SSGCID: Target Page

BrsuA.18198.a

Transcriptional regulator, DeoR family

CENTER ID:	BrsuA.18198.a
ORGANISM:	Brucella suis 1330
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuA.18198.a.B1.GE36828	Full length( BrsuA.18198.a )		1	294

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruSui107850_3073
BV-BRC:	fig\|204722.5.peg.3073
RefSeq:	NP_700025.1
RefSeq:	YP_005614533.1
UniProt:	A0A0H3GBT1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAVSNKASIE NLMTNGLTTG QVPNDSRHAR QLVRRQQIAE TVMAEGSMQI EDLTERFGIS LMTAHRDVDE LVSRGLFRKS RGIVSAAPTS LIEASDLYRV TRQSEEKKLI AEAAMQFVEP GQAIFFDDST TVLQMVPHLP GKGPLTVITN SLILMNEVRD MKDVTLLGLG GQLYNWCNAF VGGMTIHEIR RLRADVAFIS IAAITDDLLF HQSPEMVETK RAMLDCAARR ILLADHTKFE RRALHNFGAL SDFDVVIVDE KTPFAHIERM QAQGINVVVA RASEKVDVIP ARRR

NT Sequence

ATGGCAGTGA GCAACAAGGC AAGCATTGAA AACCTGATGA CGAATGGCCT CACAACGGGG CAGGTGCCGA ACGACAGCCG GCATGCGCGT CAGCTTGTTC GCCGCCAGCA GATTGCGGAA ACTGTTATGG CCGAAGGCTC GATGCAGATC GAGGATCTGA CCGAGCGTTT TGGCATCAGC CTGATGACCG CGCATCGCGA TGTTGATGAA CTGGTCAGCC GCGGCCTGTT CCGCAAATCG CGCGGCATTG TATCGGCTGC GCCGACAAGC CTCATCGAGG CGAGCGATCT ATATCGCGTC ACCCGTCAGT CGGAAGAAAA GAAGCTGATC GCCGAAGCCG CGATGCAGTT TGTCGAGCCG GGACAGGCGA TTTTCTTCGA CGATTCCACA ACCGTCTTGC AAATGGTGCC GCATCTTCCG GGCAAGGGGC CGCTTACCGT CATCACCAAC TCGCTCATCT TGATGAACGA AGTGCGAGAC ATGAAGGATG TGACGCTGCT GGGCCTCGGC GGGCAGCTTT ATAACTGGTG CAATGCTTTT GTCGGCGGCA TGACGATCCA TGAAATTCGC CGTCTTCGTG CCGATGTGGC CTTCATTTCG ATTGCTGCGA TCACCGACGA TCTTTTGTTC CACCAGTCGC CTGAAATGGT CGAAACCAAG CGCGCCATGC TGGACTGTGC GGCCAGGCGC ATCCTGCTGG CCGATCACAC CAAGTTCGAG CGCCGCGCCC TGCACAATTT CGGCGCGCTC AGCGATTTCG ATGTCGTGAT CGTCGATGAA AAAACGCCTT TCGCGCATAT CGAGCGGATG CAGGCGCAAG GCATCAATGT CGTCGTCGCC CGCGCGTCGG AAAAGGTGGA TGTCATACCC GCCAGGCGGC GC

Details for BrsuA.18198.a.B1.GE36828

HARVESTED ON:	3/27/2013
SEQUENCED ON:	3/30/2013
EXPECTED MW:	34kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMA VSNKASIENL MTNGLTTGQV PNDSRHARQL VRRQQIAETV MAEGSMQIED LTERFGISLM TAHRDVDELV SRGLFRKSRG IVSAAPTSLI EASDLYRVTR QSEEKKLIAE AAMQFVEPGQ AIFFDDSTTV LQMVPHLPGK GPLTVITNSL ILMNEVRDMK DVTLLGLGGQ LYNWCNAFVG GMTIHEIRRL RADVAFISIA AITDDLLFHQ SPEMVETKRA MLDCAARRIL LADHTKFERR ALHNFGALSD FDVVIVDEKT PFAHIERMQA QGINVVVARA SEKVDVX

Validated NT Sequence

gggtangaca tccacctttt ccgacgcgcg ggcgacgacg acattgatgc cttgcgcctg catccgctcg atatgcgcga aaggcgtttt ttcatcgacg atcacgacat cgaaatcgct gagcgcgccg aaattgtgca gggcgcggcg ctcgaacttg gtgtgatcgg ccagcaggat gcgcctggcc gcacagtcca gcatggcgcg cttggtttcg accatttcag gcgactggtg gaacaaaaga tcgtcggtga tcgcagcaat cgaaatgaag gccacatcgg cacgaagacg gcgaatttca tggatcgtca tgccgccgac aaaagcattg caccagttat aaagctgccc gccgaggccc agcagcgtca catccttcat gtctcgcact tcgttcatca agatgagcga gttggtgatg acggtaagcg gccccttgcc cggaagatgc ggcaccattt gcaagacggt tgtggaatcg tcgaagaaaa tcgcctgtcc cggctcgaca aactgcatcg cggcttcggc gatcagcttc ttttcttccg actgacgggt gacgcgatat agatcgctcg cctcgatgag gcttgtcggc gcagccgata caatgccgcg cgatttgcgg aacaggccgc ggctgaccag ttcatcaaca tcgcgatgcg cggtcatcag gctgatgcca aaacgctcgg tcagatcctc gatctgcatc gagccttcgg ccataacagt ttccgcaatc tgctggcggc gaacaagctg acgcgcatgc cggctgtcgt tcggcacctg ccccgttgtg aggccattcg tcatcaggtt ttcaatgctt gccttgttgc tcactgccat atggtggtgg tggtggtgag ccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcag tgagcaacaa ggcaagcatt gaaaacctga tgacgaatgg cctcacaacg gggcaggtgc cgaacgacag ccggcatgcg cgtcagcttg ttcgccgcca gcagattgcg gaaactgtta tggccgaagg ctcgatgcag atcgaggatc tgaccgagcg ttttggcatc agcctgatga ccgcgcatcg cgatgttgat gaactggtca gccgcggcct gttccgcaaa tcgcgcggca ttgtatcggc tgcgccgaca agcctcatcg aggcgagcga tctatatcgc gtcacccgtc agtcggaaga aaagaagctg atcgccgaag ccgcgatgca gtttgtcgag ccgggacagg cgattttctt cgacgattcc acaaccgtct tgcaaatggt gccgcatctt ccgggcaagg ggccgcttac cgtcatcacc aactcgctca tcttgatgaa cgaagtgcga gacatgaagg atgtgacgct gctgggcctc ggcgggcagc tttataactg gtgcaatgct tttgtcggcg gcatgacgat ccatgaaatt cgccgtcttc gtgccgatgt ggccttcatt tcgattgctg cgatcaccga cgatcttttg ttccaccagt cgcctgaaat ggtcgaaacc aagcgcgcca tgctggactg tgcggccagg cgcatcctgc tggccgatca caccaagttc gagcgccgcg ccctgcacaa tttcggcgcg ctcagcgatt tcgatgtcgt gatcgtcgat gaaaaaacgc ctttcgcgca tatcgagcgg atgcaggcgc aaggcatcaa tgtcgtcgtc gcccgcgcgt cggaaaaggt ggatgtcata cccgccaggc ggcgctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat