SSGCID: Target Page

BrsuA.18826.a

BspH VirB type IV secretion effector protein

CENTER ID:	BrsuA.18826.a
ORGANISM:	Brucella suis 1330
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuA.18826.a.B1.GE38175	full length		1	479

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|204722.5.peg.1890
RefSeq:	NP_698836.1
RefSeq:	YP_005616660.1
UniProt:	A0A0H3G5E1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MASKTTLNAK NLEALGAKRL AELLIEISTG NANHKRLLRM ELVGNSSGAE LAREVRKRLG SIGRSKSWID WQKAKSVRAD LEAQRKSIAE KIAPTDPNEA FELIWQFLSL ADPIFERSCD GSGALIESFH AACEDAGRIA AMAKVPIDRL VEKIFSVLQD NGYGQYDALI EAMAPALGEA GLRKLQRAFE AWAKEPQPKV ASKDKEVIAW SSDGPVYRDQ LYASGKSITV KVVLQQVADA LGDVDLYIEQ QSKESQTFAT VTTDIARRLL AAGRAEDALT ALEKTKFDSC REIPFEWQIL RAEVLESLGR AEEAQQFRWK SFEQNLNDEL LREYVKRLPD FDDVEAEEKA FAFVRAVADA NHALYFFLQY PSLSEASKLV VSRASEMDGD HYELMSDAAE KLSAKFPLAA TILLRAMIDF TLNNARSSRY KHAARHLLEC GALSRSIEDY HSFAPHEAYI ADLRKMHGRK HGFWPLVDR

NT Sequence

atggcatcaa agactacctt gaatgcgaag aaccttgaag cacttggcgc taagcgcctt gctgagctat tgattgagat cagcaccggg aatgcgaacc ataagcgctt gcttcgtatg gaacttgtcg gaaattcgag tggtgccgag ttggcaaggg aagttcgtaa gcgtttgggc agtatcggac gttcaaaaag ctggattgac tggcagaagg caaagtccgt tagggctgat ctggaagccc agcgcaaatc tattgccgag aaaattgcgc ccactgatcc gaatgaggcg tttgaactga tctggcagtt tctatcgctc gcagacccga tttttgagcg ttcatgcgat ggaagtggcg cgttgatcga aagcttccat gccgcgtgcg aagatgctgg tcgcattgct gcaatggcaa aagtccccat tgaccgcttg gtcgagaaaa tcttctcggt cttgcaggac aatggatatg gtcaatatga tgcgctgatt gaggcaatgg ctccggctct cggtgaggcc ggcttgcgaa agcttcaacg agccttcgaa gcatgggcta aagagcccca gccaaaagtc gcaagcaaag acaaagaggt tattgcttgg agtagcgatg gccctgttta ccgcgatcag ctttatgcca gcggtaagtc gatcacggtc aaagttgtgc ttcagcaggt cgccgatgct cttggcgatg ttgatctcta tattgaacag caatctaaag aatcacagac gttcgctaca gtcaccacag atatcgctcg gaggctttta gccgctggtc gcgcggaaga cgcattaacc gctctggaga aaacaaaatt cgatagctgt cgggaaatcc cttttgaatg gcaaattttg cgtgctgagg tgctggaatc tctcggacgt gcagaagagg cgcaacagtt tcggtggaag agtttcgagc agaatctcaa tgatgagttg ctacgggaat atgtgaagcg acttcccgat tttgatgacg ttgaagctga agaaaaggca tttgcatttg tccgagccgt cgccgatgca aaccatgcac tctatttctt ccttcaatat ccatcactta gtgaagcgtc taagctggtg gtcagtcgag caagtgagat ggatggcgat cattatgagc tgatgtctga tgccgcagag aagctatcgg ccaaattccc tttggccgcg actattctgt tacgcgcaat gatagatttc acgctcaata acgctcgctc aagtcgttac aagcacgcag cacggcattt gcttgagtgt ggtgctctgt cccgtagcat cgaagattat cattccttcg cgccgcatga ggcttatatt gctgatctcc gaaagatgca cgggaggaaa catggcttct ggccgcttgt agatcggtga

Details for BrsuA.18826.a.B1.GE38175

HARVESTED ON:	6/11/2014
SEQUENCED ON:	2/22/2016
EXPECTED MW:	55kDa
OBSERVED MW:	55kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	97
PERCENT COVERAGE:	66

Validated AA Sequence

Validated NT Sequence

atggctcacc accaccacca ccatatggca tcaaagacta ccttgaatgc gaagaacctt gaagcacttg gcgctaagcg ccttgctgag ctattgattg agatcagcac cgggaatgcg aaccataagc gcttgcttcg tatggaactt gtcggaaatt cgagtggtgc cgagttggca agggaagttc gtaagcgttt gggcagtatc ggacgttcaa aaagctggat tgactggcag aaggcaaagt ccgttagggc tgatctggaa gcccagcgca aatctattgc cgagaaaatt gcgcccactg atccgaatga ggcgtttgaa ctgatctggc agtttctatc gctcgcagac ccgatttttg agcgttcatg cgatggaagt ggcgcgttga tcgaaagctt ccatgccgcg tgcgaagatg ctggtcgcat tgctgcaatg gcaaaagtcc ccattgaccg cttggtcgag aaaatcttct cggtcttgca ggacaatgga tatggtcaat atgatgcgct gattgaggca atggctccgg ctctcggtga ggccggcttg cgaaagcttc aacgagcctt cgaagcatgg gctaaagagc cccagccaaa agtcgcaagc aaagacaaag aggttattgc ttggagtagc gatggccctg tttaccgcga tcagctttat gccagcggta agtcgatcac ggtcaaagtt gtgcttcagc aggtcgccga tgctcttggc gatgttgatc tctatattga acagcaatct aaagaatcac agacgttcgc tacagtcacc acagatatcg ctcggaggct tttagccgct ggtcgcgcgg aagacgcatt aaccgctctg ganaaaacaa aattcgatag ctgtcgggaa atcccttttg aatggcaaat tttgcgtgct gaggtgctgg aatctctcgg acgtgcannn nnnnnnncaa cagttt

Expected Protein Sequence

MAHHHHHHMA SKTTLNAKNL EALGAKRLAE LLIEISTGNA NHKRLLRMEL VGNSSGAELA REVRKRLGSI GRSKSWIDWQ KAKSVRADLE AQRKSIAEKI APTDPNEAFE LIWQFLSLAD PIFERSCDGS GALIESFHAA CEDAGRIAAM AKVPIDRLVE KIFSVLQDNG YGQYDALIEA MAPALGEAGL RKLQRAFEAW AKEPQPKVAS KDKEVIAWSS DGPVYRDQLY ASGKSITVKV VLQQVADALG DVDLYIEQQS KESQTFATVT TDIARRLLAA GRAEDALTAL EKTKFDSCRE IPFEWQILRA EVLESLGRAE EAQQFRWKSF EQNLNDELLR EYVKRLPDFD DVEAEEKAFA FVRAVADANH ALYFFLQYPS LSEASKLVVS RASEMDGDHY ELMSDAAEKL SAKFPLAATI LLRAMIDFTL NNARSSRYKH AARHLLECGA LSRSIEDYHS FAPHEAYIAD LRKMHGRKHG FWPLVDR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcat caaagactac cttgaatgcg aagaaccttg aagcacttgg cgctaagcgc cttgctgagc tattgattga gatcagcacc gggaatgcga accataagcg cttgcttcgt atggaacttg tcggaaattc gagtggtgcc gagttggcaa gggaagttcg taagcgtttg ggcagtatcg gacgttcaaa aagctggatt gactggcaga aggcaaagtc cgttagggct gatctggaag cccagcgcaa atctattgcc gagaaaattg cgcccactga tccgaatgag gcgtttgaac tgatctggca gtttctatcg ctcgcagacc cgatttttga gcgttcatgc gatggaagtg gcgcgttgat cgaaagcttc catgccgcgt gcgaagatgc tggtcgcatt gctgcaatgg caaaagtccc cattgaccgc ttggtcgaga aaatcttctc ggtcttgcag gacaatggat atggtcaata tgatgcgctg attgaggcaa tggctccggc tctcggtgag gccggcttgc gaaagcttca acgagccttc gaagcatggg ctaaagagcc ccagccaaaa gtcgcaagca aagacaaaga ggttattgct tggagtagcg atggccctgt ttaccgcgat cagctttatg ccagcggtaa gtcgatcacg gtcaaagttg tgcttcagca ggtcgccgat gctcttggcg atgttgatct ctatattgaa cagcaatcta aagaatcaca gacgttcgct acagtcacca cagatatcgc tcggaggctt ttagccgctg gtcgcgcgga agacgcatta accgctctgg agaaaacaaa attcgatagc tgtcgggaaa tcccttttga atggcaaatt ttgcgtgctg aggtgctgga atctctcgga cgtgcagaag aggcgcaaca gtttcggtgg aagagtttcg agcagaatct caatgatgag ttgctacggg aatatgtgaa gcgacttccc gattttgatg acgttgaagc tgaagaaaag gcatttgcat ttgtccgagc cgtcgccgat gcaaaccatg cactctattt cttccttcaa tatccatcac ttagtgaagc gtctaagctg gtggtcagtc gagcaagtga gatggatggc gatcattatg agctgatgtc tgatgccgca gagaagctat cggccaaatt ccctttggcc gcgactattc tgttacgcgc aatgatagat ttcacgctca ataacgctcg ctcaagtcgt tacaagcacg cagcacggca tttgcttgag tgtggtgctc tgtcccgtag catcgaagat tatcattcct tcgcgccgca tgaggcttat attgctgatc tccgaaagat gcacgggagg aaacatggct tctggccgct tgtagatcgg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat