SSGCID: Target Page

BrsuA.18174.a

Probable phosphoketolase (EC 4.1.2.-)

CENTER ID:	BrsuA.18174.a
ORGANISM:	Brucella suis 1330
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuA.18174.a.A1.GE33972	full length		1	789

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|204722.5.peg.2597
RefSeq:	NP_699578.1
UniProt:	Q8FWR0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPAKGPLTPQ QLSLINRYWR AANYLSVGQI YLMKNPLLRE PLQPEHIKPR LLGHWGTTPG LNFIYAHLNR IIQQRNANVI YICGPGHGGP GMVANTYLEG TYSEIYPAIS EDEAGMERLF RQFSFPGGIP SHAAPETPGS IHEGGELGYA LVHAYGAAFD NPDLVVACVV GDGEAETGAL ATSWHSNKFL NPARDGAVLP ILHLNGYKIA NPTVLARLSD DDLDNLFRGY GYEPFFVEGS EPADMHQKMA ATLDTIFQRI QDIKKNADVH SPERPRWPMI ILRSPKGWTG PKTVDGLVVE NYWRAHQVPV ANCRENDAHR KILEDWMKSY DPSDLFDEKG ALKPELRALA PKGEARIGAN PHANGGLLRK ELHMPDFRQY AVNVTEPGAI EAQSTKILGD FLRDVMKLNE TEKNFRIFGP DETASNRLGS VLEATNRVWM AETLDMDDHL AADGRVMEVL SEHLCQGWLE GYLLSGRHGF FSCYEAFIHI IDSMFNQHAK WLQVARELEW RKPISSLNYL LTSHVWRQDH NGFSHQDPGF VDLVANKSAD IVRVYFPPDA NTLLWVGDHC LKTWNRVNVI VAGKQPEPQW LTMAEAEKHC EAGLGIWEWA GTEDGLEPDI VMACAGDVPT METLAAVDLL RQSLPHLRIR VVNVVDLMVL QSPHQHPHGI SDEEFDRMFT TNRPVIFAYH GYPYLIHRLV YKRTNHSNFH VRGFIEQGTT TTPFDMTVLN ELDRFHLAME AVERLPLGES VAKPLIDNFT EKLALHKDYI RQHGEDMPEI RDWKWTWPR

NT Sequence

gtgccagcaa aagggcctct cacaccgcag cagctttcac tcatcaaccg ttactggcgc gcggcgaatt atctttccgt cggccagatt tatctgatga aaaatcccct gttgcgcgaa ccgctccagc ctgagcacat caagccgcgg cttctcggcc attggggcac gacacccggc ctcaatttca tctatgcgca tctcaaccgc attattcagc agcgcaacgc caatgtgatc tatatttgcg gccccggcca tggcgggccg ggcatggtgg ccaacaccta tctggagggc acctattccg aaatctatcc cgcaatcagc gaagatgaag cgggcatgga aaggctcttc cgccagtttt ccttccccgg cggaatacca agccatgccg cgccggaaac accgggctct atccacgaag ggggcgaact gggttatgcg ctcgtccacg cctatggtgc ggccttcgac aatcccgatc tggtggtggc ctgcgtcgtg ggcgacggag aagcggaaac cggcgcgctt gcaacttcgt ggcactccaa caaattcctc aatccggcgc gcgatggtgc ggttctgccg atcctgcatc tcaatggcta caagatcgcc aaccccaccg tgctggcccg cctttcggat gatgatctgg acaatctttt ccgtggctac ggttatgagc ctttctttgt tgaaggcagc gagcctgccg acatgcacca gaagatggcc gcaacactgg acacgatttt ccagcgcatt caggacatca agaaaaatgc cgatgtgcac tcgcccgagc gcccgcgctg gccgatgatt attctcagaa gcccgaaggg ctggaccggc ccaaaaaccg tggacggtct ggttgttgaa aactattggc gcgcccatca ggtgccggtt gccaattgcc gcgaaaacga tgcccatcgc aaaatcctcg aagactggat gaagagttac gacccgtccg atctgttcga cgaaaaaggg gcgctgaagc ctgaattgcg ggcgctggcg ccaaagggtg aagcgcgcat aggggccaat ccgcatgcca atggcgggct cttgcgtaaa gagcttcaca tgccggattt ccgccaatat gcggtcaatg tcaccgaacc gggagcgata gaagcgcaat caaccaaaat attgggcgat tttctgcgcg atgtgatgaa actgaacgag acggaaaaga acttccgcat cttcggcccc gacgaaaccg cctccaacag gctcggcagc gtattagagg cgacgaaccg ggtctggatg gccgaaacgc tggacatgga tgaccacctc gccgccgacg ggcgtgtaat ggaggttctc agcgaacatc tctgccaggg ctggcttgaa ggctatcttc tcagtggccg gcacggcttc ttttcctgct acgaagcctt catccacatt atcgattcca tgttcaacca acatgccaaa tggttgcagg ttgcgcgcga actggaatgg cgcaagccca tctcatcgct caattatctg ctgacctccc atgtctggcg gcaggaccat aacggcttct cccatcagga tcccggcttc gtcgatctcg tcgccaacaa aagcgccgat atcgtgcgtg tctatttccc gccggatgcc aacacccttt tgtgggtggg agatcactgc ctgaaaacct ggaaccgcgt gaatgtcatc gtggcgggca agcagccgga accgcaatgg ctgaccatgg cggaggctga gaaacattgc gaagcaggtc tcggcatatg ggaatgggcg ggcacggaag acgggctgga gccggatatc gtcatggcct gcgcgggcga tgtgccgacc atggaaacgc ttgccgcggt tgatcttctg cgccagtccc tgccgcatct gcgcattcgc gtggtgaatg tggttgacct catggtgctg caatcgccgc atcagcaccc gcatggcatt tccgatgagg aattcgaccg gatgttcacc accaacaggc ccgtcatctt cgcctatcac ggctatcctt atctcatcca tcgactggtc tataagcgca ccaaccactc caatttccac gttcgtggtt ttatcgagca gggaacgacc acaacgcctt tcgacatgac cgtgctgaac gagctggatc gtttccacct cgcgatggaa gccgtcgagc gcctgccgct gggcgaaagc gttgccaaac ccctgatcga caatttcacg gagaaacttg cgctgcataa ggactatatc cgacagcatg gtgaggacat gccggaaatc cgcgactgga aatggacatg gccgcgataa

Details for BrsuA.18174.a.A1.GE33972

HARVESTED ON:	11/21/2011
SEQUENCED ON:	11/29/2011
EXPECTED MW:	91kDa
OBSERVED MW:	82kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	81
PERCENT COVERAGE:	46

Validated AA Sequence

Validated NT Sequence

ttntttactt taagaaggag anataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atggtgccag caaaagggcc tctcacaccg cagcagcttt cactcatcaa ccgttactgg cgcgcggcga attatctttc cgtcggccag atttatctga tgaaaaatcc cctgttgcgc gaaccgctcc agcctgagca catcaagccg cggcttctcg gccattgggg cacgacaccc ggcctcaatt tcatctatgc gcatctcaac cgcattattc agcagcgcaa cgccaatgtg atctatattt gcggccccgg ccatggcggg ccgggcatgg tggccaacac ctatctggag ggcacctatt ccgaaatcta tcccgcaatc agcgaagatg aagcgggcat ggaaaggctc ttccgccagt tttccttccc cggcggaata ccaagccatg ccgcgccgga aacaccgggc tctatccacg aagggggcga actgggttat gcgctcgtcc acgcctatgg tgcggccttc gacaatcccg atctggtggt ggcctgcgtc gtgggcgacg gagaagcgga aaccggcgcg cttgcaactt cgtggcactc caacaaattc ctcaatccgg cgcgcgatgg tgcggttctg ccgatcctgc atctcaatgg ctacnagatc gccnacccca ccgtgctggc ccgcctttcg gatgatgatc tggacaatct tttccgtggc tacngttatg agcctttctt tgttgaaggc agcgagcctg ccgacatgca ccagaanatg gccgcaacac tggacacgat tttccngcgc attcnggaca tcnagaaaaa tgncgatgtg cactcgcccg agcgcccncn ctggncgatg attattctcn naagcnnnaa ggnctggann ngnccnnaaa nnntggnctg gttgttgaaa annantggcn nnccntcnnn gcnnnnncnn nnccgaaacg atgccnnncn caaancnncn aannctggnn gannnnntac naccgnncnn nnnnnnnnan naaaanngnn nnnnnnnctn nnntnnnnnn ctgggnnnnn ngnnnnannn nnnnnnnnng g

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVPAKGPLT PQQLSLINRY WRAANYLSVG QIYLMKNPLL REPLQPEHIK PRLLGHWGTT PGLNFIYAHL NRIIQQRNAN VIYICGPGHG GPGMVANTYL EGTYSEIYPA ISEDEAGMER LFRQFSFPGG IPSHAAPETP GSIHEGGELG YALVHAYGAA FDNPDLVVAC VVGDGEAETG ALATSWHSNK FLNPARDGAV LPILHLNGYK IANPTVLARL SDDDLDNLFR GYGYEPFFVE GSEPADMHQK MAATLDTIFQ RIQDIKKNAD VHSPERPRWP MIILRSPKGW TGPKTVDGLV VENYWRAHQV PVANCRENDA HRKILEDWMK SYDPSDLFDE KGALKPELRA LAPKGEARIG ANPHANGGLL RKELHMPDFR QYAVNVTEPG AIEAQSTKIL GDFLRDVMKL NETEKNFRIF GPDETASNRL GSVLEATNRV WMAETLDMDD HLAADGRVME VLSEHLCQGW LEGYLLSGRH GFFSCYEAFI HIIDSMFNQH AKWLQVAREL EWRKPISSLN YLLTSHVWRQ DHNGFSHQDP GFVDLVANKS ADIVRVYFPP DANTLLWVGD HCLKTWNRVN VIVAGKQPEP QWLTMAEAEK HCEAGLGIWE WAGTEDGLEP DIVMACAGDV PTMETLAAVD LLRQSLPHLR IRVVNVVDLM VLQSPHQHPH GISDEEFDRM FTTNRPVIFA YHGYPYLIHR LVYKRTNHSN FHVRGFIEQG TTTTPFDMTV LNELDRFHLA MEAVERLPLG ESVAKPLIDN FTEKLALHKD YIRQHGEDMP EIRDWKWTWP R

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgccagca aaagggcctc tcacaccgca gcagctttca ctcatcaacc gttactggcg cgcggcgaat tatctttccg tcggccagat ttatctgatg aaaaatcccc tgttgcgcga accgctccag cctgagcaca tcaagccgcg gcttctcggc cattggggca cgacacccgg cctcaatttc atctatgcgc atctcaaccg cattattcag cagcgcaacg ccaatgtgat ctatatttgc ggccccggcc atggcgggcc gggcatggtg gccaacacct atctggaggg cacctattcc gaaatctatc ccgcaatcag cgaagatgaa gcgggcatgg aaaggctctt ccgccagttt tccttccccg gcggaatacc aagccatgcc gcgccggaaa caccgggctc tatccacgaa gggggcgaac tgggttatgc gctcgtccac gcctatggtg cggccttcga caatcccgat ctggtggtgg cctgcgtcgt gggcgacgga gaagcggaaa ccggcgcgct tgcaacttcg tggcactcca acaaattcct caatccggcg cgcgatggtg cggttctgcc gatcctgcat ctcaatggct acaagatcgc caaccccacc gtgctggccc gcctttcgga tgatgatctg gacaatcttt tccgtggcta cggttatgag cctttctttg ttgaaggcag cgagcctgcc gacatgcacc agaagatggc cgcaacactg gacacgattt tccagcgcat tcaggacatc aagaaaaatg ccgatgtgca ctcgcccgag cgcccgcgct ggccgatgat tattctcaga agcccgaagg gctggaccgg cccaaaaacc gtggacggtc tggttgttga aaactattgg cgcgcccatc aggtgccggt tgccaattgc cgcgaaaacg atgcccatcg caaaatcctc gaagactgga tgaagagtta cgacccgtcc gatctgttcg acgaaaaagg ggcgctgaag cctgaattgc gggcgctggc gccaaagggt gaagcgcgca taggggccaa tccgcatgcc aatggcgggc tcttgcgtaa agagcttcac atgccggatt tccgccaata tgcggtcaat gtcaccgaac cgggagcgat agaagcgcaa tcaaccaaaa tattgggcga ttttctgcgc gatgtgatga aactgaacga gacggaaaag aacttccgca tcttcggccc cgacgaaacc gcctccaaca ggctcggcag cgtattagag gcgacgaacc gggtctggat ggccgaaacg ctggacatgg atgaccacct cgccgccgac gggcgtgtaa tggaggttct cagcgaacat ctctgccagg gctggcttga aggctatctt ctcagtggcc ggcacggctt cttttcctgc tacgaagcct tcatccacat tatcgattcc atgttcaacc aacatgccaa atggttgcag gttgcgcgcg aactggaatg gcgcaagccc atctcatcgc tcaattatct gctgacctcc catgtctggc ggcaggacca taacggcttc tcccatcagg atcccggctt cgtcgatctc gtcgccaaca aaagcgccga tatcgtgcgt gtctatttcc cgccggatgc caacaccctt ttgtgggtgg gagatcactg cctgaaaacc tggaaccgcg tgaatgtcat cgtggcgggc aagcagccgg aaccgcaatg gctgaccatg gcggaggctg agaaacattg cgaagcaggt ctcggcatat gggaatgggc gggcacggaa gacgggctgg agccggatat cgtcatggcc tgcgcgggcg atgtgccgac catggaaacg cttgccgcgg ttgatcttct gcgccagtcc ctgccgcatc tgcgcattcg cgtggtgaat gtggttgacc tcatggtgct gcaatcgccg catcagcacc cgcatggcat ttccgatgag gaattcgacc ggatgttcac caccaacagg cccgtcatct tcgcctatca cggctatcct tatctcatcc atcgactggt ctataagcgc accaaccact ccaatttcca cgttcgtggt tttatcgagc agggaacgac cacaacgcct ttcgacatga ccgtgctgaa cgagctggat cgtttccacc tcgcgatgga agccgtcgag cgcctgccgc tgggcgaaag cgttgccaaa cccctgatcg acaatttcac ggagaaactt gcgctgcata aggactatat ccgacagcat ggtgaggaca tgccggaaat ccgcgactgg aaatggacat ggccgcgata aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a