BrsuA.18142.a
Hydrolase, haloacid dehalogenase-like family
| CENTER ID: | BrsuA.18142.a |
| ORGANISM: | Brucella suis 1330 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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You may order multiple materials at a time at no cost to you, as this contract is funded
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrsuA.18142.a.A1.GE33813 | full length | 1 | 283 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
6NQ4
DEPOSITED: 1/19/2019
DETERMINATION: XRay
CLONE: BrsuA.18142.a.A1.GE33813
PROTEIN: BrsuA.18142.a.A1.PS01295
Molecular models
| METHOD | RESULTS |
|---|---|
| comparative modelling | Robetta_80581 |
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|204722.5.peg.2414 |
| RefSeq: | NP_699400.1 |
| UniProt: | A0A0H3GA96 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMILPERLDD LTDRYDAIFC DVWGVVHNGE TSFAPAIAAL QRARAKGVTI ILVTNSPRPH PGVVAQMSLL GVPENAYDRV VTSGDVTRDL IAEGPRRIFH IGCERELAIY DGLDVELVEE FEAAGVVCTG LYDDEVETPE DYRELLQRLR SRNLPFICAN PDIMVERGPR LIWCAGALAR EYGQLGGRTL IAGKPHRPIY EAALRAVESI RGGSVDKSRI LGIGDGVLTD VKGAADFGLD VLYISGGVHA ADYAVNGDLD MAKMEAFLEK HGHRPIASLH ALV
NT Sequence
atgatgatac tgcccgagcg cctcgacgat ctgacggacc gatatgatgc gatcttttgc gacgtctggg gcgttgtaca caacggcgaa acgtcctttg cgccggccat cgccgccttg cagcgcgctc gcgccaaggg tgtgacaatc attctcgtga ccaattctcc acgcccgcat ccgggcgttg tcgcgcagat gtcgcttctg ggtgtgcccg aaaatgccta tgaccgtgtt gtcacttccg gtgatgttac gcgcgatctt atcgccgaag ggccgcgccg gattttccac atcggctgcg agcgcgagct tgccatttat gatgggcttg acgtggagct ggtcgaggaa tttgaggcgg caggcgtggt ttgtaccggg ctttatgacg atgaagtcga aacgccagaa gactacaggg aattgctcca gcgtctgcgc tcgcgcaacc tgcctttcat ttgcgccaat cctgatatca tggtggaacg cgggcctcgc ctcatctggt gcgcgggcgc gcttgcacgc gaatacggcc agcttggagg gcgcaccctg attgcaggca agccccatcg cccgatctat gaggcggcgc ttcgcgcggt ggaaagcatt cgcggtggaa gcgtggacaa gagccgcatt cttggcattg gcgacggcgt tttgaccgat gtgaaagggg ctgcggattt tggcctcgat gtgctttata tttcgggcgg cgttcatgcc gccgattatg cggtgaacgg ggatctcgat atggcgaaga tggaggcttt tctggaaaag catggccatc gccccattgc ttctctacat gcgcttgtct ga
Details for BrsuA.18142.a.A1.GE33813
| HARVESTED ON: | 10/4/2011 |
| SEQUENCED ON: | 10/7/2011 |
| EXPECTED MW: | 33kDa |
| OBSERVED MW: | 33kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMMILPERLD DLTDRYDAIF CDVWGVVHNG ETSFAPAIAA LQRARAKGVT IILVTNSPRP HPGVVAQMSL LGVPENAYDR VVTSGDVTRD LIAEGPRRIF HIGCERELAI YDGLDVELVE EFEAAGVVCT GLYDDEVETP EDYRELLQRL RSRNLPFICA NPDIMVERGP RLIWCAGALA REYGQLGGRT LIAGKPHRPI YEAALRAVES IRGGSVDKSR ILGIGDGVLT DVKGAADFGL DVLYISGGVH AADYAVNGDL DMAKMEAFLE KHGHRPIASL HALV
Validated NT Sequence
ttattagaca agcgcatgta gagaagcaat ggggcgatgg ccatgctttt ccagaaaagc ctccatcttc gccatatcga gatccccgtt caccgcataa tcggcggcat gaacgccgcc cgaaatataa agcacatcga ggccaaaatc cgcagcccct ttcacatcgg tcaaaacgcc gtcgccaatg ccaagaatgc ggctcttgtc cacgcttcca ccgcgaatgc tttccaccgc gcgaagcgcc gcctcataga tcgggcgatg gggcttgcct gcaatcaggg tgcgccctcc aagctggccg tattcgcgtg caagcgcgcc cgcgcaccag atgaggcgag gcccgcgttc caccatgata tcaggattgg cgcaaatgaa aggcaggttg cgcgagcgca gacgctggag caattccctg tagtcttctg gcgtttcgac ttcatcgtca taaagcccgg tacaaaccac gcctgccgcc tcaaattcct cgaccagctc cacgtcaagc ccatcataaa tggcaagctc gcgctcgcag ccgatgtgga aaatccggcg cggcccttcg gcgataagat cgcgcgtaac atcaccggaa gtgacaacac ggtcataggc attttcgggc acacccagaa gcgacatctg cgcgacaacg cccggatgcg ggcgtggaga attggtcacg agaatgattg tcacaccctt ggcgcgagcg cgctgcaagg cggcgatggc cggcgcaaag gacgtttcgc cgttgtgtac aacgccccag acgtcgcaaa agatcgcatc atatcggtcc gtcagatcgt cgaggcgctc gggcagtatc atcatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMMILPERLD DLTDRYDAIF CDVWGVVHNG ETSFAPAIAA LQRARAKGVT IILVTNSPRP HPGVVAQMSL LGVPENAYDR VVTSGDVTRD LIAEGPRRIF HIGCERELAI YDGLDVELVE EFEAAGVVCT GLYDDEVETP EDYRELLQRL RSRNLPFICA NPDIMVERGP RLIWCAGALA REYGQLGGRT LIAGKPHRPI YEAALRAVES IRGGSVDKSR ILGIGDGVLT DVKGAADFGL DVLYISGGVH AADYAVNGDL DMAKMEAFLE KHGHRPIASL HALV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatgatactg cccgagcgcc tcgacgatct gacggaccga tatgatgcga tcttttgcga cgtctggggc gttgtacaca acggcgaaac gtcctttgcg ccggccatcg ccgccttgca gcgcgctcgc gccaagggtg tgacaatcat tctcgtgacc aattctccac gcccgcatcc gggcgttgtc gcgcagatgt cgcttctggg tgtgcccgaa aatgcctatg accgtgttgt cacttccggt gatgttacgc gcgatcttat cgccgaaggg ccgcgccgga ttttccacat cggctgcgag cgcgagcttg ccatttatga tgggcttgac gtggagctgg tcgaggaatt tgaggcggca ggcgtggttt gtaccgggct ttatgacgat gaagtcgaaa cgccagaaga ctacagggaa ttgctccagc gtctgcgctc gcgcaacctg cctttcattt gcgccaatcc tgatatcatg gtggaacgcg ggcctcgcct catctggtgc gcgggcgcgc ttgcacgcga atacggccag cttggagggc gcaccctgat tgcaggcaag ccccatcgcc cgatctatga ggcggcgctt cgcgcggtgg aaagcattcg cggtggaagc gtggacaaga gccgcattct tggcattggc gacggcgttt tgaccgatgt gaaaggggct gcggattttg gcctcgatgt gctttatatt tcgggcggcg ttcatgccgc cgattatgcg gtgaacgggg atctcgatat ggcgaagatg gaggcttttc tggaaaagca tggccatcgc cccattgctt ctctacatgc gcttgtctaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa