SSGCID: Target Page

BrsuB.00027.d

acyl-CoA dehydrogenase

CENTER ID:	BrsuB.00027.d
ORGANISM:	Brucella suis ATCC 23445
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuB.00027.d.B1.GE37101	Full length( BrsuB.00027.d )		1	257

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruSui83806_1149

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTEPDVGSDA GAVRTTAERD GDSYIINGTK RFITNAPVAG VFTLMARTGG SGPSGISAFL VERDLPGLTV GTPDHKMGQR GTQTCDVYLE NVRVPATSII GGVEGRGFKT AMKVLNRGRL HISSVCAGTA ERLIEESVGF AGSRVQFGKR IAEHQMIQAM LADMSTEAYA GRCMVLDAAR AFDESNVDII RKAASCKLFC SEMVGRVADN AVQIHGGSGY MQNYPVEHFY RDVRLFRLYE GTSQIQRIII AREMIRE

NT Sequence

TTGACAGAGC CTGATGTCGG TTCGGATGCC GGTGCAGTAC GCACCACTGC CGAACGCGAC GGAGACAGCT ATATCATCAA CGGCACGAAG CGCTTCATTA CCAATGCGCC GGTTGCCGGA GTTTTCACGC TCATGGCTCG TACGGGTGGT TCGGGCCCAT CGGGAATCAG CGCCTTTCTG GTGGAAAGGG ATTTGCCCGG TCTCACGGTC GGAACACCCG ACCACAAAAT GGGCCAGAGA GGCACCCAAA CCTGCGATGT CTATCTTGAG AATGTACGCG TTCCTGCTAC AAGCATAATT GGCGGGGTCG AAGGACGCGG TTTCAAGACC GCTATGAAAG TACTCAATCG CGGGCGTCTT CATATATCGT CCGTCTGCGC CGGTACGGCG GAGCGTCTGA TTGAGGAATC TGTCGGCTTT GCCGGCAGCC GCGTACAGTT TGGCAAACGT ATCGCCGAAC ATCAGATGAT TCAGGCGATG CTGGCCGATA TGAGCACAGA AGCCTATGCG GGGCGTTGCA TGGTTCTTGA TGCCGCACGG GCCTTTGATG AAAGCAACGT CGATATCATT CGCAAAGCTG CAAGCTGCAA GTTGTTTTGT TCCGAAATGG TTGGAAGGGT TGCAGACAAT GCCGTGCAGA TACACGGCGG TTCCGGATAT ATGCAGAACT ATCCGGTCGA GCACTTCTAT CGCGATGTAC GTTTGTTCCG CCTCTATGAG GGAACCTCGC AAATCCAGCG AATTATCATA GCGCGCGAAA TGATAAGGGA G

Details for BrsuB.00027.d.B1.GE37101

HARVESTED ON:	7/17/2013
SEQUENCED ON:	7/20/2013
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHHM LTEPDVGSDA GAVRTTAERD GDSYIINGTK RFITNAPVAG VFTLMARTGG SGPSGISAFL VERDLPGLTV GTPDHKMGQR GTQTCDVYLE NVRVPATSII GGVEGRGFKT AMKVLNRGRL HISSVCAGTA ERLIEESVGF AGSRVQFGKR IAEHQMIQAM LADMSTEAYA GRCMVLDAAR AFDESNVDII RKAASCKLFC SEMVGRVADN AVQIHGGSGY MQNYPVEHFY RDVRLFRLYE GTSQIQRIII AREMIRE

Validated NT Sequence

annnnttttn nttactttaa gaaggagana taccatggct caccaccacc accaccacca tatgttgaca gagcctgatg tcggttcgga tgccggtgca gtacgcacca ctgccgaacg cgacggagac agctatatca tcaacggcac gaagcgcttc attaccaatg cgccggttgc cggagttttc acgctcatgg ctcgtacggg tggttcgggc ccatcgggaa tcagcgcctt tctggtggaa agggatttgc ccggtctcac ggtcggaaca cccgaccaca aaatgggcca gagaggcacc caaacctgcg atgtctatct tgagaatgta cgcgttcctg ctacaagcat aattggcggg gtcgaaggac gcggtttcaa gaccgctatg aaagtactca atcgcgggcg tcttcatata tcgtccgtct gcgccggtac ggcggagcgt ctgattgagg aatctgtcgg ctttgccggc agccgcgtac agtttggcaa acgtatcgcc gaacatcaga tgattcaggc gatgctggcc gatatgagca cagaagccta tgcggggcgt tgcatggttc ttgatgccgc acgggccttt gatgaaagca acgtcgatat cattcgcaaa gctgcaagct gcaagttgtt ttgttccgaa atggttggaa gggttgcaga caatgccgtg cagatacacg gcggctccgg atatatgcag aactatccgg tcgagcactt ctatcgcgat gtacgtttgt tccgcctcta tgagggaacc tcgcaaatcc agcgaattat catagcgcgc gaaatgataa gggagtaagt gagtaagata ggatccggct gctacnnnnn ccnnaaag

Expected Protein Sequence

MAHHHHHHMT EPDVGSDAGA VRTTAERDGD SYIINGTKRF ITNAPVAGVF TLMARTGGSG PSGISAFLVE RDLPGLTVGT PDHKMGQRGT QTCDVYLENV RVPATSIIGG VEGRGFKTAM KVLNRGRLHI SSVCAGTAER LIEESVGFAG SRVQFGKRIA EHQMIQAMLA DMSTEAYAGR CMVLDAARAF DESNVDIIRK AASCKLFCSE MVGRVADNAV QIHGGSGYMQ NYPVEHFYRD VRLFRLYEGT SQIQRIIIAR EMIRE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgacag agcctgatgt cggttcggat gccggtgcag tacgcaccac tgccgaacgc gacggagaca gctatatcat caacggcacg aagcgcttca ttaccaatgc gccggttgcc ggagttttca cgctcatggc tcgtacgggt ggttcgggcc catcgggaat cagcgccttt ctggtggaaa gggatttgcc cggtctcacg gtcggaacac ccgaccacaa aatgggccag agaggcaccc aaacctgcga tgtctatctt gagaatgtac gcgttcctgc tacaagcata attggcgggg tcgaaggacg cggtttcaag accgctatga aagtactcaa tcgcgggcgt cttcatatat cgtccgtctg cgccggtacg gcggagcgtc tgattgagga atctgtcggc tttgccggca gccgcgtaca gtttggcaaa cgtatcgccg aacatcagat gattcaggcg atgctggccg atatgagcac agaagcctat gcggggcgtt gcatggttct tgatgccgca cgggcctttg atgaaagcaa cgtcgatatc attcgcaaag ctgcaagctg caagttgttt tgttccgaaa tggttggaag ggttgcagac aatgccgtgc agatacacgg cggttccgga tatatgcaga actatccggt cgagcacttc tatcgcgatg tacgtttgtt ccgcctctat gagggaacct cgcaaatcca gcgaattatc atagcgcgcg aaatgataag ggagtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat