SSGCID: Target Page

BrsuB.00027.e

Isovaleryl-CoA dehydrogenase (EC 1.3.99.10)

CENTER ID:	BrsuB.00027.e
ORGANISM:	Brucella suis ATCC 23445
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuB.00027.e.B1.GE37102	Full length( BrsuB.00027.e )		1	382

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruSui83806_1542
BV-BRC:	fig\|470137.4.peg.1542
RefSeq:	YP_001626702.1
UniProt:	B0CI42

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNFGLGEEIE ALRDTVRRFA ESRIAPLAAE TDRNNQFPMH LWREFGELGV LGITAPEDYG GAGMGYLAHC IAMEEISRAS ASIGLSYGAH SNLCVNQITR NGSPEQRAKY LPKLISGEHV GALAMSEPGA GSDVVSMKLA AEKRGDRYVL NGNKMWITNG PDADVLVVYA KTDLSAGPRG ISAFIIEKGF KGFSTAQKLD KLGMRGSNTC ELVFEDCEVP AENLLGTEGK GVNVLMSGLD YERVVLPGGP LGIMAACLDV VVPYVHERKQ FDQPIGEFQL MQCKLADMYV TFNASRAYVY AVAAACDRGE TTRKDAAGCI LYSAEKATQM ALQAIQSLGG NGYINDYPTG RLLRDAKLYE IGAGTSEIRR MLIGRELFQE TR

NT Sequence

ATGAACTTCG GTCTTGGCGA GGAGATCGAA GCCCTTCGTG ACACTGTGCG CCGTTTCGCC GAAAGCCGCA TTGCGCCGCT TGCCGCTGAA ACCGACCGCA ATAATCAATT TCCCATGCAT CTTTGGCGTG AATTTGGTGA GCTTGGCGTA CTCGGCATTA CGGCGCCGGA AGACTATGGT GGCGCAGGCA TGGGCTATCT CGCCCATTGC ATCGCCATGG AGGAGATCAG CCGTGCTTCC GCCTCCATCG GCCTTAGCTA CGGCGCCCAT TCCAATCTCT GCGTCAACCA GATCACCCGC AACGGTTCGC CCGAACAGCG CGCGAAATAT CTGCCGAAAC TCATTTCCGG TGAGCATGTC GGCGCGCTGG CCATGTCTGA GCCGGGCGCG GGGTCCGATG TTGTTTCCAT GAAGCTTGCC GCCGAAAAGC GCGGGGACCG TTACGTCCTC AACGGCAACA AGATGTGGAT CACCAATGGC CCGGATGCCG ATGTGCTGGT GGTCTATGCC AAGACAGACC TGTCGGCTGG CCCGCGCGGG ATCAGCGCCT TTATCATTGA AAAAGGGTTC AAGGGTTTTT CGACCGCGCA AAAGCTCGAC AAACTCGGTA TGCGCGGTTC CAACACCTGC GAACTGGTGT TCGAGGATTG CGAAGTGCCA GCCGAAAACC TGCTCGGAAC CGAGGGCAAG GGCGTCAATG TGCTGATGTC CGGCCTCGAT TATGAGCGCG TGGTGCTGCC GGGTGGGCCG CTCGGCATCA TGGCGGCCTG CCTCGATGTC GTGGTGCCCT ATGTGCATGA GCGCAAGCAG TTCGACCAGC CAATCGGTGA ATTCCAGCTC ATGCAGTGCA AGCTTGCCGA CATGTATGTG ACCTTCAACG CTTCACGCGC TTATGTCTAT GCCGTGGCCG CAGCCTGCGA CCGGGGTGAG ACGACGCGCA AGGATGCGGC GGGCTGCATT CTTTACTCTG CGGAAAAAGC CACGCAGATG GCCTTGCAGG CGATCCAGTC TCTCGGCGGC AACGGCTATA TCAACGATTA TCCGACGGGC CGCCTTTTGC GCGATGCCAA GCTTTATGAA ATCGGCGCGG GCACGTCGGA AATCCGGCGT ATGCTGATCG GACGGGAACT CTTTCAGGAG ACCCGT

Details for BrsuB.00027.e.B1.GE37102

HARVESTED ON:	7/17/2013
SEQUENCED ON:	7/20/2013
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMN FGLGEEIEAL RDTVRRFAES RIAPLAAETD RNNQFPMHLW REFGELGVLG ITAPEDYGGA GMGYLAHCIA MEEISRASAS IGLSYGAHSN LCVNQITRNG SPEQRAKYLP KLISGEHVGA LAMSEPGAGS DVVSMKLAAE KRGDRYVLNG NKMWITNGPD ADVLVVYAKT DLSAGPRGIS AFIIEKGFKG FSTAQKLDKL GMRGSNTCEL VFEDCEVPAE NLLGTEGKGV NVLMSGLDYE RVVLPGGPLG IMAACLDVVV PYVHERKQFD QPIGEFQLMQ CKLADMYVTF NASRAYVYAV AAACDRGETT RKDAAGCILY SAEKATQMAL QAIQSLGGNG YINDYPTGRL LRDAKLYEIG AGTSEIRRML IGRELFQETR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaact tcggtcttgg cgaggagatc gaagcccttc gtgacactgt gcgccgtttc gccgaaagcc gcattgcgcc gcttgccgct gaaaccgacc gcaataatca atttcccatg catctttggc gtgaatttgg tgagcttggc gtactcggca ttacggcgcc ggaagactat ggtggcgcag gcatgggcta tctcgcccat tgcatcgcca tggaggagat cagccgtgct tccgcctcca tcggccttag ctacggcgcc cattccaatc tctgcgtcaa ccagatcacc cgcaacggtt cgcccgaaca gcgcgcgaaa tatctgccga aactcatttc cggtgagcat gtcggcgcgc tggccatgtc tgagccgggc gcggggtccg atgttgtttc catgaagctt gccgccgaaa agcgcgggga ccgttacgtc ctcaacggca acaagatgtg gatcaccaat ggcccggatg ccgatgtgct ggtggtctat gccaagacag acctgtcggc tggcccgcgc gggatcagcg cctttatcat tgaaaaaggg ttcaagggtt tttcgaccgc gcaaaagctc gacaaactcg gtatgcgcgg ttccaacacc tgcgaactgg tgttcgagga ttgcgaagtg ccagccgaaa acctgctcgg aaccgagggc aagggcgtca atgtgctgat gtccggcctc gattatgagc gcgtggtgct gccgggtggg ccgctcggca tcatggcggc ctgcctcgat gtcgtggtgc cctatgtgca tgagcgcaag cagttcgacc agccaatcgg tgaattccag ctcatgcagt gcaagcttgc cgacatgtat gtgaccttca acgcttcacg cgcttatgtc tatgccgtgg ccgcagcctg cgaccggggt gagacgacgc gcaaggatgc ggcgggctgc attctttact ctgcggaaaa agccacgcag atggccttgc aggcgatcca gtctctcggc ggcaacggct atatcaacga ttatccgacg ggccgccttt tgcgcgatgc caagctttat gaaatcggcg cgggcacgtc ggaaatccgg cgtatgctga tcggacggga actctttcag gagacccgtt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat