BrsuA.18130.a

Phosphoenolpyruvate-protein phosphotransferase (EC 2.7.3.9)

CENTER ID: BrsuA.18130.a
ORGANISM: Brucella suis 1330
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuA.18130.a.A1.GE33829 full length 1 756
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuA.18130.a.A1.PW34439 full length 1 756

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|204722.5.peg.1905
RefSeq: NP_698850.1
UniProt: A0A0H3G4L5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRELTTGPRV LLKRLRELMA EPLEPQARLD RIVREIAQNM VAEVCSFYIL RADGVLELYA TEGLNPQAVH LAQLRLGQGL VGTIAASARP LNLTDAQSHP AFAYLPETGE EAYNSFLGVP VLRAGRTLGV LVVQNRTKRA YREDEVEALE TTAMVLAEII ATGDGLRLAR PGIELDLGRP MSVSGHGFND GIGLGHVVLH EPRIVVTNLF NEDSQAELNR LDEALGSLRI SIDDMLSRRD VAVEGEHREV LEAYRMFAHD RGWVRRLEEA VHNGLTAEAA VEKVQSDTRA RMVHLTDPYM RERLSDFDDL ANRLLRQLMG RDVKTIAESL AKDAIIVARS MGAAELLDYP RERLRGVVLE DGAATSHVVI VARAMGIPVV GQAKGVVSMA ENNDAIIVDG DEGIVHLRPQ ADVESAYAEK VRFRARRQAH YRELRDKPAI TKDGVDIALL MNAGLLVDLP QLSASGAAGI GLFRTELQFM VASTFPRSEQ QERLYRSVID AAGDKPVTFR TLDIGGDKVL PYFRATAHEE NPALGWRAIR LTLDRPGLLR TQLRALLKAA GGRELKLMLP MVTEVSEVKA AREIIEREVR HLSRFAHSLP LCLKLGAMVE VPALLWQLDE LMTAVDFVSV GSNDLFQFLM AADRGNSLIS DRFDQLSPSF LRALRHIVKA GARHEKPVTL CGEMAGRPLT AMTLIGLGFR SISMSAAAIG PVKAMLGALD AGKLNALLLE ELNKPNNAHS LRELLLRFAE DNEIPL
NT Sequence
atgcgtgagc tgacaaccgg tccccgtgta ctgctcaagc ggttgcgtga attgatggcg gagccgctgg aaccacaggc gcgccttgac aggatcgtcc gcgaaattgc gcagaacatg gtcgcggaag tctgctcctt ctatatattg cgcgcagacg gcgttcttga actttacgcc accgaaggtc ttaatcccca agctgtccac cttgcccagc ttcgtctggg gcagggcctt gtgggcacca ttgcggcaag cgcacgcccg ctcaacctga cggatgcgca gagccatccg gctttcgcct atctgccgga aacgggcgag gaagcctata attccttcct cggtgtgcct gtgcttcgcg ctggccgcac tttaggcgtt cttgtcgtgc agaatcgcac caaacgcgcc tatcgagaag acgaggttga agcacttgag acaaccgcca tggttctcgc cgaaatcatc gcaaccgggg atggtcttcg tctggcgcgt ccaggaatcg aactggatct cgggcggccg atgagcgtaa gcggccacgg tttcaacgat ggtatcgggc ttggccatgt ggtgctgcat gagccgcgca tcgtcgtgac caatcttttc aatgaggaca gtcaggccga actcaaccgg ctggacgagg cgctcggctc cttgcgtatt tccatcgatg acatgttgtc gcgccgcgat gtggcggtgg agggcgagca tcgcgaggtg ctggaagcct atcgcatgtt cgcgcatgat cgcggctggg tgcgtcgtct ggaagaagcg gttcataacg gccttacggc agaagctgcc gtggaaaagg tgcagagcga cacgcgcgcg cgcatggtgc atctgaccga cccctatatg cgcgagcgcc tttcggattt cgacgatctc gccaatcgcc tcctgcgcca gcttatgggc cgcgacgtca agacgattgc cgaatcactg gccaaggatg cgatcatcgt tgcccgttcc atgggcgcgg ctgagcttct ggactatccg cgcgaacgcc tgcgcggtgt cgtgctggaa gacggggcgg ccaccagcca tgtggtgatc gtggcgcgcg ccatgggtat tcccgtcgtc gggcaggcca agggtgttgt ttccatggcg gaaaacaacg atgcgatcat cgtcgatggc gatgagggca tcgtgcatct gcgcccgcag gccgatgtgg aatccgccta tgcggaaaag gtccgcttcc gtgcacggcg gcaggcgcat taccgtgaat tgcgcgacaa gcccgccatc acgaaggatg gcgttgatat cgcgcttttg atgaatgccg gcttgcttgt cgatctgccg cagctttccg cttcgggcgc tgccggcatt ggcctgtttc gcaccgagct tcaattcatg gtggcttcca ccttcccgcg ctcggaacag caggagcggc tctaccgttc ggtgatcgat gccgcaggtg acaagcccgt cacgttccgc acgctggata ttggtggcga caaggttctc ccctatttcc gcgcaactgc gcatgaggaa aacccggcgc tcggctggcg cgccattcgt ttgacgctcg accgtcccgg cctgttgcgc acccagttgc gcgcgctatt gaaggcagcg ggtggccgtg aactgaagct catgctgccc atggtgacgg aagtgagcga agtgaaggcc gcgcgcgaga tcatcgaacg cgaggttcgc catctttccc gctttgcgca ttcgctgccg ctgtgtctca agcttggcgc gatggtggaa gtgccggcgc tattgtggca gctcgacgaa ttgatgacgg cggtggattt cgtttcggtg ggctccaacg acctgttcca gttcctgatg gcggcggatc gcggcaattc gctgatttcg gaccgttttg accagctttc accatcgttc ctgcgtgcgc tgcgtcatat cgtgaaagcg ggggcgcggc atgaaaagcc ggtgacgctt tgcggtgaaa tggcaggccg ccccttgacg gcaatgacgc tgatcgggct tggcttccgt tcgatttcca tgtcggcggc ggcgatcggc ccggtcaagg ccatgctcgg cgcacttgat gcgggcaagc tcaatgcgct tcttctggaa gagctcaaca agccgaacaa tgcgcattcc ttgcgcgaat tgttgcttcg atttgccgaa gacaacgaaa ttccgttata g
Details for BrsuA.18130.a.A1.GE33829
HARVESTED ON: 10/4/2011
SEQUENCED ON: 10/7/2011
EXPECTED MW: 85kDa
OBSERVED MW: 85kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 83
PERCENT COVERAGE: 50
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRELTTGPR VLLKRLRELM AEPLEPQARL DRIVREIAQN MVAEVCSFYI LRADGVLELY ATEGLNPQAV HLAQLRLGQG LVGTIAASAR PLNLTDAQSH PAFAYLPETG EEAYNSFLGV PVLRAGRTLG VLVVQNRTKR AYREDEVEAL ETTAMVLAEI IATGDGLRLA RPGIELDLGR PMSVSGHGFN DGIGLGHVVL HEPRIVVTNL FNEDSQAELN RLDEALGSLR ISIDDMLSRR DVAVEGEHRE VLEAYRMFAH DRGWVRRLEE AVHNGLTAEX AVXKXQSDTR ARMVHLTTPI CASAFXISTI SXSXXXLMGX DXXRXXXXXA IIXPXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXX
Validated NT Sequence
tttgtnnnnt ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgcgtgag ctgacaaccg gtccccgtgt actgctcaag cggttgcgtg aattgatggc ggagccgctg gaaccacagg cgcgccttga caggatcgtc cgcgaaattg cgcagaacat ggtcgcggaa gtctgctcct tctatatatt gcgcgcagac ggcgttcttg aactttacgc caccgaaggt cttaatcccc aagctgtcca ccttgcccag cttcgtctgg ggcagggcct tgtgggcacc attgcggcaa gcgcacgccc gctcaacctg acggatgcgc agagccatcc ggctttcgcc tatctgccgg aaacgggcga ggaagcctat aattccttcc tcggtgtgcc tgtgcttcgc gctggccgca ctttaggcgt tcttgtcgtg cagaatcgca ccaaacgcgc ctatcgagaa gacgaggttg aagcacttga gacaaccgcc atggttctcg ccgaaatcat cgcaaccggg gatggtcttc gtctggcgcg tccaggaatc gaactggatc tcgggcggcc gatgagcgta agcggccacg gtttcaacga tggtatcggg cttggccatg tggtgctgca tgagccgcgc atcgtcgtga ccaatctttt caatgaggac agtcaggccg aactcaaccg gctggacgag gcgctcggct ccttgcgtat ttccatcgat gacatgttgt cgcgccgcga tgtggcggtg gagggcgagc atcgcgaggt gctggaagcc tatcgcatgt tcgcgcatga tcgcggctgg gtgcgtcgtc tggaagaagc ggttcataac ggccttacgg cagaanctgc cgtggnaaag gngcagagcg acacgcgcgc gcgcatggtg catctgacga cccctatatg cgcgagcgcc tttnngattt cgacgatctc nnnntcnncn ncncnnctta tgggnnnnga cntnanncga nngncnannn ntnnngcnat catcnnnccn nnnnggnnnn nnnntgannt nnnnntancc nnnannnnnn nnnnnnnnnn nnnnnnngnn nnnnnnnnnn nnnngnnnnn nnncnngnnn nnnnnnnnnn nnnnng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRELTTGPR VLLKRLRELM AEPLEPQARL DRIVREIAQN MVAEVCSFYI LRADGVLELY ATEGLNPQAV HLAQLRLGQG LVGTIAASAR PLNLTDAQSH PAFAYLPETG EEAYNSFLGV PVLRAGRTLG VLVVQNRTKR AYREDEVEAL ETTAMVLAEI IATGDGLRLA RPGIELDLGR PMSVSGHGFN DGIGLGHVVL HEPRIVVTNL FNEDSQAELN RLDEALGSLR ISIDDMLSRR DVAVEGEHRE VLEAYRMFAH DRGWVRRLEE AVHNGLTAEA AVEKVQSDTR ARMVHLTDPY MRERLSDFDD LANRLLRQLM GRDVKTIAES LAKDAIIVAR SMGAAELLDY PRERLRGVVL EDGAATSHVV IVARAMGIPV VGQAKGVVSM AENNDAIIVD GDEGIVHLRP QADVESAYAE KVRFRARRQA HYRELRDKPA ITKDGVDIAL LMNAGLLVDL PQLSASGAAG IGLFRTELQF MVASTFPRSE QQERLYRSVI DAAGDKPVTF RTLDIGGDKV LPYFRATAHE ENPALGWRAI RLTLDRPGLL RTQLRALLKA AGGRELKLML PMVTEVSEVK AAREIIEREV RHLSRFAHSL PLCLKLGAMV EVPALLWQLD ELMTAVDFVS VGSNDLFQFL MAADRGNSLI SDRFDQLSPS FLRALRHIVK AGARHEKPVT LCGEMAGRPL TAMTLIGLGF RSISMSAAAI GPVKAMLGAL DAGKLNALLL EELNKPNNAH SLRELLLRFA EDNEIPL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgtgagctg acaaccggtc cccgtgtact gctcaagcgg ttgcgtgaat tgatggcgga gccgctggaa ccacaggcgc gccttgacag gatcgtccgc gaaattgcgc agaacatggt cgcggaagtc tgctccttct atatattgcg cgcagacggc gttcttgaac tttacgccac cgaaggtctt aatccccaag ctgtccacct tgcccagctt cgtctggggc agggccttgt gggcaccatt gcggcaagcg cacgcccgct caacctgacg gatgcgcaga gccatccggc tttcgcctat ctgccggaaa cgggcgagga agcctataat tccttcctcg gtgtgcctgt gcttcgcgct ggccgcactt taggcgttct tgtcgtgcag aatcgcacca aacgcgccta tcgagaagac gaggttgaag cacttgagac aaccgccatg gttctcgccg aaatcatcgc aaccggggat ggtcttcgtc tggcgcgtcc aggaatcgaa ctggatctcg ggcggccgat gagcgtaagc ggccacggtt tcaacgatgg tatcgggctt ggccatgtgg tgctgcatga gccgcgcatc gtcgtgacca atcttttcaa tgaggacagt caggccgaac tcaaccggct ggacgaggcg ctcggctcct tgcgtatttc catcgatgac atgttgtcgc gccgcgatgt ggcggtggag ggcgagcatc gcgaggtgct ggaagcctat cgcatgttcg cgcatgatcg cggctgggtg cgtcgtctgg aagaagcggt tcataacggc cttacggcag aagctgccgt ggaaaaggtg cagagcgaca cgcgcgcgcg catggtgcat ctgaccgacc cctatatgcg cgagcgcctt tcggatttcg acgatctcgc caatcgcctc ctgcgccagc ttatgggccg cgacgtcaag acgattgccg aatcactggc caaggatgcg atcatcgttg cccgttccat gggcgcggct gagcttctgg actatccgcg cgaacgcctg cgcggtgtcg tgctggaaga cggggcggcc accagccatg tggtgatcgt ggcgcgcgcc atgggtattc ccgtcgtcgg gcaggccaag ggtgttgttt ccatggcgga aaacaacgat gcgatcatcg tcgatggcga tgagggcatc gtgcatctgc gcccgcaggc cgatgtggaa tccgcctatg cggaaaaggt ccgcttccgt gcacggcggc aggcgcatta ccgtgaattg cgcgacaagc ccgccatcac gaaggatggc gttgatatcg cgcttttgat gaatgccggc ttgcttgtcg atctgccgca gctttccgct tcgggcgctg ccggcattgg cctgtttcgc accgagcttc aattcatggt ggcttccacc ttcccgcgct cggaacagca ggagcggctc taccgttcgg tgatcgatgc cgcaggtgac aagcccgtca cgttccgcac gctggatatt ggtggcgaca aggttctccc ctatttccgc gcaactgcgc atgaggaaaa cccggcgctc ggctggcgcg ccattcgttt gacgctcgac cgtcccggcc tgttgcgcac ccagttgcgc gcgctattga aggcagcggg tggccgtgaa ctgaagctca tgctgcccat ggtgacggaa gtgagcgaag tgaaggccgc gcgcgagatc atcgaacgcg aggttcgcca tctttcccgc tttgcgcatt cgctgccgct gtgtctcaag cttggcgcga tggtggaagt gccggcgcta ttgtggcagc tcgacgaatt gatgacggcg gtggatttcg tttcggtggg ctccaacgac ctgttccagt tcctgatggc ggcggatcgc ggcaattcgc tgatttcgga ccgttttgac cagctttcac catcgttcct gcgtgcgctg cgtcatatcg tgaaagcggg ggcgcggcat gaaaagccgg tgacgctttg cggtgaaatg gcaggccgcc ccttgacggc aatgacgctg atcgggcttg gcttccgttc gatttccatg tcggcggcgg cgatcggccc ggtcaaggcc atgctcggcg cacttgatgc gggcaagctc aatgcgcttc ttctggaaga gctcaacaag ccgaacaatg cgcattcctt gcgcgaattg ttgcttcgat ttgccgaaga caacgaaatt ccgttataaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for BrsuA.18130.a.A1.PW34439
PURIFICATION DATe: 12/2/2011
CONCENTRATION: 7.76mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 50µl
PERCENT IDENTITY: 83
PERCENT COVERAGE: 50
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRELTTGPR VLLKRLRELM AEPLEPQARL DRIVREIAQN MVAEVCSFYI LRADGVLELY ATEGLNPQAV HLAQLRLGQG LVGTIAASAR PLNLTDAQSH PAFAYLPETG EEAYNSFLGV PVLRAGRTLG VLVVQNRTKR AYREDEVEAL ETTAMVLAEI IATGDGLRLA RPGIELDLGR PMSVSGHGFN DGIGLGHVVL HEPRIVVTNL FNEDSQAELN RLDEALGSLR ISIDDMLSRR DVAVEGEHRE VLEAYRMFAH DRGWVRRLEE AVHNGLTAEX AVXKXQSDTR ARMVHLTTPI CASAFXISTI SXSXXXLMGX DXXRXXXXXA IIXPXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXX
Validated NT Sequence
tttgtnnnnt ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgcgtgag ctgacaaccg gtccccgtgt actgctcaag cggttgcgtg aattgatggc ggagccgctg gaaccacagg cgcgccttga caggatcgtc cgcgaaattg cgcagaacat ggtcgcggaa gtctgctcct tctatatatt gcgcgcagac ggcgttcttg aactttacgc caccgaaggt cttaatcccc aagctgtcca ccttgcccag cttcgtctgg ggcagggcct tgtgggcacc attgcggcaa gcgcacgccc gctcaacctg acggatgcgc agagccatcc ggctttcgcc tatctgccgg aaacgggcga ggaagcctat aattccttcc tcggtgtgcc tgtgcttcgc gctggccgca ctttaggcgt tcttgtcgtg cagaatcgca ccaaacgcgc ctatcgagaa gacgaggttg aagcacttga gacaaccgcc atggttctcg ccgaaatcat cgcaaccggg gatggtcttc gtctggcgcg tccaggaatc gaactggatc tcgggcggcc gatgagcgta agcggccacg gtttcaacga tggtatcggg cttggccatg tggtgctgca tgagccgcgc atcgtcgtga ccaatctttt caatgaggac agtcaggccg aactcaaccg gctggacgag gcgctcggct ccttgcgtat ttccatcgat gacatgttgt cgcgccgcga tgtggcggtg gagggcgagc atcgcgaggt gctggaagcc tatcgcatgt tcgcgcatga tcgcggctgg gtgcgtcgtc tggaagaagc ggttcataac ggccttacgg cagaanctgc cgtggnaaag gngcagagcg acacgcgcgc gcgcatggtg catctgacga cccctatatg cgcgagcgcc tttnngattt cgacgatctc nnnntcnncn ncncnnctta tgggnnnnga cntnanncga nngncnannn ntnnngcnat catcnnnccn nnnnggnnnn nnnntgannt nnnnntancc nnnannnnnn nnnnnnnnnn nnnnnnngnn nnnnnnnnnn nnnngnnnnn nnncnngnnn nnnnnnnnnn nnnnng
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRELTTGPR VLLKRLRELM AEPLEPQARL DRIVREIAQN MVAEVCSFYI LRADGVLELY ATEGLNPQAV HLAQLRLGQG LVGTIAASAR PLNLTDAQSH PAFAYLPETG EEAYNSFLGV PVLRAGRTLG VLVVQNRTKR AYREDEVEAL ETTAMVLAEI IATGDGLRLA RPGIELDLGR PMSVSGHGFN DGIGLGHVVL HEPRIVVTNL FNEDSQAELN RLDEALGSLR ISIDDMLSRR DVAVEGEHRE VLEAYRMFAH DRGWVRRLEE AVHNGLTAEA AVEKVQSDTR ARMVHLTDPY MRERLSDFDD LANRLLRQLM GRDVKTIAES LAKDAIIVAR SMGAAELLDY PRERLRGVVL EDGAATSHVV IVARAMGIPV VGQAKGVVSM AENNDAIIVD GDEGIVHLRP QADVESAYAE KVRFRARRQA HYRELRDKPA ITKDGVDIAL LMNAGLLVDL PQLSASGAAG IGLFRTELQF MVASTFPRSE QQERLYRSVI DAAGDKPVTF RTLDIGGDKV LPYFRATAHE ENPALGWRAI RLTLDRPGLL RTQLRALLKA AGGRELKLML PMVTEVSEVK AAREIIEREV RHLSRFAHSL PLCLKLGAMV EVPALLWQLD ELMTAVDFVS VGSNDLFQFL MAADRGNSLI SDRFDQLSPS FLRALRHIVK AGARHEKPVT LCGEMAGRPL TAMTLIGLGF RSISMSAAAI GPVKAMLGAL DAGKLNALLL EELNKPNNAH SLRELLLRFA EDNEIPL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCGTGAGCTG ACAACCGGTC CCCGTGTACT GCTCAAGCGG TTGCGTGAAT TGATGGCGGA GCCGCTGGAA CCACAGGCGC GCCTTGACAG GATCGTCCGC GAAATTGCGC AGAACATGGT CGCGGAAGTC TGCTCCTTCT ATATATTGCG CGCAGACGGC GTTCTTGAAC TTTACGCCAC CGAAGGTCTT AATCCCCAAG CTGTCCACCT TGCCCAGCTT CGTCTGGGGC AGGGCCTTGT GGGCACCATT GCGGCAAGCG CACGCCCGCT CAACCTGACG GATGCGCAGA GCCATCCGGC TTTCGCCTAT CTGCCGGAAA CGGGCGAGGA AGCCTATAAT TCCTTCCTCG GTGTGCCTGT GCTTCGCGCT GGCCGCACTT TAGGCGTTCT TGTCGTGCAG AATCGCACCA AACGCGCCTA TCGAGAAGAC GAGGTTGAAG CACTTGAGAC AACCGCCATG GTTCTCGCCG AAATCATCGC AACCGGGGAT GGTCTTCGTC TGGCGCGTCC AGGAATCGAA CTGGATCTCG GGCGGCCGAT GAGCGTAAGC GGCCACGGTT TCAACGATGG TATCGGGCTT GGCCATGTGG TGCTGCATGA GCCGCGCATC GTCGTGACCA ATCTTTTCAA TGAGGACAGT CAGGCCGAAC TCAACCGGCT GGACGAGGCG CTCGGCTCCT TGCGTATTTC CATCGATGAC ATGTTGTCGC GCCGCGATGT GGCGGTGGAG GGCGAGCATC GCGAGGTGCT GGAAGCCTAT CGCATGTTCG CGCATGATCG CGGCTGGGTG CGTCGTCTGG AAGAAGCGGT TCATAACGGC CTTACGGCAG AAGCTGCCGT GGAAAAGGTG CAGAGCGACA CGCGCGCGCG CATGGTGCAT CTGACCGACC CCTATATGCG CGAGCGCCTT TCGGATTTCG ACGATCTCGC CAATCGCCTC CTGCGCCAGC TTATGGGCCG CGACGTCAAG ACGATTGCCG AATCACTGGC CAAGGATGCG ATCATCGTTG CCCGTTCCAT GGGCGCGGCT GAGCTTCTGG ACTATCCGCG CGAACGCCTG CGCGGTGTCG TGCTGGAAGA CGGGGCGGCC ACCAGCCATG TGGTGATCGT GGCGCGCGCC ATGGGTATTC CCGTCGTCGG GCAGGCCAAG GGTGTTGTTT CCATGGCGGA AAACAACGAT GCGATCATCG TCGATGGCGA TGAGGGCATC GTGCATCTGC GCCCGCAGGC CGATGTGGAA TCCGCCTATG CGGAAAAGGT CCGCTTCCGT GCACGGCGGC AGGCGCATTA CCGTGAATTG CGCGACAAGC CCGCCATCAC GAAGGATGGC GTTGATATCG CGCTTTTGAT GAATGCCGGC TTGCTTGTCG ATCTGCCGCA GCTTTCCGCT TCGGGCGCTG CCGGCATTGG CCTGTTTCGC ACCGAGCTTC AATTCATGGT GGCTTCCACC TTCCCGCGCT CGGAACAGCA GGAGCGGCTC TACCGTTCGG TGATCGATGC CGCAGGTGAC AAGCCCGTCA CGTTCCGCAC GCTGGATATT GGTGGCGACA AGGTTCTCCC CTATTTCCGC GCAACTGCGC ATGAGGAAAA CCCGGCGCTC GGCTGGCGCG CCATTCGTTT GACGCTCGAC CGTCCCGGCC TGTTGCGCAC CCAGTTGCGC GCGCTATTGA AGGCAGCGGG TGGCCGTGAA CTGAAGCTCA TGCTGCCCAT GGTGACGGAA GTGAGCGAAG TGAAGGCCGC GCGCGAGATC ATCGAACGCG AGGTTCGCCA TCTTTCCCGC TTTGCGCATT CGCTGCCGCT GTGTCTCAAG CTTGGCGCGA TGGTGGAAGT GCCGGCGCTA TTGTGGCAGC TCGACGAATT GATGACGGCG GTGGATTTCG TTTCGGTGGG CTCCAACGAC CTGTTCCAGT TCCTGATGGC GGCGGATCGC GGCAATTCGC TGATTTCGGA CCGTTTTGAC CAGCTTTCAC CATCGTTCCT GCGTGCGCTG CGTCATATCG TGAAAGCGGG GGCGCGGCAT GAAAAGCCGG TGACGCTTTG CGGTGAAATG GCAGGCCGCC CCTTGACGGC AATGACGCTG ATCGGGCTTG GCTTCCGTTC GATTTCCATG TCGGCGGCGG CGATCGGCCC GGTCAAGGCC ATGCTCGGCG CACTTGATGC GGGCAAGCTC AATGCGCTTC TTCTGGAAGA GCTCAACAAG CCGAACAATG CGCATTCCTT GCGCGAATTG TTGCTTCGAT TTGCCGAAGA CAACGAAATT CCGTTATAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA