BrsuA.18694.a

Efflux pump membrane transporter BepE

CENTER ID: BrsuA.18694.a
ORGANISM: Brucella suis 1330
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuA.18694.a.B1.GE37610 Full length(BrsuA.18694.a) 1 1051
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|204722.5.peg.305
RefSeq: NP_697326.1
RefSeq: YP_005615146.1
UniProt: Q8G2M6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNRFFVDRPV FAAVISIVLV LAGLICIRIL PVAQYPELTP PQVVVSATYP GASAETVAQT VAAPLEQQIN GVENMLYMQS SSLGSGTMQL TVTFALGTDP DQATINVNNR VQRATSSLPQ EVQRLGVTVD KRFTTILGMV AMFATTDRYD RTYVGNYALL NVVDDLKRLP GVGDVQLLGN IDYSMRVWLR PDKLAQYNLT PSDVSAAIQE QNAQFAAGRF GDQPDPHAGP FTYTATTQGR LPDAAAFENI ILRSSSQNAA TLRLKDVARV ELGTESYLVD SNLNGTPAVP IAIYLQPGAN ALNTMELIQN RMNELKASFP AGIDYAIPFD TTKFIKVSIE EVVHTFIEAI ILVVLVVFIF LQNWRATLIP VIAVPISIIG TFAGMYVLGF SINLLTLFGL VLAIGIVVDD AIVVLENVER IMTTEKLSPR KAAIKAMGEV TGPVIAIVLV LCAVFIPVAF MGGLVGEMYK QFAVTIAISV TLSGLVALTL TPALCALILK PGHHEPILPF RIFNRAFERV TSGYTRGVRF FLKRATIGLI IFAGLLGSTY YLFERVPGSL LPDEDQGFLF GVAVLPPAAS LERTTVVLDQ VSENIRKNPA VDNVFAVSGF DLLSGGLKTS AGTMFIMLKD WKERTTPDAD ARNLPRTIMG MNAGIKDGMV LAFNPPPIMG LSTTGGFELY VQDRTGGGVE SLTQATKLIT EAAAKRPELQ GVRTTFDPNV PQYDIQLDRE KAKAMGVPIN SVFTAMQATF GSLYVNDFTL YGRNYQVNLQ SEAEFRRDPG DLKHVFVRAD SGSMIPLDAL VTVKRIVGPD QLERFNAFNA AKVTGNPAPG YTSGDAIKAM QEVAAQVLPQ GYQIAWTGSA YQEVSTSGTG SQAMIFGLIM VFLILAAQYE RWSLPLAVIT AVPFAIFGAL LATDLRGLTN DVYFQIGLVT LIGLAAKNAI LIVEFAVLER ESGKSAIEAA ASAARLRFRP IVMTSLAFIL GVVPLAVSTG AGSASRHSIG TGVIGGMLAA TFIATFFIPM FYSLIARKPP KKHEDETADT PETPGGTGGG I
NT Sequence
ATGAACAGGT TCTTTGTCGA TCGCCCGGTT TTCGCGGCGG TCATATCCAT CGTTCTCGTG CTGGCTGGCC TCATCTGCAT CCGCATTCTG CCAGTTGCCC AATATCCCGA GCTGACGCCG CCGCAGGTGG TTGTGAGCGC GACCTATCCG GGTGCAAGCG CGGAAACCGT GGCGCAGACC GTTGCAGCGC CGCTGGAACA GCAGATCAAC GGCGTCGAGA ACATGCTTTA TATGCAGTCC TCGAGCCTTG GTTCGGGCAC TATGCAATTG ACCGTGACCT TCGCACTGGG CACCGACCCG GACCAGGCCA CGATCAACGT GAACAACCGC GTTCAACGCG CCACCTCCTC GCTGCCGCAG GAAGTGCAGC GCCTTGGCGT GACCGTGGAC AAGCGTTTCA CGACCATTCT CGGCATGGTC GCGATGTTCG CCACCACCGA TCGTTACGAC CGCACCTATG TCGGCAATTA TGCGCTTTTG AACGTCGTGG ACGATCTCAA GCGCCTGCCG GGCGTAGGTG ACGTGCAGTT GCTCGGCAAT ATCGATTATT CGATGCGCGT CTGGCTGCGC CCGGACAAGC TTGCCCAGTA CAATCTGACA CCGAGCGATG TTTCCGCAGC CATTCAGGAA CAGAACGCGC AGTTCGCCGC TGGCCGTTTC GGCGATCAGC CCGATCCGCA CGCAGGGCCC TTCACCTATA CGGCCACCAC ACAGGGCCGT TTGCCGGATG CCGCCGCTTT CGAGAATATC ATCCTGCGCT CATCGAGCCA GAATGCCGCG ACGCTTCGTC TGAAGGACGT GGCCCGTGTC GAGCTTGGCA CGGAAAGCTA TCTGGTTGAT AGTAACCTGA ACGGTACGCC CGCCGTTCCG ATCGCGATCT ATCTCCAGCC GGGCGCGAAT GCGCTCAACA CAATGGAGCT GATCCAGAAC CGGATGAACG AGCTGAAGGC AAGCTTTCCC GCCGGTATCG ACTATGCGAT CCCCTTCGAT ACGACCAAGT TCATCAAGGT CTCCATCGAG GAAGTGGTTC ACACCTTCAT CGAGGCTATC ATCCTCGTCG TGCTGGTGGT GTTCATCTTC CTGCAGAACT GGCGTGCAAC ACTCATTCCC GTCATCGCGG TGCCGATCTC GATCATCGGT ACATTTGCGG GCATGTATGT GCTTGGCTTC TCGATCAATC TTCTCACATT GTTCGGGCTT GTGCTCGCCA TCGGTATCGT GGTCGACGAC GCCATCGTGG TGCTGGAAAA TGTCGAACGC ATCATGACCA CCGAAAAACT CTCGCCACGC AAGGCCGCCA TCAAGGCCAT GGGCGAGGTG ACGGGGCCGG TGATCGCTAT CGTTCTCGTG CTTTGCGCCG TGTTCATTCC GGTTGCGTTC ATGGGCGGTC TGGTTGGCGA AATGTACAAG CAGTTCGCCG TCACCATCGC CATTTCTGTG ACGCTCTCCG GCCTTGTGGC CCTGACGCTG ACGCCCGCAC TGTGCGCGCT CATCCTCAAG CCCGGCCATC ATGAGCCGAT CCTGCCGTTC CGCATTTTCA ACCGTGCCTT CGAGCGCGTG ACGAGCGGTT ATACGCGCGG TGTCCGCTTC TTCCTGAAGC GCGCCACCAT CGGCCTCATC ATCTTTGCGG GCCTGCTCGG CAGCACTTAT TACCTGTTCG AGCGGGTTCC CGGTTCGCTA TTGCCGGATG AAGACCAGGG CTTCCTGTTC GGTGTCGCCG TTCTGCCCCC AGCCGCTTCG CTGGAGCGCA CGACTGTCGT GCTCGACCAG GTGAGCGAGA ACATCCGCAA GAACCCTGCC GTGGACAATG TCTTCGCCGT CTCTGGCTTC GACCTTCTGT CGGGCGGTTT GAAGACCAGT GCAGGCACCA TGTTCATCAT GCTCAAGGAC TGGAAGGAGC GCACCACGCC CGACGCGGAT GCGCGCAACC TGCCACGCAC CATCATGGGC ATGAATGCCG GTATCAAGGA CGGCATGGTT CTGGCCTTCA ACCCGCCTCC GATCATGGGT CTCAGCACGA CGGGCGGCTT TGAACTCTAT GTTCAGGACC GCACGGGCGG CGGTGTGGAA TCGCTGACGC AGGCAACGAA GCTCATCACT GAGGCGGCTG CCAAGCGCCC CGAATTGCAG GGCGTGCGCA CAACCTTCGA CCCGAATGTC CCCCAATATG ACATTCAGCT CGACCGCGAG AAGGCCAAGG CGATGGGTGT GCCGATCAAT TCGGTGTTCA CCGCCATGCA GGCAACCTTT GGCAGCCTCT ATGTGAATGA CTTCACGCTC TATGGCCGCA ACTATCAGGT TAACCTGCAA TCGGAAGCGG AATTCCGCCG CGATCCGGGC GATCTCAAGC ATGTTTTCGT GCGCGCCGAT TCCGGCAGCA TGATCCCTCT TGATGCGCTG GTGACGGTGA AGCGTATTGT CGGTCCCGAC CAGCTTGAAC GTTTCAATGC GTTCAACGCC GCAAAGGTCA CCGGCAACCC GGCTCCGGGC TATACGTCGG GTGATGCGAT CAAGGCCATG CAGGAAGTGG CGGCGCAAGT TCTGCCGCAG GGCTACCAGA TCGCATGGAC CGGCTCGGCC TATCAGGAAG TCTCCACCAG CGGCACCGGC TCGCAGGCGA TGATCTTCGG CCTTATCATG GTGTTCCTGA TTCTCGCTGC GCAATATGAA CGCTGGAGCC TGCCGCTTGC GGTTATCACC GCCGTGCCTT TCGCCATCTT CGGTGCGCTT CTGGCGACCG ATCTGCGCGG GCTTACCAAT GACGTCTATT TCCAGATCGG TCTGGTGACG CTCATCGGTC TTGCGGCGAA AAACGCGATC CTGATCGTGG AGTTCGCGGT GCTGGAGCGT GAGTCGGGTA AATCCGCAAT CGAGGCGGCG GCATCGGCGG CGCGCCTGCG CTTCCGCCCG ATCGTGATGA CGTCGCTTGC CTTCATCCTG GGCGTGGTGC CGCTTGCGGT CAGCACCGGG GCTGGCTCGG CCAGCCGTCA TTCCATCGGT ACGGGCGTGA TCGGCGGTAT GCTGGCGGCG ACCTTCATCG CAACCTTCTT CATCCCGATG TTCTACAGCC TGATCGCGCG CAAGCCGCCG AAGAAGCATG AGGATGAGAC AGCCGATACA CCCGAAACGC CGGGCGGTAC GGGCGGCGGT ATT
Details for BrsuA.18694.a.B1.GE37610
HARVESTED ON: 1/13/2014
SEQUENCED ON: 1/9/2014
EXPECTED MW: 114kDa
OBSERVED MW: 115kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 30
Validated AA Sequence
MAHHHHHHMN RFFVDRPVFA AVISIVLVLA GLICIRILPV AQYPELTPPQ VVVSATYPGA SAETVAQTVA APLEQQINGV ENMLYMQSSS LGSGTMQLTV TFALGTDPDQ ATINVNNRVQ RATSSLPQEV QRLGVTVDKR FTTILGMVAM FATTDRYDRT YVGNYALLNV VDDLKRLPGV GDVQLLGNID YSMRVWLRPD KLAQYNLTPS DVSAAIQEQN AQFAAGRFGD QPDPHAGPFT YTATTQGRLP DAAAFENIIL RSSSQNAATL RLKDVARVEL GTESYLVDSN LNGTPAVPIA IYLQPGANAL NTMELIQNRM TS
Validated NT Sequence
tnnnctannt attttgttta actttaagaa ggagatatac catggctcac caccaccacc accatatgaa caggttcttt gtcgatcgcc cggttttcgc ggcggtcata tccatcgttc tcgtgctggc tggcctcatc tgcatccgca ttctgccagt tgcccaatat cccgagctga cgccgccgca ggtggttgtg agcgcgacct atccgggtgc aagcgcggaa accgtggcgc agaccgttgc agcgccgctg gaacagcaga tcaacggcgt cgagaacatg ctttatatgc agtcctcgag ccttggttcg ggcactatgc aattgaccgt gaccttcgca ctgggcaccg acccggacca ggccacgatc aacgtgaaca accgcgttca acgcgccacc tcctcgctgc cgcaggaagt gcagcgcctt ggcgtgaccg tggacaagcg tttcacgacc attctcggca tggtcgcgat gttcgccacc accgatcgtt acgaccgcac ctatgtcggc aattatgcgc ttttgaacgt cgtggacgat ctcaagcgcc tgccgggcgt aggtgacgtg cagttgctcg gcaatatcga ttattcgatg cgcgtctggc tgcgcccgga caagcttgcc cagtacaatc tgacaccgag cgatgtttcc gcagccattc aggaacagaa cgcgcagttc gccgctggcc gtttcggcga tcagcccgat ccgcacgcag ggcccttcac ctatacggcc accacacagg gccgtttgcc ggatgccgcc gctttcgaga atatcatcct gcgctcatcg agccagaatg ccgcgacgct tcgtctgaag gacgtggccc gtgtcgagct tggcacggaa agctatctgg ttgatagtaa cctgaacggt acgcccgccg ttccgatcgc gatctatctc cagccgggcg cgaatgcgct caacacaatg gagctgatcc agaaccggat gacgagctga aggcaagctt tcccgccggt atcgactatg cgatcccctt cgatacgacc agttcatcnn nctcatcgag nnnngtcaca ncttcatcga ggctatcatc tcgncnngct ggtggnntca tntcngcnna nctggcnngc aanactcatn ccgtcatcgc ggnnncgaat ntngatcatc gnncnntnnn ggnnnnnnnn nnnnnnnnnn nnnnntcnnn nnnnnnnngg gnnnnnnnnn gccnnactnn nnnntncgng g
Expected Protein Sequence
MAHHHHHHMN RFFVDRPVFA AVISIVLVLA GLICIRILPV AQYPELTPPQ VVVSATYPGA SAETVAQTVA APLEQQINGV ENMLYMQSSS LGSGTMQLTV TFALGTDPDQ ATINVNNRVQ RATSSLPQEV QRLGVTVDKR FTTILGMVAM FATTDRYDRT YVGNYALLNV VDDLKRLPGV GDVQLLGNID YSMRVWLRPD KLAQYNLTPS DVSAAIQEQN AQFAAGRFGD QPDPHAGPFT YTATTQGRLP DAAAFENIIL RSSSQNAATL RLKDVARVEL GTESYLVDSN LNGTPAVPIA IYLQPGANAL NTMELIQNRM NELKASFPAG IDYAIPFDTT KFIKVSIEEV VHTFIEAIIL VVLVVFIFLQ NWRATLIPVI AVPISIIGTF AGMYVLGFSI NLLTLFGLVL AIGIVVDDAI VVLENVERIM TTEKLSPRKA AIKAMGEVTG PVIAIVLVLC AVFIPVAFMG GLVGEMYKQF AVTIAISVTL SGLVALTLTP ALCALILKPG HHEPILPFRI FNRAFERVTS GYTRGVRFFL KRATIGLIIF AGLLGSTYYL FERVPGSLLP DEDQGFLFGV AVLPPAASLE RTTVVLDQVS ENIRKNPAVD NVFAVSGFDL LSGGLKTSAG TMFIMLKDWK ERTTPDADAR NLPRTIMGMN AGIKDGMVLA FNPPPIMGLS TTGGFELYVQ DRTGGGVESL TQATKLITEA AAKRPELQGV RTTFDPNVPQ YDIQLDREKA KAMGVPINSV FTAMQATFGS LYVNDFTLYG RNYQVNLQSE AEFRRDPGDL KHVFVRADSG SMIPLDALVT VKRIVGPDQL ERFNAFNAAK VTGNPAPGYT SGDAIKAMQE VAAQVLPQGY QIAWTGSAYQ EVSTSGTGSQ AMIFGLIMVF LILAAQYERW SLPLAVITAV PFAIFGALLA TDLRGLTNDV YFQIGLVTLI GLAAKNAILI VEFAVLERES GKSAIEAAAS AARLRFRPIV MTSLAFILGV VPLAVSTGAG SASRHSIGTG VIGGMLAATF IATFFIPMFY SLIARKPPKK HEDETADTPE TPGGTGGGI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaca ggttctttgt cgatcgcccg gttttcgcgg cggtcatatc catcgttctc gtgctggctg gcctcatctg catccgcatt ctgccagttg cccaatatcc cgagctgacg ccgccgcagg tggttgtgag cgcgacctat ccgggtgcaa gcgcggaaac cgtggcgcag accgttgcag cgccgctgga acagcagatc aacggcgtcg agaacatgct ttatatgcag tcctcgagcc ttggttcggg cactatgcaa ttgaccgtga ccttcgcact gggcaccgac ccggaccagg ccacgatcaa cgtgaacaac cgcgttcaac gcgccacctc ctcgctgccg caggaagtgc agcgccttgg cgtgaccgtg gacaagcgtt tcacgaccat tctcggcatg gtcgcgatgt tcgccaccac cgatcgttac gaccgcacct atgtcggcaa ttatgcgctt ttgaacgtcg tggacgatct caagcgcctg ccgggcgtag gtgacgtgca gttgctcggc aatatcgatt attcgatgcg cgtctggctg cgcccggaca agcttgccca gtacaatctg acaccgagcg atgtttccgc agccattcag gaacagaacg cgcagttcgc cgctggccgt ttcggcgatc agcccgatcc gcacgcaggg cccttcacct atacggccac cacacagggc cgtttgccgg atgccgccgc tttcgagaat atcatcctgc gctcatcgag ccagaatgcc gcgacgcttc gtctgaagga cgtggcccgt gtcgagcttg gcacggaaag ctatctggtt gatagtaacc tgaacggtac gcccgccgtt ccgatcgcga tctatctcca gccgggcgcg aatgcgctca acacaatgga gctgatccag aaccggatga acgagctgaa ggcaagcttt cccgccggta tcgactatgc gatccccttc gatacgacca agttcatcaa ggtctccatc gaggaagtgg ttcacacctt catcgaggct atcatcctcg tcgtgctggt ggtgttcatc ttcctgcaga actggcgtgc aacactcatt cccgtcatcg cggtgccgat ctcgatcatc ggtacatttg cgggcatgta tgtgcttggc ttctcgatca atcttctcac attgttcggg cttgtgctcg ccatcggtat cgtggtcgac gacgccatcg tggtgctgga aaatgtcgaa cgcatcatga ccaccgaaaa actctcgcca cgcaaggccg ccatcaaggc catgggcgag gtgacggggc cggtgatcgc tatcgttctc gtgctttgcg ccgtgttcat tccggttgcg ttcatgggcg gtctggttgg cgaaatgtac aagcagttcg ccgtcaccat cgccatttct gtgacgctct ccggccttgt ggccctgacg ctgacgcccg cactgtgcgc gctcatcctc aagcccggcc atcatgagcc gatcctgccg ttccgcattt tcaaccgtgc cttcgagcgc gtgacgagcg gttatacgcg cggtgtccgc ttcttcctga agcgcgccac catcggcctc atcatctttg cgggcctgct cggcagcact tattacctgt tcgagcgggt tcccggttcg ctattgccgg atgaagacca gggcttcctg ttcggtgtcg ccgttctgcc cccagccgct tcgctggagc gcacgactgt cgtgctcgac caggtgagcg agaacatccg caagaaccct gccgtggaca atgtcttcgc cgtctctggc ttcgaccttc tgtcgggcgg tttgaagacc agtgcaggca ccatgttcat catgctcaag gactggaagg agcgcaccac gcccgacgcg gatgcgcgca acctgccacg caccatcatg ggcatgaatg ccggtatcaa ggacggcatg gttctggcct tcaacccgcc tccgatcatg ggtctcagca cgacgggcgg ctttgaactc tatgttcagg accgcacggg cggcggtgtg gaatcgctga cgcaggcaac gaagctcatc actgaggcgg ctgccaagcg ccccgaattg cagggcgtgc gcacaacctt cgacccgaat gtcccccaat atgacattca gctcgaccgc gagaaggcca aggcgatggg tgtgccgatc aattcggtgt tcaccgccat gcaggcaacc tttggcagcc tctatgtgaa tgacttcacg ctctatggcc gcaactatca ggttaacctg caatcggaag cggaattccg ccgcgatccg ggcgatctca agcatgtttt cgtgcgcgcc gattccggca gcatgatccc tcttgatgcg ctggtgacgg tgaagcgtat tgtcggtccc gaccagcttg aacgtttcaa tgcgttcaac gccgcaaagg tcaccggcaa cccggctccg ggctatacgt cgggtgatgc gatcaaggcc atgcaggaag tggcggcgca agttctgccg cagggctacc agatcgcatg gaccggctcg gcctatcagg aagtctccac cagcggcacc ggctcgcagg cgatgatctt cggccttatc atggtgttcc tgattctcgc tgcgcaatat gaacgctgga gcctgccgct tgcggttatc accgccgtgc ctttcgccat cttcggtgcg cttctggcga ccgatctgcg cgggcttacc aatgacgtct atttccagat cggtctggtg acgctcatcg gtcttgcggc gaaaaacgcg atcctgatcg tggagttcgc ggtgctggag cgtgagtcgg gtaaatccgc aatcgaggcg gcggcatcgg cggcgcgcct gcgcttccgc ccgatcgtga tgacgtcgct tgccttcatc ctgggcgtgg tgccgcttgc ggtcagcacc ggggctggct cggccagccg tcattccatc ggtacgggcg tgatcggcgg tatgctggcg gcgaccttca tcgcaacctt cttcatcccg atgttctaca gcctgatcgc gcgcaagccg ccgaagaagc atgaggatga gacagccgat acacccgaaa cgccgggcgg tacgggcggc ggtatttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t