SSGCID: Target Page

BrsuA.18109.a

SIS domain protein

CENTER ID:	BrsuA.18109.a
ORGANISM:	Brucella suis 1330
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuA.18109.a.A1.GE33974	full length		1	256

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|204722.5.peg.2927
RefSeq:	NP_699892.1
UniProt:	A0A0H3GFJ5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLEVADRLPK RLKQCADYVA ANQDRIAVST VAEMAEGAGV QSSAFMRFCQ ILGFSGFSEM QRLFRETYAG GWPDYSTRLL HLRETAAGRA PALLAEFAEA GRTSLEGLLK TIEPEALEKA VELLASANTI HIIGIRRAFP VASYLAYALE KMAVPAMLHS AVGKLENLHA IRKGDVLLAI TFSPYSPETI GMTEAVAARG IDVIGITDTI VSPMSKVARQ TLLVSEVDFG AFRSLSATLC LAIAIAVAVG TARQAG

NT Sequence

ttgctggagg ttgccgaccg cctgcccaag cgcctgaagc aatgcgccga ttatgtcgcc gccaatcagg atcgcatcgc ggtttccacc gtggcggaaa tggccgaagg tgcgggcgtg caaagttctg ctttcatgcg cttttgccag attttgggtt tttcgggttt ttcggaaatg cagcgccttt ttcgcgaaac ctatgcgggc ggctggccgg attattccac ccgcctcctg catttgcgtg aaacagcggc aggtagagcg ccagcacttc tggccgaatt tgccgaggcc gggcgcacct cgcttgaggg acttctgaaa acgatcgaac cggaggcgct ggaaaaggcc gtggagctgc tcgccagcgc caacacgatc catatcatcg gtatccgccg cgcctttccg gttgcaagct atctcgccta tgcgctggaa aaaatggcgg ttccggccat gctgcacagt gcagtcggca aactggagaa cctccacgca atccgcaagg gcgatgtgct tctggccatt accttctcgc cttactcgcc ggaaacgatc ggcatgaccg aggctgttgc cgcgcggggc attgatgtca tcggcattac cgacacgatc gtaagcccca tgagcaaggt cgccagacag acacttctcg tttccgaagt ggattttggt gcgtttcgct cgctttcggc aacactttgt cttgccatcg ccattgctgt tgcggtgggc actgcccgtc aggctggata g

Details for BrsuA.18109.a.A1.GE33974

HARVESTED ON:	11/21/2011
SEQUENCED ON:	11/29/2011
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	96
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLLEVADRL PKRLKQCADY VAANQDRIAV STVAEMAEGA GVQSSAFMRF CQILGFSGFS EMQRLFRETY AGGWPDYSTR LLHLRETAAG RAPALLAEFA EAGRTSLEGL LKTIEPEALE KAVELLASAN TIHIIGIRRA FPVASYLAYA LEKMAVPAML HSAVGKLENL HAIRKGDVLL AITFSPYSPE TIGMTEAXAA RGIDVIGITD TIVSPMSKXA RQTLLXSXVX FXAFRSLSAT LCLAXAIAVA VGTXRQXGX

Validated NT Sequence

attttnntta ctttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttgc tggaggttgc cgaccgcctg cccaagcgcc tgaagcaatg cgccgattat gtcgccgcca atcaggatcg catcgcggtt tccaccgtgg cggaaatggc cgaaggtgcg ggcgtgcaaa gttctgcttt catgcgcttt tgccagattt tgggtttttc gggtttttcg gaaatgcagc gcctttttcg cgaaacctat gcgggcggct ggccggatta ttccacccgc ctcctgcatt tgcgtgaaac agcggcaggt agagcgccag cacttctggc cgaatttgcc gaggccgggc gcacctcgct tgagggactt ctgaaaacga tcgaaccgga ggcgctggaa aaggccgtgg agctgctcgc cagcgccaac acgatccata tcatcggtat ccgccgcgcc tttccggttg caagctatct cgcctatgcg ctggaaaaaa tggcggttcc ggccatgctg cacagtgcag tcggcaaact ggagaacctc cacgcaatcc gcaagggcga tgtgcttctg gccattacct tctcgcctta ctcgccggaa acgatcggca tgaccgaggc tgntgccgcg cggggcattg atgtcatcgg cattaccgac acgatcgtaa gccccatgag caaggncgcc agacagacac ttctcgnttc cnaagtggan tttgntgcgt ttcgctcgct ttcggcaaca ctttgtcttg ccntcgccat tgctgttgcg gtgggcactg nccgtcaggn tgganaataa acagcacgaa caanntnntg nagccaagct tcnncgagga tccggctgct aacaaagccc aaaggaagnt nanttnntnn nnnnnnnnnn nnaaaaaaaa aaaaaaaaaa nnnnncnngt ttgnngccan gnaatcnttc cnnnnnanag nggtcanccc tnnnnncnnc ccngcnnnnn nnnngncnnn nnncnnnnnc nttnntngan naanannnnn nntaannnnn nncc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMLLEVADRL PKRLKQCADY VAANQDRIAV STVAEMAEGA GVQSSAFMRF CQILGFSGFS EMQRLFRETY AGGWPDYSTR LLHLRETAAG RAPALLAEFA EAGRTSLEGL LKTIEPEALE KAVELLASAN TIHIIGIRRA FPVASYLAYA LEKMAVPAML HSAVGKLENL HAIRKGDVLL AITFSPYSPE TIGMTEAVAA RGIDVIGITD TIVSPMSKVA RQTLLVSEVD FGAFRSLSAT LCLAIAIAVA VGTARQAG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgctggag gttgccgacc gcctgcccaa gcgcctgaag caatgcgccg attatgtcgc cgccaatcag gatcgcatcg cggtttccac cgtggcggaa atggccgaag gtgcgggcgt gcaaagttct gctttcatgc gcttttgcca gattttgggt ttttcgggtt tttcggaaat gcagcgcctt tttcgcgaaa cctatgcggg cggctggccg gattattcca cccgcctcct gcatttgcgt gaaacagcgg caggtagagc gccagcactt ctggccgaat ttgccgaggc cgggcgcacc tcgcttgagg gacttctgaa aacgatcgaa ccggaggcgc tggaaaaggc cgtggagctg ctcgccagcg ccaacacgat ccatatcatc ggtatccgcc gcgcctttcc ggttgcaagc tatctcgcct atgcgctgga aaaaatggcg gttccggcca tgctgcacag tgcagtcggc aaactggaga acctccacgc aatccgcaag ggcgatgtgc ttctggccat taccttctcg ccttactcgc cggaaacgat cggcatgacc gaggctgttg ccgcgcgggg cattgatgtc atcggcatta ccgacacgat cgtaagcccc atgagcaagg tcgccagaca gacacttctc gtttccgaag tggattttgg tgcgtttcgc tcgctttcgg caacactttg tcttgccatc gccattgctg ttgcggtggg cactgcccgt caggctggat aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa