BrsuA.18144.b

Cobalamin synthesis protein/P47K family protein

CENTER ID: BrsuA.18144.b
ORGANISM: Brucella suis 1330
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuA.18144.b.B1.GE36821 Full length( BrsuA.18144.b ) 1 390
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuA.18144.b.B1.PS01854 Full length( BrsuA.18144.b ) 1 390

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruSui107850_2070
BV-BRC: fig|204722.5.peg.2070
RefSeq: NP_699010.1
RefSeq: YP_005616834.1
UniProt: A0A0H3G4Y3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPDPIPVSVL TGFLGSGKTT LLNRLLKDPA LSDTAVIINE FGEVSIDHLL VEQASEGVIE LADGCLCCTV RGELVDTLAD LIDRLQTGRI KALKRVIIET TGLADPAPVL HSIMGHPLLT QVFRLDGVLA TVDAVNGMAT LDNHEEAVKQ AAMADRIILT KTDLPEAQAG LPALKARLRT LNPGADILEA GEERTGYAAL FECGLYNPQT KSTDVRRWLK AEAYEDEHHG HGHVCGPDCG HDHHHHDHHH DHHNDQGYAH HHHHDDAIRS FSLRHDAPIP VSTFEMFLDL LRSTHGEKLL RMKGIVQIAE DPDRPVVIHG VQKIFHPPAR LPQWPQGKRE TLLVLIVKDL PEAYVRELFD AFLGRPGLDR PDRAAMTENP LAIPGFSPRR
NT Sequence
ATGCCTGATC CCATTCCCGT TTCCGTGCTG ACCGGCTTTC TTGGCTCCGG CAAGACGACG CTGCTCAACC GGCTTTTGAA AGATCCGGCG CTCAGCGATA CAGCAGTCAT TATCAACGAG TTCGGCGAGG TGAGCATTGA CCATCTGCTG GTCGAGCAGG CGAGCGAGGG CGTGATCGAG CTGGCTGACG GGTGTCTGTG CTGCACAGTG CGCGGTGAAC TGGTCGATAC GCTGGCCGAT CTCATCGACC GGTTGCAGAC CGGCCGCATC AAGGCGCTGA AGCGCGTCAT CATCGAAACC ACGGGGCTTG CCGATCCGGC CCCGGTGCTG CATTCGATCA TGGGTCATCC TCTTCTCACG CAGGTTTTCC GGCTTGATGG CGTGCTGGCT ACCGTGGATG CGGTGAACGG CATGGCAACG CTCGACAATC ATGAGGAAGC CGTCAAACAG GCAGCCATGG CCGACCGCAT CATCCTCACC AAGACCGATC TGCCGGAAGC ACAGGCTGGC TTGCCGGCAT TGAAGGCACG GTTGCGCACG CTTAACCCCG GAGCCGATAT TCTGGAAGCG GGGGAAGAGC GCACAGGCTA TGCCGCGCTT TTTGAATGCG GGCTTTATAA CCCGCAGACG AAATCGACGG ATGTGCGGCG CTGGCTTAAG GCCGAAGCCT ATGAGGACGA GCATCATGGC CATGGCCATG TGTGCGGGCC GGATTGCGGT CACGACCATC ATCACCACGA TCACCATCAC GATCATCATA ACGATCAGGG CTATGCGCAT CATCATCATC ATGACGATGC GATCCGTTCG TTTTCACTGC GCCATGATGC GCCGATCCCG GTTTCGACCT TCGAGATGTT TCTCGATCTT CTGCGTTCGA CCCATGGTGA AAAGCTCTTG CGCATGAAGG GTATCGTGCA GATTGCCGAA GACCCGGACC GCCCGGTCGT CATCCATGGT GTGCAGAAGA TTTTCCATCC GCCCGCGCGC CTGCCGCAAT GGCCGCAAGG CAAGCGCGAA ACGCTTCTGG TCCTGATTGT GAAGGACTTG CCGGAGGCCT ATGTGCGGGA ACTTTTCGAT GCCTTTCTGG GCCGGCCCGG CCTCGACAGG CCCGACCGCG CAGCCATGAC TGAAAATCCG CTGGCCATTC CGGGGTTCTC ACCCCGCCGG
Details for BrsuA.18144.b.B1.GE36821
HARVESTED ON: 3/27/2013
SEQUENCED ON: 3/30/2013
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 66
Validated AA Sequence
MGHPLLTQVF RLDGVLATVD AVNGMATLDN HEEAVKQAAM ADRIILTKTD LPEAQAGLPA LKARLRTLNP GADILEAGEE RTGYAALFEC GLYNPQTKST DVRRWLKAEA YEDEHHGHGH VCGPDCGHDH HHHDHHHDHH NDQGYAHHHH HDDAIRSFSL RHDAPIPVST FEMFLDLLRS THGEKLLRMK GIVQIAEDPD RPVVIHGVQK IFHPPARLPQ WPQGKRETLL VLIVKDLPEA YVRELFDAFL GRPGLDRPDR AAMTE
Validated NT Sequence
attttcagtc atggctgcgc ggtcgggcct gtcgaggccg ggccggccca gaaaggcatc gaaaagttcc cgcacatagg cctccggcaa gtccttcaca atcaggacca gaagcgtttc gcgcttgcct tgcggccatt gcggcaggcg cgcgggcgga tggaaaatct tctgcacacc atggatgacg accgggcggt ccgggtcttc ggcaatctgc acgataccct tcatgcgcaa gagcttttca ccatgggtcg aacgcagaag atcgagaaac atctcgaagg tcgaaaccgg gatcggcgca tcatggcgca gtgaaaacga acggatcgca tcgtcatgat gatgatgatg cgcatagccc tgatcgttat gatgatcgtg atggtgatcg tggtgatgat ggtcgtgacc gcaatccggc ccgcacacat ggccatggcc atgatgctcg tcctcatagg cttcggcctt aagccagcgc cgcacatccg tcgatttcgt ctgcgggtta taaagcccgc attcaaaaag cgcggcatag cctgtgcgct cttcccccgc ttccagaata tcggctccgg ggttaagcgt gcgcaaccgt gccttcaatg ccggcaagcc agcctgtgct tccggcagat cggtcttggt gaggatgatg cggtcggcca tggctgcctg tttgacggct tcctcatgat tgtcgagcgt tgccatgccg ttcaccgcat ccacggtagc cagcacgcca tcaagccgga aaacctgcgt gagaagagga tgacccatga tcgaatgcag caccggggcc ggatcggcaa gccccgtggt ttcgatgatg acgcgcttca gcgccttgat gcggccggtc tgcaaccggt cgatgagatc ggccagcgta tcgaccagtt caccgcgcac tgtgcagcac agacacccgt cagccagctc gatcacgccc tcgctcgcct gctcgaccag cagatggtca atgctcacct cgccgaactc gttgataatg actgctgtat cgctgagcgc cggatctttc aaaagccggt tgagcagcgt cgtcttgccg gagccaagaa agccggtcag cacggaaacg ggaatggg
Expected Protein Sequence
MAHHHHHHMP DPIPVSVLTG FLGSGKTTLL NRLLKDPALS DTAVIINEFG EVSIDHLLVE QASEGVIELA DGCLCCTVRG ELVDTLADLI DRLQTGRIKA LKRVIIETTG LADPAPVLHS IMGHPLLTQV FRLDGVLATV DAVNGMATLD NHEEAVKQAA MADRIILTKT DLPEAQAGLP ALKARLRTLN PGADILEAGE ERTGYAALFE CGLYNPQTKS TDVRRWLKAE AYEDEHHGHG HVCGPDCGHD HHHHDHHHDH HNDQGYAHHH HHDDAIRSFS LRHDAPIPVS TFEMFLDLLR STHGEKLLRM KGIVQIAEDP DRPVVIHGVQ KIFHPPARLP QWPQGKRETL LVLIVKDLPE AYVRELFDAF LGRPGLDRPD RAAMTENPLA IPGFSPRR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcctg atcccattcc cgtttccgtg ctgaccggct ttcttggctc cggcaagacg acgctgctca accggctttt gaaagatccg gcgctcagcg atacagcagt cattatcaac gagttcggcg aggtgagcat tgaccatctg ctggtcgagc aggcgagcga gggcgtgatc gagctggctg acgggtgtct gtgctgcaca gtgcgcggtg aactggtcga tacgctggcc gatctcatcg accggttgca gaccggccgc atcaaggcgc tgaagcgcgt catcatcgaa accacggggc ttgccgatcc ggccccggtg ctgcattcga tcatgggtca tcctcttctc acgcaggttt tccggcttga tggcgtgctg gctaccgtgg atgcggtgaa cggcatggca acgctcgaca atcatgagga agccgtcaaa caggcagcca tggccgaccg catcatcctc accaagaccg atctgccgga agcacaggct ggcttgccgg cattgaaggc acggttgcgc acgcttaacc ccggagccga tattctggaa gcgggggaag agcgcacagg ctatgccgcg ctttttgaat gcgggcttta taacccgcag acgaaatcga cggatgtgcg gcgctggctt aaggccgaag cctatgagga cgagcatcat ggccatggcc atgtgtgcgg gccggattgc ggtcacgacc atcatcacca cgatcaccat cacgatcatc ataacgatca gggctatgcg catcatcatc atcatgacga tgcgatccgt tcgttttcac tgcgccatga tgcgccgatc ccggtttcga ccttcgagat gtttctcgat cttctgcgtt cgacccatgg tgaaaagctc ttgcgcatga agggtatcgt gcagattgcc gaagacccgg accgcccggt cgtcatccat ggtgtgcaga agattttcca tccgcccgcg cgcctgccgc aatggccgca aggcaagcgc gaaacgcttc tggtcctgat tgtgaaggac ttgccggagg cctatgtgcg ggaacttttc gatgcctttc tgggccggcc cggcctcgac aggcccgacc gcgcagccat gactgaaaat ccgctggcca ttccggggtt ctcaccccgc cggtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for BrsuA.18144.b.B1.PS01854
PURIFICATION DATe: 6/27/2013
CONCENTRATION: 15mg/ml
OBSERVED MW: 45kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 66
Protocol Notes
notes unavailable
Validated AA Sequence
MGHPLLTQVF RLDGVLATVD AVNGMATLDN HEEAVKQAAM ADRIILTKTD LPEAQAGLPA LKARLRTLNP GADILEAGEE RTGYAALFEC GLYNPQTKST DVRRWLKAEA YEDEHHGHGH VCGPDCGHDH HHHDHHHDHH NDQGYAHHHH HDDAIRSFSL RHDAPIPVST FEMFLDLLRS THGEKLLRMK GIVQIAEDPD RPVVIHGVQK IFHPPARLPQ WPQGKRETLL VLIVKDLPEA YVRELFDAFL GRPGLDRPDR AAMTE
Validated NT Sequence
attttcagtc atggctgcgc ggtcgggcct gtcgaggccg ggccggccca gaaaggcatc gaaaagttcc cgcacatagg cctccggcaa gtccttcaca atcaggacca gaagcgtttc gcgcttgcct tgcggccatt gcggcaggcg cgcgggcgga tggaaaatct tctgcacacc atggatgacg accgggcggt ccgggtcttc ggcaatctgc acgataccct tcatgcgcaa gagcttttca ccatgggtcg aacgcagaag atcgagaaac atctcgaagg tcgaaaccgg gatcggcgca tcatggcgca gtgaaaacga acggatcgca tcgtcatgat gatgatgatg cgcatagccc tgatcgttat gatgatcgtg atggtgatcg tggtgatgat ggtcgtgacc gcaatccggc ccgcacacat ggccatggcc atgatgctcg tcctcatagg cttcggcctt aagccagcgc cgcacatccg tcgatttcgt ctgcgggtta taaagcccgc attcaaaaag cgcggcatag cctgtgcgct cttcccccgc ttccagaata tcggctccgg ggttaagcgt gcgcaaccgt gccttcaatg ccggcaagcc agcctgtgct tccggcagat cggtcttggt gaggatgatg cggtcggcca tggctgcctg tttgacggct tcctcatgat tgtcgagcgt tgccatgccg ttcaccgcat ccacggtagc cagcacgcca tcaagccgga aaacctgcgt gagaagagga tgacccatga tcgaatgcag caccggggcc ggatcggcaa gccccgtggt ttcgatgatg acgcgcttca gcgccttgat gcggccggtc tgcaaccggt cgatgagatc ggccagcgta tcgaccagtt caccgcgcac tgtgcagcac agacacccgt cagccagctc gatcacgccc tcgctcgcct gctcgaccag cagatggtca atgctcacct cgccgaactc gttgataatg actgctgtat cgctgagcgc cggatctttc aaaagccggt tgagcagcgt cgtcttgccg gagccaagaa agccggtcag cacggaaacg ggaatggg
Expressed Protein Sequence
MAHHHHHHMP DPIPVSVLTG FLGSGKTTLL NRLLKDPALS DTAVIINEFG EVSIDHLLVE QASEGVIELA DGCLCCTVRG ELVDTLADLI DRLQTGRIKA LKRVIIETTG LADPAPVLHS IMGHPLLTQV FRLDGVLATV DAVNGMATLD NHEEAVKQAA MADRIILTKT DLPEAQAGLP ALKARLRTLN PGADILEAGE ERTGYAALFE CGLYNPQTKS TDVRRWLKAE AYEDEHHGHG HVCGPDCGHD HHHHDHHHDH HNDQGYAHHH HHDDAIRSFS LRHDAPIPVS TFEMFLDLLR STHGEKLLRM KGIVQIAEDP DRPVVIHGVQ KIFHPPARLP QWPQGKRETL LVLIVKDLPE AYVRELFDAF LGRPGLDRPD RAAMTENPLA IPGFSPRR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCTG ATCCCATTCC CGTTTCCGTG CTGACCGGCT TTCTTGGCTC CGGCAAGACG ACGCTGCTCA ACCGGCTTTT GAAAGATCCG GCGCTCAGCG ATACAGCAGT CATTATCAAC GAGTTCGGCG AGGTGAGCAT TGACCATCTG CTGGTCGAGC AGGCGAGCGA GGGCGTGATC GAGCTGGCTG ACGGGTGTCT GTGCTGCACA GTGCGCGGTG AACTGGTCGA TACGCTGGCC GATCTCATCG ACCGGTTGCA GACCGGCCGC ATCAAGGCGC TGAAGCGCGT CATCATCGAA ACCACGGGGC TTGCCGATCC GGCCCCGGTG CTGCATTCGA TCATGGGTCA TCCTCTTCTC ACGCAGGTTT TCCGGCTTGA TGGCGTGCTG GCTACCGTGG ATGCGGTGAA CGGCATGGCA ACGCTCGACA ATCATGAGGA AGCCGTCAAA CAGGCAGCCA TGGCCGACCG CATCATCCTC ACCAAGACCG ATCTGCCGGA AGCACAGGCT GGCTTGCCGG CATTGAAGGC ACGGTTGCGC ACGCTTAACC CCGGAGCCGA TATTCTGGAA GCGGGGGAAG AGCGCACAGG CTATGCCGCG CTTTTTGAAT GCGGGCTTTA TAACCCGCAG ACGAAATCGA CGGATGTGCG GCGCTGGCTT AAGGCCGAAG CCTATGAGGA CGAGCATCAT GGCCATGGCC ATGTGTGCGG GCCGGATTGC GGTCACGACC ATCATCACCA CGATCACCAT CACGATCATC ATAACGATCA GGGCTATGCG CATCATCATC ATCATGACGA TGCGATCCGT TCGTTTTCAC TGCGCCATGA TGCGCCGATC CCGGTTTCGA CCTTCGAGAT GTTTCTCGAT CTTCTGCGTT CGACCCATGG TGAAAAGCTC TTGCGCATGA AGGGTATCGT GCAGATTGCC GAAGACCCGG ACCGCCCGGT CGTCATCCAT GGTGTGCAGA AGATTTTCCA TCCGCCCGCG CGCCTGCCGC AATGGCCGCA AGGCAAGCGC GAAACGCTTC TGGTCCTGAT TGTGAAGGAC TTGCCGGAGG CCTATGTGCG GGAACTTTTC GATGCCTTTC TGGGCCGGCC CGGCCTCGAC AGGCCCGACC GCGCAGCCAT GACTGAAAAT CCGCTGGCCA TTCCGGGGTT CTCACCCCGC CGGTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT