BrsuA.18159.a
CAIB/BAIF family protein
| CENTER ID: | BrsuA.18159.a |
| ORGANISM: | Brucella suis 1330 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrsuA.18159.a.A1.GE33937 | full length | 1 | 381 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrsuA.18159.a.A1.PW34608 | full length | 1 | 381 |
Structures
4ED9
DEPOSITED: 3/27/2012
DETERMINATION: XRay
CLONE: BrsuA.18159.a.A1.GE33937
PROTEIN: BrsuA.18159.a.A1.PW34608
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_128463 |
| PATRIC ID: | fig|204722.5.peg.1105 |
| RefSeq: | NP_698089.1 |
| UniProt: | A0A0H3G7M3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQNTPLDGLK VVELARILAG PWVGQTLCDL GADVIKVESP EGDDTRTWGP PFIDVEGERS AAYFHACNRG KRSITADFRT EEGRELVRRL VAEADVVIEN FKLGGLDKYG LDYESLKAIN PQLIYCSITG FGHTGPYAER AGYDFMIQGM GGIMDLTGEP DREPQKIGVA FADIFTGLYS VIAIQSALIM RARTGKGQHI DMALFDCMSG VLANQAMNYL ASGKSPKRMG NAHPNIAPYQ TLSVSDGYFI IACGNDGQFG KLSTLLGIGE LAKDERFATN SARVANRAAL TALLEERTKQ WKRDDLLAEL AKIGVPAGPI NTVADVFADP QFKARGMKID PQGVPGLRTP IRFSDADLKL DSRSPKLNEH GAAIRAELDK G
NT Sequence
atgcagaaca caccgcttga cggtctcaag gtggtcgaac tggcgcgtat tctggccggg ccatgggttg gccagacgct ctgcgatctt ggtgccgatg tgatcaaggt cgaaagccct gagggtgatg acacacgcac ctgggggccg cctttcattg atgtcgaagg cgaacggtcg gctgcgtatt tccatgcctg caatcgcggc aaacggtcca ttacagccga ttttcgcacc gaggagggca gggagctcgt gcgccgtctg gtggcggagg ctgacgtcgt catcgagaat ttcaagctgg gtgggctcga caaatacggt ctggattatg aaagcctgaa agcgataaat ccgcagctca tctattgctc aatcacgggg ttcggccata ccggccctta tgcggagcgt gccggatatg acttcatgat ccagggcatg ggcggcatca tggatttgac cggcgagcca gaccgcgagc cgcagaaaat cggtgtggcc tttgcggata tctttaccgg cctttatagc gtgattgcga tccagtcggc ccttatcatg cgcgcgcgta ccggcaaggg ccagcatatc gatatggcct tgtttgattg catgtcgggg gtgctggcca atcaggccat gaattacctt gcttccggca aatcgccaaa gcgcatgggg aacgcgcatc caaatatcgc gccttatcag acgctttctg tttcagatgg ttacttcatc atcgcctgtg gcaatgatgg gcaatttgga aagctttcta cgctgctggg tatcggtgaa ctggcaaagg acgagcgttt cgctaccaat tcggcacgcg tcgccaatcg tgcggccctt acggcgctat tggaggaacg cacaaaacag tggaagcgtg acgacctgct ggcagagctt gcgaaaatag gcgtacccgc cggcccgatc aatacggtgg ctgatgtctt cgccgatccg caattcaagg cgagagggat gaagatcgac ccgcagggtg ttccgggatt gcgcacgccg atccgcttct ctgatgctga tttaaaactc gacagccgct cgccgaaact aaatgaacac ggcgctgcga tccgcgccga actcgacaaa ggctga
Details for BrsuA.18159.a.A1.GE33937
| HARVESTED ON: | 11/21/2011 |
| SEQUENCED ON: | 11/29/2011 |
| EXPECTED MW: | 43kDa |
| OBSERVED MW: | 43kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 94 |
Validated AA Sequence
MQNTPLDGLK VVELARILAG PWVGQTLCDL GADVIKVESP EGDDTRTWGP PFIDVEGERS AAYFHACNRG KRSITADFRT EEGRELVRRL VAEADVVIEN FKLGGLDKYG LDYESLKAIN PQLIYCSITG FGHTGPYAER AGYDFMIQGM GGIMDLTGEP DREPQKIGVA FADIFTGLYS VIAIQSALIM RARTGKGQHI DMALFDCMSG VLANQAMNYL ASGKSPKRMG NAHPNIAPYQ TLSVSDGYFI IACGNDGQFG KLSTLLGIGE LAKDERFATN SARVANRAAL TALLEERTKQ WKRDDLLAEL AKIGVPAGPI NTVADVFADP QFKARGMKID PQGVPGLRTP IRFSDADLKL DSRSPKLNEH GAAIRAELD
Validated NT Sequence
tgtcgagttc ggcgcggatc gcagcgccgt gttcatttag tttcggcgag cggctgtcga gttttaaatc agcatcagag aagcggatcg gcgtgcgcaa tcccggaaca ccctgcgggt cgatcttcat ccctctcgcc ttgaattgcg gatcggcgaa gacatcagcc accgtattga tcgggccggc gggtacgcct attttcgcaa gctctgccag caggtcgtca cgcttccact gttttgtgcg ttcctccaat agcgccgtaa gggccgcacg attggcgacg cgtgccgaat tggtagcgaa acgctcgtcc tttgccagtt caccgatacc cagcagcgta gaaagctttc caaattgccc atcattgcca caggcgatga tgaagtaacc atctgaaaca gaaagcgtct gataaggcgc gatatttgga tgcgcgttcc ccatgcgctt tggcgatttg ccggaagcaa ggtaattcat ggcctgattg gccagcaccc ccgacatgca atcaaacaag gccatatcga tatgctggcc cttgccggta cgcgcgcgca tgataagggc cgactggatc gcaatcacgc tataaaggcc ggtaaagata tccgcaaagg ccacaccgat tttctgcggc tcgcggtctg gctcgccggt caaatccatg atgccgccca tgccctggat catgaagtca tatccggcac gctccgcata agggccggta tggccgaacc ccgtgattga gcaatagatg agctgcggat ttatcgcttt caggctttca taatccagac cgtatttgtc gagcccaccc agcttgaaat tctcgatgac gacgtcagcc tccgccacca gacggcgcac gagctccctg ccctcctcgg tgcgaaaatc ggctgtaatg gaccgtttgc cgcgattgca ggcatggaaa tacgcagccg accgttcgcc ttcgacatca atgaaaggcg gcccccaggt gcgtgtgtca tcaccctcag ggctttcgac cttgatcaca tcggcaccaa gatcgcagag cgtctggcca acccatggcc cggccagaat acgcgccagt tcgaccacct tgagaccgtc aagcggtgtg ttctgcatcg aaccaggacc ctgggtctga gcttccag
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQNTPLDGL KVVELARILA GPWVGQTLCD LGADVIKVES PEGDDTRTWG PPFIDVEGER SAAYFHACNR GKRSITADFR TEEGRELVRR LVAEADVVIE NFKLGGLDKY GLDYESLKAI NPQLIYCSIT GFGHTGPYAE RAGYDFMIQG MGGIMDLTGE PDREPQKIGV AFADIFTGLY SVIAIQSALI MRARTGKGQH IDMALFDCMS GVLANQAMNY LASGKSPKRM GNAHPNIAPY QTLSVSDGYF IIACGNDGQF GKLSTLLGIG ELAKDERFAT NSARVANRAA LTALLEERTK QWKRDDLLAE LAKIGVPAGP INTVADVFAD PQFKARGMKI DPQGVPGLRT PIRFSDADLK LDSRSPKLNE HGAAIRAELD KG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcagaacaca ccgcttgacg gtctcaaggt ggtcgaactg gcgcgtattc tggccgggcc atgggttggc cagacgctct gcgatcttgg tgccgatgtg atcaaggtcg aaagccctga gggtgatgac acacgcacct gggggccgcc tttcattgat gtcgaaggcg aacggtcggc tgcgtatttc catgcctgca atcgcggcaa acggtccatt acagccgatt ttcgcaccga ggagggcagg gagctcgtgc gccgtctggt ggcggaggct gacgtcgtca tcgagaattt caagctgggt gggctcgaca aatacggtct ggattatgaa agcctgaaag cgataaatcc gcagctcatc tattgctcaa tcacggggtt cggccatacc ggcccttatg cggagcgtgc cggatatgac ttcatgatcc agggcatggg cggcatcatg gatttgaccg gcgagccaga ccgcgagccg cagaaaatcg gtgtggcctt tgcggatatc tttaccggcc tttatagcgt gattgcgatc cagtcggccc ttatcatgcg cgcgcgtacc ggcaagggcc agcatatcga tatggccttg tttgattgca tgtcgggggt gctggccaat caggccatga attaccttgc ttccggcaaa tcgccaaagc gcatggggaa cgcgcatcca aatatcgcgc cttatcagac gctttctgtt tcagatggtt acttcatcat cgcctgtggc aatgatgggc aatttggaaa gctttctacg ctgctgggta tcggtgaact ggcaaaggac gagcgtttcg ctaccaattc ggcacgcgtc gccaatcgtg cggcccttac ggcgctattg gaggaacgca caaaacagtg gaagcgtgac gacctgctgg cagagcttgc gaaaataggc gtacccgccg gcccgatcaa tacggtggct gatgtcttcg ccgatccgca attcaaggcg agagggatga agatcgaccc gcagggtgtt ccgggattgc gcacgccgat ccgcttctct gatgctgatt taaaactcga cagccgctcg ccgaaactaa atgaacacgg cgctgcgatc cgcgccgaac tcgacaaagg ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for BrsuA.18159.a.A1.PW34608
| PURIFICATION DATe: | 1/29/2012 |
| CONCENTRATION: | 40.63mg/ml |
| OBSERVED MW: | 42kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 15 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 94 |
Protocol Notes
notes unavailable
Validated AA Sequence
MQNTPLDGLK VVELARILAG PWVGQTLCDL GADVIKVESP EGDDTRTWGP PFIDVEGERS AAYFHACNRG KRSITADFRT EEGRELVRRL VAEADVVIEN FKLGGLDKYG LDYESLKAIN PQLIYCSITG FGHTGPYAER AGYDFMIQGM GGIMDLTGEP DREPQKIGVA FADIFTGLYS VIAIQSALIM RARTGKGQHI DMALFDCMSG VLANQAMNYL ASGKSPKRMG NAHPNIAPYQ TLSVSDGYFI IACGNDGQFG KLSTLLGIGE LAKDERFATN SARVANRAAL TALLEERTKQ WKRDDLLAEL AKIGVPAGPI NTVADVFADP QFKARGMKID PQGVPGLRTP IRFSDADLKL DSRSPKLNEH GAAIRAELD
Validated NT Sequence
tgtcgagttc ggcgcggatc gcagcgccgt gttcatttag tttcggcgag cggctgtcga gttttaaatc agcatcagag aagcggatcg gcgtgcgcaa tcccggaaca ccctgcgggt cgatcttcat ccctctcgcc ttgaattgcg gatcggcgaa gacatcagcc accgtattga tcgggccggc gggtacgcct attttcgcaa gctctgccag caggtcgtca cgcttccact gttttgtgcg ttcctccaat agcgccgtaa gggccgcacg attggcgacg cgtgccgaat tggtagcgaa acgctcgtcc tttgccagtt caccgatacc cagcagcgta gaaagctttc caaattgccc atcattgcca caggcgatga tgaagtaacc atctgaaaca gaaagcgtct gataaggcgc gatatttgga tgcgcgttcc ccatgcgctt tggcgatttg ccggaagcaa ggtaattcat ggcctgattg gccagcaccc ccgacatgca atcaaacaag gccatatcga tatgctggcc cttgccggta cgcgcgcgca tgataagggc cgactggatc gcaatcacgc tataaaggcc ggtaaagata tccgcaaagg ccacaccgat tttctgcggc tcgcggtctg gctcgccggt caaatccatg atgccgccca tgccctggat catgaagtca tatccggcac gctccgcata agggccggta tggccgaacc ccgtgattga gcaatagatg agctgcggat ttatcgcttt caggctttca taatccagac cgtatttgtc gagcccaccc agcttgaaat tctcgatgac gacgtcagcc tccgccacca gacggcgcac gagctccctg ccctcctcgg tgcgaaaatc ggctgtaatg gaccgtttgc cgcgattgca ggcatggaaa tacgcagccg accgttcgcc ttcgacatca atgaaaggcg gcccccaggt gcgtgtgtca tcaccctcag ggctttcgac cttgatcaca tcggcaccaa gatcgcagag cgtctggcca acccatggcc cggccagaat acgcgccagt tcgaccacct tgagaccgtc aagcggtgtg ttctgcatcg aaccaggacc ctgggtctga gcttccag
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQNTPLDGL KVVELARILA GPWVGQTLCD LGADVIKVES PEGDDTRTWG PPFIDVEGER SAAYFHACNR GKRSITADFR TEEGRELVRR LVAEADVVIE NFKLGGLDKY GLDYESLKAI NPQLIYCSIT GFGHTGPYAE RAGYDFMIQG MGGIMDLTGE PDREPQKIGV AFADIFTGLY SVIAIQSALI MRARTGKGQH IDMALFDCMS GVLANQAMNY LASGKSPKRM GNAHPNIAPY QTLSVSDGYF IIACGNDGQF GKLSTLLGIG ELAKDERFAT NSARVANRAA LTALLEERTK QWKRDDLLAE LAKIGVPAGP INTVADVFAD PQFKARGMKI DPQGVPGLRT PIRFSDADLK LDSRSPKLNE HGAAIRAELD KG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCAGAACACA CCGCTTGACG GTCTCAAGGT GGTCGAACTG GCGCGTATTC TGGCCGGGCC ATGGGTTGGC CAGACGCTCT GCGATCTTGG TGCCGATGTG ATCAAGGTCG AAAGCCCTGA GGGTGATGAC ACACGCACCT GGGGGCCGCC TTTCATTGAT GTCGAAGGCG AACGGTCGGC TGCGTATTTC CATGCCTGCA ATCGCGGCAA ACGGTCCATT ACAGCCGATT TTCGCACCGA GGAGGGCAGG GAGCTCGTGC GCCGTCTGGT GGCGGAGGCT GACGTCGTCA TCGAGAATTT CAAGCTGGGT GGGCTCGACA AATACGGTCT GGATTATGAA AGCCTGAAAG CGATAAATCC GCAGCTCATC TATTGCTCAA TCACGGGGTT CGGCCATACC GGCCCTTATG CGGAGCGTGC CGGATATGAC TTCATGATCC AGGGCATGGG CGGCATCATG GATTTGACCG GCGAGCCAGA CCGCGAGCCG CAGAAAATCG GTGTGGCCTT TGCGGATATC TTTACCGGCC TTTATAGCGT GATTGCGATC CAGTCGGCCC TTATCATGCG CGCGCGTACC GGCAAGGGCC AGCATATCGA TATGGCCTTG TTTGATTGCA TGTCGGGGGT GCTGGCCAAT CAGGCCATGA ATTACCTTGC TTCCGGCAAA TCGCCAAAGC GCATGGGGAA CGCGCATCCA AATATCGCGC CTTATCAGAC GCTTTCTGTT TCAGATGGTT ACTTCATCAT CGCCTGTGGC AATGATGGGC AATTTGGAAA GCTTTCTACG CTGCTGGGTA TCGGTGAACT GGCAAAGGAC GAGCGTTTCG CTACCAATTC GGCACGCGTC GCCAATCGTG CGGCCCTTAC GGCGCTATTG GAGGAACGCA CAAAACAGTG GAAGCGTGAC GACCTGCTGG CAGAGCTTGC GAAAATAGGC GTACCCGCCG GCCCGATCAA TACGGTGGCT GATGTCTTCG CCGATCCGCA ATTCAAGGCG AGAGGGATGA AGATCGACCC GCAGGGTGTT CCGGGATTGC GCACGCCGAT CCGCTTCTCT GATGCTGATT TAAAACTCGA CAGCCGCTCG CCGAAACTAA ATGAACACGG CGCTGCGATC CGCGCCGAAC TCGACAAAGG CTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA