BrsuA.18143.a

Phosphoglucomutase (EC 5.4.2.2)

CENTER ID: BrsuA.18143.a
ORGANISM: Brucella suis 1330
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuA.18143.a.A1.GE33820 full length 1 543
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuA.18143.a.A1.PW34430 full length 1 543

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|204722.5.peg.62
RefSeq: NP_697100.1
UniProt: A0A0H3G9F1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTVKTIATTP YQDQQPGTSG LRKKVPVFQQ PNYAENFIQS IFDVLEGFKG KTLVVGGDGR FYNREVIQKL IKIAAANGFG RIMVGQGGIL STPAASNMIR KYKAFGGMIL SASHNPGGPT QDFGIKYNIG NGGPAPEKIT EAIFARSKVI DQYKIADAAD IDIDKIGTSK IGDTEVVIFD PVADYAELME SLFDFAAIRA MIKGGFQMKF DAMHAVTGPY AKEIFERRLG APEGSVVNFV PLPDFGGHHP DPNLVYAKDL YDLLMSSHAP DFGAASDGDG DRNLILGRGI FITPSDSLAM LAANAHLAPG YKGGIKGIAR SMPTSAAADR VAEKLGIGMY ETPTGWKFFG NLLDSGKVTI CGEESSGTGS DHVREKDGLW AVLLWLNILA VRKESVKAIA DDHWARFGRN YYTRHDYEAV DSDIATKLVA DLRGKLAGLP GTSVNGLRIE KADDFAYHDP VDGSTSEHQG IRIYFEGGAR IVLRLSGTGT SGATIRIYIE RYEADPAKHN LDTQATLAPL IDAAEQIAEV KKRSGRTEPS VVT
NT Sequence
atgaccgtca agaccattgc gaccacgcca taccaggatc aacagccggg cacttcgggc ttgcgcaaga aggttccggt tttccagcag cccaattatg cagaaaattt catccagtcg atcttcgacg tgctggaggg gttcaagggc aagacgctcg tggttggcgg cgacggacgc ttttataacc gcgaggtgat ccagaagctt atcaagatcg cagccgccaa cggttttggc cgtatcatgg tgggacaggg cggcattctt tccacgccag ccgcctccaa catgatccgc aagtacaaag ccttcggtgg catgatcctt tcggccagcc acaatccggg cgggcctacg caggatttcg gcatcaagta caatatcggc aatggtggac cggcacccga aaagatcacc gaagcgatct tcgcccgctc aaaggtgatc gaccagtata aaattgcaga tgcagccgat atcgatatcg acaagatcgg cacaagcaag atcggcgata ccgaggtggt catcttcgat ccggtggccg attatgctga attgatggaa agcctgttcg atttcgccgc catccgcgcc atgataaaag gcggcttcca gatgaagttc gacgccatgc atgcggtgac cgggccttat gccaaggaaa tcttcgagcg ccgcctcggt gcgcccgaag gcagcgtcgt caatttcgtg cccctgccgg atttcggtgg ccatcacccg gatccaaacc tcgtctatgc caaggatctc tacgatcttc tgatgtccag ccatgcgccg gattttggcg ccgcctccga cggcgatggc gatcgcaacc tcattctcgg gcgcggtatc ttcatcacgc cgtcagattc gcttgccatg ctggccgcaa atgcccatct ggcgccgggc tataaaggcg gtatcaaggg cattgcccgt tccatgccga cgagcgccgc cgccgaccgc gtggcggaaa aactcggtat cggcatgtat gaaaccccga ccggctggaa attcttcggc aatcttctcg acagcggcaa ggtgacgatc tgcggcgagg aaagctccgg caccggctcc gaccatgtgc gcgaaaaaga tggtttgtgg gccgttctgc tctggctcaa cattctggcg gtgcgcaagg aaagtgtgaa ggcaatcgcg gacgaccatt gggcacgttt cgggcgcaat tattacactc gccacgacta tgaagccgtc gattccgata ttgcgacgaa gctggtggct gacctgcgcg gcaagcttgc cggcctgccc ggcacgagcg tcaatggcct caggatcgaa aaggccgacg acttcgccta tcatgatccc gttgatggct cgaccagcga acatcagggc atccgcatct attttgaagg aggtgcgcgc atcgtgcttc gcctgtccgg taccgggacg tcaggcgcga ccatccgcat ctatatcgaa cgctatgaag ccgatcctgc aaaacataat ctcgacacgc aggcaacgct ggccccactg atcgacgcgg cggaacagat cgccgaagta aaaaaacgca gcgggcggac agagccgtcg gtggttactt ga
Details for BrsuA.18143.a.A1.GE33820
HARVESTED ON: 10/4/2011
SEQUENCED ON: 10/7/2011
EXPECTED MW: 60kDa
OBSERVED MW: 60kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 96
Validated AA Sequence
MTVKTIATTP YQDQQPGTSG LRKKVPVFQQ PNYAENFIQS IFDVLEGFKG KTLVVGGDGR FYNREVIQKL IKIAAANGFG RIMVGQGGIL STPAASNMIR KYKAFGGMIL SASHNPGGPT QDFGIKYNIG NGGPAPEKIT EAIFARSKVI DQYKIADAAD IDIDKIGTSK IGDTEVVIFD PVADYAELME SLFDFAAIRA MIKGGFQMKF DAMHAVTGPY AKEIFERRLG APEGSVVNFV PLPXFGGHHP DPNLVYAXDL YDLLMSSHAP XFGAASDGDG DRNLILGRGI FITPSDSLAM LAANAHLAPG YKGGIKGIAR SMPTSAAADR VAEKLGIGMY ETPTGWKFFG NLLDSGKVTI CGEESSGTGS DHVREKDGLW AVLLWLNILA VRKESVKAIA DDHWARFGRN YYTRHDYEAV DSDIATKLVA DLRGKLAGLP GTSVNGLRIE KADDFAYHDP VDGSTSEHQG IRIYFEGGAR IVLRLSGTGT SGATIRIYIE RYEADPAKHN LDTQATLAPL IDAAEQIAEV KKRSGRTEPS VVT
Validated NT Sequence
ttaagtaacc accgacggct ctgtccgccc gctgcgtttt tttacttcgg cgatctgttc cgccgcgtcg atcagtgggg ccagcgttgc ctgcgtgtcg agattatgtt ttgcaggatc ggcttcatag cgttcgatat agatgcggat ggtcgcgcct gacgtcccgg taccggacag gcgaagcacg atgcgcgcac ctccttcaaa atagatgcgg atgccctgat gttcgctggt cgagccatca acgggatcat gataggcgaa gtcgtcggcc ttttcgatcc tgaggccatt gacgctcgtg ccgggcaggc cggcaagctt gccgcgcagg tcagccacca gcttcgtcgc aatatcggaa tcgacggctt catagtcgtg gcgagtgtaa taattgcgcc cgaaacgtgc ccaatggtcg tccgcgattg ccttcacact ttccttgcgc accgccagaa tgttgagcca gagcagaacg gcccacaaac catctttttc gcgcacatgg tcggagccgg tgccggagct ttcctcgccg cagatcgtca ccttgccgct gtcgagaaga ttgccgaaga atttccagcc ggtcggggtt tcatacatgc cgataccgag tttttccgcc acgcggtcgg cggcggcgct cgtcggcatg gaacgggcaa tgcccttgat accgccttta tagcccggcg ccagatgggc atttgcggcc agcatggcaa gcgaatctga cggcgtgatg aagataccgc gcccgagaat gaggttgcga tcgccatcgc cgtcggaggc ggcgccaaan tccggcgcat ggctggacat cagaagatcg tagagatccn tggcatagac gaggttngga tccgggtgat ggccaccgaa ntccggcagg ggcacgaaat tgacgacgct gccttcgggc gcaccgaggc ggcgctcgaa gatttccttg gcataaggcc cggtcaccgc atgcatggcg tcgaacttca tctggaagcc gccttttatc atggcgcgga tggcggcgaa atcgaacagg ctttccatca attcagcata atcggccacc ggatcgaaga tgaccacctc ggtatcgccg atcttgcttg tgccgatctt gtcgatatcg atatcggctg catctgcaat tttatactgg tcgatcacct ttgagcgggc gaagatcgct tcggtgatct tttcgggtgc cggtccacca ttgccgatat tgtacttgat gccgaaatcc tgcgtaggcc cgcccggatt gtggctggcc gaaaggatca tgccaccgaa ggctttgtac ttgcggatca tgttggaggc ggctggcgtg gaaagaatgc cgccctgtcc caccatgata cggccaaaac cgttggcggc tgcgatcttg ataagcttct ggatcacctc gcggttataa aagcgtccgt cgccgccaac cacgagcgtc ttgcccttga acccctccag cacgtcgaag atcgactgga tgaaattttc tgcataattg ggctgctgga aaaccggaac cttcttgcgc aagcccgaag tgcccggctg ttgatcctgg tatggcgtgg tcgcaatggt cttgacggtc atcgaaccag gaccctgggt ctgagcttcc agggtaccca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTVKTIATT PYQDQQPGTS GLRKKVPVFQ QPNYAENFIQ SIFDVLEGFK GKTLVVGGDG RFYNREVIQK LIKIAAANGF GRIMVGQGGI LSTPAASNMI RKYKAFGGMI LSASHNPGGP TQDFGIKYNI GNGGPAPEKI TEAIFARSKV IDQYKIADAA DIDIDKIGTS KIGDTEVVIF DPVADYAELM ESLFDFAAIR AMIKGGFQMK FDAMHAVTGP YAKEIFERRL GAPEGSVVNF VPLPDFGGHH PDPNLVYAKD LYDLLMSSHA PDFGAASDGD GDRNLILGRG IFITPSDSLA MLAANAHLAP GYKGGIKGIA RSMPTSAAAD RVAEKLGIGM YETPTGWKFF GNLLDSGKVT ICGEESSGTG SDHVREKDGL WAVLLWLNIL AVRKESVKAI ADDHWARFGR NYYTRHDYEA VDSDIATKLV ADLRGKLAGL PGTSVNGLRI EKADDFAYHD PVDGSTSEHQ GIRIYFEGGA RIVLRLSGTG TSGATIRIYI ERYEADPAKH NLDTQATLAP LIDAAEQIAE VKKRSGRTEP SVVT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgtcaag accattgcga ccacgccata ccaggatcaa cagccgggca cttcgggctt gcgcaagaag gttccggttt tccagcagcc caattatgca gaaaatttca tccagtcgat cttcgacgtg ctggaggggt tcaagggcaa gacgctcgtg gttggcggcg acggacgctt ttataaccgc gaggtgatcc agaagcttat caagatcgca gccgccaacg gttttggccg tatcatggtg ggacagggcg gcattctttc cacgccagcc gcctccaaca tgatccgcaa gtacaaagcc ttcggtggca tgatcctttc ggccagccac aatccgggcg ggcctacgca ggatttcggc atcaagtaca atatcggcaa tggtggaccg gcacccgaaa agatcaccga agcgatcttc gcccgctcaa aggtgatcga ccagtataaa attgcagatg cagccgatat cgatatcgac aagatcggca caagcaagat cggcgatacc gaggtggtca tcttcgatcc ggtggccgat tatgctgaat tgatggaaag cctgttcgat ttcgccgcca tccgcgccat gataaaaggc ggcttccaga tgaagttcga cgccatgcat gcggtgaccg ggccttatgc caaggaaatc ttcgagcgcc gcctcggtgc gcccgaaggc agcgtcgtca atttcgtgcc cctgccggat ttcggtggcc atcacccgga tccaaacctc gtctatgcca aggatctcta cgatcttctg atgtccagcc atgcgccgga ttttggcgcc gcctccgacg gcgatggcga tcgcaacctc attctcgggc gcggtatctt catcacgccg tcagattcgc ttgccatgct ggccgcaaat gcccatctgg cgccgggcta taaaggcggt atcaagggca ttgcccgttc catgccgacg agcgccgccg ccgaccgcgt ggcggaaaaa ctcggtatcg gcatgtatga aaccccgacc ggctggaaat tcttcggcaa tcttctcgac agcggcaagg tgacgatctg cggcgaggaa agctccggca ccggctccga ccatgtgcgc gaaaaagatg gtttgtgggc cgttctgctc tggctcaaca ttctggcggt gcgcaaggaa agtgtgaagg caatcgcgga cgaccattgg gcacgtttcg ggcgcaatta ttacactcgc cacgactatg aagccgtcga ttccgatatt gcgacgaagc tggtggctga cctgcgcggc aagcttgccg gcctgcccgg cacgagcgtc aatggcctca ggatcgaaaa ggccgacgac ttcgcctatc atgatcccgt tgatggctcg accagcgaac atcagggcat ccgcatctat tttgaaggag gtgcgcgcat cgtgcttcgc ctgtccggta ccgggacgtc aggcgcgacc atccgcatct atatcgaacg ctatgaagcc gatcctgcaa aacataatct cgacacgcag gcaacgctgg ccccactgat cgacgcggcg gaacagatcg ccgaagtaaa aaaacgcagc gggcggacag agccgtcggt ggttacttaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for BrsuA.18143.a.A1.PW34430
PURIFICATION DATe: 12/2/2011
CONCENTRATION: 48.48mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 96
Protocol Notes
notes unavailable
Validated AA Sequence
MTVKTIATTP YQDQQPGTSG LRKKVPVFQQ PNYAENFIQS IFDVLEGFKG KTLVVGGDGR FYNREVIQKL IKIAAANGFG RIMVGQGGIL STPAASNMIR KYKAFGGMIL SASHNPGGPT QDFGIKYNIG NGGPAPEKIT EAIFARSKVI DQYKIADAAD IDIDKIGTSK IGDTEVVIFD PVADYAELME SLFDFAAIRA MIKGGFQMKF DAMHAVTGPY AKEIFERRLG APEGSVVNFV PLPXFGGHHP DPNLVYAXDL YDLLMSSHAP XFGAASDGDG DRNLILGRGI FITPSDSLAM LAANAHLAPG YKGGIKGIAR SMPTSAAADR VAEKLGIGMY ETPTGWKFFG NLLDSGKVTI CGEESSGTGS DHVREKDGLW AVLLWLNILA VRKESVKAIA DDHWARFGRN YYTRHDYEAV DSDIATKLVA DLRGKLAGLP GTSVNGLRIE KADDFAYHDP VDGSTSEHQG IRIYFEGGAR IVLRLSGTGT SGATIRIYIE RYEADPAKHN LDTQATLAPL IDAAEQIAEV KKRSGRTEPS VVT
Validated NT Sequence
ttaagtaacc accgacggct ctgtccgccc gctgcgtttt tttacttcgg cgatctgttc cgccgcgtcg atcagtgggg ccagcgttgc ctgcgtgtcg agattatgtt ttgcaggatc ggcttcatag cgttcgatat agatgcggat ggtcgcgcct gacgtcccgg taccggacag gcgaagcacg atgcgcgcac ctccttcaaa atagatgcgg atgccctgat gttcgctggt cgagccatca acgggatcat gataggcgaa gtcgtcggcc ttttcgatcc tgaggccatt gacgctcgtg ccgggcaggc cggcaagctt gccgcgcagg tcagccacca gcttcgtcgc aatatcggaa tcgacggctt catagtcgtg gcgagtgtaa taattgcgcc cgaaacgtgc ccaatggtcg tccgcgattg ccttcacact ttccttgcgc accgccagaa tgttgagcca gagcagaacg gcccacaaac catctttttc gcgcacatgg tcggagccgg tgccggagct ttcctcgccg cagatcgtca ccttgccgct gtcgagaaga ttgccgaaga atttccagcc ggtcggggtt tcatacatgc cgataccgag tttttccgcc acgcggtcgg cggcggcgct cgtcggcatg gaacgggcaa tgcccttgat accgccttta tagcccggcg ccagatgggc atttgcggcc agcatggcaa gcgaatctga cggcgtgatg aagataccgc gcccgagaat gaggttgcga tcgccatcgc cgtcggaggc ggcgccaaan tccggcgcat ggctggacat cagaagatcg tagagatccn tggcatagac gaggttngga tccgggtgat ggccaccgaa ntccggcagg ggcacgaaat tgacgacgct gccttcgggc gcaccgaggc ggcgctcgaa gatttccttg gcataaggcc cggtcaccgc atgcatggcg tcgaacttca tctggaagcc gccttttatc atggcgcgga tggcggcgaa atcgaacagg ctttccatca attcagcata atcggccacc ggatcgaaga tgaccacctc ggtatcgccg atcttgcttg tgccgatctt gtcgatatcg atatcggctg catctgcaat tttatactgg tcgatcacct ttgagcgggc gaagatcgct tcggtgatct tttcgggtgc cggtccacca ttgccgatat tgtacttgat gccgaaatcc tgcgtaggcc cgcccggatt gtggctggcc gaaaggatca tgccaccgaa ggctttgtac ttgcggatca tgttggaggc ggctggcgtg gaaagaatgc cgccctgtcc caccatgata cggccaaaac cgttggcggc tgcgatcttg ataagcttct ggatcacctc gcggttataa aagcgtccgt cgccgccaac cacgagcgtc ttgcccttga acccctccag cacgtcgaag atcgactgga tgaaattttc tgcataattg ggctgctgga aaaccggaac cttcttgcgc aagcccgaag tgcccggctg ttgatcctgg tatggcgtgg tcgcaatggt cttgacggtc atcgaaccag gaccctgggt ctgagcttcc agggtaccca
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTVKTIATT PYQDQQPGTS GLRKKVPVFQ QPNYAENFIQ SIFDVLEGFK GKTLVVGGDG RFYNREVIQK LIKIAAANGF GRIMVGQGGI LSTPAASNMI RKYKAFGGMI LSASHNPGGP TQDFGIKYNI GNGGPAPEKI TEAIFARSKV IDQYKIADAA DIDIDKIGTS KIGDTEVVIF DPVADYAELM ESLFDFAAIR AMIKGGFQMK FDAMHAVTGP YAKEIFERRL GAPEGSVVNF VPLPDFGGHH PDPNLVYAKD LYDLLMSSHA PDFGAASDGD GDRNLILGRG IFITPSDSLA MLAANAHLAP GYKGGIKGIA RSMPTSAAAD RVAEKLGIGM YETPTGWKFF GNLLDSGKVT ICGEESSGTG SDHVREKDGL WAVLLWLNIL AVRKESVKAI ADDHWARFGR NYYTRHDYEA VDSDIATKLV ADLRGKLAGL PGTSVNGLRI EKADDFAYHD PVDGSTSEHQ GIRIYFEGGA RIVLRLSGTG TSGATIRIYI ERYEADPAKH NLDTQATLAP LIDAAEQIAE VKKRSGRTEP SVVT
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCGTCAAG ACCATTGCGA CCACGCCATA CCAGGATCAA CAGCCGGGCA CTTCGGGCTT GCGCAAGAAG GTTCCGGTTT TCCAGCAGCC CAATTATGCA GAAAATTTCA TCCAGTCGAT CTTCGACGTG CTGGAGGGGT TCAAGGGCAA GACGCTCGTG GTTGGCGGCG ACGGACGCTT TTATAACCGC GAGGTGATCC AGAAGCTTAT CAAGATCGCA GCCGCCAACG GTTTTGGCCG TATCATGGTG GGACAGGGCG GCATTCTTTC CACGCCAGCC GCCTCCAACA TGATCCGCAA GTACAAAGCC TTCGGTGGCA TGATCCTTTC GGCCAGCCAC AATCCGGGCG GGCCTACGCA GGATTTCGGC ATCAAGTACA ATATCGGCAA TGGTGGACCG GCACCCGAAA AGATCACCGA AGCGATCTTC GCCCGCTCAA AGGTGATCGA CCAGTATAAA ATTGCAGATG CAGCCGATAT CGATATCGAC AAGATCGGCA CAAGCAAGAT CGGCGATACC GAGGTGGTCA TCTTCGATCC GGTGGCCGAT TATGCTGAAT TGATGGAAAG CCTGTTCGAT TTCGCCGCCA TCCGCGCCAT GATAAAAGGC GGCTTCCAGA TGAAGTTCGA CGCCATGCAT GCGGTGACCG GGCCTTATGC CAAGGAAATC TTCGAGCGCC GCCTCGGTGC GCCCGAAGGC AGCGTCGTCA ATTTCGTGCC CCTGCCGGAT TTCGGTGGCC ATCACCCGGA TCCAAACCTC GTCTATGCCA AGGATCTCTA CGATCTTCTG ATGTCCAGCC ATGCGCCGGA TTTTGGCGCC GCCTCCGACG GCGATGGCGA TCGCAACCTC ATTCTCGGGC GCGGTATCTT CATCACGCCG TCAGATTCGC TTGCCATGCT GGCCGCAAAT GCCCATCTGG CGCCGGGCTA TAAAGGCGGT ATCAAGGGCA TTGCCCGTTC CATGCCGACG AGCGCCGCCG CCGACCGCGT GGCGGAAAAA CTCGGTATCG GCATGTATGA AACCCCGACC GGCTGGAAAT TCTTCGGCAA TCTTCTCGAC AGCGGCAAGG TGACGATCTG CGGCGAGGAA AGCTCCGGCA CCGGCTCCGA CCATGTGCGC GAAAAAGATG GTTTGTGGGC CGTTCTGCTC TGGCTCAACA TTCTGGCGGT GCGCAAGGAA AGTGTGAAGG CAATCGCGGA CGACCATTGG GCACGTTTCG GGCGCAATTA TTACACTCGC CACGACTATG AAGCCGTCGA TTCCGATATT GCGACGAAGC TGGTGGCTGA CCTGCGCGGC AAGCTTGCCG GCCTGCCCGG CACGAGCGTC AATGGCCTCA GGATCGAAAA GGCCGACGAC TTCGCCTATC ATGATCCCGT TGATGGCTCG ACCAGCGAAC ATCAGGGCAT CCGCATCTAT TTTGAAGGAG GTGCGCGCAT CGTGCTTCGC CTGTCCGGTA CCGGGACGTC AGGCGCGACC ATCCGCATCT ATATCGAACG CTATGAAGCC GATCCTGCAA AACATAATCT CGACACGCAG GCAACGCTGG CCCCACTGAT CGACGCGGCG GAACAGATCG CCGAAGTAAA AAAACGCAGC GGGCGGACAG AGCCGTCGGT GGTTACTTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA