SSGCID: Target Page

BrsuA.18828.a

BspF VirB type IV secretion effector protein

CENTER ID:	BrsuA.18828.a
ORGANISM:	Brucella suis 1330
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuA.18828.a.B1.GE38177	full length		1	428

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuA.18828.a.B1.PW37626	full length		1	428

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|204722.5.peg.1981
RefSeq:	NP_698924.1
RefSeq:	YP_005616748.1
UniProt:	A0A0H3G4R9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAAKPLLEES AGGNAAHLVS QLSDSSAVIE AAKKMEESLH SRDIPRKLAI YGAAAGFELR DELDHLSNRT IEPNIFFNAR FLAPAMPRLE NREVRFMVMR DENETRSRLR FVMPYTVERP GMPLSVPVIR SWATPFGPQG TPLIDHDNPV GVVEDLFDIL ARRHIKLPEV LVLPEMRANG AAARLIRSVA IGRQLPVQSI EHKERPFLES KLDGKTYIKE AIGAHHRHDY ERLWRRLAEQ GELVHKVART PDEVRHAFEH FLTLEARGWK GRRGTAMAVD RFRAAFAREA VNNLAECDCV RIHTLELDGR VIAILIVFTV SGEAWTWKTA YDETFHAWSP GVLLMIEVVK NHLDDPNIAR TDSCAVPDHP VMTRLFRERE TMETLVVGLH PGADRLVRQA ASQIHLYQRT RNLARIIRNR LRSFTEHK

NT Sequence

atggctgcaa aacctttgct tgaggaatca gcaggcggaa acgccgccca tcttgtttcg caactttccg acagcagcgc cgtgatcgaa gcggcgaaga agatggagga aagcctgcac agccgcgata ttccgcgcaa gcttgccatt tatggcgcag cagccggttt tgaattgcgt gatgagctgg accatctgag caatcgcacc atagagccga acatcttctt caacgcacgt tttctggcgc ctgccatgcc acgccttgaa aaccgtgaag ttcgcttcat ggtcatgcgc gacgaaaatg aaacccgaag ccgtctgcgc tttgtgatgc cctatacggt ggagcgcccc ggcatgccac tttcggttcc cgtgattcgc tcctgggcca cccccttcgg cccgcagggc acgccgctca tcgaccatga caacccggtc ggggtggttg aagacctgtt cgacattctg gcgcgcaggc acatcaagct gcccgaagta ctggttttgc cggaaatgcg tgccaatgga gcagccgcca ggctgatccg ctcggtcgcc atcgggcgcc aattgccggt tcaatccatc gagcacaagg aacgtccctt ccttgaaagc aagctcgatg gcaagaccta tataaaggaa gccatcgggg cgcatcatcg ccatgactat gaacgattgt ggcgccgcct tgccgagcag ggcgaactcg ttcacaaggt tgcccgcacg cccgatgagg tgcgccatgc ctttgaacat ttcctcacac tggaggcgcg cggctggaag ggacgtcgcg gcacagccat ggctgttgac cgttttcgtg ccgctttcgc gcgcgaagcc gtcaacaatc ttgccgaatg cgactgtgta cgcattcaca cgctggaact tgatggcagg gtcatcgcaa tcctcatcgt cttcacggta tcaggcgaag cctggacctg gaaaaccgcc tatgacgaga catttcatgc ctggtcgccc ggcgttctcc tgatgatcga agtggtgaag aaccacctgg acgaccccaa tatcgcccgc accgattcct gcgccgtgcc agaccatccc gtcatgacgc gcctcttccg tgaacgcgag actatggaaa cgcttgtcgt cggccttcac cccggcgcgg atcggctggt gcggcaggcg gcatcgcaga ttcacctcta tcagcgcacc cgcaatctcg cgcgcatcat ccgcaaccgg ctgcgcagtt tcaccgagca taaataa

Details for BrsuA.18828.a.B1.GE38177

HARVESTED ON:	6/11/2014
SEQUENCED ON:	8/5/2016
EXPECTED MW:	50kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	81
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMA AKPLLEESAG GNAAHLVSQL SDSSAVIEAA KKMEESLHSR DIPRKLAIYG AAAGFELRDE LDHLSNRTIE PNIFFNARFL APAMPRLENR EVRFMVMRDE NETRSRLRFV MPYTVERPGM PLSVPVIRSW ATPFGPQGTP LIDHDNPVGV VEDLFDILAR RHIKLPEVLV LPEMRANGAA ARLIRSVAIG RQLPVQSIEH KERPFLESKL DGKTYIKEAI GAHHRHDYER LWRRLAEQGE LVHKVARTPD EVRHAFEHFL TLEARGWKGR RGTAMAVDRF RAAFAREAVN NLAEXXCVRI HTLELDGXVI AILIXXXXSX XAWXXXXXXD ETFHAWXPGX XXMXEXXXXH XXDXXXXRXX SXXXPXHXXX XXXXXXXXXX EXXVVXXXPX XXXXXXQXXX XXHXXXXXAX XXASXX

Validated NT Sequence

atggctcacc accaccacca ccatatggct gcaaaacctt tgcttgagga atcagcaggc ggaaacgccg cccatcttgt ttcgcaactt tccgacagca gcgccgtgat cgaagcggcg aagaagatgg aggaaagcct gcacagccgc gatattccgc gcaagcttgc catttatggc gcagcagccg gttttgaatt gcgtgatgag ctggaccatc tgagcaatcg caccatagag ccgaacatct tcttcaacgc acgttttctg gcgcctgcca tgccacgcct tgaaaaccgt gaagttcgct tcatggtcat gcgcgacgaa aatgaaaccc gaagccgtct gcgctttgtg atgccctata cggtggagcg ccccggcatg ccactttcgg ttcccgtgat tcgctcctgg gccaccccct tcggcccgca gggcacgccg ctcatcgacc atgacaaccc ggtcggggtg gttgaagacc tgttcgacat tctggcgcgc aggcacatca agctgcccga agtactggtt ttgccggaaa tgcgtgccaa tggagcagcc gccaggctga tccgctcggt cgccatcggg cgccaattgc cggttcaatc catcgagcac aaggaacgtc ccttccttga aagcaagctc gatggcaaga cctatataaa ggaagccatc ggggcgcatc atcgccatga ctatgaacga ttgtggcgcc gccttgccga gcagggcgaa ctcgttcaca aggttgcccg cacgcccgat gaggtgcgcc atgcctttga acatttcctc acactggagg cgcgcggctg gaagggacgt cgcggcacag ccatggctgt tgaccgtttt cgtgccgctt tcgcgcgcga agccgtcaac aatcttgccg aatgnnactg tgtacgcatt cacacgctgg aacttgatgg cngggtcatc gcaatcctca tcgnnntcan ggnatcagnn naagcctggn cnngnaannc nncnnnngac gagacntttc atgcctggtn gccnggnntt ntccngatga tngaagngnn gnaganccac nnnnnngacn ccaanntngn ncgcnnnnnn tcnngnncng ngccngncca tnnnnncang nnnngcntnn nnnnnnaann nnngnnnang gaannnnnng tngtngncnt tcnnccnnnn nnnnntnngn ngnnnnngca gnnnncntng cngnntcacn nnatnagnnc nncngcaatn tngcncgcat catnnncnnc c

Expected Protein Sequence

MAHHHHHHMA AKPLLEESAG GNAAHLVSQL SDSSAVIEAA KKMEESLHSR DIPRKLAIYG AAAGFELRDE LDHLSNRTIE PNIFFNARFL APAMPRLENR EVRFMVMRDE NETRSRLRFV MPYTVERPGM PLSVPVIRSW ATPFGPQGTP LIDHDNPVGV VEDLFDILAR RHIKLPEVLV LPEMRANGAA ARLIRSVAIG RQLPVQSIEH KERPFLESKL DGKTYIKEAI GAHHRHDYER LWRRLAEQGE LVHKVARTPD EVRHAFEHFL TLEARGWKGR RGTAMAVDRF RAAFAREAVN NLAECDCVRI HTLELDGRVI AILIVFTVSG EAWTWKTAYD ETFHAWSPGV LLMIEVVKNH LDDPNIARTD SCAVPDHPVM TRLFRERETM ETLVVGLHPG ADRLVRQAAS QIHLYQRTRN LARIIRNRLR SFTEHK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggctg caaaaccttt gcttgaggaa tcagcaggcg gaaacgccgc ccatcttgtt tcgcaacttt ccgacagcag cgccgtgatc gaagcggcga agaagatgga ggaaagcctg cacagccgcg atattccgcg caagcttgcc atttatggcg cagcagccgg ttttgaattg cgtgatgagc tggaccatct gagcaatcgc accatagagc cgaacatctt cttcaacgca cgttttctgg cgcctgccat gccacgcctt gaaaaccgtg aagttcgctt catggtcatg cgcgacgaaa atgaaacccg aagccgtctg cgctttgtga tgccctatac ggtggagcgc cccggcatgc cactttcggt tcccgtgatt cgctcctggg ccaccccctt cggcccgcag ggcacgccgc tcatcgacca tgacaacccg gtcggggtgg ttgaagacct gttcgacatt ctggcgcgca ggcacatcaa gctgcccgaa gtactggttt tgccggaaat gcgtgccaat ggagcagccg ccaggctgat ccgctcggtc gccatcgggc gccaattgcc ggttcaatcc atcgagcaca aggaacgtcc cttccttgaa agcaagctcg atggcaagac ctatataaag gaagccatcg gggcgcatca tcgccatgac tatgaacgat tgtggcgccg ccttgccgag cagggcgaac tcgttcacaa ggttgcccgc acgcccgatg aggtgcgcca tgcctttgaa catttcctca cactggaggc gcgcggctgg aagggacgtc gcggcacagc catggctgtt gaccgttttc gtgccgcttt cgcgcgcgaa gccgtcaaca atcttgccga atgcgactgt gtacgcattc acacgctgga acttgatggc agggtcatcg caatcctcat cgtcttcacg gtatcaggcg aagcctggac ctggaaaacc gcctatgacg agacatttca tgcctggtcg cccggcgttc tcctgatgat cgaagtggtg aagaaccacc tggacgaccc caatatcgcc cgcaccgatt cctgcgccgt gccagaccat cccgtcatga cgcgcctctt ccgtgaacgc gagactatgg aaacgcttgt cgtcggcctt caccccggcg cggatcggct ggtgcggcag gcggcatcgc agattcacct ctatcagcgc acccgcaatc tcgcgcgcat catccgcaac cggctgcgca gtttcaccga gcataaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for BrsuA.18828.a.B1.PW37626

PURIFICATION DATe:	1/6/2015
CONCENTRATION:	5mg/ml
OBSERVED MW:	46kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	1
VIAL VOLUME:	200µl
PERCENT IDENTITY:	81
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMA AKPLLEESAG GNAAHLVSQL SDSSAVIEAA KKMEESLHSR DIPRKLAIYG AAAGFELRDE LDHLSNRTIE PNIFFNARFL APAMPRLENR EVRFMVMRDE NETRSRLRFV MPYTVERPGM PLSVPVIRSW ATPFGPQGTP LIDHDNPVGV VEDLFDILAR RHIKLPEVLV LPEMRANGAA ARLIRSVAIG RQLPVQSIEH KERPFLESKL DGKTYIKEAI GAHHRHDYER LWRRLAEQGE LVHKVARTPD EVRHAFEHFL TLEARGWKGR RGTAMAVDRF RAAFAREAVN NLAECDCVRI HTLELDGRVI AILIVFTVSG EAWTWKTAYD ETFHAWSPGV LLMIEVVKNH LDDPNIARTD SCAVPDHPVM TRLFRERETM ETLVVGLHPG ADRLVRQAAS QIHLYQRTRN LARIIRNRLR SFTEHK

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCTG CAAAACCTTT GCTTGAGGAA TCAGCAGGCG GAAACGCCGC CCATCTTGTT TCGCAACTTT CCGACAGCAG CGCCGTGATC GAAGCGGCGA AGAAGATGGA GGAAAGCCTG CACAGCCGCG ATATTCCGCG CAAGCTTGCC ATTTATGGCG CAGCAGCCGG TTTTGAATTG CGTGATGAGC TGGACCATCT GAGCAATCGC ACCATAGAGC CGAACATCTT CTTCAACGCA CGTTTTCTGG CGCCTGCCAT GCCACGCCTT GAAAACCGTG AAGTTCGCTT CATGGTCATG CGCGACGAAA ATGAAACCCG AAGCCGTCTG CGCTTTGTGA TGCCCTATAC GGTGGAGCGC CCCGGCATGC CACTTTCGGT TCCCGTGATT CGCTCCTGGG CCACCCCCTT CGGCCCGCAG GGCACGCCGC TCATCGACCA TGACAACCCG GTCGGGGTGG TTGAAGACCT GTTCGACATT CTGGCGCGCA GGCACATCAA GCTGCCCGAA GTACTGGTTT TGCCGGAAAT GCGTGCCAAT GGAGCAGCCG CCAGGCTGAT CCGCTCGGTC GCCATCGGGC GCCAATTGCC GGTTCAATCC ATCGAGCACA AGGAACGTCC CTTCCTTGAA AGCAAGCTCG ATGGCAAGAC CTATATAAAG GAAGCCATCG GGGCGCATCA TCGCCATGAC TATGAACGAT TGTGGCGCCG CCTTGCCGAG CAGGGCGAAC TCGTTCACAA GGTTGCCCGC ACGCCCGATG AGGTGCGCCA TGCCTTTGAA CATTTCCTCA CACTGGAGGC GCGCGGCTGG AAGGGACGTC GCGGCACAGC CATGGCTGTT GACCGTTTTC GTGCCGCTTT CGCGCGCGAA GCCGTCAACA ATCTTGCCGA ATGCGACTGT GTACGCATTC ACACGCTGGA ACTTGATGGC AGGGTCATCG CAATCCTCAT CGTCTTCACG GTATCAGGCG AAGCCTGGAC CTGGAAAACC GCCTATGACG AGACATTTCA TGCCTGGTCG CCCGGCGTTC TCCTGATGAT CGAAGTGGTG AAGAACCACC TGGACGACCC CAATATCGCC CGCACCGATT CCTGCGCCGT GCCAGACCAT CCCGTCATGA CGCGCCTCTT CCGTGAACGC GAGACTATGG AAACGCTTGT CGTCGGCCTT CACCCCGGCG CGGATCGGCT GGTGCGGCAG GCGGCATCGC AGATTCACCT CTATCAGCGC ACCCGCAATC TCGCGCGCAT CATCCGCAAC CGGCTGCGCA GTTTCACCGA GCATAAATGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT