SSGCID: Target Page

BrsuA.18138.a

EAL domain protein

CENTER ID:	BrsuA.18138.a
ORGANISM:	Brucella suis 1330
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuA.18138.a.A2.GE33816	domain1		29	249

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|204722.5.peg.501
RefSeq:	NP_697515.1
UniProt:	A0A0H3G276

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRFRRNSLIS PVFLVLLLML ILGAVAGQLV GKGLRILEEE RHMDIYMTAL RDYSSRLLAS ARNTLEAANN SPFEICSPQD LAYQRKLVFA AYHIKDIGRL RDDQLICSTL LSDIKTQPRR SVANVNLKDG TYIYADDALI TPGSHGPIIG RGNANVVLSS VAFDLLHTPR YAFSIFATDE EKKHFALLYN YSGNAEPGPE PSSGDWAQKF VEAPDGKVLR ENSCDPDTGV CISLSATIDR TSTAARLKNL LAICLGALAG GSVGLGWLYY RNRDHSLLYR LNKALAAGEP HLLYQPVVDM ADGKVLGFEA LIRWEIRKGD FVPPDVFVAR AEEAGTATKI TIYVLERLIE DMGDTLRQRR DLRININITA SDLQSPAFMS RMERRLAAAD IEPQQVGLEL TERTAVDFSK ATDGIRRLRE QGHRIYIDDF GTGYSSLAYL GELHVDAIKI DKAFTRTVGN DYATVSIIPQ IISMAKEHGL DIVVEGVETE AQALYFRKLG TPLAAQGWFF GKPLCVSDAQ ALVATVKKKQ SRVKLARKTT QKAK

NT Sequence

atgcggttcc gtcgcaatag tttgatttcc cccgttttcc ttgttctgct tctcatgctt attctggggg ccgtggccgg gcaactggtc ggaaaaggac tccgcattct cgaagaagaa cgtcatatgg atatctatat gacggctctg cgcgattact ccagccggct gctcgcctcc gcgaggaata ctctggaggc tgcgaataat tctcccttcg agatatgttc cccgcaggat cttgcctatc agcgcaagct cgtctttgcc gcctatcaca tcaaggatat cgggcgtctg cgcgatgacc aacttatctg ctcgacgctt ctgagtgata tcaaaactca gccgcgccgt tccgttgcca atgtcaattt gaaggacgga acctatatct atgccgatga tgccttgata acgccgggta gccatgggcc gatcatcgga cgagggaatg cgaatgtggt cttgagctcg gttgccttcg atctcctgca cacgccgcgc tatgcttttt ccatttttgc caccgacgag gaaaagaagc atttcgcgct gctttacaat tattcaggta atgccgagcc tggacctgag ccgtcctctg gtgattgggc gcagaaattc gtggaagcgc cggatggcaa ggtgttgcgg gagaatagct gtgacccgga tacgggcgtc tgcatcagcc tgtcagcaac gatcgaccga accagcacgg cagcacggct gaaaaacctg ctggccatct gccttggcgc attggcgggt ggcagtgtcg ggctgggctg gctctattat cgcaatcgcg accactcatt gctctacagg ctgaacaagg cactcgctgc cggagagccg catttgctct accagccggt ggtggatatg gcggatggca aggtgctcgg ctttgaagca ttgatccgat gggaaatccg caagggcgat ttcgtcccgc cggatgtctt cgttgcgcgg gcggaggagg cgggcaccgc tacaaagata acgatctatg tgctggaacg tctgatagaa gacatgggcg acacgctgcg ccagcgccgt gatttgcgta tcaatatcaa cattaccgcc agcgacctgc aaagcccggc cttcatgagc cggatggaaa ggcgccttgc ggcggctgat atcgaaccac aacaggtggg gttggaactg acggaacgca cggcggtcga tttttccaag gcgacggacg gcatcaggcg cctgcgcgag caggggcacc ggatttatat cgacgatttc ggcaccggct attcgagcct tgcctatctc ggcgaattgc atgtggacgc aatcaagatc gacaaggctt tcacgcgcac tgtcggcaat gattacgcaa cggtctccat cattccgcag atcatttcca tggcgaagga acatggcctc gatatcgtgg tcgaaggggt ggaaacggag gcacaggccc tctattttcg taaactgggc acgcccctgg ccgcacaagg ctggtttttt ggcaagccgc tgtgcgtttc cgatgcgcag gcgctggtcg ctacagtgaa gaagaagcaa tcgcgcgtga agttggcccg caaaacgacc caaaaggcga aatga

Details for BrsuA.18138.a.A2.GE33816

HARVESTED ON:	10/4/2011
SEQUENCED ON:	10/7/2011
EXPECTED MW:	26kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLVGKGLRI LEEERHMDIY MTALRDYSSR LLASARNTLE AANNSPFEIC SPQDLAYQRK LVFAAYHIKD IGRLRDDQLI CSTLLSDIKT QPRRSVANVN LKDGTYIYAD DALITPGSHG PIIGRGNANV VLSSVAFDLL HTPRYAFSIF ATDEEKKHFA LLYNYSGNAE PGPEPSSGDW AQKFVEAPDG KVLRENSCDP DTGVCISLSA TIDRTSTAAR LKN

Validated NT Sequence

ttntttnnct ttaagaagga ganataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgctggtc ggaaaaggac tccgcattct cgaagaagaa cgtcatatgg atatctatat gacggctctg cgcgattact ccagccggct gctcgcctcc gcgaggaata ctctggaggc tgcgaataat tctcccttcg agatatgttc cccgcaggat cttgcctatc agcgcaagct cgtctttgcc gcctatcaca tcaaggatat cgggcgtctg cgcgatgacc aacttatctg ctcgacgctt ctgagtgata tcaaaactca gccgcgccgt tccgttgcca atgtcaattt gaaggacgga acctatatct atgccgatga tgccttgata acgccgggta gccatgggcc gatcatcgga cgagggaatg cgaatgtggt cttgagctcg gttgccttcg atctcctgca cacgccgcgc tatgcttttt ccatttttgc caccgacgag gaaaagaagc atttcgcgct gctttacaat tattcaggta atgccgagcc tggacctgag ccgtcctctg gtgattgggc gcagaaattc gtggaagcgc cggatggcaa ggtgttgcgg gagaatagct gtgacccgga tacgggcgtc tgcatcagcc tgtcagcaac gatcgaccga accagcacgg cagcacggct gaaaaactaa taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctganttggc tgctgcnnnn nnnnnnnnnn nnnnncnngt gnnnnnnnnc ntgctgcnnn nnnnncnnng nnnncnntnn nnncnntacn nnngnannnn nnngntnnnn nnnnctgnnn nnngnnnnnn nnnnncnnnn nnannnnnnc nnnnnnnnnn nnntnnnnnn nnnnnnnnnn ncnnnnnggn nntnnnncan nnnnnnnnnn nnnncnnnnn nnnnncnnnn tntnnnnnnc naannnnnnn nnnnnnnnnn ntnnnannnn ncc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctggtcgga aaaggactcc gcattctcga agaagaacgt catatggata tctatatgac ggctctgcgc gattactcca gccggctgct cgcctccgcg aggaatactc tggaggctgc gaataattct cccttcgaga tatgttcccc gcaggatctt gcctatcagc gcaagctcgt ctttgccgcc tatcacatca aggatatcgg gcgtctgcgc gatgaccaac ttatctgctc gacgcttctg agtgatatca aaactcagcc gcgccgttcc gttgccaatg tcaatttgaa ggacggaacc tatatctatg ccgatgatgc cttgataacg ccgggtagcc atgggccgat catcggacga gggaatgcga atgtggtctt gagctcggtt gccttcgatc tcctgcacac gccgcgctat gctttttcca tttttgccac cgacgaggaa aagaagcatt tcgcgctgct ttacaattat tcaggtaatg ccgagcctgg acctgagccg tcctctggtg attgggcgca gaaattcgtg gaagcgccgg atggcaaggt gttgcgggag aatagctgtg acccggatac gggcgtctgc atcagcctgt cagcaacgat cgaccgaacc agcacggcag cacggctgaa aaactaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa