BrsuA.18136.a

TPR domain protein

CENTER ID: BrsuA.18136.a
ORGANISM: Brucella suis 1330
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuA.18136.a.A1.GE33825 full length 1 621
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuA.18136.a.A1.PS01288 full length 1 621

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|204722.5.peg.417
RefSeq: NP_697433.1
UniProt: A0A0H3G1Z3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRLGSKHRPL YGLLLSLLAG IAISTSGALA KTTPDPVRAQ VRAGSFAGAY LAGRTAESDD NLSAAVDFYR QAMAFDPNNS QIKQDLMLVL LTEGRFKDAL PLAHELQNVT AIERFSRLAL AIDAINRQQY RKVGPLLMFS LQSDMDRLLI NLMNAWVKVG QGNPKEALAD VQKMDGPEWY DLFRTYNAAL IADLAGRKQE ARDYYQQAID DRRGGSAAPD TYERVVMAFA AFKLRQGDRA GAIKTLKDAE ELLNGRMTIT EMREKIEAGH KYGRLVKTAQ DGAAETLYTL GTAINRSGAE AFAKLYLQMS LPLRPDNDAT LYQLGDISAK LHEPQKAVEY YGRVPEQSPY RRDAEMQRAL NLAENDQSAE AIKQLKVLLG RDKSDMRTYL ALGGVYAQDK NFADAAKIYD AAVEQIKTPA RKDWPLFYQR GIAYERLQEW DKAEPNFRKA LELYPDQPQV LNYLGYSWVD RNENLDEALN MIRKAVELRP QDGYIVDSLG WAYYQLGRYN DAVVELEKAV KLRPEDPTIN DHMGDAYWRV GRLLEATFQW NHAIAGKPEP EELAKIKEKL KKGLPDIPGQ SAADATKPAG KKPDEAKPEA PAPSATPAAP SDKAPEKPTT D
NT Sequence
atgcggctag gatcgaaaca tcgcccgctt tatggattgc tgctctcgct tctggcgggc atcgcaattt cgacgagcgg agcattggcc aagaccacac ccgatcccgt tcgcgcgcag gtccgcgccg gatccttcgc gggcgcttat cttgcgggcc gcacggctga atccgacgac aacctttccg ctgccgtgga tttctatcgt caggccatgg ctttcgatcc gaacaacagc cagatcaagc aggacctgat gctggttctg ctgaccgaag gccgtttcaa ggatgcgttg ccgcttgccc atgaattgca gaatgtgacg gccatcgagc ggttttctcg gctggcactg gccattgatg cgatcaaccg ccagcaatat cgcaaggtcg gcccgcttct catgttctcg ctccagtcgg acatggaccg gctgttgatc aatttgatga atgcctgggt gaaggtcggg cagggcaatc cgaaagaggc gctggccgat gtgcagaaaa tggatgggcc ggaatggtac gatcttttcc gcacctataa tgccgcgttg attgccgatc ttgcaggccg caagcaggaa gcgcgggact attaccagca ggccatcgac gaccgccgcg gcggcagcgc cgcgcccgac acttatgagc gcgtggtcat ggcctttgcc gcgttcaagc tgcgccaggg cgacagggca ggtgcgatca agacgctgaa ggatgccgaa gagcttctga atggccgcat gaccatcact gaaatgcgcg agaagatcga ggcgggccac aaatatggcc gcttggtcaa gaccgcgcag gacggggccg ccgaaacgct ctatacgctg ggcacagcca tcaaccgcag cggtgcggaa gctttcgcca agctctatct ccagatgtcg ctgccgcttc gcccggataa cgacgccacg ctctaccagc ttggcgatat ttcggcaaag ttgcacgagc cgcaaaaggc cgtcgaatat tatggccgcg tgccggaaca atcgccctat cgccgcgatg cggaaatgca gcgcgcgctc aatctggcgg aaaacgacca gtcggcggaa gccatcaagc agttgaaagt cctgcttggc cgcgacaagt ccgacatgcg cacctatctg gcgctgggcg gcgtttatgc gcaggacaag aattttgcgg atgcggcaaa gatttacgat gcggcggtcg agcagatcaa gacgccggca cgcaaggatt ggccactctt ctatcagcgc ggcatcgcct atgaacggct acaggaatgg gacaaggcgg agcccaattt ccgcaaggcg ctggagcttt atcccgatca gccgcaggtt ctgaactatc ttggttattc ctgggtggac cggaacgaaa acctcgatga agccctgaac atgatcagga aagcggtcga gttgcgcccg caggacggct atatcgttga ttcgcttggc tgggcctatt accagcttgg ccgttataac gacgctgtgg tcgaactgga aaaggccgtg aagctgcgcc cggaagaccc gaccatcaac gaccatatgg gcgatgccta ttggcgggtc ggtcgtctgc ttgaagcaac cttccagtgg aaccatgcca ttgcgggcaa gccggaaccg gaagaactgg cgaaaatcaa ggaaaagctg aagaagggcc tgccggatat tccgggccag tcggctgccg atgcaacaaa gcctgccggg aaaaagccag atgaggcaaa gccggaagcg cctgctccat cggccacgcc ggctgcacct tccgacaagg ccccggaaaa gccgacaacc gattga
Details for BrsuA.18136.a.A1.GE33825
HARVESTED ON: 10/4/2011
SEQUENCED ON: 10/7/2011
EXPECTED MW: 71kDa
OBSERVED MW: 71kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 77
PERCENT COVERAGE: 59
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRLGSKHRP LYGLLLSLLA GIAISTSGAL AKTTPDPVRA QVRAGSFAGA YLAGRTAESD DNLSAAVDFY RQAMAFDPNN SQIKQDLMLV LLTEGRFKDA LPLAHELQNV TAIERFSRLA LAIDAINRQQ YRKVGPLLMF SLQSDMDRLL INLMNAWVKV GQGNPKEALA DVQKMDGPEW YDLFRTYNAA LIADLAGRKQ EARDYYQQAI DDRRGGSAAP DTYERVVMAF AAFKLRQGDR AGAIKTLXDA EELLNGRMTI TEMREKIEAG HKYXPLGQDR AXGAAXRSIR WAQPXXQRXX FXXSISDXXX XXXXXXXLXX XXXXXXXXXX KXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXX
Validated NT Sequence
attttntnnn ntttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcggc taggatcgaa acatcgcccg ctttatggat tgctgctctc gcttctggcg ggcatcgcaa tttcgacgag cggagcattg gccaagacca cacccgatcc cgttcgcgcg caggtccgcg ccggatcctt cgcgggcgct tatcttgcgg gccgcacggc tgaatccgac gacaaccttt ccgctgccgt ggatttctat cgtcaggcca tggctttcga tccgaacaac agccagatca agcaggacct gatgctggtt ctgctgaccg aaggccgttt caaggatgcg ttgccgcttg cccatgaatt gcagaatgtg acggccatcg agcggttttc tcggctggca ctggccattg atgcgatcaa ccgccagcaa tatcgcaagg tcggcccgct tctcatgttc tcgctccagt cggacatgga ccggctgttg atcaatttga tgaatgcctg ggtgaaggtc gggcagggca atccgaaaga ggcgctggcc gatgtgcaga aaatggatgg gccggaatgg tacgatcttt tccgcaccta taatgccgcg ttgattgccg atcttgcagg ccgcaagcag gaagcgcggg actattacca gcaggccatc gacgaccgcc gcggcggcag cgccgcgccc gacacttatg agcgcgtggt catggccttt gccgcgttca agctgcgcca gggcgacagg gcaggtgcga tcaagacgct gaangatgcc gaagagcttc tgaatggccg catgaccatc actgaaatgc gcgagaagat cgaggcgggc cacaaatatn ggccgcttgg ncaagaccgc gcnnnngggg ccgccgancg ctctatacgc tgggcacagc catnancgca gcgngnganc tttcnnnnct ctatctcnga tgncnntncn nttcnccnna nannncncnc gctntncnnc tnnnannttt nnncnaannn nnnnnnnnnc aaannnncna annnnnnnnn nnnnnnnnnn annnnnnnnn nnnnnnnncn nnnnnnnngn nnnnnnnnnn nnnnnnncnn nnnnnnnnnc nnnancnnaa nnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRLGSKHRP LYGLLLSLLA GIAISTSGAL AKTTPDPVRA QVRAGSFAGA YLAGRTAESD DNLSAAVDFY RQAMAFDPNN SQIKQDLMLV LLTEGRFKDA LPLAHELQNV TAIERFSRLA LAIDAINRQQ YRKVGPLLMF SLQSDMDRLL INLMNAWVKV GQGNPKEALA DVQKMDGPEW YDLFRTYNAA LIADLAGRKQ EARDYYQQAI DDRRGGSAAP DTYERVVMAF AAFKLRQGDR AGAIKTLKDA EELLNGRMTI TEMREKIEAG HKYGRLVKTA QDGAAETLYT LGTAINRSGA EAFAKLYLQM SLPLRPDNDA TLYQLGDISA KLHEPQKAVE YYGRVPEQSP YRRDAEMQRA LNLAENDQSA EAIKQLKVLL GRDKSDMRTY LALGGVYAQD KNFADAAKIY DAAVEQIKTP ARKDWPLFYQ RGIAYERLQE WDKAEPNFRK ALELYPDQPQ VLNYLGYSWV DRNENLDEAL NMIRKAVELR PQDGYIVDSL GWAYYQLGRY NDAVVELEKA VKLRPEDPTI NDHMGDAYWR VGRLLEATFQ WNHAIAGKPE PEELAKIKEK LKKGLPDIPG QSAADATKPA GKKPDEAKPE APAPSATPAA PSDKAPEKPT TD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcggctagga tcgaaacatc gcccgcttta tggattgctg ctctcgcttc tggcgggcat cgcaatttcg acgagcggag cattggccaa gaccacaccc gatcccgttc gcgcgcaggt ccgcgccgga tccttcgcgg gcgcttatct tgcgggccgc acggctgaat ccgacgacaa cctttccgct gccgtggatt tctatcgtca ggccatggct ttcgatccga acaacagcca gatcaagcag gacctgatgc tggttctgct gaccgaaggc cgtttcaagg atgcgttgcc gcttgcccat gaattgcaga atgtgacggc catcgagcgg ttttctcggc tggcactggc cattgatgcg atcaaccgcc agcaatatcg caaggtcggc ccgcttctca tgttctcgct ccagtcggac atggaccggc tgttgatcaa tttgatgaat gcctgggtga aggtcgggca gggcaatccg aaagaggcgc tggccgatgt gcagaaaatg gatgggccgg aatggtacga tcttttccgc acctataatg ccgcgttgat tgccgatctt gcaggccgca agcaggaagc gcgggactat taccagcagg ccatcgacga ccgccgcggc ggcagcgccg cgcccgacac ttatgagcgc gtggtcatgg cctttgccgc gttcaagctg cgccagggcg acagggcagg tgcgatcaag acgctgaagg atgccgaaga gcttctgaat ggccgcatga ccatcactga aatgcgcgag aagatcgagg cgggccacaa atatggccgc ttggtcaaga ccgcgcagga cggggccgcc gaaacgctct atacgctggg cacagccatc aaccgcagcg gtgcggaagc tttcgccaag ctctatctcc agatgtcgct gccgcttcgc ccggataacg acgccacgct ctaccagctt ggcgatattt cggcaaagtt gcacgagccg caaaaggccg tcgaatatta tggccgcgtg ccggaacaat cgccctatcg ccgcgatgcg gaaatgcagc gcgcgctcaa tctggcggaa aacgaccagt cggcggaagc catcaagcag ttgaaagtcc tgcttggccg cgacaagtcc gacatgcgca cctatctggc gctgggcggc gtttatgcgc aggacaagaa ttttgcggat gcggcaaaga tttacgatgc ggcggtcgag cagatcaaga cgccggcacg caaggattgg ccactcttct atcagcgcgg catcgcctat gaacggctac aggaatggga caaggcggag cccaatttcc gcaaggcgct ggagctttat cccgatcagc cgcaggttct gaactatctt ggttattcct gggtggaccg gaacgaaaac ctcgatgaag ccctgaacat gatcaggaaa gcggtcgagt tgcgcccgca ggacggctat atcgttgatt cgcttggctg ggcctattac cagcttggcc gttataacga cgctgtggtc gaactggaaa aggccgtgaa gctgcgcccg gaagacccga ccatcaacga ccatatgggc gatgcctatt ggcgggtcgg tcgtctgctt gaagcaacct tccagtggaa ccatgccatt gcgggcaagc cggaaccgga agaactggcg aaaatcaagg aaaagctgaa gaagggcctg ccggatattc cgggccagtc ggctgccgat gcaacaaagc ctgccgggaa aaagccagat gaggcaaagc cggaagcgcc tgctccatcg gccacgccgg ctgcaccttc cgacaaggcc ccggaaaagc cgacaaccga ttaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for BrsuA.18136.a.A1.PS01288
PURIFICATION DATe: 12/1/2011
CONCENTRATION: 56.9mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 110µl
PERCENT IDENTITY: 77
PERCENT COVERAGE: 59
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRLGSKHRP LYGLLLSLLA GIAISTSGAL AKTTPDPVRA QVRAGSFAGA YLAGRTAESD DNLSAAVDFY RQAMAFDPNN SQIKQDLMLV LLTEGRFKDA LPLAHELQNV TAIERFSRLA LAIDAINRQQ YRKVGPLLMF SLQSDMDRLL INLMNAWVKV GQGNPKEALA DVQKMDGPEW YDLFRTYNAA LIADLAGRKQ EARDYYQQAI DDRRGGSAAP DTYERVVMAF AAFKLRQGDR AGAIKTLXDA EELLNGRMTI TEMREKIEAG HKYXPLGQDR AXGAAXRSIR WAQPXXQRXX FXXSISDXXX XXXXXXXLXX XXXXXXXXXX KXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXX
Validated NT Sequence
attttntnnn ntttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcggc taggatcgaa acatcgcccg ctttatggat tgctgctctc gcttctggcg ggcatcgcaa tttcgacgag cggagcattg gccaagacca cacccgatcc cgttcgcgcg caggtccgcg ccggatcctt cgcgggcgct tatcttgcgg gccgcacggc tgaatccgac gacaaccttt ccgctgccgt ggatttctat cgtcaggcca tggctttcga tccgaacaac agccagatca agcaggacct gatgctggtt ctgctgaccg aaggccgttt caaggatgcg ttgccgcttg cccatgaatt gcagaatgtg acggccatcg agcggttttc tcggctggca ctggccattg atgcgatcaa ccgccagcaa tatcgcaagg tcggcccgct tctcatgttc tcgctccagt cggacatgga ccggctgttg atcaatttga tgaatgcctg ggtgaaggtc gggcagggca atccgaaaga ggcgctggcc gatgtgcaga aaatggatgg gccggaatgg tacgatcttt tccgcaccta taatgccgcg ttgattgccg atcttgcagg ccgcaagcag gaagcgcggg actattacca gcaggccatc gacgaccgcc gcggcggcag cgccgcgccc gacacttatg agcgcgtggt catggccttt gccgcgttca agctgcgcca gggcgacagg gcaggtgcga tcaagacgct gaangatgcc gaagagcttc tgaatggccg catgaccatc actgaaatgc gcgagaagat cgaggcgggc cacaaatatn ggccgcttgg ncaagaccgc gcnnnngggg ccgccgancg ctctatacgc tgggcacagc catnancgca gcgngnganc tttcnnnnct ctatctcnga tgncnntncn nttcnccnna nannncncnc gctntncnnc tnnnannttt nnncnaannn nnnnnnnnnc aaannnncna annnnnnnnn nnnnnnnnnn annnnnnnnn nnnnnnnncn nnnnnnnngn nnnnnnnnnn nnnnnnncnn nnnnnnnnnc nnnancnnaa nnc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRLGSKHRP LYGLLLSLLA GIAISTSGAL AKTTPDPVRA QVRAGSFAGA YLAGRTAESD DNLSAAVDFY RQAMAFDPNN SQIKQDLMLV LLTEGRFKDA LPLAHELQNV TAIERFSRLA LAIDAINRQQ YRKVGPLLMF SLQSDMDRLL INLMNAWVKV GQGNPKEALA DVQKMDGPEW YDLFRTYNAA LIADLAGRKQ EARDYYQQAI DDRRGGSAAP DTYERVVMAF AAFKLRQGDR AGAIKTLKDA EELLNGRMTI TEMREKIEAG HKYGRLVKTA QDGAAETLYT LGTAINRSGA EAFAKLYLQM SLPLRPDNDA TLYQLGDISA KLHEPQKAVE YYGRVPEQSP YRRDAEMQRA LNLAENDQSA EAIKQLKVLL GRDKSDMRTY LALGGVYAQD KNFADAAKIY DAAVEQIKTP ARKDWPLFYQ RGIAYERLQE WDKAEPNFRK ALELYPDQPQ VLNYLGYSWV DRNENLDEAL NMIRKAVELR PQDGYIVDSL GWAYYQLGRY NDAVVELEKA VKLRPEDPTI NDHMGDAYWR VGRLLEATFQ WNHAIAGKPE PEELAKIKEK LKKGLPDIPG QSAADATKPA GKKPDEAKPE APAPSATPAA PSDKAPEKPT TD
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCGGCTAGGA TCGAAACATC GCCCGCTTTA TGGATTGCTG CTCTCGCTTC TGGCGGGCAT CGCAATTTCG ACGAGCGGAG CATTGGCCAA GACCACACCC GATCCCGTTC GCGCGCAGGT CCGCGCCGGA TCCTTCGCGG GCGCTTATCT TGCGGGCCGC ACGGCTGAAT CCGACGACAA CCTTTCCGCT GCCGTGGATT TCTATCGTCA GGCCATGGCT TTCGATCCGA ACAACAGCCA GATCAAGCAG GACCTGATGC TGGTTCTGCT GACCGAAGGC CGTTTCAAGG ATGCGTTGCC GCTTGCCCAT GAATTGCAGA ATGTGACGGC CATCGAGCGG TTTTCTCGGC TGGCACTGGC CATTGATGCG ATCAACCGCC AGCAATATCG CAAGGTCGGC CCGCTTCTCA TGTTCTCGCT CCAGTCGGAC ATGGACCGGC TGTTGATCAA TTTGATGAAT GCCTGGGTGA AGGTCGGGCA GGGCAATCCG AAAGAGGCGC TGGCCGATGT GCAGAAAATG GATGGGCCGG AATGGTACGA TCTTTTCCGC ACCTATAATG CCGCGTTGAT TGCCGATCTT GCAGGCCGCA AGCAGGAAGC GCGGGACTAT TACCAGCAGG CCATCGACGA CCGCCGCGGC GGCAGCGCCG CGCCCGACAC TTATGAGCGC GTGGTCATGG CCTTTGCCGC GTTCAAGCTG CGCCAGGGCG ACAGGGCAGG TGCGATCAAG ACGCTGAAGG ATGCCGAAGA GCTTCTGAAT GGCCGCATGA CCATCACTGA AATGCGCGAG AAGATCGAGG CGGGCCACAA ATATGGCCGC TTGGTCAAGA CCGCGCAGGA CGGGGCCGCC GAAACGCTCT ATACGCTGGG CACAGCCATC AACCGCAGCG GTGCGGAAGC TTTCGCCAAG CTCTATCTCC AGATGTCGCT GCCGCTTCGC CCGGATAACG ACGCCACGCT CTACCAGCTT GGCGATATTT CGGCAAAGTT GCACGAGCCG CAAAAGGCCG TCGAATATTA TGGCCGCGTG CCGGAACAAT CGCCCTATCG CCGCGATGCG GAAATGCAGC GCGCGCTCAA TCTGGCGGAA AACGACCAGT CGGCGGAAGC CATCAAGCAG TTGAAAGTCC TGCTTGGCCG CGACAAGTCC GACATGCGCA CCTATCTGGC GCTGGGCGGC GTTTATGCGC AGGACAAGAA TTTTGCGGAT GCGGCAAAGA TTTACGATGC GGCGGTCGAG CAGATCAAGA CGCCGGCACG CAAGGATTGG CCACTCTTCT ATCAGCGCGG CATCGCCTAT GAACGGCTAC AGGAATGGGA CAAGGCGGAG CCCAATTTCC GCAAGGCGCT GGAGCTTTAT CCCGATCAGC CGCAGGTTCT GAACTATCTT GGTTATTCCT GGGTGGACCG GAACGAAAAC CTCGATGAAG CCCTGAACAT GATCAGGAAA GCGGTCGAGT TGCGCCCGCA GGACGGCTAT ATCGTTGATT CGCTTGGCTG GGCCTATTAC CAGCTTGGCC GTTATAACGA CGCTGTGGTC GAACTGGAAA AGGCCGTGAA GCTGCGCCCG GAAGACCCGA CCATCAACGA CCATATGGGC GATGCCTATT GGCGGGTCGG TCGTCTGCTT GAAGCAACCT TCCAGTGGAA CCATGCCATT GCGGGCAAGC CGGAACCGGA AGAACTGGCG AAAATCAAGG AAAAGCTGAA GAAGGGCCTG CCGGATATTC CGGGCCAGTC GGCTGCCGAT GCAACAAAGC CTGCCGGGAA AAAGCCAGAT GAGGCAAAGC CGGAAGCGCC TGCTCCATCG GCCACGCCGG CTGCACCTTC CGACAAGGCC CCGGAAAAGC CGACAACCGA TTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA