SSGCID: Target Page

BrsuA.18132.a

5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13)

CENTER ID:	BrsuA.18132.a
ORGANISM:	Brucella suis 1330
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuA.18132.a.A2.GE33821	domain1		1	647
BrsuA.18132.a.A3.GE33828	domain2		648	1261

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuA.18132.a.A2.PW34431	domain1		1	647

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|204722.5.peg.197
RefSeq:	NP_697226.1
UniProt:	A0A0H3G5I3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MASSLDDLFG ATAAKPDGSE VLAALTQAAR ERILILDGAM GTQIQGLGFH EEHFRGDRFA TCDCQLQGNN DLLTLTQPKA IEEIHYAYAM AGADILETNT FSSTSIAQAD YGMEAMVYDL NRDGARLARR AALRAEQKDG RRRFVAGALG PTNRTASLSP DVNNPGFRAV TFDDLRIAYS EQIRGLIDGG SDIILIETIF DTLNAKAAVF ATEEVFAEKG VRLPVMISGT ITDLSGRTLS GQTPTAFWYS LRHARPFTIG LNCALGANAM RAHLNELSGI ADTFICAYPN AGLPNEFGQY DETPEAMAAQ IEGFARDGLV NVVGGCCGST PDHIRAIAQA VAKYEPRKPA KVPPLMRLSG LEPFTLTKDI PFVNIGERTN VTGSARFRKL VKAGDFAAAL DVARDQVANG AQIIDINMDE GLIDSEKAMV EFLNLIAAEP DIARVPIMLD SSKWEVIEAG LKCVQGKAVV NSISLKEGEE AFLHHARLVR AYGAAVVIMA FDETGQADTQ ARKIEICTRA YKILTEQVGF PPEDIIFDPN IFAVATGIEE HNNYGVDFIE ATREIVRTLP HVHISGGVSN LSFSFRGNEP VREAMHAVFL YHAIQAGMDM GIVNAGQLAV YDTIDAELRE ACEDVVLNRP TKTGESATER LLEIAERFRD SGSREARTQD LSWREWPVEK RLEHALVNGI TEYIEADTEE ARLAAERPLH VIEGPLMAGM NVVGDLFGSG KMFLPQVVKS ARVMKQAVAV LLPFMEEEKR LNGGEGRQSA GKVLMATVKG DVHDIGKNIV GVVLACNNYE IIDLGVMVPS QKILQVARDE KVDIIGLSGL ITPSLDEMAH VAAEMEREGF DIPLLIGGAT TSRVHTAVKI HSRYERGQAV YVVDASRAVG VVSNLLSPEG KQAYIDGLRN EYAKVAAAHA RNEAEKQRLP IARARANPHQ LDWENYEPVK PAFTGTKVFE TYDLAEIARY IDWTPFFQTW ELRGRYPAIL EDEKQGEAAR QLWADAQAML RKIIDEKWFT PRAVVGFWPA NAVGDDIRLF TDESRKEELA TLFTLRQQLT KRDGRPNVAM ADFVAPVESG KQDYVGGFVV TAGIGEIAIT ERFERANDDY SAILVKALAD RFAEAFAELM HERVRKEFWA YAPDEAFTPE ELISEPYKGI RPAPGYPAQP DHTEKTTLFR LLDATANTGV ELTESYAMWP GSSVSGLYIG HPESYYFGVA KVERDQVEDY ARRKDMDVEA VERWLTPILN YVPGASKDEA A

NT Sequence

atggcgtctt cccttgacga tctttttggt gcaacggcgg caaagccgga cggttcggaa gtgctggcgg cactgaccca ggcggcgcgc gagcgcatcc tgatcctcga cggcgccatg ggtacgcaga ttcagggcct gggtttccat gaagagcatt ttcgcggcga tcgtttcgcc acctgtgact gccagcttca gggcaataac gatcttctga ccttgaccca gccgaaagcc attgaggaaa tccactatgc ctatgccatg gcgggcgcgg atattctcga aaccaatact ttctcctcca cttccatcgc gcaggccgat tatggcatgg aggcgatggt ctatgatctc aatcgcgatg gcgcacgcct tgcccgccgc gcagccttgc gcgccgagca gaaggacggc cgccgccgct ttgtcgcggg cgcactgggg ccgacgaacc gcaccgcctc gctgtccccg gatgtgaaca atccgggctt tcgcgccgtc actttcgatg atctgcgcat tgcctattcc gaacagattc gcggcctgat cgatggcggt tccgacatca tcctgatcga aaccattttc gatacgctga acgccaaggc ggctgtgttc gcgaccgagg aagtctttgc cgaaaagggt gttcgccttc cggtcatgat ctccggtacg atcaccgatc tttccgggcg cacgctttcc ggccagacgc cgacggcctt ctggtattca ctgcgccatg caagaccgtt caccatcgga ctgaattgcg cccttggcgc caatgccatg cgtgcgcatc tgaatgaatt gtcgggcatt gccgacacct ttatctgcgc ctatcccaat gccggccttc cgaacgaatt cggccagtat gatgaaacgc cggaagccat ggccgcgcag atcgagggct ttgctcgcga cgggctggtc aatgttgttg gcggctgctg cggctccacg cctgaccata ttcgcgccat cgcgcaagcc gtcgcgaaat atgagccacg caagcccgca aaagtgccgc cgctgatgcg cctttccggc cttgagccct ttacgctcac caaggatatt cctttcgtca atatcggcga gcgcaccaat gtcaccggct cggcccgctt ccgcaagctc gtcaaggccg gcgatttcgc ggctgcgctc gatgttgcgc gcgatcaggt ggccaacggc gcccagatca tcgatatcaa catggatgaa ggcctgatcg attccgaaaa ggccatggtc gagtttttga accttattgc cgccgagccg gatatcgccc gcgtgccgat catgctcgac agctccaaat gggaagtgat cgaggctggc ctgaaatgcg ttcagggcaa ggcagtcgtc aattccatct cgctgaaaga gggtgaggaa gcctttcttc accatgcgcg gctggtgcgt gcctatggtg ccgctgtggt catcatggcg ttcgatgaaa ccggacaggc ggatacgcag gcccgcaaga tcgaaatctg cacccgcgcc tataaaatcc tgaccgaaca ggtgggtttc ccgccggaag acatcatttt cgatccgaat atttttgcgg tcgcgaccgg catcgaggaa cacaataatt acggcgtcga tttcatcgag gccacgcgtg aaatcgtccg cacactgccg catgtccata tttccggcgg cgtttccaac ctctcgttct ccttccgcgg caatgagccg gtgcgtgagg cgatgcatgc cgttttcctc taccacgcca ttcaggcggg catggatatg ggcatcgtca atgcgggcca gcttgcggtc tatgacacga tcgacgctga actgcgtgag gcttgcgagg atgtggtgct gaaccgcccc acaaaaacgg gcgaaagcgc aaccgaacgg cttctggaaa ttgccgaacg gttccgtgat tccggctcca gggaagcgcg gacgcaggat ttgagctggc gcgaatggcc ggtcgaaaag cggcttgaac atgcgctggt caacggcatc accgaatata tcgaggccga tacggaagag gcgcgccttg ctgccgagcg cccgttgcat gtgatcgaag ggccgctgat ggcaggcatg aatgtggtcg gcgacctgtt cggttccggc aagatgtttc tgccgcaggt ggtgaaatct gcccgcgtga tgaagcaggc cgtggccgtg ctgctgccct tcatggaaga ggaaaagcgc ctcaatggcg gcgagggccg ccagagtgcg ggcaaggtgc tgatggcgac cgtcaagggc gatgtgcacg atatcggcaa gaatatcgtg ggcgtggttc tggcctgcaa taattatgaa atcatcgatc ttggcgtcat ggtgccctcg cagaaaatcc tgcaggtggc gcgcgatgaa aaggtggata tcatcggcct ttccggcctc atcacgcctt cgcttgatga gatggcgcat gtcgccgccg aaatggagcg cgagggcttc gatattccgc ttttgattgg cggtgcgacg acgagccgtg ttcatacggc ggtgaaaatc cattcgcgtt acgagcgcgg gcaggctgtc tatgtcgtgg atgcaagtcg tgcggtgggg gtcgtgtcaa acctcctgtc gccggagggc aagcaggcct atatcgacgg tttacgcaac gaatatgcca aggtggctgc tgcccatgcc cgcaacgagg ctgaaaaaca gcgcctgcca attgcccgcg cgcgcgccaa tccgcatcag ctcgactggg aaaactacga gccggtgaag ccggccttca ccggaacgaa ggtttttgaa acctatgatc tggcggaaat cgcgcgctat atcgactgga ccccgttctt ccagacctgg gaactgaggg gccgctatcc ggctattctc gaagatgaaa agcagggtga ggcggcgcgc cagctctggg cggatgcgca agcgatgctc cggaagatca tcgatgagaa atggttcacc ccgcgggcgg tggtcggctt ctggcctgcc aatgcggtgg gcgatgatat ccgcctcttt acggatgaaa gccgcaagga ggagcttgcc accctgttca cgcttcgcca gcagcttacc aagcgcgatg gccgcccgaa tgtcgccatg gcggattttg tggcgcctgt cgaaagcggc aaacaggatt atgttggcgg ttttgtcgtg acggcgggca ttggcgagat tgctatcaca gagcgttttg aacgcgccaa tgatgattat tccgccattc ttgtgaaggc gcttgccgat cgtttcgcgg aagccttcgc ggaactgatg catgagcgtg tgcgcaagga attctgggcc tatgcgccgg atgaagcctt tacgccggaa gaactgatca gcgagccata taagggcatc cgcccggcgc cgggctatcc ggcccagccc gatcatacgg aaaagaccac cttgttccgc ctgctcgatg ccaccgccaa taccggcgtg gagcttaccg aaagctacgc catgtggccc ggctcctcgg tgtcgggtct ctatatcggt caccctgaaa gctattattt cggtgtggca aaggtggagc gcgatcaggt cgaggattat gcccggcgca aggacatgga tgtggaggct gtggagcgct ggctcacccc gatcctcaat tatgtccctg gggcatcgaa agacgaagcg gcctga

Details for BrsuA.18132.a.A2.GE33821

HARVESTED ON:	10/4/2011
SEQUENCED ON:	10/7/2011
EXPECTED MW:	72kDa
OBSERVED MW:	72kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	96
PERCENT COVERAGE:	48

Validated AA Sequence

Validated NT Sequence

ttgtnnnntt taagaaggag atataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atggcgtctt cccttgacga tctttttggt gcaacggcgg caaagccgga cggttcggaa gtgctggcgg cactgaccca ggcggcgcgc gagcgcatcc tgatcctcga cggcgccatg ggtacgcaga ttcagggcct gggtttccat gaagagcatt ttcgcggcga tcgtttcgcc acctgtgact gccagcttca gggcaataac gatcttctga ccttgaccca gccgaaagcc attgaggaaa tccactatgc ctatgccatg gcgggcgcgg atattctcga aaccaatact ttctcctcca cttccatcgc gcaggccgat tatggcatgg aggcgatggt ctatgatctc aatcgcgatg gcgcacgcct tgcccgccgc gcagccttgc gcgccgagca gaaggacggc cgccgccgct ttgtcgcggg cgcactgggg ccgacgaacc gcaccgcctc gctgtccccg gatgtgaaca atccgggctt tcgcgccgtc actttcgatg atctgcgcat tgcctattcc gaacagattc gcggcctgat cgatggcggt tccgacatca tcctgatcga aaccattttc gatacgctga acgccaaggc ggctgtgttc gcgaccgang aagtctttgc cgaaaagggt gttcgccttc cggtcatgat ctccggtacg atcaccgatc tttccgggcg cacgctttcc ggccagacgc cgacggcctt ctggtattca ctgcgccatg caagaccgtt caccatcgga ctgaattgcg cccttggcgc caatgccatg cgtgcgcatc tgaatgaatt gtcgggcatt gccgacacct ttatctgcgc ctatcccnat gncnggcctt ncgaacgaan tcgnccagta tgatgaaacg ccggaagcca tgnnnnncan atcgagggct tngctcnnga nnggntngnc nntnnntnnn ntgcngnnnn ncnncntntn ncnnntcnnn nnannnnnnn naannntgan cnnnnnnnnn nccncaaann nnnnnnnnna ngnnnnnnnn nnnnnnnnnn nnnnnncnnn gnnnnnnnnn nannnnnnna

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMASSLDDLF GATAAKPDGS EVLAALTQAA RERILILDGA MGTQIQGLGF HEEHFRGDRF ATCDCQLQGN NDLLTLTQPK AIEEIHYAYA MAGADILETN TFSSTSIAQA DYGMEAMVYD LNRDGARLAR RAALRAEQKD GRRRFVAGAL GPTNRTASLS PDVNNPGFRA VTFDDLRIAY SEQIRGLIDG GSDIILIETI FDTLNAKAAV FATEEVFAEK GVRLPVMISG TITDLSGRTL SGQTPTAFWY SLRHARPFTI GLNCALGANA MRAHLNELSG IADTFICAYP NAGLPNEFGQ YDETPEAMAA QIEGFARDGL VNVVGGCCGS TPDHIRAIAQ AVAKYEPRKP AKVPPLMRLS GLEPFTLTKD IPFVNIGERT NVTGSARFRK LVKAGDFAAA LDVARDQVAN GAQIIDINMD EGLIDSEKAM VEFLNLIAAE PDIARVPIML DSSKWEVIEA GLKCVQGKAV VNSISLKEGE EAFLHHARLV RAYGAAVVIM AFDETGQADT QARKIEICTR AYKILTEQVG FPPEDIIFDP NIFAVATGIE EHNNYGVDFI EATREIVRTL PHVHISGGVS NLSFSFRGNE PVREAMHAVF LYHAIQAGMD MGIVNAGQLA VYDTIDAELR EACEDVVLNR PTKTGESA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgtcttcc cttgacgatc tttttggtgc aacggcggca aagccggacg gttcggaagt gctggcggca ctgacccagg cggcgcgcga gcgcatcctg atcctcgacg gcgccatggg tacgcagatt cagggcctgg gtttccatga agagcatttt cgcggcgatc gtttcgccac ctgtgactgc cagcttcagg gcaataacga tcttctgacc ttgacccagc cgaaagccat tgaggaaatc cactatgcct atgccatggc gggcgcggat attctcgaaa ccaatacttt ctcctccact tccatcgcgc aggccgatta tggcatggag gcgatggtct atgatctcaa tcgcgatggc gcacgccttg cccgccgcgc agccttgcgc gccgagcaga aggacggccg ccgccgcttt gtcgcgggcg cactggggcc gacgaaccgc accgcctcgc tgtccccgga tgtgaacaat ccgggctttc gcgccgtcac tttcgatgat ctgcgcattg cctattccga acagattcgc ggcctgatcg atggcggttc cgacatcatc ctgatcgaaa ccattttcga tacgctgaac gccaaggcgg ctgtgttcgc gaccgaggaa gtctttgccg aaaagggtgt tcgccttccg gtcatgatct ccggtacgat caccgatctt tccgggcgca cgctttccgg ccagacgccg acggccttct ggtattcact gcgccatgca agaccgttca ccatcggact gaattgcgcc cttggcgcca atgccatgcg tgcgcatctg aatgaattgt cgggcattgc cgacaccttt atctgcgcct atcccaatgc cggccttccg aacgaattcg gccagtatga tgaaacgccg gaagccatgg ccgcgcagat cgagggcttt gctcgcgacg ggctggtcaa tgttgttggc ggctgctgcg gctccacgcc tgaccatatt cgcgccatcg cgcaagccgt cgcgaaatat gagccacgca agcccgcaaa agtgccgccg ctgatgcgcc tttccggcct tgagcccttt acgctcacca aggatattcc tttcgtcaat atcggcgagc gcaccaatgt caccggctcg gcccgcttcc gcaagctcgt caaggccggc gatttcgcgg ctgcgctcga tgttgcgcgc gatcaggtgg ccaacggcgc ccagatcatc gatatcaaca tggatgaagg cctgatcgat tccgaaaagg ccatggtcga gtttttgaac cttattgccg ccgagccgga tatcgcccgc gtgccgatca tgctcgacag ctccaaatgg gaagtgatcg aggctggcct gaaatgcgtt cagggcaagg cagtcgtcaa ttccatctcg ctgaaagagg gtgaggaagc ctttcttcac catgcgcggc tggtgcgtgc ctatggtgcc gctgtggtca tcatggcgtt cgatgaaacc ggacaggcgg atacgcaggc ccgcaagatc gaaatctgca cccgcgccta taaaatcctg accgaacagg tgggtttccc gccggaagac atcattttcg atccgaatat ttttgcggtc gcgaccggca tcgaggaaca caataattac ggcgtcgatt tcatcgaggc cacgcgtgaa atcgtccgca cactgccgca tgtccatatt tccggcggcg tttccaacct ctcgttctcc ttccgcggca atgagccggt gcgtgaggcg atgcatgccg ttttcctcta ccacgccatt caggcgggca tggatatggg catcgtcaat gcgggccagc ttgcggtcta tgacacgatc gacgctgaac tgcgtgaggc ttgcgaggat gtggtgctga accgccccac aaaaacgggc gaaagcgcat aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa

Details for BrsuA.18132.a.A3.GE33828

HARVESTED ON:	10/4/2011
SEQUENCED ON:	10/7/2011
EXPECTED MW:	71kDa
OBSERVED MW:	71kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	84
PERCENT COVERAGE:	59

Validated AA Sequence

Validated NT Sequence

attttgttta ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaccg aacggcttct ggaaattgcc gaacggttcc gtgattccgg ctccagggaa gcgcggacgc aggatttgag ctggcgcgaa tggccggtcg aaaagcggct tgaacatgcg ctggtcaacg gcatcaccga atatatcgag gccgatacgg aagaggcgcg ccttgctgcc gagcgcccgt tgcatgtgat cgaagggccg ctgatggcag gcatgaatgt ggtcggcgac ctgttcggtt ccggcaagat gtttctgccg caggtggtga aatctgcccg cgtgatgaag caggccgtgg ccgtgctgct gcccttcatg gaagaggaaa agcgcctcaa tggcggcgag ggccgccaga gtgcgggcaa ggtgctgatg gcgaccgtca agggcgatgt gcacgatatc ggcaagaata tcgtgggcgt ggttctggcc tgcaataatt atgaaatcat cgatcttggc gtcatggtgc cctcgcagaa aatcctgcag gtggcgcgcg atgaaaaggt ggatatcatc ggcctttccg gcctcatcac gccttcgctt gatgagatgg cgcatgtcgc cgccgaaatg gagcgcgagg gcttcgatat tccgcttttg attggcggtg cgacgacgag ccgtgttcat acggcggtga aaatccattc gcgttacgag cgcgggcagg ctgtctatgt cgtgnatgca agtcgtgcgg tgggggtcgt gtcaaacctc ctgtcgccng agggcaagca ngcctatatc gacggtttac gcaacgaata tgccnaggtg gctgctgccc atgccngcaa cgaggctgaa aaacagcgcc tgccaattgc ccgcgcgcgc gccaatccgc atcagctcga ctgggaaanc tacnancngg tgaannnnnn nnnnnnnnna gttttgaaaa tgatctngnn nancgcnnnc nnnncgacng nnccnnnnnt nnnnnnnnnn nnnntcgnnt nntnnnnnan annnaannnn nnnnnnnnnn nnnnnnnnnn nnnncnnnan nnnnnnnnnn nanntnnncc ccc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMTERLLEIA ERFRDSGSRE ARTQDLSWRE WPVEKRLEHA LVNGITEYIE ADTEEARLAA ERPLHVIEGP LMAGMNVVGD LFGSGKMFLP QVVKSARVMK QAVAVLLPFM EEEKRLNGGE GRQSAGKVLM ATVKGDVHDI GKNIVGVVLA CNNYEIIDLG VMVPSQKILQ VARDEKVDII GLSGLITPSL DEMAHVAAEM EREGFDIPLL IGGATTSRVH TAVKIHSRYE RGQAVYVVDA SRAVGVVSNL LSPEGKQAYI DGLRNEYAKV AAAHARNEAE KQRLPIARAR ANPHQLDWEN YEPVKPAFTG TKVFETYDLA EIARYIDWTP FFQTWELRGR YPAILEDEKQ GEAARQLWAD AQAMLRKIID EKWFTPRAVV GFWPANAVGD DIRLFTDESR KEELATLFTL RQQLTKRDGR PNVAMADFVA PVESGKQDYV GGFVVTAGIG EIAITERFER ANDDYSAILV KALADRFAEA FAELMHERVR KEFWAYAPDE AFTPEELISE PYKGIRPAPG YPAQPDHTEK TTLFRLLDAT ANTGVELTES YAMWPGSSVS GLYIGHPESY YFGVAKVERD QVEDYARRKD MDVEAVERWL TPILNYVPGA SKDEAA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgaacgg cttctggaaa ttgccgaacg gttccgtgat tccggctcca gggaagcgcg gacgcaggat ttgagctggc gcgaatggcc ggtcgaaaag cggcttgaac atgcgctggt caacggcatc accgaatata tcgaggccga tacggaagag gcgcgccttg ctgccgagcg cccgttgcat gtgatcgaag ggccgctgat ggcaggcatg aatgtggtcg gcgacctgtt cggttccggc aagatgtttc tgccgcaggt ggtgaaatct gcccgcgtga tgaagcaggc cgtggccgtg ctgctgccct tcatggaaga ggaaaagcgc ctcaatggcg gcgagggccg ccagagtgcg ggcaaggtgc tgatggcgac cgtcaagggc gatgtgcacg atatcggcaa gaatatcgtg ggcgtggttc tggcctgcaa taattatgaa atcatcgatc ttggcgtcat ggtgccctcg cagaaaatcc tgcaggtggc gcgcgatgaa aaggtggata tcatcggcct ttccggcctc atcacgcctt cgcttgatga gatggcgcat gtcgccgccg aaatggagcg cgagggcttc gatattccgc ttttgattgg cggtgcgacg acgagccgtg ttcatacggc ggtgaaaatc cattcgcgtt acgagcgcgg gcaggctgtc tatgtcgtgg atgcaagtcg tgcggtgggg gtcgtgtcaa acctcctgtc gccggagggc aagcaggcct atatcgacgg tttacgcaac gaatatgcca aggtggctgc tgcccatgcc cgcaacgagg ctgaaaaaca gcgcctgcca attgcccgcg cgcgcgccaa tccgcatcag ctcgactggg aaaactacga gccggtgaag ccggccttca ccggaacgaa ggtttttgaa acctatgatc tggcggaaat cgcgcgctat atcgactgga ccccgttctt ccagacctgg gaactgaggg gccgctatcc ggctattctc gaagatgaaa agcagggtga ggcggcgcgc cagctctggg cggatgcgca agcgatgctc cggaagatca tcgatgagaa atggttcacc ccgcgggcgg tggtcggctt ctggcctgcc aatgcggtgg gcgatgatat ccgcctcttt acggatgaaa gccgcaagga ggagcttgcc accctgttca cgcttcgcca gcagcttacc aagcgcgatg gccgcccgaa tgtcgccatg gcggattttg tggcgcctgt cgaaagcggc aaacaggatt atgttggcgg ttttgtcgtg acggcgggca ttggcgagat tgctatcaca gagcgttttg aacgcgccaa tgatgattat tccgccattc ttgtgaaggc gcttgccgat cgtttcgcgg aagccttcgc ggaactgatg catgagcgtg tgcgcaagga attctgggcc tatgcgccgg atgaagcctt tacgccggaa gaactgatca gcgagccata taagggcatc cgcccggcgc cgggctatcc ggcccagccc gatcatacgg aaaagaccac cttgttccgc ctgctcgatg ccaccgccaa taccggcgtg gagcttaccg aaagctacgc catgtggccc ggctcctcgg tgtcgggtct ctatatcggt caccctgaaa gctattattt cggtgtggca aaggtggagc gcgatcaggt cgaggattat gcccggcgca aggacatgga tgtggaggct gtggagcgct ggctcacccc gatcctcaat tatgtccctg gggcatcgaa agacgaagcg gcctaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa

Details for BrsuA.18132.a.A2.PW34431

PURIFICATION DATe:	2/5/2012
CONCENTRATION:	39.31mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	110µl
PERCENT IDENTITY:	96
PERCENT COVERAGE:	48

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGTCTTCC CTTGACGATC TTTTTGGTGC AACGGCGGCA AAGCCGGACG GTTCGGAAGT GCTGGCGGCA CTGACCCAGG CGGCGCGCGA GCGCATCCTG ATCCTCGACG GCGCCATGGG TACGCAGATT CAGGGCCTGG GTTTCCATGA AGAGCATTTT CGCGGCGATC GTTTCGCCAC CTGTGACTGC CAGCTTCAGG GCAATAACGA TCTTCTGACC TTGACCCAGC CGAAAGCCAT TGAGGAAATC CACTATGCCT ATGCCATGGC GGGCGCGGAT ATTCTCGAAA CCAATACTTT CTCCTCCACT TCCATCGCGC AGGCCGATTA TGGCATGGAG GCGATGGTCT ATGATCTCAA TCGCGATGGC GCACGCCTTG CCCGCCGCGC AGCCTTGCGC GCCGAGCAGA AGGACGGCCG CCGCCGCTTT GTCGCGGGCG CACTGGGGCC GACGAACCGC ACCGCCTCGC TGTCCCCGGA TGTGAACAAT CCGGGCTTTC GCGCCGTCAC TTTCGATGAT CTGCGCATTG CCTATTCCGA ACAGATTCGC GGCCTGATCG ATGGCGGTTC CGACATCATC CTGATCGAAA CCATTTTCGA TACGCTGAAC GCCAAGGCGG CTGTGTTCGC GACCGAGGAA GTCTTTGCCG AAAAGGGTGT TCGCCTTCCG GTCATGATCT CCGGTACGAT CACCGATCTT TCCGGGCGCA CGCTTTCCGG CCAGACGCCG ACGGCCTTCT GGTATTCACT GCGCCATGCA AGACCGTTCA CCATCGGACT GAATTGCGCC CTTGGCGCCA ATGCCATGCG TGCGCATCTG AATGAATTGT CGGGCATTGC CGACACCTTT ATCTGCGCCT ATCCCAATGC CGGCCTTCCG AACGAATTCG GCCAGTATGA TGAAACGCCG GAAGCCATGG CCGCGCAGAT CGAGGGCTTT GCTCGCGACG GGCTGGTCAA TGTTGTTGGC GGCTGCTGCG GCTCCACGCC TGACCATATT CGCGCCATCG CGCAAGCCGT CGCGAAATAT GAGCCACGCA AGCCCGCAAA AGTGCCGCCG CTGATGCGCC TTTCCGGCCT TGAGCCCTTT ACGCTCACCA AGGATATTCC TTTCGTCAAT ATCGGCGAGC GCACCAATGT CACCGGCTCG GCCCGCTTCC GCAAGCTCGT CAAGGCCGGC GATTTCGCGG CTGCGCTCGA TGTTGCGCGC GATCAGGTGG CCAACGGCGC CCAGATCATC GATATCAACA TGGATGAAGG CCTGATCGAT TCCGAAAAGG CCATGGTCGA GTTTTTGAAC CTTATTGCCG CCGAGCCGGA TATCGCCCGC GTGCCGATCA TGCTCGACAG CTCCAAATGG GAAGTGATCG AGGCTGGCCT GAAATGCGTT CAGGGCAAGG CAGTCGTCAA TTCCATCTCG CTGAAAGAGG GTGAGGAAGC CTTTCTTCAC CATGCGCGGC TGGTGCGTGC CTATGGTGCC GCTGTGGTCA TCATGGCGTT CGATGAAACC GGACAGGCGG ATACGCAGGC CCGCAAGATC GAAATCTGCA CCCGCGCCTA TAAAATCCTG ACCGAACAGG TGGGTTTCCC GCCGGAAGAC ATCATTTTCG ATCCGAATAT TTTTGCGGTC GCGACCGGCA TCGAGGAACA CAATAATTAC GGCGTCGATT TCATCGAGGC CACGCGTGAA ATCGTCCGCA CACTGCCGCA TGTCCATATT TCCGGCGGCG TTTCCAACCT CTCGTTCTCC TTCCGCGGCA ATGAGCCGGT GCGTGAGGCG ATGCATGCCG TTTTCCTCTA CCACGCCATT CAGGCGGGCA TGGATATGGG CATCGTCAAT GCGGGCCAGC TTGCGGTCTA TGACACGATC GACGCTGAAC TGCGTGAGGC TTGCGAGGAT GTGGTGCTGA ACCGCCCCAC AAAAACGGGC GAAAGCGCAT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA