BrsuA.18179.a

2-isopropylmalate synthase (EC 2.3.3.13) (Alpha-IPM synthase) (Alpha-isopropylmalate synthase)

CENTER ID: BrsuA.18179.a
ORGANISM: Brucella suis 1330
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuA.18179.a.A1.GE33968 full length 1 555
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuA.18179.a.A1.PS01442 full length 1 555
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_132150
PATRIC ID: fig|204722.5.peg.1593
RefSeq: NP_698557.2
UniProt: Q8FZC4
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPIAGTKYAP FPAPQLDDRT WPSKRIEKAP IWCSVDLRDG NQALIDPMGH DRKERMFRLL IDMGFPEIEI GFPSASQTDF DFCRWAIEQG DVPDDVDLQV LVQCRPELIT RTFEALEGAK TPIIHFYNST SELQRRVVFA KDVGGIKQIA TDAAKMIMDM AAKAGGGYRF QYSPESFTGT ELDVALEICN AVIEIVKPTP DNKLIVNLPS TVEMNTPNVY ADQIEWMCRN LDNRESLIIS LHPHNDRGTG IAATELGLMA GADRVEGTLF GNGERTGNVD VVTLALNMYT QGIDPGLDCT DINRMKEVYE YSNQLKIAER HPYVGELVYT AFSGSHQDAI NKGMKARRSA NSPVWEVPYL PIDPQDVGRS YEAIIRINSQ SGKGGIAYIL QADYGLNLPR NLQVEFREII QHITDEEGKE LPSKRIYEEF QKLYVTQPDA RIKFVDHHTY PDPEQKGRRI LTAEITDNGV TKTIEGKGTG PIDGFVDALS KYLGVKMSVV DYSEHSLQQG SDASAISYVE MVYPGGKLFG VGINDNIVSA SLEAVVSAAN RVIAK
NT Sequence
atgaaccagc ctgtctccac gccaaagtcc gtgtcccaag ccgtatcggc acgcaagggc atgcctattg ccggtaccaa atacgcgccg ttccctgcgc cgcagctcga tgaccgcact tggccttcca aacgcatcga aaaggcgccg atctggtgtt cggtcgatct gcgcgacggc aaccaggctt tgatcgaccc catggggcat gaccgcaagg agcgcatgtt ccgcctgctg atcgatatgg gtttcccgga aatcgagatc ggtttcccgt ccgcttcgca gacggatttc gacttctgcc gctgggccat tgaacagggc gatgttcctg acgacgtgga tttgcaggtt ctggtccagt gccgtcctga attgattacg cgcactttcg aggcgctgga aggcgcgaag acgccgatca tccatttcta caattccacc agcgaattgc agcgccgcgt ggtgtttgcc aaggatgtcg gcggcatcaa gcagattgca accgatgcgg ccaagatgat catggatatg gctgccaagg ctggcggcgg ttatcgcttc cagtattcgc cggaaagctt taccggcacc gaactggatg tggcgctgga aatctgcaat gcggtgatcg agatcgtgaa gccgacgccg gacaacaagc tgatcgtcaa cctgccttcg acggtggaga tgaacacgcc gaatgtctat gccgaccaga tcgaatggat gtgccgcaat ctggacaatc gcgagagcct gattatctcg ctgcatccgc ataatgaccg cggcacgggc attgccgcca ctgaactggg cctgatggca ggcgctgatc gcgtggaagg cacgctgttc ggcaatggcg agcgcacggg caatgtggat gtggtgacgc tggcgctcaa catgtacacg cagggcatcg accccgggct ggattgcacc gatatcaacc ggatgaagga agtctacgaa tattccaacc agttgaagat cgccgagcgc catccttatg tgggcgagct ggtctatacg gctttctccg gctcgcatca ggatgcgatc aacaagggca tgaaagcgcg ccgttcggcc aattcgccgg tttgggaagt tccttacctg ccgatcgatc cgcaggatgt gggccgctcc tacgaggcga tcatccgcat caattcgcaa tcgggcaagg gcggtatcgc ctatatcctg caagccgatt acggcctgaa cctgccgcgc aacctgcagg tcgagttccg cgaaatcatc cagcacatta cggatgaaga gggcaaggag ctgccatcga agcgcattta cgaggagttc cagaaactct atgtgacgca gcccgatgcc cgcatcaagt tcgtggatca ccacacctat cccgatcctg agcaaaaggg ccggcgcatc ctgacggctg agatcaccga taacggtgtc accaagacta tcgagggcaa gggcacggga ccgatcgatg gtttcgtgga tgcgctgtcg aaatatcttg gcgtgaaaat gtcggtggtc gattattccg aacattcgct ccagcagggt tcggatgctt cggccatttc ctatgtcgag atggtttatc ccggcggcaa gctgttcggt gtcggcatca acgataatat cgtcagcgcc tcgcttgaag ccgtggtgtc ggcggcaaat cgtgtgattg cgaagtag
Details for BrsuA.18179.a.A1.GE33968
HARVESTED ON: 11/21/2011
SEQUENCED ON: 11/29/2011
EXPECTED MW: 63kDa
OBSERVED MW: 63kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 91
PERCENT COVERAGE: 52
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNQPVSTPK SVSQAVSARK GMPIAGTKYA PFPAPQLDDR TWPSKRIEKA PIWCSVDLRD GNQALIDPMG HDRKERMFRL LIDMGFPEIE IGFPSASQTD FDFCRWAIEQ GDVPDDVDLQ VLVQCRPELI TRTFEALEGA KTPIIHFYNS TSELQRRVVF AKDVGGIKQI ATDAAKMIMD MAAKAGGGYR FQYSPESFTG TELDVALEIC NAVIEXVKPT PDNKLIVNLP STVEXNTPXX YADQIEXXCX NLDNREXXXX XCXXXMXAAR XXXPXNWXDG XR
Validated NT Sequence
atagtcttgg tgacaccgtt atcggtgatc tcagccgtca ggatgcgccg gcccttttgc tcaggatcgg gataggtgtg gtgatccacg aacttgatgc gggcatcggg ctgcgtcaca tagagtttct ggaactcctc gtaaatgcgc ttcgatggca gctccttgcc ctcttcatcc gtaatgtgct ggatgatttc gcggaactcg acctgcaggt tgcgcggcag gttcaggccg taatcggctt gcaggatata ggcgataccg cccttgcccg attgcgaatt gatgcggatg atcgcctcgt aggagcggcc cacatcctgc ggatcgatcg gcaggtaagg aacttcccaa accggcgaat tggccgaacg gcgcgctttc atgcccttgt tgatcgcatc ctgatgcgag ccggagaaag ccgtatagac cagctcgccc acataaggat ggcgctcggc gatcttcaac tggttggaat attcgtagac ttccttcatc cggttgatat cggtgcaatc cagcccgggg tcgatgccct gcgtgtacat gttgagcgcc agcgtcacca catccacatt gcccgtgcgc tcgccattgc cgaacagcgt gncttccncg cgatcagcgc cngccatcnn nccagttcan tggcnnnnan gnccgtgccg cgnncattan gnnnatgcag cnannnnatc nnntctcgcg attgtccaga ttgnggcacn nccnttcgat ctggtcggca tagncnttcg gcgtgttcnt ctccaccgtn ganggcaggt tgacgatcag cttgttgtcc ggcgtnggct tcacgntctc gatcaccgca ttgcagattt ccagcgccac atccagttcg gtgccggtaa agctttccgg cgaatactgg aagcgataac cgccgccagc cttggcagcc atatccatga tcatcttggc cgcatcggtt gcaatctgct tgatgccgcc gacatccttg gcaaacacca cgcggcgctg caattcgctg gtggaattgt agaaatggat gatcggcgtc ttcgcgcctt ccagcgcctc gaaagtgcgc gtaatcaatt caggacggca ctggaccaga acctgcaaat ccacgtcgtc aggaacatcg ccctgttcaa tggcccagcg gcagaagtcg aaatccgtct gcgaagcgga cgggaaaccg atctcgattt ccgggaaacc catatcgatc agcaggcgga acatgcgctc cttgcggtca tgccccatgg ggtcgatcaa agcctggttg ccgtcgcgca gatcgaccga acaccagatc ggcgcctttt cgatgcgttt ggaaggccaa gtgcggtcat cgagctgcgg cgcagggaac ggcgcgtatt tggtaccggc aataggcatg cccttgcgtg ccgatacggc ttgggacacg gactttggcg tggagacagg ctggttcatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNQPVSTPK SVSQAVSARK GMPIAGTKYA PFPAPQLDDR TWPSKRIEKA PIWCSVDLRD GNQALIDPMG HDRKERMFRL LIDMGFPEIE IGFPSASQTD FDFCRWAIEQ GDVPDDVDLQ VLVQCRPELI TRTFEALEGA KTPIIHFYNS TSELQRRVVF AKDVGGIKQI ATDAAKMIMD MAAKAGGGYR FQYSPESFTG TELDVALEIC NAVIEIVKPT PDNKLIVNLP STVEMNTPNV YADQIEWMCR NLDNRESLII SLHPHNDRGT GIAATELGLM AGADRVEGTL FGNGERTGNV DVVTLALNMY TQGIDPGLDC TDINRMKEVY EYSNQLKIAE RHPYVGELVY TAFSGSHQDA INKGMKARRS ANSPVWEVPY LPIDPQDVGR SYEAIIRINS QSGKGGIAYI LQADYGLNLP RNLQVEFREI IQHITDEEGK ELPSKRIYEE FQKLYVTQPD ARIKFVDHHT YPDPEQKGRR ILTAEITDNG VTKTIEGKGT GPIDGFVDAL SKYLGVKMSV VDYSEHSLQQ GSDASAISYV EMVYPGGKLF GVGIND
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaccagcct gtctccacgc caaagtccgt gtcccaagcc gtatcggcac gcaagggcat gcctattgcc ggtaccaaat acgcgccgtt ccctgcgccg cagctcgatg accgcacttg gccttccaaa cgcatcgaaa aggcgccgat ctggtgttcg gtcgatctgc gcgacggcaa ccaggctttg atcgacccca tggggcatga ccgcaaggag cgcatgttcc gcctgctgat cgatatgggt ttcccggaaa tcgagatcgg tttcccgtcc gcttcgcaga cggatttcga cttctgccgc tgggccattg aacagggcga tgttcctgac gacgtggatt tgcaggttct ggtccagtgc cgtcctgaat tgattacgcg cactttcgag gcgctggaag gcgcgaagac gccgatcatc catttctaca attccaccag cgaattgcag cgccgcgtgg tgtttgccaa ggatgtcggc ggcatcaagc agattgcaac cgatgcggcc aagatgatca tggatatggc tgccaaggct ggcggcggtt atcgcttcca gtattcgccg gaaagcttta ccggcaccga actggatgtg gcgctggaaa tctgcaatgc ggtgatcgag atcgtgaagc cgacgccgga caacaagctg atcgtcaacc tgccttcgac ggtggagatg aacacgccga atgtctatgc cgaccagatc gaatggatgt gccgcaatct ggacaatcgc gagagcctga ttatctcgct gcatccgcat aatgaccgcg gcacgggcat tgccgccact gaactgggcc tgatggcagg cgctgatcgc gtggaaggca cgctgttcgg caatggcgag cgcacgggca atgtggatgt ggtgacgctg gcgctcaaca tgtacacgca gggcatcgac cccgggctgg attgcaccga tatcaaccgg atgaaggaag tctacgaata ttccaaccag ttgaagatcg ccgagcgcca tccttatgtg ggcgagctgg tctatacggc tttctccggc tcgcatcagg atgcgatcaa caagggcatg aaagcgcgcc gttcggccaa ttcgccggtt tgggaagttc cttacctgcc gatcgatccg caggatgtgg gccgctccta cgaggcgatc atccgcatca attcgcaatc gggcaagggc ggtatcgcct atatcctgca agccgattac ggcctgaacc tgccgcgcaa cctgcaggtc gagttccgcg aaatcatcca gcacattacg gatgaagagg gcaaggagct gccatcgaag cgcatttacg aggagttcca gaaactctat gtgacgcagc ccgatgcccg catcaagttc gtggatcacc acacctatcc cgatcctgag caaaagggcc ggcgcatcct gacggctgag atcaccgata acggtgtcac caagactatc gagggcaagg gcacgggacc gatcgatggt ttcgtggatg cgctgtcgaa atatcttggc gtgaaaatgt cggtggtcga ttattccgaa cattcgctcc agcagggttc ggatgcttcg gccatttcct atgtcgagat ggtttatccc ggcggcaagc tgttcggtgt cggcatcaac gattaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for BrsuA.18179.a.A1.PS01442
PURIFICATION DATe: 3/29/2012
CONCENTRATION: 27.3mg/ml
OBSERVED MW: 64kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 110µl
PERCENT IDENTITY: 91
PERCENT COVERAGE: 52
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNQPVSTPK SVSQAVSARK GMPIAGTKYA PFPAPQLDDR TWPSKRIEKA PIWCSVDLRD GNQALIDPMG HDRKERMFRL LIDMGFPEIE IGFPSASQTD FDFCRWAIEQ GDVPDDVDLQ VLVQCRPELI TRTFEALEGA KTPIIHFYNS TSELQRRVVF AKDVGGIKQI ATDAAKMIMD MAAKAGGGYR FQYSPESFTG TELDVALEIC NAVIEXVKPT PDNKLIVNLP STVEXNTPXX YADQIEXXCX NLDNREXXXX XCXXXMXAAR XXXPXNWXDG XR
Validated NT Sequence
atagtcttgg tgacaccgtt atcggtgatc tcagccgtca ggatgcgccg gcccttttgc tcaggatcgg gataggtgtg gtgatccacg aacttgatgc gggcatcggg ctgcgtcaca tagagtttct ggaactcctc gtaaatgcgc ttcgatggca gctccttgcc ctcttcatcc gtaatgtgct ggatgatttc gcggaactcg acctgcaggt tgcgcggcag gttcaggccg taatcggctt gcaggatata ggcgataccg cccttgcccg attgcgaatt gatgcggatg atcgcctcgt aggagcggcc cacatcctgc ggatcgatcg gcaggtaagg aacttcccaa accggcgaat tggccgaacg gcgcgctttc atgcccttgt tgatcgcatc ctgatgcgag ccggagaaag ccgtatagac cagctcgccc acataaggat ggcgctcggc gatcttcaac tggttggaat attcgtagac ttccttcatc cggttgatat cggtgcaatc cagcccgggg tcgatgccct gcgtgtacat gttgagcgcc agcgtcacca catccacatt gcccgtgcgc tcgccattgc cgaacagcgt gncttccncg cgatcagcgc cngccatcnn nccagttcan tggcnnnnan gnccgtgccg cgnncattan gnnnatgcag cnannnnatc nnntctcgcg attgtccaga ttgnggcacn nccnttcgat ctggtcggca tagncnttcg gcgtgttcnt ctccaccgtn ganggcaggt tgacgatcag cttgttgtcc ggcgtnggct tcacgntctc gatcaccgca ttgcagattt ccagcgccac atccagttcg gtgccggtaa agctttccgg cgaatactgg aagcgataac cgccgccagc cttggcagcc atatccatga tcatcttggc cgcatcggtt gcaatctgct tgatgccgcc gacatccttg gcaaacacca cgcggcgctg caattcgctg gtggaattgt agaaatggat gatcggcgtc ttcgcgcctt ccagcgcctc gaaagtgcgc gtaatcaatt caggacggca ctggaccaga acctgcaaat ccacgtcgtc aggaacatcg ccctgttcaa tggcccagcg gcagaagtcg aaatccgtct gcgaagcgga cgggaaaccg atctcgattt ccgggaaacc catatcgatc agcaggcgga acatgcgctc cttgcggtca tgccccatgg ggtcgatcaa agcctggttg ccgtcgcgca gatcgaccga acaccagatc ggcgcctttt cgatgcgttt ggaaggccaa gtgcggtcat cgagctgcgg cgcagggaac ggcgcgtatt tggtaccggc aataggcatg cccttgcgtg ccgatacggc ttgggacacg gactttggcg tggagacagg ctggttcatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNQPVSTPK SVSQAVSARK GMPIAGTKYA PFPAPQLDDR TWPSKRIEKA PIWCSVDLRD GNQALIDPMG HDRKERMFRL LIDMGFPEIE IGFPSASQTD FDFCRWAIEQ GDVPDDVDLQ VLVQCRPELI TRTFEALEGA KTPIIHFYNS TSELQRRVVF AKDVGGIKQI ATDAAKMIMD MAAKAGGGYR FQYSPESFTG TELDVALEIC NAVIEIVKPT PDNKLIVNLP STVEMNTPNV YADQIEWMCR NLDNRESLII SLHPHNDRGT GIAATELGLM AGADRVEGTL FGNGERTGNV DVVTLALNMY TQGIDPGLDC TDINRMKEVY EYSNQLKIAE RHPYVGELVY TAFSGSHQDA INKGMKARRS ANSPVWEVPY LPIDPQDVGR SYEAIIRINS QSGKGGIAYI LQADYGLNLP RNLQVEFREI IQHITDEEGK ELPSKRIYEE FQKLYVTQPD ARIKFVDHHT YPDPEQKGRR ILTAEITDNG VTKTIEGKGT GPIDGFVDAL SKYLGVKMSV VDYSEHSLQQ GSDASAISYV EMVYPGGKLF GVGIND
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAACCAGCCT GTCTCCACGC CAAAGTCCGT GTCCCAAGCC GTATCGGCAC GCAAGGGCAT GCCTATTGCC GGTACCAAAT ACGCGCCGTT CCCTGCGCCG CAGCTCGATG ACCGCACTTG GCCTTCCAAA CGCATCGAAA AGGCGCCGAT CTGGTGTTCG GTCGATCTGC GCGACGGCAA CCAGGCTTTG ATCGACCCCA TGGGGCATGA CCGCAAGGAG CGCATGTTCC GCCTGCTGAT CGATATGGGT TTCCCGGAAA TCGAGATCGG TTTCCCGTCC GCTTCGCAGA CGGATTTCGA CTTCTGCCGC TGGGCCATTG AACAGGGCGA TGTTCCTGAC GACGTGGATT TGCAGGTTCT GGTCCAGTGC CGTCCTGAAT TGATTACGCG CACTTTCGAG GCGCTGGAAG GCGCGAAGAC GCCGATCATC CATTTCTACA ATTCCACCAG CGAATTGCAG CGCCGCGTGG TGTTTGCCAA GGATGTCGGC GGCATCAAGC AGATTGCAAC CGATGCGGCC AAGATGATCA TGGATATGGC TGCCAAGGCT GGCGGCGGTT ATCGCTTCCA GTATTCGCCG GAAAGCTTTA CCGGCACCGA ACTGGATGTG GCGCTGGAAA TCTGCAATGC GGTGATCGAG ATCGTGAAGC CGACGCCGGA CAACAAGCTG ATCGTCAACC TGCCTTCGAC GGTGGAGATG AACACGCCGA ATGTCTATGC CGACCAGATC GAATGGATGT GCCGCAATCT GGACAATCGC GAGAGCCTGA TTATCTCGCT GCATCCGCAT AATGACCGCG GCACGGGCAT TGCCGCCACT GAACTGGGCC TGATGGCAGG CGCTGATCGC GTGGAAGGCA CGCTGTTCGG CAATGGCGAG CGCACGGGCA ATGTGGATGT GGTGACGCTG GCGCTCAACA TGTACACGCA GGGCATCGAC CCCGGGCTGG ATTGCACCGA TATCAACCGG ATGAAGGAAG TCTACGAATA TTCCAACCAG TTGAAGATCG CCGAGCGCCA TCCTTATGTG GGCGAGCTGG TCTATACGGC TTTCTCCGGC TCGCATCAGG ATGCGATCAA CAAGGGCATG AAAGCGCGCC GTTCGGCCAA TTCGCCGGTT TGGGAAGTTC CTTACCTGCC GATCGATCCG CAGGATGTGG GCCGCTCCTA CGAGGCGATC ATCCGCATCA ATTCGCAATC GGGCAAGGGC GGTATCGCCT ATATCCTGCA AGCCGATTAC GGCCTGAACC TGCCGCGCAA CCTGCAGGTC GAGTTCCGCG AAATCATCCA GCACATTACG GATGAAGAGG GCAAGGAGCT GCCATCGAAG CGCATTTACG AGGAGTTCCA GAAACTCTAT GTGACGCAGC CCGATGCCCG CATCAAGTTC GTGGATCACC ACACCTATCC CGATCCTGAG CAAAAGGGCC GGCGCATCCT GACGGCTGAG ATCACCGATA ACGGTGTCAC CAAGACTATC GAGGGCAAGG GCACGGGACC GATCGATGGT TTCGTGGATG CGCTGTCGAA ATATCTTGGC GTGAAAATGT CGGTGGTCGA TTATTCCGAA CATTCGCTCC AGCAGGGTTC GGATGCTTCG GCCATTTCCT ATGTCGAGAT GGTTTATCCC GGCGGCAAGC TGTTCGGTGT CGGCATCAAC GATTAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA