SSGCID: Target Page

BrsuA.18134.a

Erythritol phosphate dehydrogenase (EC 1.1.5.3)

CENTER ID:	BrsuA.18134.a
ORGANISM:	Brucella suis 1330
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuA.18134.a.A1.GE33826	full length		1	502

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuA.18134.a.A1.PW34436	full length		1	502

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|204722.5.peg.3082
RefSeq:	NP_700033.1
UniProt:	A0A0H3G7C1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAEPETCDLF VIGGGINGAG VARDAAGRGL KVVLAEKDDL AQGTSSRSGK LVHGGLRYLE YYEFRLVREA LIEREVLSNA APHIIWPMRF VLPHSPQDRP AWLVRLGLFL YDHLGGRKKL PGTRTLDLKR DPEGTPILDQ YTKGFEYSDC WVDDARLVAL NAVGAAEKGA TILTRTPVVS ARRENGGWIV ETRNSDTGET RTFRARCIVN CAGPWVTDVI HNVAASTSSR NVRLVKGSHI IVPKFWSGAN AYLVQNHDKR VIFINPYEGD KALIGTTDIA YEGRAEDVAA DEKEIDYLIT AVNRYFKEKL RREDVLHSFS GVRPLFDDGK GNPSAVTRDY VFDLDETGGA PLLNVFGGKI TTFRELAERG MHRLKHIFPQ MGGDWTHDAP LPGGEIANAD YETFANTLRD IYPWMPRTLV HHYGRLYGAR TKDVVAGAQN LEGLGRHFGG DFHEAEVRYL VAREWAKTAE DILYRRTKHY LHLTEAERAA FVEWFDNANL VA

NT Sequence

atggctgaac cggaaacctg cgacctgttt gtaattggcg gcggcatcaa cggtgcgggc gtggcccgcg acgcggccgg gcgcggcctc aaggtggtgc tggcagaaaa ggacgatctg gcgcaaggca catcatcgcg ctccggcaag ctggtgcatg gcggtttgcg ctatctcgaa tattatgagt tccgccttgt gcgcgaagcg ctgatcgagc gcgaagtgct ttcgaatgcg gcaccccata tcatctggcc gatgcgcttc gtgctgccgc acagcccgca ggatcgcccg gcctggctgg tgcggcttgg cctgtttctc tatgaccatc tgggcgggcg caaaaaactt cccggcacgc gtacgctcga tctgaagcgc gacccggaag gcacgccgat cctcgaccag tacaccaagg gttttgaata ttccgattgc tgggtggacg atgcgcgcct cgtggcgctc aatgcggtgg gtgccgccga aaagggcgcg accattctca cccgtacacc ggtcgtctcc gcacgccgcg aaaatggcgg ctggatcgtg gaaaccagaa acagcgatac cggcgaaacc cgcaccttcc gcgcccgctg catcgtcaat tgcgccggac catgggtcac ggacgtcatc cacaatgtcg ctgcctccac ctcgtcgcgc aatgtgcgcc tcgtcaaggg cagccacatc atcgttccga aattctggtc gggcgcaaac gcctatctcg ttcagaacca cgacaagcgc gtgatcttca tcaatccata tgaaggtgac aaggcgctga tcggcaccac cgacattgcc tatgaaggcc gggcggaaga cgttgcggcg gatgagaagg aaatcgacta tctcatcacc gcggtaaacc gctatttcaa ggaaaagctc cggcgcgaag acgtgctgca ctccttctcc ggcgtgcgcc cgctgtttga cgacggcaag ggcaatcctt ccgccgtcac ccgcgattac gtgttcgatc tggacgaaac cggcggtgca ccgctgctca acgtctttgg cggcaagatc accaccttcc gcgaactggc ggagcgcggg atgcatcggc tcaagcacat tttcccgcaa atgggcggcg actggaccca cgacgcgccg ctgcccggcg gcgagattgc caatgccgat tatgaaacct tcgccaatac cttacgcgac atctatccat ggatgccgcg cacactcgtt catcattatg gacggctcta tggtgcgcgc acaaaggatg tggtggctgg cgcgcagaac ctcgaagggc ttggccgtca cttcggcggc gatttccatg aggcggaagt gcgctatctg gtggccagag aatgggcaaa gacggcagaa gacattctct atcgccgcac caagcattac ctgcatttga ccgaagccga acgcgccgct ttcgtggaat ggttcgacaa cgccaatcta gttgcttga

Details for BrsuA.18134.a.A1.GE33826

HARVESTED ON:	10/4/2011
SEQUENCED ON:	10/7/2011
EXPECTED MW:	58kDa
OBSERVED MW:	58kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	95

Validated AA Sequence

MAEPETCDLF VIGGGINGAG VARDAAGRGL KVVLAEKDDL AQGTSSRSGK LVHGGLRYLE YYEFRLVREA LIEREVLSNA APHIIWPMRF VLPHSPQDRP AWLVRLGLFL YDHLGGRKKL PGTRTLDLKR DPEGTPILDQ YTKGFEYSDC WVDDARLVAL NAVGAAEKGA TILTRTPVVS ARRENGGWIV ETRNSDTGET RTFRARCIVN CAGPWVTDVI HNVAASTSSR NVRLVKGSHI IVPKFWSGAN AYLVQNHDKR VIFINPYEGD KALIGTTDIA YEXRAEDVAA DEKEIDYLIT AVNRYFKEKL RREDVLHSXS GVRPLFDDGK GNPSAVTRDY VFDLDETGGA PLLNVFGGKI TTFRELAERG MHRLKHIFPQ MGGDWTHDAP LPGGEIANAD YETFANTLRD IYPWMPRTLV HHYGRLYGAR TKDVVAGAQN LEGLGRHFGG DFHEAEVRYL VAREWAKTAE DILYRRTKHY LHLTEAERAA FVEWFDNANL V

Validated NT Sequence

caactagatt ggcgttgtcg aaccattcca cgaaagcggc gcgttcggct tcggtcaaat gcaggtaatg cttggtgcgg cgatagagaa tgtcttctgc cgtctttgcc cattctctgg ccaccagata gcgcacttcc gcctcatgga aatcgccgcc gaagtgacgg ccaagccctt cgaggttctg cgcgccagcc accacatcct ttgtgcgcgc accatagagc cgtccataat gatgaacgag tgtgcgcggc atccatggat agatgtcgcg taaggtattg gcgaaggttt cataatcggc attggcaatc tcgccgccgg gcagcggcgc gtcgtgggtc cagtcgccgc ccatttgcgg gaaaatgtgc ttgagccgat gcatcccgcg ctccgccagt tcgcggaagg tggtgatctt gccgccaaag acgttgagca gcggtgcacc gccggtttcg tccagatcga acacgtaatc gcgggtgacg gcggaaggat tgcccttgcc gtcgtcaaac agcgggcgca cgccgganaa ggagtgcagc acgtcttcgc gccggagctt ttccttgaaa tagcggttta ccgcggtgat gagatagtcg atttccttct catccgccgc aacgtcttcc gcccggnctt cataggcaat gtcggtggtg ccgatcagcg ccttgtcacc ttcatatgga ttgatgaaga tcacgcgctt gtcgtggttc tgaacgagat aggcgtttgc gcccgaccag aatttcggaa cgatgatgtg gctgcccttg acgaggcgca cattgcgcga cgaggtggag gcagcgacat tgtggatgac gtccgtgacc catggtccgg cgcaattgac gatgcagcgg gcgcggaagg tgcgggtttc gccggtatcg ctgtttctgg tttccacgat ccagccgcca ttttcgcggc gtgcggagac gaccggtgta cgggtgagaa tggtcgcgcc cttttcggcg gcacccaccg cattgagcgc cacgaggcgc gcatcgtcca cccagcaatc ggaatattca aaacccttgg tgtactggtc gaggatcggc gtgccttccg ggtcgcgctt cagatcgagc gtacgcgtgc cgggaagttt tttgcgcccg cccagatggt catagagaaa caggccaagc cgcaccagcc aggccgggcg atcctgcggg ctgtgcggca gcacgaagcg catcggccag atgatatggg gtgccgcatt cgaaagcact tcgcgctcga tcagcgcttc gcgcacaagg cggaactcat aatattcgag atagcgcaaa ccgccatgca ccagcttgcc ggagcgcgat gatgtgcctt gcgccagatc gtccttttct gccagcacca ccttgaggcc gcgcccggcc gcgtcgcggg ccacgcccgc accgttgatg ccgccgccaa ttacaaacag gtcgcaggtt tccggttcag ccatcgaacc aggaccctgg gtctgagctt ccagg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAEPETCDL FVIGGGINGA GVARDAAGRG LKVVLAEKDD LAQGTSSRSG KLVHGGLRYL EYYEFRLVRE ALIEREVLSN AAPHIIWPMR FVLPHSPQDR PAWLVRLGLF LYDHLGGRKK LPGTRTLDLK RDPEGTPILD QYTKGFEYSD CWVDDARLVA LNAVGAAEKG ATILTRTPVV SARRENGGWI VETRNSDTGE TRTFRARCIV NCAGPWVTDV IHNVAASTSS RNVRLVKGSH IIVPKFWSGA NAYLVQNHDK RVIFINPYEG DKALIGTTDI AYEGRAEDVA ADEKEIDYLI TAVNRYFKEK LRREDVLHSF SGVRPLFDDG KGNPSAVTRD YVFDLDETGG APLLNVFGGK ITTFRELAER GMHRLKHIFP QMGGDWTHDA PLPGGEIANA DYETFANTLR DIYPWMPRTL VHHYGRLYGA RTKDVVAGAQ NLEGLGRHFG GDFHEAEVRY LVAREWAKTA EDILYRRTKH YLHLTEAERA AFVEWFDNAN LVA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctgaaccg gaaacctgcg acctgtttgt aattggcggc ggcatcaacg gtgcgggcgt ggcccgcgac gcggccgggc gcggcctcaa ggtggtgctg gcagaaaagg acgatctggc gcaaggcaca tcatcgcgct ccggcaagct ggtgcatggc ggtttgcgct atctcgaata ttatgagttc cgccttgtgc gcgaagcgct gatcgagcgc gaagtgcttt cgaatgcggc accccatatc atctggccga tgcgcttcgt gctgccgcac agcccgcagg atcgcccggc ctggctggtg cggcttggcc tgtttctcta tgaccatctg ggcgggcgca aaaaacttcc cggcacgcgt acgctcgatc tgaagcgcga cccggaaggc acgccgatcc tcgaccagta caccaagggt tttgaatatt ccgattgctg ggtggacgat gcgcgcctcg tggcgctcaa tgcggtgggt gccgccgaaa agggcgcgac cattctcacc cgtacaccgg tcgtctccgc acgccgcgaa aatggcggct ggatcgtgga aaccagaaac agcgataccg gcgaaacccg caccttccgc gcccgctgca tcgtcaattg cgccggacca tgggtcacgg acgtcatcca caatgtcgct gcctccacct cgtcgcgcaa tgtgcgcctc gtcaagggca gccacatcat cgttccgaaa ttctggtcgg gcgcaaacgc ctatctcgtt cagaaccacg acaagcgcgt gatcttcatc aatccatatg aaggtgacaa ggcgctgatc ggcaccaccg acattgccta tgaaggccgg gcggaagacg ttgcggcgga tgagaaggaa atcgactatc tcatcaccgc ggtaaaccgc tatttcaagg aaaagctccg gcgcgaagac gtgctgcact ccttctccgg cgtgcgcccg ctgtttgacg acggcaaggg caatccttcc gccgtcaccc gcgattacgt gttcgatctg gacgaaaccg gcggtgcacc gctgctcaac gtctttggcg gcaagatcac caccttccgc gaactggcgg agcgcgggat gcatcggctc aagcacattt tcccgcaaat gggcggcgac tggacccacg acgcgccgct gcccggcggc gagattgcca atgccgatta tgaaaccttc gccaatacct tacgcgacat ctatccatgg atgccgcgca cactcgttca tcattatgga cggctctatg gtgcgcgcac aaaggatgtg gtggctggcg cgcagaacct cgaagggctt ggccgtcact tcggcggcga tttccatgag gcggaagtgc gctatctggt ggccagagaa tgggcaaaga cggcagaaga cattctctat cgccgcacca agcattacct gcatttgacc gaagccgaac gcgccgcttt cgtggaatgg ttcgacaacg ccaatctagt tgcttaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa

Details for BrsuA.18134.a.A1.PW34436

PURIFICATION DATe:	12/2/2011
CONCENTRATION:	17.61mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	50µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	95

Protocol Notes

notes unavailable

Validated AA Sequence

MAEPETCDLF VIGGGINGAG VARDAAGRGL KVVLAEKDDL AQGTSSRSGK LVHGGLRYLE YYEFRLVREA LIEREVLSNA APHIIWPMRF VLPHSPQDRP AWLVRLGLFL YDHLGGRKKL PGTRTLDLKR DPEGTPILDQ YTKGFEYSDC WVDDARLVAL NAVGAAEKGA TILTRTPVVS ARRENGGWIV ETRNSDTGET RTFRARCIVN CAGPWVTDVI HNVAASTSSR NVRLVKGSHI IVPKFWSGAN AYLVQNHDKR VIFINPYEGD KALIGTTDIA YEXRAEDVAA DEKEIDYLIT AVNRYFKEKL RREDVLHSXS GVRPLFDDGK GNPSAVTRDY VFDLDETGGA PLLNVFGGKI TTFRELAERG MHRLKHIFPQ MGGDWTHDAP LPGGEIANAD YETFANTLRD IYPWMPRTLV HHYGRLYGAR TKDVVAGAQN LEGLGRHFGG DFHEAEVRYL VAREWAKTAE DILYRRTKHY LHLTEAERAA FVEWFDNANL V

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCTGAACCG GAAACCTGCG ACCTGTTTGT AATTGGCGGC GGCATCAACG GTGCGGGCGT GGCCCGCGAC GCGGCCGGGC GCGGCCTCAA GGTGGTGCTG GCAGAAAAGG ACGATCTGGC GCAAGGCACA TCATCGCGCT CCGGCAAGCT GGTGCATGGC GGTTTGCGCT ATCTCGAATA TTATGAGTTC CGCCTTGTGC GCGAAGCGCT GATCGAGCGC GAAGTGCTTT CGAATGCGGC ACCCCATATC ATCTGGCCGA TGCGCTTCGT GCTGCCGCAC AGCCCGCAGG ATCGCCCGGC CTGGCTGGTG CGGCTTGGCC TGTTTCTCTA TGACCATCTG GGCGGGCGCA AAAAACTTCC CGGCACGCGT ACGCTCGATC TGAAGCGCGA CCCGGAAGGC ACGCCGATCC TCGACCAGTA CACCAAGGGT TTTGAATATT CCGATTGCTG GGTGGACGAT GCGCGCCTCG TGGCGCTCAA TGCGGTGGGT GCCGCCGAAA AGGGCGCGAC CATTCTCACC CGTACACCGG TCGTCTCCGC ACGCCGCGAA AATGGCGGCT GGATCGTGGA AACCAGAAAC AGCGATACCG GCGAAACCCG CACCTTCCGC GCCCGCTGCA TCGTCAATTG CGCCGGACCA TGGGTCACGG ACGTCATCCA CAATGTCGCT GCCTCCACCT CGTCGCGCAA TGTGCGCCTC GTCAAGGGCA GCCACATCAT CGTTCCGAAA TTCTGGTCGG GCGCAAACGC CTATCTCGTT CAGAACCACG ACAAGCGCGT GATCTTCATC AATCCATATG AAGGTGACAA GGCGCTGATC GGCACCACCG ACATTGCCTA TGAAGGCCGG GCGGAAGACG TTGCGGCGGA TGAGAAGGAA ATCGACTATC TCATCACCGC GGTAAACCGC TATTTCAAGG AAAAGCTCCG GCGCGAAGAC GTGCTGCACT CCTTCTCCGG CGTGCGCCCG CTGTTTGACG ACGGCAAGGG CAATCCTTCC GCCGTCACCC GCGATTACGT GTTCGATCTG GACGAAACCG GCGGTGCACC GCTGCTCAAC GTCTTTGGCG GCAAGATCAC CACCTTCCGC GAACTGGCGG AGCGCGGGAT GCATCGGCTC AAGCACATTT TCCCGCAAAT GGGCGGCGAC TGGACCCACG ACGCGCCGCT GCCCGGCGGC GAGATTGCCA ATGCCGATTA TGAAACCTTC GCCAATACCT TACGCGACAT CTATCCATGG ATGCCGCGCA CACTCGTTCA TCATTATGGA CGGCTCTATG GTGCGCGCAC AAAGGATGTG GTGGCTGGCG CGCAGAACCT CGAAGGGCTT GGCCGTCACT TCGGCGGCGA TTTCCATGAG GCGGAAGTGC GCTATCTGGT GGCCAGAGAA TGGGCAAAGA CGGCAGAAGA CATTCTCTAT CGCCGCACCA AGCATTACCT GCATTTGACC GAAGCCGAAC GCGCCGCTTT CGTGGAATGG TTCGACAACG CCAATCTAGT TGCTTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA