GGCGCACGCG CGGCATGAAT ATCTCACTCT TCCGCCCGCG CCTCATCATC GGCCCCGGAC GGCTTGGAAT TCTCGAAAAG CTGTTCAAGC TGATAGACAA TAACCTGCCC GTGCCGATGA TCGGCTCCGG CAAGAACCCC TATCAGTTTA TTTCGGTGTT CGATTGCGCA TCTGCCGCTC GCGCGGCCTT CAAGGCGGGC GTGCCGAACG AGGTGTATAA TCTCGGTTCG CTCAATCCGC CGCCGGTGCG CAAGCTTCTG GGCGATCTGA TCAAACATGC GGGTTCAAAA TCGATGCTGC TTCCCACGCC TGCCTGGGCG GTGAAGCGTA CGCTTGATTT CCTCGACTGG CTTAACATGC CGCTCATGGA CCCGGAGCAA TATCTGATCG CCGATGAGAT GTGCGTTCTC GACGTTTCCA AGGGCGAGCG TGAACTGGGC TGGATTCCGC AATATCGCGA TGAAGACATG CTGATCGCTG CCTATAGTGA ATATCGCAAG AAGAAGGCCG GCGGCGCTGA AAACCGCCTG AGCCCGTCAA AAGCGGCC

Details for BrsuB.00050.a.B1.GE37978

HARVESTED ON:	5/12/2014
SEQUENCED ON:	5/4/2014
EXPECTED MW:	39kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	89
PERCENT COVERAGE:	86

Validated AA Sequence

MXHEVIVXXX VKSDLAHYGD ATFVHTDVTD PESIRKLGIR ADDMVYNLSA KMLSPIQVRA KRHDFFFPVN YYGTENIIKA MDAAGAKKLV PFHHRYGLWP HLCLAAKRKI TPASRSENMG FSKLKTEELA AQWRTRGMNI SLFRPRLIIG PGRLGILEKL FKLIDNNLPV PMIGSGKNPY QFISVFDCAS AARAAFKAGV PNEVYNLGSL NPPPVRKLLG DLIKHAGSKS MLLPTPAWAV KRTLDFLDWL NMPLMDPEQY LIADEMCVLD VSKGERELGW IPQYRDEDML IAAYSEYRX

Validated NT Sequence

tnnnngcgat attcactata ggcagcgatc agcatgtctt catcgcgata ttgcggaatc cagcccagtt cacgctcgcc cttggaaacg tcgagaacgc acatctcatc ggcgatcaga tattgctccg ggtccatgag cggcatgtta agccagtcga ggaaatcaag cgtacgcttc accgcccagg caggcgtggg aagcagcatc gattttgaac ccgcatgttt gatcagatcg cccagaagct tgcgcaccgg cggcggattg agcgaaccga gattatacac ctcgttcggc acgcccgcct tgaaggccgc gcgagcggca gatgcgcaat cgaacaccga aataaactga taggggttct tgccggagcc gatcatcggc acgggcaggt tattgtctat cagcttgaac agcttttcga gaattccaag ccgtccgggg ccgatgatga ggcgcgggcg gaagagtgag atattcatgc cgcgcgtgcg ccattgcgcc gccagttcct cggttttcag cttggaaaag cccatattct ccgagcggct tgcaggggtg atcttccttt ttgcggccag acataggtgt ggccatagac catatcggtg gtgaaatggg accagctttt ttgcccccgc tgcatccatc gcctttatga tgttctctgt gccgtaatag ttcacgggga agaagaaatc atgccgcttg gcgcggacct gaatgggcga aagcatcttg gccgagagat tataaaccat gtcatcggcg cgaatgccaa gcttgcggat cgattccggg tccgtcacat ccgtatgaac gaaggtcgca tcgccataat gcgcaagatc gcttttgacg anntnnnnga cgatcacctc atggnncatc ggccagcant tngggcgnga gnnnncnccc gacaaaannn gtcaccgccg aaaataatat gcctcatatg gtggtggngg nggngnncca tgg

Expected Protein Sequence

MAHHHHHHMR HIIFGGDGFV GRYLAPQLLA DGHEVIVADI VKSDLAHYGD ATFVHTDVTD PESIRKLGIR ADDMVYNLSA KMLSPIQVRA KRHDFFFPVN YYGTENIIKA MDAAGAKKLV HFTTDMLYGH TYVWPQKEDH PCKPLGEYGL SKLKTEELAA QWRTRGMNIS LFRPRLIIGP GRLGILEKLF KLIDNNLPVP MIGSGKNPYQ FISVFDCASA ARAAFKAGVP NEVYNLGSLN PPPVRKLLGD LIKHAGSKSM LLPTPAWAVK RTLDFLDWLN MPLMDPEQYL IADEMCVLDV SKGERELGWI PQYRDEDMLI AAYSEYRKKK AGGAENRLSP SKAA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaggc atattatttt cggcggtgac ggttttgtcg ggcgttatct cgcgccccaa ttgctggccg atggccatga ggtgatcgtc gccgatatcg tcaaaagcga tcttgcgcat tatggcgatg cgaccttcgt tcatacggat gtgacggacc cggaatcgat ccgcaagctt ggcattcgcg ccgatgacat ggtttataat ctctcggcca agatgctttc gcccattcag gtccgcgcca agcggcatga tttcttcttc cccgtgaact attacggcac agagaacatc ataaaggcga tggatgcagc gggggcaaaa aagctggtcc atttcaccac cgatatgctc tatggccaca cctatgtctg gccgcaaaag gaagatcacc cctgcaagcc gctcggagaa tatgggcttt ccaagctgaa aaccgaggaa ctggcggcgc aatggcgcac gcgcggcatg aatatctcac tcttccgccc gcgcctcatc atcggccccg gacggcttgg aattctcgaa aagctgttca agctgataga caataacctg cccgtgccga tgatcggctc cggcaagaac ccctatcagt ttatttcggt gttcgattgc gcatctgccg ctcgcgcggc cttcaaggcg ggcgtgccga acgaggtgta taatctcggt tcgctcaatc cgccgccggt gcgcaagctt ctgggcgatc tgatcaaaca tgcgggttca aaatcgatgc tgcttcccac gcctgcctgg gcggtgaagc gtacgcttga tttcctcgac tggcttaaca tgccgctcat ggacccggag caatatctga tcgccgatga gatgtgcgtt ctcgacgttt ccaagggcga gcgtgaactg ggctggattc cgcaatatcg cgatgaagac atgctgatcg ctgcctatag tgaatatcgc aagaagaagg ccggcggcgc tgaaaaccgc ctgagcccgt caaaagcggc ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat