BrsuA.18124.a

Sulfite reductase (NADPH) hemoprotein beta-component (EC 1.8.1.2)

CENTER ID: BrsuA.18124.a
ORGANISM: Brucella suis 1330
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuA.18124.a.A1.GE33946 full length 1 556
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuA.18124.a.A1.PS01427 full length 1 556

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|204722.5.peg.190
RefSeq: NP_697219.1
UniProt: A0A0H3G1J6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MYRYDEFDRD FVAARVAQFK DQVARRLSGE LTEDQFKPLR LMNGLYLQLH AYMLRVAIPY GTLSSRQLRK LGSIARTYDR GFGHFTTRQN IQYNWPALKD VPQILAELAE VEMHSIQTSG NCIRNVTADH FAGAAADEVT DPRPLAEILR QWSSLHPEFS YLPRKFKIAI VGSEHDRAAI QVHDIGLQLK KNEAGELGLA VYVGGGQGRT PMVAKKIRDF LPIEDMLTYV TAIVRVYNLH GRRDNKFKAR IKILVHETGI EQLTREIDAE WEEIRHGELK LPQRDIDVIE SYFRMPDLPQ RPEGWEILAV AQKADAEFAS WVARNVTAHK HPDYAVVTIS LKPVGGIPGD ASAEQMELVA DVAQTCSFDE IRVSHEQNLV LPHVAKADLP AVYDRLQSGG LVTPNAGLIT DIIACPGLDY CALANARSIP VAQRISERFA DLDRQLEIGD LKIKISGCIN ACGHHHVGHI GILGVEKKGE ELYQITLGGS ADENSAIGEI TGRGFSSEEV VDAIETIVDT YLGLRESAEE TFLAAYRRVG MEPFKRALYG EIRSAA
NT Sequence
atgtatcgtt atgatgaatt cgaccgggat tttgttgcag cccgcgttgc ccagttcaag gatcaggttg cgcgccgcct ttcgggcgag ctgacggaag accagttcaa gcccttgcgg ctgatgaacg gcctttacct gcaattgcat gcctatatgc tgcgcgtggc aatcccctat ggaacgctgt ccagccgcca gttgcgcaag cttgggtcta tcgcgcgcac ttatgaccgt ggctttggcc atttcacgac gcgccagaac atccagtata actggcctgc gctgaaggat gttccgcaga ttttggcaga gctggcagag gtggaaatgc attccatcca gacctccggc aattgcattc gcaatgtgac tgccgaccat tttgcaggtg ctgccgccga cgaagtgacg gacccacgcc cgcttgccga aatcctgcgg caatggtcgt cgctgcaccc ggaattttcc tatctgccgc gcaagttcaa gattgcgatt gtcgggtccg agcatgaccg tgcggccatt caggtgcacg atatcggcct gcaactgaag aagaacgagg cgggcgagct tggccttgcc gtttatgtcg gcggcgggca gggccgcacg ccgatggtgg ccaagaaaat tcgtgacttc ctgcccatcg aggacatgct gacctatgtg acggcaatcg tccgtgtcta taatcttcat ggtcggcgtg acaacaagtt caaggcgcgc atcaagatcc ttgtgcatga aaccggcatc gaacaattga cccgcgagat cgatgcggaa tgggaagaaa tccgccatgg cgaattgaag ctgccgcagc gcgatatcga tgtcattgaa agctatttcc gtatgcccga cctgccgcag cgccccgaag gctgggaaat tctcgccgtg gcacagaagg ctgatgcgga atttgcaagc tgggtcgcgc gcaatgtcac cgcgcacaag catcccgatt atgccgtcgt caccatctcg ctgaagcctg tcggcggcat tccgggcgat gccagtgctg aacagatgga actggtggcc gatgtggcgc aaacctgttc cttcgatgaa atccgggtga gccacgagca gaacctcgtg ctgccacatg tggccaaggc cgatctgccg gcagtctacg accgcctgca atcgggcgga ctggtgacac ccaatgccgg tctcatcacc gatatcattg cttgccccgg tcttgattat tgcgcgctgg ccaatgcacg ctccattccc gttgcgcagc gcatttccga gcgcttcgcc gatctggacc gtcagcttga aatcggcgat ctcaagatca agatttccgg ctgcatcaat gcctgcggcc atcatcatgt cggccatatc ggcattctgg gcgtggagaa gaagggtgag gagctttacc agatcacgct tggcggctcg gcggacgaaa actcggccat tggtgaaatc accgggcgcg gtttctcgtc ggaagaggtg gtcgatgcca tcgaaaccat tgtcgatacc tatctgggcc tgcgcgaaag tgcggaggaa actttccttg ccgcttatcg ccgggtggga atggagcctt tcaaacgcgc tctttatggg gagataagaa gtgctgcctg a
Details for BrsuA.18124.a.A1.GE33946
HARVESTED ON: 11/21/2011
SEQUENCED ON: 11/29/2011
EXPECTED MW: 64kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 95
PERCENT COVERAGE: 48
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMYRYDEFDR DFVAARVAQF KDQVARRLSG ELTEDQFKPL RLMNGLYLQL HAYMLRVAIP YGTLSSRQLR KLGSIARTYD RGFGHFTTRQ NIQYNWPALK DVPQILAELA EVEMHSIQTS GNCIRNVTAD HFAGAAADEV TDPRPLAEIL RQWSSLHPEF SYLPRKFKIA IVGSEHDRAA IQVHDIGLQL KKNEAGELGL AXXVGGGQGR TPMVAKKIRD FLPIEDMLTY VTAXVRVYXL HGRRDNKFKA RIXSLXMXXX RX
Validated NT Sequence
antttnntta ctttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtatc gttatgatga attcgaccgg gattttgttg cagcccgcgt tgcccagttc aaggatcagg ttgcgcgccg cctttcgggc gagctgacgg aagaccagtt caagcccttg cggctgatga acggccttta cctgcaattg catgcctata tgctgcgcgt ggcaatcccc tatggaacgc tgtccagccg ccagttgcgc aagcttgggt ctatcgcgcg cacttatgac cgtggctttg gccatttcac gacgcgccag aacatccagt ataactggcc tgcgctgaag gatgttccgc agattttggc agagctggca gaggtggaaa tgcattccat ccagacctcc ggcaattgca ttcgcaatgt gactgccgac cattttgcag gtgctgccgc cgacgaagtg acggacccac gcccgcttgc cgaaatcctg cggcaatggt cgtcgctgca cccggaattt tcctatctgc cgcgcaagtt caagattgcg attgtcgggt ccgagcatga ccgtgcggcc attcaggtgc acgatatcgg cctgcaactg aagaagaacg aggcgggcga gcttggnctt gccgntnntg tcggcggcgg gcagggccgc acgccgatgg tggccaagaa aattcgtgac ttcctgccca tcgaggacat gctgacctat gtgacggcan tcgtccgtgt ctatannctt catggtcggc gtgacaacaa gttcaaggcg cgcatcnnat ccttgngcat gannnggcnt cgacantgac ccgcgnnntc gannnnnang nanaannnnc ntgnnnatgn nncnncnnan cnnnnnnnna nnncntnnan ncnatttncn nnngncnnac tgccnnnnng nnnntnnnnn cnnggnnnnn nnnnnnnngn nnnnntnnna nctggnnnnn nnangnnnnn nnnnnnnnnn nccnnnnnat nnnnnnnntn annnnnnnnn nnnngnnnnn nnnnnnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMYRYDEFDR DFVAARVAQF KDQVARRLSG ELTEDQFKPL RLMNGLYLQL HAYMLRVAIP YGTLSSRQLR KLGSIARTYD RGFGHFTTRQ NIQYNWPALK DVPQILAELA EVEMHSIQTS GNCIRNVTAD HFAGAAADEV TDPRPLAEIL RQWSSLHPEF SYLPRKFKIA IVGSEHDRAA IQVHDIGLQL KKNEAGELGL AVYVGGGQGR TPMVAKKIRD FLPIEDMLTY VTAIVRVYNL HGRRDNKFKA RIKILVHETG IEQLTREIDA EWEEIRHGEL KLPQRDIDVI ESYFRMPDLP QRPEGWEILA VAQKADAEFA SWVARNVTAH KHPDYAVVTI SLKPVGGIPG DASAEQMELV ADVAQTCSFD EIRVSHEQNL VLPHVAKADL PAVYDRLQSG GLVTPNAGLI TDIIACPGLD YCALANARSI PVAQRISERF ADLDRQLEIG DLKIKISGCI NACGHHHVGH IGILGVEKKG EELYQITLGG SADENSAIGE ITGRGFSSEE VVDAIETIVD TYLGLRESAE ETFLAAYRRV GMEPFKRALY GEIRSAA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtatcgttat gatgaattcg accgggattt tgttgcagcc cgcgttgccc agttcaagga tcaggttgcg cgccgccttt cgggcgagct gacggaagac cagttcaagc ccttgcggct gatgaacggc ctttacctgc aattgcatgc ctatatgctg cgcgtggcaa tcccctatgg aacgctgtcc agccgccagt tgcgcaagct tgggtctatc gcgcgcactt atgaccgtgg ctttggccat ttcacgacgc gccagaacat ccagtataac tggcctgcgc tgaaggatgt tccgcagatt ttggcagagc tggcagaggt ggaaatgcat tccatccaga cctccggcaa ttgcattcgc aatgtgactg ccgaccattt tgcaggtgct gccgccgacg aagtgacgga cccacgcccg cttgccgaaa tcctgcggca atggtcgtcg ctgcacccgg aattttccta tctgccgcgc aagttcaaga ttgcgattgt cgggtccgag catgaccgtg cggccattca ggtgcacgat atcggcctgc aactgaagaa gaacgaggcg ggcgagcttg gccttgccgt ttatgtcggc ggcgggcagg gccgcacgcc gatggtggcc aagaaaattc gtgacttcct gcccatcgag gacatgctga cctatgtgac ggcaatcgtc cgtgtctata atcttcatgg tcggcgtgac aacaagttca aggcgcgcat caagatcctt gtgcatgaaa ccggcatcga acaattgacc cgcgagatcg atgcggaatg ggaagaaatc cgccatggcg aattgaagct gccgcagcgc gatatcgatg tcattgaaag ctatttccgt atgcccgacc tgccgcagcg ccccgaaggc tgggaaattc tcgccgtggc acagaaggct gatgcggaat ttgcaagctg ggtcgcgcgc aatgtcaccg cgcacaagca tcccgattat gccgtcgtca ccatctcgct gaagcctgtc ggcggcattc cgggcgatgc cagtgctgaa cagatggaac tggtggccga tgtggcgcaa acctgttcct tcgatgaaat ccgggtgagc cacgagcaga acctcgtgct gccacatgtg gccaaggccg atctgccggc agtctacgac cgcctgcaat cgggcggact ggtgacaccc aatgccggtc tcatcaccga tatcattgct tgccccggtc ttgattattg cgcgctggcc aatgcacgct ccattcccgt tgcgcagcgc atttccgagc gcttcgccga tctggaccgt cagcttgaaa tcggcgatct caagatcaag atttccggct gcatcaatgc ctgcggccat catcatgtcg gccatatcgg cattctgggc gtggagaaga agggtgagga gctttaccag atcacgcttg gcggctcggc ggacgaaaac tcggccattg gtgaaatcac cgggcgcggt ttctcgtcgg aagaggtggt cgatgccatc gaaaccattg tcgataccta tctgggcctg cgcgaaagtg cggaggaaac tttccttgcc gcttatcgcc gggtgggaat ggagcctttc aaacgcgctc tttatgggga gataagaagt gctgcctaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for BrsuA.18124.a.A1.PS01427
PURIFICATION DATe: 3/15/2012
CONCENTRATION: 10.5mg/ml
OBSERVED MW: 64kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: 95
PERCENT COVERAGE: 48
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMYRYDEFDR DFVAARVAQF KDQVARRLSG ELTEDQFKPL RLMNGLYLQL HAYMLRVAIP YGTLSSRQLR KLGSIARTYD RGFGHFTTRQ NIQYNWPALK DVPQILAELA EVEMHSIQTS GNCIRNVTAD HFAGAAADEV TDPRPLAEIL RQWSSLHPEF SYLPRKFKIA IVGSEHDRAA IQVHDIGLQL KKNEAGELGL AXXVGGGQGR TPMVAKKIRD FLPIEDMLTY VTAXVRVYXL HGRRDNKFKA RIXSLXMXXX RX
Validated NT Sequence
antttnntta ctttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtatc gttatgatga attcgaccgg gattttgttg cagcccgcgt tgcccagttc aaggatcagg ttgcgcgccg cctttcgggc gagctgacgg aagaccagtt caagcccttg cggctgatga acggccttta cctgcaattg catgcctata tgctgcgcgt ggcaatcccc tatggaacgc tgtccagccg ccagttgcgc aagcttgggt ctatcgcgcg cacttatgac cgtggctttg gccatttcac gacgcgccag aacatccagt ataactggcc tgcgctgaag gatgttccgc agattttggc agagctggca gaggtggaaa tgcattccat ccagacctcc ggcaattgca ttcgcaatgt gactgccgac cattttgcag gtgctgccgc cgacgaagtg acggacccac gcccgcttgc cgaaatcctg cggcaatggt cgtcgctgca cccggaattt tcctatctgc cgcgcaagtt caagattgcg attgtcgggt ccgagcatga ccgtgcggcc attcaggtgc acgatatcgg cctgcaactg aagaagaacg aggcgggcga gcttggnctt gccgntnntg tcggcggcgg gcagggccgc acgccgatgg tggccaagaa aattcgtgac ttcctgccca tcgaggacat gctgacctat gtgacggcan tcgtccgtgt ctatannctt catggtcggc gtgacaacaa gttcaaggcg cgcatcnnat ccttgngcat gannnggcnt cgacantgac ccgcgnnntc gannnnnang nanaannnnc ntgnnnatgn nncnncnnan cnnnnnnnna nnncntnnan ncnatttncn nnngncnnac tgccnnnnng nnnntnnnnn cnnggnnnnn nnnnnnnngn nnnnntnnna nctggnnnnn nnangnnnnn nnnnnnnnnn nccnnnnnat nnnnnnnntn annnnnnnnn nnnngnnnnn nnnnnnna
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMYRYDEFDR DFVAARVAQF KDQVARRLSG ELTEDQFKPL RLMNGLYLQL HAYMLRVAIP YGTLSSRQLR KLGSIARTYD RGFGHFTTRQ NIQYNWPALK DVPQILAELA EVEMHSIQTS GNCIRNVTAD HFAGAAADEV TDPRPLAEIL RQWSSLHPEF SYLPRKFKIA IVGSEHDRAA IQVHDIGLQL KKNEAGELGL AVYVGGGQGR TPMVAKKIRD FLPIEDMLTY VTAIVRVYNL HGRRDNKFKA RIKILVHETG IEQLTREIDA EWEEIRHGEL KLPQRDIDVI ESYFRMPDLP QRPEGWEILA VAQKADAEFA SWVARNVTAH KHPDYAVVTI SLKPVGGIPG DASAEQMELV ADVAQTCSFD EIRVSHEQNL VLPHVAKADL PAVYDRLQSG GLVTPNAGLI TDIIACPGLD YCALANARSI PVAQRISERF ADLDRQLEIG DLKIKISGCI NACGHHHVGH IGILGVEKKG EELYQITLGG SADENSAIGE ITGRGFSSEE VVDAIETIVD TYLGLRESAE ETFLAAYRRV GMEPFKRALY GEIRSAA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTATCGTTAT GATGAATTCG ACCGGGATTT TGTTGCAGCC CGCGTTGCCC AGTTCAAGGA TCAGGTTGCG CGCCGCCTTT CGGGCGAGCT GACGGAAGAC CAGTTCAAGC CCTTGCGGCT GATGAACGGC CTTTACCTGC AATTGCATGC CTATATGCTG CGCGTGGCAA TCCCCTATGG AACGCTGTCC AGCCGCCAGT TGCGCAAGCT TGGGTCTATC GCGCGCACTT ATGACCGTGG CTTTGGCCAT TTCACGACGC GCCAGAACAT CCAGTATAAC TGGCCTGCGC TGAAGGATGT TCCGCAGATT TTGGCAGAGC TGGCAGAGGT GGAAATGCAT TCCATCCAGA CCTCCGGCAA TTGCATTCGC AATGTGACTG CCGACCATTT TGCAGGTGCT GCCGCCGACG AAGTGACGGA CCCACGCCCG CTTGCCGAAA TCCTGCGGCA ATGGTCGTCG CTGCACCCGG AATTTTCCTA TCTGCCGCGC AAGTTCAAGA TTGCGATTGT CGGGTCCGAG CATGACCGTG CGGCCATTCA GGTGCACGAT ATCGGCCTGC AACTGAAGAA GAACGAGGCG GGCGAGCTTG GCCTTGCCGT TTATGTCGGC GGCGGGCAGG GCCGCACGCC GATGGTGGCC AAGAAAATTC GTGACTTCCT GCCCATCGAG GACATGCTGA CCTATGTGAC GGCAATCGTC CGTGTCTATA ATCTTCATGG TCGGCGTGAC AACAAGTTCA AGGCGCGCAT CAAGATCCTT GTGCATGAAA CCGGCATCGA ACAATTGACC CGCGAGATCG ATGCGGAATG GGAAGAAATC CGCCATGGCG AATTGAAGCT GCCGCAGCGC GATATCGATG TCATTGAAAG CTATTTCCGT ATGCCCGACC TGCCGCAGCG CCCCGAAGGC TGGGAAATTC TCGCCGTGGC ACAGAAGGCT GATGCGGAAT TTGCAAGCTG GGTCGCGCGC AATGTCACCG CGCACAAGCA TCCCGATTAT GCCGTCGTCA CCATCTCGCT GAAGCCTGTC GGCGGCATTC CGGGCGATGC CAGTGCTGAA CAGATGGAAC TGGTGGCCGA TGTGGCGCAA ACCTGTTCCT TCGATGAAAT CCGGGTGAGC CACGAGCAGA ACCTCGTGCT GCCACATGTG GCCAAGGCCG ATCTGCCGGC AGTCTACGAC CGCCTGCAAT CGGGCGGACT GGTGACACCC AATGCCGGTC TCATCACCGA TATCATTGCT TGCCCCGGTC TTGATTATTG CGCGCTGGCC AATGCACGCT CCATTCCCGT TGCGCAGCGC ATTTCCGAGC GCTTCGCCGA TCTGGACCGT CAGCTTGAAA TCGGCGATCT CAAGATCAAG ATTTCCGGCT GCATCAATGC CTGCGGCCAT CATCATGTCG GCCATATCGG CATTCTGGGC GTGGAGAAGA AGGGTGAGGA GCTTTACCAG ATCACGCTTG GCGGCTCGGC GGACGAAAAC TCGGCCATTG GTGAAATCAC CGGGCGCGGT TTCTCGTCGG AAGAGGTGGT CGATGCCATC GAAACCATTG TCGATACCTA TCTGGGCCTG CGCGAAAGTG CGGAGGAAAC TTTCCTTGCC GCTTATCGCC GGGTGGGAAT GGAGCCTTTC AAACGCGCTC TTTATGGGGA GATAAGAAGT GCTGCCTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA