SSGCID: Target Page

BrsuA.18150.a

Glutamate synthase, large subunit

CENTER ID:	BrsuA.18150.a
ORGANISM:	Brucella suis 1330
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuA.18150.a.A2.GE33817	domain1		1	795
BrsuA.18150.a.A3.GE33814	domain2		796	1583

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|204722.5.peg.2274
RefSeq:	NP_699261.1
UniProt:	A0A0H3G9F3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTHLTPSVSN VVSHNVQNEA VKAATTASTR TTRKTKAAGV PPAQGLYDPR NEHDACGVGF IAHMKGRKSH VVVENGLKML ENLTHRGAVG ADPLMGDGAG ILVQIPDRFF REEMARQGID LPQPGHYGVG YIFMPRDPDL RAHAEEIVRE VIAAEGQTFI GFREVPVDNS SLSKAPHIAA TEPFHMQVFI GRSPMLETDD DFERRLFVLR KVISNRIYQE NDGDDKGFYV VSMSARTVVY KGMFLAYQVG AYYLDLKDPR FESAVALVHQ RFSTNTFPSW RLAHPYRMVA HNGEINTLRG NVNWMAARQA SVDSELFGND ISKLWPISYE GQSDTACFDN ALEFLHQGGY SLAHAMMMLI PEAWSGNKLM SDERRAFYEY HAALMEPWDG PAAVAFTDGR QIGAMLDRNG LRPARYIVTE DDFVILASEA GVLPVDEKKI VRKWRLQPGR MLLIDMEEGR IISDEEIKSQ IAQKHPYKQW LANTQLILED LNPVEPRALH KDVSLLDRQQ AFGYTQEDTK FLMAPMATTG QEAIGSMGTD TPISAMSDKS KLLYTYFKQN FAQVTNPPID PIREELVMSL VSFIGPRPNI FDLVGTSRRK RLEVRQPILT NGDLEKIRSI GHTEDRFDTK TLDITYNTGE GATGMHGAIE RLCDRAEAAV HGGYNIVILS DRQVGPDRIP IPALLATAAV HHHLIRKGLR TSVGLVVESG EPREVHHFAC LAGYGAEAIN PYLAFDTLLD MHRRREFPPE VDEHEVVKRY IKSIGKGILK VMSKMGISTY QSYCGAQIFD AVGVRTDFVD KFFFGTATTI EGVGLDEIAE ETVRRHTDAF GNDPVLLNAL EVGGEYAYRM RGEAHMWSPD AVAKLQHAVR TSNPDTFTEY TRMLDAKSSQ AKTIRGLFNI RFAEERGLKP VSIDEVEPAT DIVKRFSTGA MSFGSISREA HTTLARAMNA IGGKSNTGEG GEEPDRFYPL PDGTANPERS AIKQVASGRF GVTTEYLVNS DLIQIKVAQG AKPGEGGQLP GHKVDATIAK TRHSTPGVGL ISPPPHHDIY SIEDLAQLIY DLKNVNPAAD ISVKLVSEVG VGTVAAGVAK ARADHITVSG YDGGTGASPL TSLKHAGSPW EIGLAETHQT LVLNGLRSRV ALQVDGGLRT GRDVVIGALL GADEFGFSTA PLIAAGCIMM RKCHLNTCPV GVATQDPVLR KRFKGTPEHV INFFFYLAEE VRALLAQMGF TRLEQIIGET ELLEKQAMID HWKAKGLDFS RIFYKPEAEK HEIYWTERQH HPIDDILDRK LIELAAPALE ERQPVKIDVE IKNVDRSAGA MLSGEVAKRF RHKGLPEDTI SVTLRGTAGQ SFGAFLARGI SFELIGDGND YIGKGLSGGR IVVRPPEDTR IVAEDSIIVG NTVLYGALEG ECYFRGVAGE RFAVRNSGAV TVVEGVGDHG CEYMTGGVVV VIGQTGRNFA AGMSGGVAYV LDEEGDFAQR CNMAMVELEP VPEEDDILEK LHHHGGDLMH KGRVDVSANM THHDEERLVQ LIANHLHYTG STRAKDILDN WESYRPKFVK VMPVEYRRAL EEMERMQIGV AAE

NT Sequence

atgacgcatt tgacgccatc tgtatcgaat gtggtttctc acaacgtaca gaacgaagcg gtcaaagctg cgacgacggc ttccacccga acgacgcgaa aaaccaaggc tgcgggtgtt ccgccggctc aaggccttta tgatccgcgc aacgagcatg atgcgtgcgg cgtcggcttc atcgcccata tgaagggcag gaaatcacat gtggtcgtgg aaaacggcct gaagatgctg gaaaacctca cccatcgtgg tgctgtcggc gccgatccgc tgatgggcga cggtgcgggc attctggtgc agattccaga tcgcttcttt cgcgaggaaa tggcgcgtca gggcatagac ctgccgcagc cgggtcacta tggcgtcggc tatattttca tgccgcgcga cccggatctg cgcgcccatg cggaagagat cgtcagggaa gtgatcgccg ccgaaggcca gaccttcatc ggcttccgcg aagtgccggt cgataattct tcgctttcca aggctccgca tatcgcggcc accgagccgt tccatatgca ggtcttcatc gggcgcagcc cgatgctgga aaccgacgac gatttcgagc gccgcctgtt cgtgctgcgc aaggtcatct ccaaccgcat ctatcaggag aatgacggcg acgataaggg tttctatgtc gtgtccatgt cggcccgcac ggtggtctat aagggcatgt tcctggccta tcaggtcggc gcttattatc tggacttgaa agacccccgc tttgaaagcg cggtggcgct ggtgcaccag cgtttttcca ccaacacctt tccgtcctgg cgtcttgccc acccctaccg catggtcgcc cataacggcg aaatcaacac gctgcgcggc aatgtcaact ggatggcggc gcgccaggct tcggtcgata gcgaactgtt cggcaacgat atttccaagc tctggccaat ttcctatgag ggccagtcgg atacggcctg tttcgacaat gcgctcgaat tcctgcatca gggcggctac agccttgcac atgccatgat gatgctgatc ccggaagcat ggtcgggcaa caagctgatg tcggatgagc gccgcgcgtt ctacgaatat catgcagcac tcatggagcc gtgggatggc cctgccgctg tcgccttcac cgatggccgc cagatcggcg cgatgctgga ccgcaacggc ctgcggcctg cacgctatat cgtgacggaa gacgatttcg tcattctggc ttcggaagca ggcgtcctgc cggtcgatga aaaaaagatc gtcaggaagt ggcgtcttca gccgggccgc atgctgctga tcgacatgga agaaggccgc atcatctccg atgaggaaat caagtcgcag atcgcgcaga agcaccctta caagcagtgg cttgccaata cgcagctcat tctggaagac ctcaacccgg tcgagccgcg tgcgctgcac aaggatgtga gcctgctcga ccgccagcag gccttcggct atacgcagga agataccaag ttcctgatgg cgccgatggc gaccacgggt caggaagcca tcggctccat gggcaccgat acgccaattt cggccatgtc cgacaagtcg aagctgcttt acacctattt caagcagaac ttcgcacagg tgacgaaccc gccgatcgat ccgatccgcg aggaactggt gatgagcctc gtctccttca ttggcccgcg tccaaacatc ttcgatcttg tcggcacctc gcgccgcaag cgtctggaag tgcgccagcc catcctgacc aatggcgatc tggaaaagat ccgttctatc ggccacacgg aagaccgctt cgataccaag acgctcgaca tcacctataa tacgggcgag ggcgcgaccg gtatgcatgg cgccatcgag cgtctgtgcg accgcgcgga agcagccgtt catggcggct ataacatcgt catcctctcc gaccgccagg tcgggccgga ccgtattccg atcccggccc tgctggcgac ggctgccgtg caccatcacc tgatccgcaa gggtcttcgc acctcggtcg gtcttgtggt ggaatcgggc gagccgcgcg aagtgcatca ttttgcctgc ctcgcaggct atggcgccga agcgatcaat ccctatctcg ccttcgacac gctgctcgac atgcatcgcc gccgcgaatt tccgccggaa gtcgacgagc atgaagtggt caagcgctac atcaagtcga ttggcaaggg catcctcaag gtcatgtcca agatgggtat ctcgacctat cagtcctatt gcggcgcgca gattttcgat gcggtcggcg tcaggacgga tttcgtggac aagttcttct tcggcaccgc cacgaccatc gaaggcgttg gcctcgacga gatcgcggaa gaaaccgtgc gccgtcacac cgatgctttc ggcaatgatc ccgttcttct gaacgcgctc gaagtcggcg gcgaatatgc ctatcgtatg cgcggcgagg cgcatatgtg gtcgccggat gcggtggcaa agctgcaaca tgcggtgcgc acgtccaatc cagacacgtt caccgaatat acgaggatgc tcgacgccaa gtcgtcgcag gccaagacca ttcgcggcct gttcaatatc cgctttgccg aggaacgtgg cctgaagccc gtttccatcg atgaggtgga gccggcaacg gatatcgtca agcggttctc aaccggcgcc atgtccttcg gctcgatctc cagagaggcg cataccacgc ttgcccgcgc gatgaacgcc attggcggca agtcgaacac cggcgagggc ggcgaggagc cggatcgttt ctacccgctg ccggatggca cggcgaaccc ggaacgctcg gcaatcaagc aggtggcctc gggccgtttc ggtgtgacga cggaatatct cgtcaattcc gatctgatcc agatcaaggt tgcgcagggc gcaaagcccg gcgaaggcgg ccagcttccg gggcacaagg tcgatgcgac catcgccaag acccgccact ccacgccggg tgttggcctc atttcgccgc cgccgcacca tgacatctac tcgattgaag atctggcgca gctcatctac gacctgaaaa acgtcaatcc ggcagccgat atttcggtca agctggtgtc ggaagtgggc gtgggcacgg ttgcagccgg tgtcgccaag gcgcgcgccg accatatcac cgtgtcgggc tatgatggcg gcacgggcgc ttcaccgctc acctcgctga agcacgccgg ttccccgtgg gaaatcggcc ttgccgaaac ccaccagacg ctggtgctga acgggcttcg ctcccgcgtg gcgcttcagg tcgatggcgg cttgcgcacc gggcgcgacg tggtcatcgg cgcgctgctc ggggctgacg agttcggttt ctcgaccgct ccgctgattg cggctggctg catcatgatg cgcaagtgcc atctgaacac ctgccccgtc ggcgtggcga cgcaggatcc cgtcttacgc aagcgtttca agggcacgcc ggaacacgtc atcaacttct tcttctatct ggcggaagaa gtgcgcgcgc ttttggccca gatgggtttc acccggctgg aacagatcat cggtgaaacc gagcttctgg aaaagcaggc gatgatcgac cactggaagg cgaaagggct ggatttcagc cgcatcttct acaagccgga ggccgagaag cacgaaatct actggaccga gcgccagcac cacccgatcg acgatattct ggatcgcaag ctgatcgaat tggccgcgcc tgcgctcgaa gaacgccagc cggtcaagat cgacgtggag atcaagaatg tggatcgttc cgcaggcgca atgctctcgg gcgaagtggc caagcgtttc cgccacaagg gcctgccgga agacaccatc tccgtcacgc ttcgcggtac ggctggccag tccttcggtg ccttcctcgc acgcggcatc tcgtttgaac tgatcggcga cggcaatgat tatatcggca agggcctttc aggtggccgc atcgtggtgc gcccgccgga agacacgcgc atcgtcgccg aggattcgat catcgtcggc aatacggtgc tctacggcgc gcttgaaggc gagtgctatt tccgcggcgt tgcgggcgag cgtttcgccg tgcgcaactc cggtgcggtt acggttgtcg aaggtgtggg cgaccacggc tgcgaatata tgaccggcgg cgtggttgtc gtgatcgggc agacggggcg caatttcgcg gcgggcatgt cgggcggcgt cgcctatgtg cttgatgagg aaggcgattt cgcgcagcgt tgcaacatgg cgatggtcga gctggagccg gtgccggaag aagacgacat tctggaaaag ctgcatcacc atggcggcga tctcatgcac aagggccgtg tggacgtttc cgccaacatg acccatcatg atgaggagcg tctggtgcag ctaatcgcca accacctgca ctatacgggt tcgacgcgcg caaaagacat tctcgacaat tgggaaagct atcgcccgaa attcgtgaag gtcatgccgg tggaatatcg ccgcgctctg gaagagatgg agcgcatgca gatcggcgtg gcggctgagt aa

Details for BrsuA.18150.a.A2.GE33817

HARVESTED ON:	10/4/2011
SEQUENCED ON:	10/7/2011
EXPECTED MW:	91kDa
OBSERVED MW:	91kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	97
PERCENT COVERAGE:	33

Validated AA Sequence

Validated NT Sequence

antttgtttn nctttaagaa ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgacg catttgacgc catctgtatc gaatgtggtt tctcacaacg tacagaacga agcggtcaaa gctgcgacga cggcttccac ccgaacgacg cgaaaaacca aggctgcggg tgttccgccg gctcaaggcc tttatgatcc gcgcaacgag catgatgcgt gcggcgtcgg cttcatcgcc catatgaagg gcaggaaatc acatgtggtc gtggaaaacg gcctgaagat gctggaaaac ctcacccatc gtggtgctgt cggcgccgat ccgctgatgg gcgacggtgc gggcattctg gtgcagattc cagatcgctt ctttcgcgag gaaatggcgc gtcagggcat agacctgccg cagccgggtc actatggcgt cggctatatt ttcatgccgc gcgacccgga tctgcgcgcc catgcggaag agatcgtcag ggaagtgatc gccgccgaag gccagacctt catcggcttc cgcgaagtgc cggtcgataa ttcttcgctt tccaaggctc cgcatatcgc ggccaccgag ccgttccata tgcaggtctt catcgggcgc agcccgatgc tggaaaccga cgacgatttc gagcgccgcc tgttcgtgct gcgcaaggtc atctccaacc gcatctatca ngagaatgac ggcgacgata agggtttcta tgtcgtgtcc atgtcggccc gcacggtggt ctataanggc atgttcctgg cctatcnnnc ggcgcttatt atctggactt gaaagacccc cgctttgaaa gcgcggtggc gctggtgcac cagcgttttt ccaccaacac ctttccgtcc tggngtcttg ccnaccccta ccgcatggnc gcccataacg gcgaaatcan acgctgcgcn gcantgtcaa ctngnnggng nnncgncnnn tnnnncgata ncnantnnnn nnannatntn nnnctcnnnn nntnnnnatn nnnnnnntng nnnnnnntnn nnannnnnnn nnnnnnngna tcngnnnnnn gannnnnnnt nnnnnnnnnn nnnnnnnnnn nnnnanntnn nnnnnnnnnn nnnnnnnaa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMTHLTPSVS NVVSHNVQNE AVKAATTAST RTTRKTKAAG VPPAQGLYDP RNEHDACGVG FIAHMKGRKS HVVVENGLKM LENLTHRGAV GADPLMGDGA GILVQIPDRF FREEMARQGI DLPQPGHYGV GYIFMPRDPD LRAHAEEIVR EVIAAEGQTF IGFREVPVDN SSLSKAPHIA ATEPFHMQVF IGRSPMLETD DDFERRLFVL RKVISNRIYQ ENDGDDKGFY VVSMSARTVV YKGMFLAYQV GAYYLDLKDP RFESAVALVH QRFSTNTFPS WRLAHPYRMV AHNGEINTLR GNVNWMAARQ ASVDSELFGN DISKLWPISY EGQSDTACFD NALEFLHQGG YSLAHAMMML IPEAWSGNKL MSDERRAFYE YHAALMEPWD GPAAVAFTDG RQIGAMLDRN GLRPARYIVT EDDFVILASE AGVLPVDEKK IVRKWRLQPG RMLLIDMEEG RIISDEEIKS QIAQKHPYKQ WLANTQLILE DLNPVEPRAL HKDVSLLDRQ QAFGYTQEDT KFLMAPMATT GQEAIGSMGT DTPISAMSDK SKLLYTYFKQ NFAQVTNPPI DPIREELVMS LVSFIGPRPN IFDLVGTSRR KRLEVRQPIL TNGDLEKIRS IGHTEDRFDT KTLDITYNTG EGATGMHGAI ERLCDRAEAA VHGGYNIVIL SDRQVGPDRI PIPALLATAA VHHHLIRKGL RTSVGLVVES GEPREVHHFA CLAGYGAEAI NPYLAFDTLL DMHRRREFPP EVDEHEVVKR YIKSIGKGIL KVMSKMGIST YQSYCGAQIF DAVGVR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgcatttg acgccatctg tatcgaatgt ggtttctcac aacgtacaga acgaagcggt caaagctgcg acgacggctt ccacccgaac gacgcgaaaa accaaggctg cgggtgttcc gccggctcaa ggcctttatg atccgcgcaa cgagcatgat gcgtgcggcg tcggcttcat cgcccatatg aagggcagga aatcacatgt ggtcgtggaa aacggcctga agatgctgga aaacctcacc catcgtggtg ctgtcggcgc cgatccgctg atgggcgacg gtgcgggcat tctggtgcag attccagatc gcttctttcg cgaggaaatg gcgcgtcagg gcatagacct gccgcagccg ggtcactatg gcgtcggcta tattttcatg ccgcgcgacc cggatctgcg cgcccatgcg gaagagatcg tcagggaagt gatcgccgcc gaaggccaga ccttcatcgg cttccgcgaa gtgccggtcg ataattcttc gctttccaag gctccgcata tcgcggccac cgagccgttc catatgcagg tcttcatcgg gcgcagcccg atgctggaaa ccgacgacga tttcgagcgc cgcctgttcg tgctgcgcaa ggtcatctcc aaccgcatct atcaggagaa tgacggcgac gataagggtt tctatgtcgt gtccatgtcg gcccgcacgg tggtctataa gggcatgttc ctggcctatc aggtcggcgc ttattatctg gacttgaaag acccccgctt tgaaagcgcg gtggcgctgg tgcaccagcg tttttccacc aacacctttc cgtcctggcg tcttgcccac ccctaccgca tggtcgccca taacggcgaa atcaacacgc tgcgcggcaa tgtcaactgg atggcggcgc gccaggcttc ggtcgatagc gaactgttcg gcaacgatat ttccaagctc tggccaattt cctatgaggg ccagtcggat acggcctgtt tcgacaatgc gctcgaattc ctgcatcagg gcggctacag ccttgcacat gccatgatga tgctgatccc ggaagcatgg tcgggcaaca agctgatgtc ggatgagcgc cgcgcgttct acgaatatca tgcagcactc atggagccgt gggatggccc tgccgctgtc gccttcaccg atggccgcca gatcggcgcg atgctggacc gcaacggcct gcggcctgca cgctatatcg tgacggaaga cgatttcgtc attctggctt cggaagcagg cgtcctgccg gtcgatgaaa aaaagatcgt caggaagtgg cgtcttcagc cgggccgcat gctgctgatc gacatggaag aaggccgcat catctccgat gaggaaatca agtcgcagat cgcgcagaag cacccttaca agcagtggct tgccaatacg cagctcattc tggaagacct caacccggtc gagccgcgtg cgctgcacaa ggatgtgagc ctgctcgacc gccagcaggc cttcggctat acgcaggaag ataccaagtt cctgatggcg ccgatggcga ccacgggtca ggaagccatc ggctccatgg gcaccgatac gccaatttcg gccatgtccg acaagtcgaa gctgctttac acctatttca agcagaactt cgcacaggtg acgaacccgc cgatcgatcc gatccgcgag gaactggtga tgagcctcgt ctccttcatt ggcccgcgtc caaacatctt cgatcttgtc ggcacctcgc gccgcaagcg tctggaagtg cgccagccca tcctgaccaa tggcgatctg gaaaagatcc gttctatcgg ccacacggaa gaccgcttcg ataccaagac gctcgacatc acctataata cgggcgaggg cgcgaccggt atgcatggcg ccatcgagcg tctgtgcgac cgcgcggaag cagccgttca tggcggctat aacatcgtca tcctctccga ccgccaggtc gggccggacc gtattccgat cccggccctg ctggcgacgg ctgccgtgca ccatcacctg atccgcaagg gtcttcgcac ctcggtcggt cttgtggtgg aatcgggcga gccgcgcgaa gtgcatcatt ttgcctgcct cgcaggctat ggcgccgaag cgatcaatcc ctatctcgcc ttcgacacgc tgctcgacat gcatcgccgc cgcgaatttc cgccggaagt cgacgagcat gaagtggtca agcgctacat caagtcgatt ggcaagggca tcctcaaggt catgtccaag atgggtatct cgacctatca gtcctattgc ggcgcgcaga ttttcgatgc ggtcggcgtc aggtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa

Details for BrsuA.18150.a.A3.GE33814

HARVESTED ON:	data unavailable
SEQUENCED ON:	10/7/2011
EXPECTED MW:	88kDa
OBSERVED MW:	46kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	86
PERCENT COVERAGE:	45

Validated AA Sequence

Validated NT Sequence

attttgtnnn ntttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacgg atttcgtgga caagttcttc ttcggcaccg ccacgaccat cgaaggcgtt ggcctcgacg agatcgcgga agaaaccgtg cgccgtcaca ccgatgcttt cggcaatgat cccgttcttc tgaacgcgct cgaagtcggc ggcgaatatg cctatcgtat gcgcggcgag gcgcatatgt ggtcgccgga tgcggtggca aagctgcaac atgcggtgcg cacgtccaat ccagacacgt tcaccgaata tacgaggatg ctcgacgcca agtcgtcgca ggccaagacc attcgcggcc tgttcaatat ccgctttgcc gaggaacgtg gcctgaagcc cgtttccatc gatgaggtgg agccggcaac ggatatcgtc aagcggttct caaccggcgc catgtccttc ggctcgatct ccagagaggc gcataccacg cttgcccgcg cgatgaacgc cattggcggc aagtcgaaca ccggcgaggg cggcgaggag ccggatcgtt tctacccgct gccggatggc acggcgaacc cggaacgctc ggcaatcaag caggtggcct cgggccgttt cggtgtgacg acggaatatc tcgtcaattc cgatctgatc cagatcaagg ttgcgcaggg cgcaaagccc ggcgaaggcg gccagcttcc ggggcacaag gtcgatgcga ccatcgccna gacccgccac tccacgccgg gtgttggcct catttcgccg ccgccgcacc atgacatcta ctcgattgaa gatctggcgc agctcatcta cgacctgaaa aacgtcaatc cggcagccga tatttcggtc aagctggtgt cggaagtggg cgtgggcacg gttgcagccn ggtgtcgccg cgcgccgacc atatcaccgn gtcgggctat gatngcggca cgggcgcttc nnnctcncnn nntnannacg nnnnnncccn nnngnaanca ancnancnna nnctggngct nnnnggnnnn nnnncggnnn nnnnnnnnnn nnnnnnnnnn nannnnnnnn nnnnnnnna

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMTDFVDKFF FGTATTIEGV GLDEIAEETV RRHTDAFGND PVLLNALEVG GEYAYRMRGE AHMWSPDAVA KLQHAVRTSN PDTFTEYTRM LDAKSSQAKT IRGLFNIRFA EERGLKPVSI DEVEPATDIV KRFSTGAMSF GSISREAHTT LARAMNAIGG KSNTGEGGEE PDRFYPLPDG TANPERSAIK QVASGRFGVT TEYLVNSDLI QIKVAQGAKP GEGGQLPGHK VDATIAKTRH STPGVGLISP PPHHDIYSIE DLAQLIYDLK NVNPAADISV KLVSEVGVGT VAAGVAKARA DHITVSGYDG GTGASPLTSL KHAGSPWEIG LAETHQTLVL NGLRSRVALQ VDGGLRTGRD VVIGALLGAD EFGFSTAPLI AAGCIMMRKC HLNTCPVGVA TQDPVLRKRF KGTPEHVINF FFYLAEEVRA LLAQMGFTRL EQIIGETELL EKQAMIDHWK AKGLDFSRIF YKPEAEKHEI YWTERQHHPI DDILDRKLIE LAAPALEERQ PVKIDVEIKN VDRSAGAMLS GEVAKRFRHK GLPEDTISVT LRGTAGQSFG AFLARGISFE LIGDGNDYIG KGLSGGRIVV RPPEDTRIVA EDSIIVGNTV LYGALEGECY FRGVAGERFA VRNSGAVTVV EGVGDHGCEY MTGGVVVVIG QTGRNFAAGM SGGVAYVLDE EGDFAQRCNM AMVELEPVPE EDDILEKLHH HGGDLMHKGR VDVSANMTHH DEERLVQLIA NHLHYTGSTR AKDILDNWES YRPKFVKVMP VEYRRALEEM ERMQIGVAAE

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacggatttc gtggacaagt tcttcttcgg caccgccacg accatcgaag gcgttggcct cgacgagatc gcggaagaaa ccgtgcgccg tcacaccgat gctttcggca atgatcccgt tcttctgaac gcgctcgaag tcggcggcga atatgcctat cgtatgcgcg gcgaggcgca tatgtggtcg ccggatgcgg tggcaaagct gcaacatgcg gtgcgcacgt ccaatccaga cacgttcacc gaatatacga ggatgctcga cgccaagtcg tcgcaggcca agaccattcg cggcctgttc aatatccgct ttgccgagga acgtggcctg aagcccgttt ccatcgatga ggtggagccg gcaacggata tcgtcaagcg gttctcaacc ggcgccatgt ccttcggctc gatctccaga gaggcgcata ccacgcttgc ccgcgcgatg aacgccattg gcggcaagtc gaacaccggc gagggcggcg aggagccgga tcgtttctac ccgctgccgg atggcacggc gaacccggaa cgctcggcaa tcaagcaggt ggcctcgggc cgtttcggtg tgacgacgga atatctcgtc aattccgatc tgatccagat caaggttgcg cagggcgcaa agcccggcga aggcggccag cttccggggc acaaggtcga tgcgaccatc gccaagaccc gccactccac gccgggtgtt ggcctcattt cgccgccgcc gcaccatgac atctactcga ttgaagatct ggcgcagctc atctacgacc tgaaaaacgt caatccggca gccgatattt cggtcaagct ggtgtcggaa gtgggcgtgg gcacggttgc agccggtgtc gccaaggcgc gcgccgacca tatcaccgtg tcgggctatg atggcggcac gggcgcttca ccgctcacct cgctgaagca cgccggttcc ccgtgggaaa tcggccttgc cgaaacccac cagacgctgg tgctgaacgg gcttcgctcc cgcgtggcgc ttcaggtcga tggcggcttg cgcaccgggc gcgacgtggt catcggcgcg ctgctcgggg ctgacgagtt cggtttctcg accgctccgc tgattgcggc tggctgcatc atgatgcgca agtgccatct gaacacctgc cccgtcggcg tggcgacgca ggatcccgtc ttacgcaagc gtttcaaggg cacgccggaa cacgtcatca acttcttctt ctatctggcg gaagaagtgc gcgcgctttt ggcccagatg ggtttcaccc ggctggaaca gatcatcggt gaaaccgagc ttctggaaaa gcaggcgatg atcgaccact ggaaggcgaa agggctggat ttcagccgca tcttctacaa gccggaggcc gagaagcacg aaatctactg gaccgagcgc cagcaccacc cgatcgacga tattctggat cgcaagctga tcgaattggc cgcgcctgcg ctcgaagaac gccagccggt caagatcgac gtggagatca agaatgtgga tcgttccgca ggcgcaatgc tctcgggcga agtggccaag cgtttccgcc acaagggcct gccggaagac accatctccg tcacgcttcg cggtacggct ggccagtcct tcggtgcctt cctcgcacgc ggcatctcgt ttgaactgat cggcgacggc aatgattata tcggcaaggg cctttcaggt ggccgcatcg tggtgcgccc gccggaagac acgcgcatcg tcgccgagga ttcgatcatc gtcggcaata cggtgctcta cggcgcgctt gaaggcgagt gctatttccg cggcgttgcg ggcgagcgtt tcgccgtgcg caactccggt gcggttacgg ttgtcgaagg tgtgggcgac cacggctgcg aatatatgac cggcggcgtg gttgtcgtga tcgggcagac ggggcgcaat ttcgcggcgg gcatgtcggg cggcgtcgcc tatgtgcttg atgaggaagg cgatttcgcg cagcgttgca acatggcgat ggtcgagctg gagccggtgc cggaagaaga cgacattctg gaaaagctgc atcaccatgg cggcgatctc atgcacaagg gccgtgtgga cgtttccgcc aacatgaccc atcatgatga ggagcgtctg gtgcagctaa tcgccaacca cctgcactat acgggttcga cgcgcgcaaa agacattctc gacaattggg aaagctatcg cccgaaattc gtgaaggtca tgccggtgga atatcgccgc gctctggaag agatggagcg catgcagatc ggcgtggcgg ctgagtaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa