BrsuA.18107.a

Renal dipeptidase family protein

CENTER ID: BrsuA.18107.a
ORGANISM: Brucella suis 1330
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuA.18107.a.A1.GE33955 full length 1 346
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuA.18107.a.A1.PW34626 full length 1 346

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|204722.5.peg.2867
RefSeq: NP_699838.1
UniProt: A0A0H3G6Q7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MATEQTIKVF DGHNDVLLRL WSSNQPEPEN RFLEDESSGH IDLPRARKGG LGGGLCAVYV PSPSHDLDAD GNLPTPSQQD ALKTTLAMSA ILQNIERKSE GQVRICRTAA DIRDAFAKDI FASVYHIEGV EAFDADLDAL YVLHAAGLRS LGPVWSRPNI FAHGIPFRFP SSPDIGPGLT DHGKALIRAC NELKVMIDLS HMNEKGFWDI AALSDAPLVA SHSNAHVLCQ QSRNLTDRQL DAIRDTGGLV GLNFGVSFLR EDGKHDPDTK LEELVRHADY IVGRIGIDHV AFGSDFDGTI ISSTLRDVAD LQLVIETFRK HGYDDASLVK LAHGNWIDVL ERTWGK
NT Sequence
atggcgacag aacagacgat caaggttttc gacggtcata atgatgtatt gctgcgcctg tggtcgtcaa accagcccga acccgaaaat cgcttccttg aagacgaaag cagcgggcat atcgacttgc cccgtgcacg taagggcgga ctgggtggcg gtctctgtgc ggtctacgtc ccctcacctt cgcacgatct tgatgcggac ggcaacctgc cgacgccaag ccagcaggat gccctgaaga caacgctcgc catgtcggcc atcctccaga acatcgagcg caaatccgaa ggccaggttc gcatttgccg cactgcggcc gatattcgcg atgccttcgc caaagacatc ttcgcctcgg tctatcatat cgagggcgtg gaagccttcg atgccgatct cgacgcgctt tatgtgcttc atgcggcggg cctgaggtca cttgggcccg tctggagccg cccgaatatc tttgcccatg gcattccgtt ccgctttccg tcatcgcccg atatcggacc gggcctcacc gaccatggca aggcccttat ccgcgcctgc aatgaattga aggtgatgat cgacctttcg catatgaacg aaaaaggctt ctgggacatt gccgccttgt ccgatgcacc gctggttgcc tcacactcca atgcccatgt gctttgccag caaagccgca acctcaccga caggcagctt gacgccatcc gcgacaccgg cggtctggtg gggctcaact tcggcgtttc attcctgcgc gaagacggca agcacgatcc tgatacgaag cttgaggaac tggtgcgcca tgcggattat atcgtgggcc gcatcggcat cgatcatgtg gcattcggct cggatttcga cggcaccatc atatcctcca ccttgcgcga tgtggccgat ctacagcttg tgatcgaaac cttccgcaag catggctatg acgatgcctc cctcgtcaag ctggcgcatg gaaactggat tgacgtgctg gaacgcacct gggggaagtg a
Details for BrsuA.18107.a.A1.GE33955
HARVESTED ON: 11/21/2011
SEQUENCED ON: 11/29/2011
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Validated AA Sequence
MATEQTIKVF DGHNDVLLRL WSSNQPEPEN RFLEDESSGH IDLPRARKGG LGGGLCAVYV PSPSHDLDAD GNLPTPSQQD ALKTTLAMSA ILQNIERKSE GQVRICRTAA DIRDAFAKDI FASVYHIEGV EAFDADLDAL YVLHAAGLRS LGPVWSRPNI FAHGIPFRFP SSPDIGPGLT DHGKALIRAC NELKVMIDLS HMNEKGFWDI AALSDAPLVA SHSNAHVLCQ QSRNLTDRQL DAIRDTGGLV GLNFGVSFLR EDGKHDPDTK LEELVRHADY IVGRIGIDHV AFGSDFDGTI ISSTLRDVAD LQLVIETFRK HGYDDASLVK LAHGNWIDVL ERTW
Validated NT Sequence
cccccaggtg cgttccagca cgtcaatcca gtttccatgc gccagcttga cgagggaggc atcgtcatag ccatgcttgc ggaaggtttc gatcacaagc tgtagatcgg ccacatcgcg caaggtggag gatatgatgg tgccgtcgaa atccgagccg aatgccacat gatcgatgcc gatgcggccc acgatataat ccgcatggcg caccagttcc tcaagcttcg tatcaggatc gtgcttgccg tcttcgcgca ggaatgaaac gccgaagttg agccccacca gaccgccggt gtcgcggatg gcgtcaagct gcctgtcggt gaggttgcgg ctttgctggc aaagcacatg ggcattggag tgtgaggcaa ccagcggtgc atcggacaag gcggcaatgt cccagaagcc tttttcgttc atatgcgaaa ggtcgatcat caccttcaat tcattgcagg cgcggataag ggccttgcca tggtcggtga ggcccggtcc gatatcgggc gatgacggaa agcggaacgg aatgccatgg gcaaagatat tcgggcggct ccagacgggc ccaagtgacc tcaggcccgc cgcatgaagc acataaagcg cgtcgagatc ggcatcgaag gcttccacgc cctcgatatg atagaccgag gcgaagatgt ctttggcgaa ggcatcgcga atatcggccg cagtgcggca aatgcgaacc tggccttcgg atttgcgctc gatgttctgg aggatggccg acatggcgag cgttgtcttc agggcatcct gctggcttgg cgtcggcagg ttgccgtccg catcaagatc gtgcgaaggt gaggggacgt agaccgcaca gagaccgcca cccagtccgc ccttacgtgc acggggcaag tcgatatgcc cgctgctttc gtcttcaagg aagcgatttt cgggttcggg ctggtttgac gaccacaggc gcagcaatac atcattatga ccgtcgaaaa ccttgatcgt ctgttctgtc gccatcgaac caggaccctg ggtctgagct tccagggtac
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMATEQTIKV FDGHNDVLLR LWSSNQPEPE NRFLEDESSG HIDLPRARKG GLGGGLCAVY VPSPSHDLDA DGNLPTPSQQ DALKTTLAMS AILQNIERKS EGQVRICRTA ADIRDAFAKD IFASVYHIEG VEAFDADLDA LYVLHAAGLR SLGPVWSRPN IFAHGIPFRF PSSPDIGPGL TDHGKALIRA CNELKVMIDL SHMNEKGFWD IAALSDAPLV ASHSNAHVLC QQSRNLTDRQ LDAIRDTGGL VGLNFGVSFL REDGKHDPDT KLEELVRHAD YIVGRIGIDH VAFGSDFDGT IISSTLRDVA DLQLVIETFR KHGYDDASLV KLAHGNWIDV LERTWGK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgacagaa cagacgatca aggttttcga cggtcataat gatgtattgc tgcgcctgtg gtcgtcaaac cagcccgaac ccgaaaatcg cttccttgaa gacgaaagca gcgggcatat cgacttgccc cgtgcacgta agggcggact gggtggcggt ctctgtgcgg tctacgtccc ctcaccttcg cacgatcttg atgcggacgg caacctgccg acgccaagcc agcaggatgc cctgaagaca acgctcgcca tgtcggccat cctccagaac atcgagcgca aatccgaagg ccaggttcgc atttgccgca ctgcggccga tattcgcgat gccttcgcca aagacatctt cgcctcggtc tatcatatcg agggcgtgga agccttcgat gccgatctcg acgcgcttta tgtgcttcat gcggcgggcc tgaggtcact tgggcccgtc tggagccgcc cgaatatctt tgcccatggc attccgttcc gctttccgtc atcgcccgat atcggaccgg gcctcaccga ccatggcaag gcccttatcc gcgcctgcaa tgaattgaag gtgatgatcg acctttcgca tatgaacgaa aaaggcttct gggacattgc cgccttgtcc gatgcaccgc tggttgcctc acactccaat gcccatgtgc tttgccagca aagccgcaac ctcaccgaca ggcagcttga cgccatccgc gacaccggcg gtctggtggg gctcaacttc ggcgtttcat tcctgcgcga agacggcaag cacgatcctg atacgaagct tgaggaactg gtgcgccatg cggattatat cgtgggccgc atcggcatcg atcatgtggc attcggctcg gatttcgacg gcaccatcat atcctccacc ttgcgcgatg tggccgatct acagcttgtg atcgaaacct tccgcaagca tggctatgac gatgcctccc tcgtcaagct ggcgcatgga aactggattg acgtgctgga acgcacctgg gggaagtaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for BrsuA.18107.a.A1.PW34626
PURIFICATION DATe: 1/24/2012
CONCENTRATION: 26.94mg/ml
OBSERVED MW: 38kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Protocol Notes
notes unavailable
Validated AA Sequence
MATEQTIKVF DGHNDVLLRL WSSNQPEPEN RFLEDESSGH IDLPRARKGG LGGGLCAVYV PSPSHDLDAD GNLPTPSQQD ALKTTLAMSA ILQNIERKSE GQVRICRTAA DIRDAFAKDI FASVYHIEGV EAFDADLDAL YVLHAAGLRS LGPVWSRPNI FAHGIPFRFP SSPDIGPGLT DHGKALIRAC NELKVMIDLS HMNEKGFWDI AALSDAPLVA SHSNAHVLCQ QSRNLTDRQL DAIRDTGGLV GLNFGVSFLR EDGKHDPDTK LEELVRHADY IVGRIGIDHV AFGSDFDGTI ISSTLRDVAD LQLVIETFRK HGYDDASLVK LAHGNWIDVL ERTW
Validated NT Sequence
cccccaggtg cgttccagca cgtcaatcca gtttccatgc gccagcttga cgagggaggc atcgtcatag ccatgcttgc ggaaggtttc gatcacaagc tgtagatcgg ccacatcgcg caaggtggag gatatgatgg tgccgtcgaa atccgagccg aatgccacat gatcgatgcc gatgcggccc acgatataat ccgcatggcg caccagttcc tcaagcttcg tatcaggatc gtgcttgccg tcttcgcgca ggaatgaaac gccgaagttg agccccacca gaccgccggt gtcgcggatg gcgtcaagct gcctgtcggt gaggttgcgg ctttgctggc aaagcacatg ggcattggag tgtgaggcaa ccagcggtgc atcggacaag gcggcaatgt cccagaagcc tttttcgttc atatgcgaaa ggtcgatcat caccttcaat tcattgcagg cgcggataag ggccttgcca tggtcggtga ggcccggtcc gatatcgggc gatgacggaa agcggaacgg aatgccatgg gcaaagatat tcgggcggct ccagacgggc ccaagtgacc tcaggcccgc cgcatgaagc acataaagcg cgtcgagatc ggcatcgaag gcttccacgc cctcgatatg atagaccgag gcgaagatgt ctttggcgaa ggcatcgcga atatcggccg cagtgcggca aatgcgaacc tggccttcgg atttgcgctc gatgttctgg aggatggccg acatggcgag cgttgtcttc agggcatcct gctggcttgg cgtcggcagg ttgccgtccg catcaagatc gtgcgaaggt gaggggacgt agaccgcaca gagaccgcca cccagtccgc ccttacgtgc acggggcaag tcgatatgcc cgctgctttc gtcttcaagg aagcgatttt cgggttcggg ctggtttgac gaccacaggc gcagcaatac atcattatga ccgtcgaaaa ccttgatcgt ctgttctgtc gccatcgaac caggaccctg ggtctgagct tccagggtac
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMATEQTIKV FDGHNDVLLR LWSSNQPEPE NRFLEDESSG HIDLPRARKG GLGGGLCAVY VPSPSHDLDA DGNLPTPSQQ DALKTTLAMS AILQNIERKS EGQVRICRTA ADIRDAFAKD IFASVYHIEG VEAFDADLDA LYVLHAAGLR SLGPVWSRPN IFAHGIPFRF PSSPDIGPGL TDHGKALIRA CNELKVMIDL SHMNEKGFWD IAALSDAPLV ASHSNAHVLC QQSRNLTDRQ LDAIRDTGGL VGLNFGVSFL REDGKHDPDT KLEELVRHAD YIVGRIGIDH VAFGSDFDGT IISSTLRDVA DLQLVIETFR KHGYDDASLV KLAHGNWIDV LERTWGK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGACAGAA CAGACGATCA AGGTTTTCGA CGGTCATAAT GATGTATTGC TGCGCCTGTG GTCGTCAAAC CAGCCCGAAC CCGAAAATCG CTTCCTTGAA GACGAAAGCA GCGGGCATAT CGACTTGCCC CGTGCACGTA AGGGCGGACT GGGTGGCGGT CTCTGTGCGG TCTACGTCCC CTCACCTTCG CACGATCTTG ATGCGGACGG CAACCTGCCG ACGCCAAGCC AGCAGGATGC CCTGAAGACA ACGCTCGCCA TGTCGGCCAT CCTCCAGAAC ATCGAGCGCA AATCCGAAGG CCAGGTTCGC ATTTGCCGCA CTGCGGCCGA TATTCGCGAT GCCTTCGCCA AAGACATCTT CGCCTCGGTC TATCATATCG AGGGCGTGGA AGCCTTCGAT GCCGATCTCG ACGCGCTTTA TGTGCTTCAT GCGGCGGGCC TGAGGTCACT TGGGCCCGTC TGGAGCCGCC CGAATATCTT TGCCCATGGC ATTCCGTTCC GCTTTCCGTC ATCGCCCGAT ATCGGACCGG GCCTCACCGA CCATGGCAAG GCCCTTATCC GCGCCTGCAA TGAATTGAAG GTGATGATCG ACCTTTCGCA TATGAACGAA AAAGGCTTCT GGGACATTGC CGCCTTGTCC GATGCACCGC TGGTTGCCTC ACACTCCAAT GCCCATGTGC TTTGCCAGCA AAGCCGCAAC CTCACCGACA GGCAGCTTGA CGCCATCCGC GACACCGGCG GTCTGGTGGG GCTCAACTTC GGCGTTTCAT TCCTGCGCGA AGACGGCAAG CACGATCCTG ATACGAAGCT TGAGGAACTG GTGCGCCATG CGGATTATAT CGTGGGCCGC ATCGGCATCG ATCATGTGGC ATTCGGCTCG GATTTCGACG GCACCATCAT ATCCTCCACC TTGCGCGATG TGGCCGATCT ACAGCTTGTG ATCGAAACCT TCCGCAAGCA TGGCTATGAC GATGCCTCCC TCGTCAAGCT GGCGCATGGA AACTGGATTG ACGTGCTGGA ACGCACCTGG GGGAAGTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA