BrsuA.18170.a

UPF0261 protein BRA1168

CENTER ID: BrsuA.18170.a
ORGANISM: Brucella suis 1330
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuA.18170.a.A1.GE33944 full length 1 418
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|204722.5.peg.3399
RefSeq: NP_700321.1
UniProt: Q8FUQ2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTAHTNSPRI LVIGTGDTKS DELLFMADVI ERAGGSPVMI DVSILGNPPY EPAYSKHDVA EAAGTTVQAI IDSGDEHSAM ALMAEGATAL VRGLSQRGQV DGMIALGGSL GTDLALDIAA ILPLGVPKFI VSTIAYSHLL PPERIAPDLM MILWAGGLYG LNPICRSVLS QACGAVVGAA KLVEKPSAEK PLIGMTSLGS SCLKYMRFLK PELEKRGYDV AIFHATGMGG RAYEAVAAQK GFVAVFDFCI QEVTNAESGS VVTSGPDRME NAERAGIPQI IAPGAVDMVD MPAWQNVPEQ FRDRPYHAHN RLIASITVSP EQRRAVARVV AAKLERAAAP VAFILPTGGV QERDRNGEPL HEPEALGAFL DEMRGAVSGT ITFEEVDAHI NAPEFASRAL AVFDRWVAEG IVVKGNVA
NT Sequence
atgactgcgc ataccaattc cccccgcatt ctggtgatcg gcacgggcga caccaagagt gatgaactgc ttttcatggc ggatgtcatc gaaagggctg gcggctcacc ggtgatgatc gatgtcagca ttctgggcaa tccgccttat gagccggctt attccaagca tgatgtggct gaagccgccg gaacgacggt tcaggcgatt atcgacagcg gcgatgaaca tagcgccatg gccctgatgg ccgaaggggc gacggctctc gttcgtggcc tttcgcaacg cggccaggtc gatggcatga ttgcgcttgg cggttcgctt ggcacggacc ttgcgctgga tattgcggcg atcctgccgc ttggcgtgcc gaaattcatc gtttcgacca ttgcctattc acatcttctg ccgccggagc gcatcgcgcc cgacctcatg atgatcctat gggccggggg gctttacggc ctcaacccga tctgccgctc cgttctgtcg caagcctgcg gcgcggtggt gggcgcggcg aaattggtgg agaagcccag tgctgaaaag ccgctgatcg gcatgacgtc gcttggttct tcctgcctca aatatatgcg ctttctaaaa ccggagctgg aaaaacgcgg ctatgatgtt gcgatatttc acgccacggg catgggtggc cgcgcctatg aggcggttgc ggcgcagaag ggttttgtcg ccgttttcga tttctgcata caggaagtga ccaatgccga gagcggctct gtggtcacgt ccgggccgga ccgtatggaa aatgcggagc gggcaggcat accccagatc atcgcgccgg gggcggtgga tatggtggat atgcccgcct ggcagaatgt gccggaacag ttccgggacc ggccctatca cgcccataac cgtctgattg catccatcac cgtttcgccg gaacagcgcc gcgcagtggc ccgcgtggtg gcagccaagc tggagcgcgc cgcagcaccg gttgccttca tcctgccgac cggcggcgtg caggaaaggg atcgcaacgg cgaaccgctg catgagccgg aagcgctcgg tgcctttctt gacgagatgc ggggtgcggt ttccgggaca atcacttttg aggaagtgga tgcgcatatc aatgcgccgg aatttgcatc gcgcgccctt gcggtcttcg accgctgggt cgcggaagga attgtagtga aaggcaatgt tgcatga
Details for BrsuA.18170.a.A1.GE33944
HARVESTED ON: 11/21/2011
SEQUENCED ON: 11/29/2011
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 74
PERCENT COVERAGE: 85
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTAHTNSPR ILVIGTGDTK SDELLFMADV IERAGGSPVM IDVSILGNPP YEPAYSKHDV AEAAGTTVQA IIDSGDEHSA MALMAEGATA LVRGLSQRGQ VDGMIALGGS LGTDLALDIA AILPLGVPKF IVSTIAYSHL LPPERIAPDL MMILWAGGLY GLXPICRSVL SXACGAVVGA AKLVEKPSAE KPLIGMTSLG SSCLKYMRFL KPELEKRGYD VAIFHATGMG GRAYXXVXAX XGFVAVFXFC IXEXTXAXXG SVVTSXPDXX EXXXGXXTXX XXXGXXXXXX PXGXXXXXXX XPXXXXXXXX XXXVXXXXPX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXX
Validated NT Sequence
attttnnnnn ntttangang gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgactg cgcataccaa ttccccccgc attctggtga tcggcacggg cgacaccaag agtgatgaac tgcttttcat ggcggatgtc atcgaaaggg ctggcggctc accggtgatg atcgatgtca gcattctggg caatccgcct tatgagccgg cttattccaa gcatgatgtg gctgaagccg ccggaacgac ggttcaggcg attatcgaca gcggcgatga acatagcgcc atggccctga tggccgaagg ggcgacggct ctcgttcgtg gcctttcgca acgcggccag gtcgatggca tgattgcgct tggcggttcg cttggcacgg accttgcgct ggatattgcg gcgatcctgc cgcttggcgt gccgaaattc atcgtttcga ccattgccta ttcacatctt ctgccgccgg agcgcatcgc gcccgacctc atgatgatcc tatgggccgg ggggctttac ggcctcnacc cgatctgccg ctccgttctg tcgcangcct gcggcgcggt ggtgggcgcg gcgaaattgg tggagaagcc cagtgctgaa aagccgctga tcggcatgac gtcgcttggt tcttcctgcc tcaaatatat gcgctttcta aaaccggagc tggaaaaacg cggctatgat gttgcgatat ttcacgccac gggcatgggt ggccgcgcct atnaggnggt tgnggcgcan aanggttttg tcgccgtttt cnatttctgc atacnggaan ngaccnatgc cnagagnggc tctgtggtca cgtccnggcc ggaccnnnng gaaannnnna nngggcnnnn tacccnnanc ntcnnncngg gnnnngnnnn ggnngnnnng ccnnctggnn nannnnnnnn nngtnnngna cnnccnnntc nnnncnnnnc nnnnnntnnn nnnnnnnncg ttncnnnnnn nnncccnnnn nnnnnnnnnn nnnnggnanc nnnnngnnnn nnnnncnnnn nntnnnnnnn nnnnnnnnnn nnnnnaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntnnnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTAHTNSPR ILVIGTGDTK SDELLFMADV IERAGGSPVM IDVSILGNPP YEPAYSKHDV AEAAGTTVQA IIDSGDEHSA MALMAEGATA LVRGLSQRGQ VDGMIALGGS LGTDLALDIA AILPLGVPKF IVSTIAYSHL LPPERIAPDL MMILWAGGLY GLNPICRSVL SQACGAVVGA AKLVEKPSAE KPLIGMTSLG SSCLKYMRFL KPELEKRGYD VAIFHATGMG GRAYEAVAAQ KGFVAVFDFC IQEVTNAESG SVVTSGPDRM ENAERAGIPQ IIAPGAVDMV DMPAWQNVPE QFRDRPYHAH NRLIASITVS PEQRRAVARV VAAKLERAAA PVAFILPTGG VQERDRNGEP LHEPEALGAF LDEMRGAVSG TITFEEVDAH INAPEFASRA LAVFDRWVAE GIVVKGNVA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gactgcgcat accaattccc cccgcattct ggtgatcggc acgggcgaca ccaagagtga tgaactgctt ttcatggcgg atgtcatcga aagggctggc ggctcaccgg tgatgatcga tgtcagcatt ctgggcaatc cgccttatga gccggcttat tccaagcatg atgtggctga agccgccgga acgacggttc aggcgattat cgacagcggc gatgaacata gcgccatggc cctgatggcc gaaggggcga cggctctcgt tcgtggcctt tcgcaacgcg gccaggtcga tggcatgatt gcgcttggcg gttcgcttgg cacggacctt gcgctggata ttgcggcgat cctgccgctt ggcgtgccga aattcatcgt ttcgaccatt gcctattcac atcttctgcc gccggagcgc atcgcgcccg acctcatgat gatcctatgg gccggggggc tttacggcct caacccgatc tgccgctccg ttctgtcgca agcctgcggc gcggtggtgg gcgcggcgaa attggtggag aagcccagtg ctgaaaagcc gctgatcggc atgacgtcgc ttggttcttc ctgcctcaaa tatatgcgct ttctaaaacc ggagctggaa aaacgcggct atgatgttgc gatatttcac gccacgggca tgggtggccg cgcctatgag gcggttgcgg cgcagaaggg ttttgtcgcc gttttcgatt tctgcataca ggaagtgacc aatgccgaga gcggctctgt ggtcacgtcc gggccggacc gtatggaaaa tgcggagcgg gcaggcatac cccagatcat cgcgccgggg gcggtggata tggtggatat gcccgcctgg cagaatgtgc cggaacagtt ccgggaccgg ccctatcacg cccataaccg tctgattgca tccatcaccg tttcgccgga acagcgccgc gcagtggccc gcgtggtggc agccaagctg gagcgcgccg cagcaccggt tgccttcatc ctgccgaccg gcggcgtgca ggaaagggat cgcaacggcg aaccgctgca tgagccggaa gcgctcggtg cctttcttga cgagatgcgg ggtgcggttt ccgggacaat cacttttgag gaagtggatg cgcatatcaa tgcgccggaa tttgcatcgc gcgcccttgc ggtcttcgac cgctgggtcg cggaaggaat tgtagtgaaa ggcaatgttg cataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa