BrsuA.18180.b

Galactonate dehydratase (EC 4.2.1.6)

CENTER ID:	BrsuA.18180.b
ORGANISM:	Brucella suis 1330
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	native diffraction-data
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuA.18180.b.B1.GE36826	Full length( BrsuA.18180.b )		1	613

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|204722.5.peg.3159
RefSeq:	NP_700107.1
RefSeq:	YP_005614614.1
UniProt:	A0A0H3GG12

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFSNLLPTQD SEMNKPVTPK TPKLRSRAWF DNPDNVDMTA LYLERYMNYG LSQEELQSGR PIIGIAQTGS DLSPCNRHHL ELAKRVRDGV REAGGIVIEF PVHPIQETGK RPTAGLDRNL AYLGLVEVLY GYPLDGVVLT IGCDKTTPAC LMAAATVNIP AIALSVGPML NGWFRGERTG SGTIVWKARE LLAKGEIDYQ GFVKLVASSA PSTGYCNTMG TATTMNSLAE ALGMQLPGSA AIPAPYRDRQ EVAYLTGRRI VEMVHEDLKP SDILTKEAFI NAIRVNSAIG GSTNAPIHLN ALARHIGVEL TVDDWQKYGE EIPLLVNLQP AGEYLGEDYY HAGGVPAVVN QLMGQGLIHE DAITVNGKTI GENCKNATIE DSNVIKTYDQ PLKKHAGFRV LRGNLFSSAI MKLSVISDEF RNRYLSDAKD PNAFEGKAVV FDGPEDYHHR IDDPALEIDE HTVLFMRGAG PIGYPGAAEV VNMRAPDYLL KKGITSLPCI GDGRQSGTSG SPSILNASPE AAAGGGLAIL KTGDRVRIDL GRGTADNLIS DEELAERRKA LEAVGGYKYP ESQTPWQEIQ RAVIGQMETG AVLENAVKYQ DIAHTRGLPR DNH

NT Sequence

TTGTTTTCCA ATCTCTTACC CACGCAGGAC AGTGAAATGA ACAAGCCGGT TACGCCAAAA ACACCCAAAT TGCGGTCACG CGCATGGTTC GACAATCCCG ACAATGTCGA TATGACGGCG CTCTATCTTG AGCGTTACAT GAATTACGGG CTGAGCCAGG AAGAGCTGCA ATCCGGCCGT CCCATCATCG GTATTGCGCA AACCGGTTCT GATCTTTCGC CCTGCAACCG GCATCATCTG GAACTGGCCA AGCGTGTGAG GGATGGAGTT CGCGAGGCGG GTGGCATCGT CATCGAATTT CCGGTTCATC CCATTCAGGA AACCGGCAAG CGCCCCACTG CGGGTCTCGA CCGCAATCTG GCCTATCTTG GTCTGGTCGA AGTGCTTTAC GGCTATCCGC TCGATGGTGT GGTGCTGACC ATCGGCTGCG ACAAGACCAC GCCCGCCTGC CTGATGGCAG CGGCGACCGT CAATATTCCG GCCATCGCGC TGTCGGTCGG GCCGATGCTG AACGGCTGGT TCCGGGGTGA GCGCACGGGT TCGGGCACCA TCGTCTGGAA GGCGCGCGAA CTGCTTGCCA AGGGCGAAAT CGACTATCAG GGCTTTGTGA AACTGGTGGC TTCTTCGGCT CCATCGACCG GCTATTGCAA CACGATGGGC ACGGCCACGA CCATGAATTC TCTGGCCGAA GCGCTGGGTA TGCAGCTTCC GGGCTCGGCG GCTATCCCCG CACCTTATCG TGACCGTCAG GAAGTCGCCT ATCTGACGGG GCGGCGTATT GTCGAGATGG TTCATGAAGA CCTGAAACCA TCGGATATCC TGACGAAGGA AGCCTTTATC AATGCGATCC GCGTCAATTC CGCCATTGGC GGCTCCACCA ATGCGCCGAT CCATCTCAAT GCGCTCGCGC GCCATATCGG TGTGGAACTG ACGGTGGATG ACTGGCAGAA ATATGGCGAG GAAATTCCGC TTCTGGTCAA TCTCCAGCCT GCGGGCGAAT ATCTGGGCGA AGACTATTAT CACGCAGGCG GTGTGCCAGC GGTGGTCAAC CAGTTGATGG GGCAGGGCCT CATCCATGAG GACGCAATCA CGGTCAATGG CAAGACCATC GGCGAAAATT GCAAGAATGC GACCATCGAA GACAGCAATG TCATCAAGAC CTACGATCAG CCGCTCAAGA AACATGCCGG TTTCCGCGTG CTGCGCGGCA ATCTGTTCTC GTCGGCCATC ATGAAGCTCA GCGTGATTTC GGACGAATTC CGCAATCGCT ACCTGTCCGA TGCCAAGGAT CCCAATGCCT TTGAAGGCAA GGCGGTCGTG TTCGATGGGC CGGAGGATTA TCATCACCGT ATTGATGATC CGGCGCTGGA AATAGATGAA CATACGGTGC TTTTCATGCG CGGTGCGGGA CCGATCGGCT ATCCGGGTGC GGCGGAAGTC GTCAACATGC GCGCGCCGGA TTACCTTTTG AAGAAGGGCA TCACGTCGCT GCCCTGTATC GGCGACGGCC GCCAGTCGGG TACGTCGGGT TCGCCGTCGA TCCTCAATGC CTCGCCGGAA GCGGCGGCAG GCGGGGGGCT TGCCATTCTC AAGACCGGCG ACCGCGTGCG CATCGATCTT GGCCGCGGGA CGGCGGATAA CCTGATTTCC GATGAGGAAT TGGCCGAACG CCGCAAGGCG CTGGAAGCGG TTGGCGGTTA TAAATATCCT GAAAGCCAGA CGCCATGGCA GGAAATCCAG CGTGCTGTCA TCGGCCAGAT GGAAACCGGG GCCGTGCTGG AAAACGCGGT CAAATATCAG GATATCGCGC ATACGCGCGG GCTTCCGCGA GATAACCAT

Details for BrsuA.18180.b.B1.GE36826

HARVESTED ON:	3/27/2013
SEQUENCED ON:	3/30/2013
EXPECTED MW:	68kDa
OBSERVED MW:	68kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	95
PERCENT COVERAGE:	51

Validated AA Sequence

MAHHHHHHML FSNLLPTQDS EMNKPVTPKT PKLRSRAWFD NPDNVDMTAL YLERYMNYGL SQEELQSGRP IIGIAQTGSD LSPCNRHHLE LAKRVRDGVR EAGGIVIEFP VHPIQETGKR PTAGLDRNLA YLGLVEVLYG YPLDGVVLTI GCDKTTPACL MAAATVNIPA IALSVGPMLN GWFRGERTGS GTIVWKAREL LAKGEIDYQG FVKLVASSAP STGYCNTMGT ATTMNSLAEA LGMQLPGSAA IPAPYRDRQE VAYLTGRRXV EMVHEDLKPS DIXTXXAFIN AIRVNSAIGG STXAPIHLNX LARXSXXXXX X

Validated NT Sequence

tttgnttaac tttaagaagg aganatacca tggctcacca ccaccaccac catatgttgt tttccaatct cttacccacg caggacagtg aaatgaacaa gccggttacg ccaaaaacac ccaaattgcg gtcacgcgca tggttcgaca atcccgacaa tgtcgatatg acggcgctct atcttgagcg ttacatgaat tacgggctga gccaggaaga gctgcaatcc ggccgtccca tcatcggtat tgcgcaaacc ggttctgatc tttcgccctg caaccggcat catctggaac tggccaagcg tgtgagggat ggagttcgcg aggcgggtgg catcgtcatc gaatttccgg ttcatcccat tcaggaaacc ggcaagcgcc ccactgcggg tctcgaccgc aatctggcct atcttggtct ggtcgaagtg ctttacggct atccgctcga tggtgtggtg ctgaccatcg gctgcgacaa gaccacgccc gcctgcctga tggcagcggc gaccgtcaat attccggcca tcgcgctgtc ggtcgggccg atgctgaacg gctggttccg gggtgagcgc acgggttcgg gcaccatcgt ctggaaggcg cgcgaactgc ttgccaaggg cgaaatcgac tatcagggct ttgtgaaact ggtggcttct tcggctccat cgaccggcta ttgcaacacg atgggcacgg ccacgaccat gaattctctg gccgaagcgc tgggtatgca gcttccgggc tcggcggcta tccccgcacc ttatcgtgac cgtcaggaag tcgcctatct gacggggcgg cgtantgtcg agatggttca tgaagacctg aaaccatcgg atatcntgac nnnnnnagcc tttatcaatg cgatccgcgt caattccgcc attggcggct ccaccnatgc gccnatccat ctcaatgnnc tcgcgcgcnt atcggngtnn nctgangntn gntgactgnn nnaannnnng gnnnngaaat tcngcttcnn gncnnncnnn cancnnncng nnnaannnnn nggnnannnn gnnnnnncnn cnnnnnnnnn nnnnntggnn nnnnnnnnnc nnnnnnnngn nnnnnnnnnn nnnnntngnn nnnnnncann nnnnnnnnnn nnnnnnnnna ncnnnnnnnn nnnncnnnna nnaa

Expected Protein Sequence

MAHHHHHHMF SNLLPTQDSE MNKPVTPKTP KLRSRAWFDN PDNVDMTALY LERYMNYGLS QEELQSGRPI IGIAQTGSDL SPCNRHHLEL AKRVRDGVRE AGGIVIEFPV HPIQETGKRP TAGLDRNLAY LGLVEVLYGY PLDGVVLTIG CDKTTPACLM AAATVNIPAI ALSVGPMLNG WFRGERTGSG TIVWKARELL AKGEIDYQGF VKLVASSAPS TGYCNTMGTA TTMNSLAEAL GMQLPGSAAI PAPYRDRQEV AYLTGRRIVE MVHEDLKPSD ILTKEAFINA IRVNSAIGGS TNAPIHLNAL ARHIGVELTV DDWQKYGEEI PLLVNLQPAG EYLGEDYYHA GGVPAVVNQL MGQGLIHEDA ITVNGKTIGE NCKNATIEDS NVIKTYDQPL KKHAGFRVLR GNLFSSAIMK LSVISDEFRN RYLSDAKDPN AFEGKAVVFD GPEDYHHRID DPALEIDEHT VLFMRGAGPI GYPGAAEVVN MRAPDYLLKK GITSLPCIGD GRQSGTSGSP SILNASPEAA AGGGLAILKT GDRVRIDLGR GTADNLISDE ELAERRKALE AVGGYKYPES QTPWQEIQRA VIGQMETGAV LENAVKYQDI AHTRGLPRDN H

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgtttt ccaatctctt acccacgcag gacagtgaaa tgaacaagcc ggttacgcca aaaacaccca aattgcggtc acgcgcatgg ttcgacaatc ccgacaatgt cgatatgacg gcgctctatc ttgagcgtta catgaattac gggctgagcc aggaagagct gcaatccggc cgtcccatca tcggtattgc gcaaaccggt tctgatcttt cgccctgcaa ccggcatcat ctggaactgg ccaagcgtgt gagggatgga gttcgcgagg cgggtggcat cgtcatcgaa tttccggttc atcccattca ggaaaccggc aagcgcccca ctgcgggtct cgaccgcaat ctggcctatc ttggtctggt cgaagtgctt tacggctatc cgctcgatgg tgtggtgctg accatcggct gcgacaagac cacgcccgcc tgcctgatgg cagcggcgac cgtcaatatt ccggccatcg cgctgtcggt cgggccgatg ctgaacggct ggttccgggg tgagcgcacg ggttcgggca ccatcgtctg gaaggcgcgc gaactgcttg ccaagggcga aatcgactat cagggctttg tgaaactggt ggcttcttcg gctccatcga ccggctattg caacacgatg ggcacggcca cgaccatgaa ttctctggcc gaagcgctgg gtatgcagct tccgggctcg gcggctatcc ccgcacctta tcgtgaccgt caggaagtcg cctatctgac ggggcggcgt attgtcgaga tggttcatga agacctgaaa ccatcggata tcctgacgaa ggaagccttt atcaatgcga tccgcgtcaa ttccgccatt ggcggctcca ccaatgcgcc gatccatctc aatgcgctcg cgcgccatat cggtgtggaa ctgacggtgg atgactggca gaaatatggc gaggaaattc cgcttctggt caatctccag cctgcgggcg aatatctggg cgaagactat tatcacgcag gcggtgtgcc agcggtggtc aaccagttga tggggcaggg cctcatccat gaggacgcaa tcacggtcaa tggcaagacc atcggcgaaa attgcaagaa tgcgaccatc gaagacagca atgtcatcaa gacctacgat cagccgctca agaaacatgc cggtttccgc gtgctgcgcg gcaatctgtt ctcgtcggcc atcatgaagc tcagcgtgat ttcggacgaa ttccgcaatc gctacctgtc cgatgccaag gatcccaatg cctttgaagg caaggcggtc gtgttcgatg ggccggagga ttatcatcac cgtattgatg atccggcgct ggaaatagat gaacatacgg tgcttttcat gcgcggtgcg ggaccgatcg gctatccggg tgcggcggaa gtcgtcaaca tgcgcgcgcc ggattacctt ttgaagaagg gcatcacgtc gctgccctgt atcggcgacg gccgccagtc gggtacgtcg ggttcgccgt cgatcctcaa tgcctcgccg gaagcggcgg caggcggggg gcttgccatt ctcaagaccg gcgaccgcgt gcgcatcgat cttggccgcg ggacggcgga taacctgatt tccgatgagg aattggccga acgccgcaag gcgctggaag cggttggcgg ttataaatat cctgaaagcc agacgccatg gcaggaaatc cagcgtgctg tcatcggcca gatggaaacc ggggccgtgc tggaaaacgc ggtcaaatat caggatatcg cgcatacgcg cgggcttccg cgagataacc attgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat