BrsuA.18082.a

FIG00641944: hypothetical protein

CENTER ID: BrsuA.18082.a
ORGANISM: Brucella suis 1330
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuA.18082.a.B1.GE36816 Full length( BrsuA.18082.a ) 1 424
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuA.18082.a.B1.PS01859 Full length( BrsuA.18082.a ) 1 424
BrsuA.18082.a.B1.PS01860 Full length( BrsuA.18082.a ) 1 424

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruSui107850_2365
BV-BRC: fig|204722.5.peg.2365
RefSeq: NP_699351.1
RefSeq: YP_005613861.1
UniProt: A0A0H3G6C4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRLGVIADDF TGATDIAGFL VGNGLRTIQL NGVPADDLAV DADAVVISLK TRSCPTGQAI AESLAALKWL QKNNCQQFFF KYCSTFDSTP KGNIGPVTDA LLEALGEEFT VICPALPVNG RTIYNGYLFV NGVLLSETGM RNHPVTPMTD SNIMRVMESQ SRGRAGNISS TIVDQGSDAV RDALRKLQSE GIRYAVLDAL NDQHIETLGR AVSQMKLVTG GSGLADGMAR AWTQLRGKNV AAAEAAGAPV KGRTVILSGS CSQMTNAQVA AYKAKAPALA MDVEKAINDA AYIDVLAEWV LAQSGDALAP LVYATMPPEA LKAVQERFGG ERASAAIEDL FGQLAKRLEA EGFTRFIVAG GETSGAVTQA LAIDGFTIRP QIAPGVPWVR GIGKPLSLAL KSGNFGTEAF FFEAQKIANQ EGDK
NT Sequence
ATGCGCCTTG GCGTCATTGC AGACGATTTT ACCGGAGCAA CCGATATCGC AGGATTTCTG GTTGGCAACG GCCTGCGCAC CATTCAGCTG AATGGCGTTC CAGCGGACGA CCTTGCCGTG GATGCGGATG CGGTCGTCAT CAGCCTGAAG ACCCGTTCCT GCCCGACCGG GCAAGCAATT GCCGAAAGCC TCGCGGCGCT GAAATGGCTG CAAAAAAACA ACTGCCAGCA ATTTTTCTTC AAATATTGCT CCACCTTCGA CAGCACGCCA AAAGGCAATA TCGGGCCTGT CACCGATGCA TTGCTTGAGG CACTGGGTGA AGAATTCACC GTCATCTGCC CTGCCCTGCC CGTCAATGGC CGTACCATCT ATAATGGCTA TCTGTTTGTG AACGGCGTTT TGTTGAGCGA AACCGGGATG CGAAACCACC CCGTCACGCC CATGACCGAC AGCAACATCA TGCGGGTAAT GGAATCCCAG TCGCGCGGAC GTGCAGGAAA TATTTCCAGC ACTATCGTCG ATCAGGGCAG CGATGCAGTG CGCGACGCAC TCAGGAAGCT TCAGTCCGAA GGCATCCGCT ATGCCGTCCT CGACGCCCTG AACGACCAGC ATATAGAAAC GCTTGGGCGT GCCGTATCGC AAATGAAGCT GGTTACGGGC GGCTCGGGCC TTGCAGACGG GATGGCGCGC GCATGGACAC AACTGCGCGG CAAGAATGTT GCGGCGGCTG AAGCTGCCGG CGCGCCTGTG AAGGGACGCA CGGTCATCCT CTCCGGTTCC TGCTCCCAGA TGACCAATGC GCAGGTGGCC GCATACAAGG CAAAGGCCCC CGCGCTGGCC ATGGATGTCG AGAAGGCCAT AAACGACGCG GCCTATATCG ATGTTCTGGC CGAATGGGTG CTCGCACAGT CTGGCGATGC TTTGGCGCCG CTCGTTTATG CAACCATGCC GCCGGAAGCA CTGAAAGCAG TGCAGGAGCG ATTTGGTGGC GAACGTGCAA GCGCTGCCAT CGAAGATTTG TTCGGGCAGC TTGCGAAACG TCTCGAAGCC GAAGGGTTTA CGCGCTTCAT TGTTGCAGGC GGAGAAACAT CCGGGGCTGT GACACAGGCT TTGGCAATCG ATGGGTTTAC CATCAGGCCC CAGATTGCGC CCGGCGTTCC CTGGGTACGC GGGATCGGAA AACCATTATC GCTTGCACTG AAATCCGGAA ATTTCGGCAC TGAGGCTTTC TTCTTTGAAG CCCAAAAGAT TGCCAATCAG GAAGGAGATA AA
Details for BrsuA.18082.a.B1.GE36816
HARVESTED ON: 3/27/2013
SEQUENCED ON: 3/30/2013
EXPECTED MW: 46kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 63
Validated AA Sequence
MRNHPVTPMT DSNIMRVMES QSRGRAGNIS STIVDQGSDA VRDALRKLQS EGIRYAVLDA LNDQHIETLG RAVSQMKLVT GGSGLADGMA RAWTQLRGKN VAAAEAAGAP VKGRTVILSG SCSQMTNAQV AAYKAKAPAL AMDVEKAIND AAYIDVLAEW VLAQSGDALA PLVYATMPPE ALKAVQERFG GERASAAIED LFGQLAKRLE AEGFTRFIVA GGETSGAVTQ ALAIDGFTIR PQIAPGVPWV RGIGKPLSLA LKSGNFGTEA FFF
Validated NT Sequence
tcaaagaaga aagcctcagt gccgaaattt ccggatttca gtgcaagcga taatggtttt ccgatcccgc gtacccaggg aacgccgggc gcaatctggg gcctgatggt aaacccatcg attgccaaag cctgtgtcac agccccggat gtttctccgc ctgcaacaat gaagcgcgta aacccttcgg cttcgagacg tttcgcaagc tgcccgaaca aatcttcgat ggcagcgctt gcacgttcgc caccaaatcg ctcctgcact gctttcagtg cttccggcgg catggttgca taaacgagcg gcgccaaagc atcgccagac tgtgcgagca cccattcggc cagaacatcg atataggccg cgtcgtttat ggccttctcg acatccatgg ccagcgcggg ggcctttgcc ttgtatgcgg ccacctgcgc attggtcatc tgggagcagg aaccggagag gatgaccgtg cgtcccttca caggcgcgcc ggcagcttca gccgccgcaa cattcttgcc gcgcagttgt gtccatgcgc gcgccatccc gtctgcaagg cccgagccgc ccgtaaccag cttcatttgc gatacggcac gcccaagcgt ttctatatgc tggtcgttca gggcgtcgag gacggcatag cggatgcctt cggactgaag cttcctgagt gcgtcgcgca ctgcatcgct gccctgatcg acgatagtgc tggaaatatt tcctgcacgt ccgcgcgact gggattccat tacccgcatg atgttgctgt cggtcatggg cgtgacgggg tggtttcgca tcccggtttc gctcaacaaa acgccgttca caaacagata gccattatag atggtacggc cattgacggg cagggcaggg cagatgacgg tgaattcttc acccagtgcc tcaagcaatg catcggtgac aggcccgata ttgccttttg gcgtgctgtc gaaggtggag caatatttga agaaaaattg ctggcagttg tttttttgca gccatttcag cgccgcgagg ctttcggcaa ttgcttgccc ggtcgggcag gaacgggtct tcaggctgat gacgaccgca tccgcatcca cggcaaggtc gtccgctgga acgccattca gctgaatggt gcgcaggccg ttgccaacca gaaatcctgc gatatcggtt gctccggtaa aatcgtctgc aatgacgcca ag
Expected Protein Sequence
MAHHHHHHMR LGVIADDFTG ATDIAGFLVG NGLRTIQLNG VPADDLAVDA DAVVISLKTR SCPTGQAIAE SLAALKWLQK NNCQQFFFKY CSTFDSTPKG NIGPVTDALL EALGEEFTVI CPALPVNGRT IYNGYLFVNG VLLSETGMRN HPVTPMTDSN IMRVMESQSR GRAGNISSTI VDQGSDAVRD ALRKLQSEGI RYAVLDALND QHIETLGRAV SQMKLVTGGS GLADGMARAW TQLRGKNVAA AEAAGAPVKG RTVILSGSCS QMTNAQVAAY KAKAPALAMD VEKAINDAAY IDVLAEWVLA QSGDALAPLV YATMPPEALK AVQERFGGER ASAAIEDLFG QLAKRLEAEG FTRFIVAGGE TSGAVTQALA IDGFTIRPQI APGVPWVRGI GKPLSLALKS GNFGTEAFFF EAQKIANQEG DK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgcc ttggcgtcat tgcagacgat tttaccggag caaccgatat cgcaggattt ctggttggca acggcctgcg caccattcag ctgaatggcg ttccagcgga cgaccttgcc gtggatgcgg atgcggtcgt catcagcctg aagacccgtt cctgcccgac cgggcaagca attgccgaaa gcctcgcggc gctgaaatgg ctgcaaaaaa acaactgcca gcaatttttc ttcaaatatt gctccacctt cgacagcacg ccaaaaggca atatcgggcc tgtcaccgat gcattgcttg aggcactggg tgaagaattc accgtcatct gccctgccct gcccgtcaat ggccgtacca tctataatgg ctatctgttt gtgaacggcg ttttgttgag cgaaaccggg atgcgaaacc accccgtcac gcccatgacc gacagcaaca tcatgcgggt aatggaatcc cagtcgcgcg gacgtgcagg aaatatttcc agcactatcg tcgatcaggg cagcgatgca gtgcgcgacg cactcaggaa gcttcagtcc gaaggcatcc gctatgccgt cctcgacgcc ctgaacgacc agcatataga aacgcttggg cgtgccgtat cgcaaatgaa gctggttacg ggcggctcgg gccttgcaga cgggatggcg cgcgcatgga cacaactgcg cggcaagaat gttgcggcgg ctgaagctgc cggcgcgcct gtgaagggac gcacggtcat cctctccggt tcctgctccc agatgaccaa tgcgcaggtg gccgcataca aggcaaaggc ccccgcgctg gccatggatg tcgagaaggc cataaacgac gcggcctata tcgatgttct ggccgaatgg gtgctcgcac agtctggcga tgctttggcg ccgctcgttt atgcaaccat gccgccggaa gcactgaaag cagtgcagga gcgatttggt ggcgaacgtg caagcgctgc catcgaagat ttgttcgggc agcttgcgaa acgtctcgaa gccgaagggt ttacgcgctt cattgttgca ggcggagaaa catccggggc tgtgacacag gctttggcaa tcgatgggtt taccatcagg ccccagattg cgcccggcgt tccctgggta cgcgggatcg gaaaaccatt atcgcttgca ctgaaatccg gaaatttcgg cactgaggct ttcttctttg aagcccaaaa gattgccaat caggaaggag ataaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for BrsuA.18082.a.B1.PS01859
PURIFICATION DATe: 7/1/2013
CONCENTRATION: 31mg/ml
OBSERVED MW: 45kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 63
Protocol Notes
notes unavailable
Validated AA Sequence
MRNHPVTPMT DSNIMRVMES QSRGRAGNIS STIVDQGSDA VRDALRKLQS EGIRYAVLDA LNDQHIETLG RAVSQMKLVT GGSGLADGMA RAWTQLRGKN VAAAEAAGAP VKGRTVILSG SCSQMTNAQV AAYKAKAPAL AMDVEKAIND AAYIDVLAEW VLAQSGDALA PLVYATMPPE ALKAVQERFG GERASAAIED LFGQLAKRLE AEGFTRFIVA GGETSGAVTQ ALAIDGFTIR PQIAPGVPWV RGIGKPLSLA LKSGNFGTEA FFF
Validated NT Sequence
tcaaagaaga aagcctcagt gccgaaattt ccggatttca gtgcaagcga taatggtttt ccgatcccgc gtacccaggg aacgccgggc gcaatctggg gcctgatggt aaacccatcg attgccaaag cctgtgtcac agccccggat gtttctccgc ctgcaacaat gaagcgcgta aacccttcgg cttcgagacg tttcgcaagc tgcccgaaca aatcttcgat ggcagcgctt gcacgttcgc caccaaatcg ctcctgcact gctttcagtg cttccggcgg catggttgca taaacgagcg gcgccaaagc atcgccagac tgtgcgagca cccattcggc cagaacatcg atataggccg cgtcgtttat ggccttctcg acatccatgg ccagcgcggg ggcctttgcc ttgtatgcgg ccacctgcgc attggtcatc tgggagcagg aaccggagag gatgaccgtg cgtcccttca caggcgcgcc ggcagcttca gccgccgcaa cattcttgcc gcgcagttgt gtccatgcgc gcgccatccc gtctgcaagg cccgagccgc ccgtaaccag cttcatttgc gatacggcac gcccaagcgt ttctatatgc tggtcgttca gggcgtcgag gacggcatag cggatgcctt cggactgaag cttcctgagt gcgtcgcgca ctgcatcgct gccctgatcg acgatagtgc tggaaatatt tcctgcacgt ccgcgcgact gggattccat tacccgcatg atgttgctgt cggtcatggg cgtgacgggg tggtttcgca tcccggtttc gctcaacaaa acgccgttca caaacagata gccattatag atggtacggc cattgacggg cagggcaggg cagatgacgg tgaattcttc acccagtgcc tcaagcaatg catcggtgac aggcccgata ttgccttttg gcgtgctgtc gaaggtggag caatatttga agaaaaattg ctggcagttg tttttttgca gccatttcag cgccgcgagg ctttcggcaa ttgcttgccc ggtcgggcag gaacgggtct tcaggctgat gacgaccgca tccgcatcca cggcaaggtc gtccgctgga acgccattca gctgaatggt gcgcaggccg ttgccaacca gaaatcctgc gatatcggtt gctccggtaa aatcgtctgc aatgacgcca ag
Expressed Protein Sequence
MAHHHHHHMR LGVIADDFTG ATDIAGFLVG NGLRTIQLNG VPADDLAVDA DAVVISLKTR SCPTGQAIAE SLAALKWLQK NNCQQFFFKY CSTFDSTPKG NIGPVTDALL EALGEEFTVI CPALPVNGRT IYNGYLFVNG VLLSETGMRN HPVTPMTDSN IMRVMESQSR GRAGNISSTI VDQGSDAVRD ALRKLQSEGI RYAVLDALND QHIETLGRAV SQMKLVTGGS GLADGMARAW TQLRGKNVAA AEAAGAPVKG RTVILSGSCS QMTNAQVAAY KAKAPALAMD VEKAINDAAY IDVLAEWVLA QSGDALAPLV YATMPPEALK AVQERFGGER ASAAIEDLFG QLAKRLEAEG FTRFIVAGGE TSGAVTQALA IDGFTIRPQI APGVPWVRGI GKPLSLALKS GNFGTEAFFF EAQKIANQEG DK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCGCC TTGGCGTCAT TGCAGACGAT TTTACCGGAG CAACCGATAT CGCAGGATTT CTGGTTGGCA ACGGCCTGCG CACCATTCAG CTGAATGGCG TTCCAGCGGA CGACCTTGCC GTGGATGCGG ATGCGGTCGT CATCAGCCTG AAGACCCGTT CCTGCCCGAC CGGGCAAGCA ATTGCCGAAA GCCTCGCGGC GCTGAAATGG CTGCAAAAAA ACAACTGCCA GCAATTTTTC TTCAAATATT GCTCCACCTT CGACAGCACG CCAAAAGGCA ATATCGGGCC TGTCACCGAT GCATTGCTTG AGGCACTGGG TGAAGAATTC ACCGTCATCT GCCCTGCCCT GCCCGTCAAT GGCCGTACCA TCTATAATGG CTATCTGTTT GTGAACGGCG TTTTGTTGAG CGAAACCGGG ATGCGAAACC ACCCCGTCAC GCCCATGACC GACAGCAACA TCATGCGGGT AATGGAATCC CAGTCGCGCG GACGTGCAGG AAATATTTCC AGCACTATCG TCGATCAGGG CAGCGATGCA GTGCGCGACG CACTCAGGAA GCTTCAGTCC GAAGGCATCC GCTATGCCGT CCTCGACGCC CTGAACGACC AGCATATAGA AACGCTTGGG CGTGCCGTAT CGCAAATGAA GCTGGTTACG GGCGGCTCGG GCCTTGCAGA CGGGATGGCG CGCGCATGGA CACAACTGCG CGGCAAGAAT GTTGCGGCGG CTGAAGCTGC CGGCGCGCCT GTGAAGGGAC GCACGGTCAT CCTCTCCGGT TCCTGCTCCC AGATGACCAA TGCGCAGGTG GCCGCATACA AGGCAAAGGC CCCCGCGCTG GCCATGGATG TCGAGAAGGC CATAAACGAC GCGGCCTATA TCGATGTTCT GGCCGAATGG GTGCTCGCAC AGTCTGGCGA TGCTTTGGCG CCGCTCGTTT ATGCAACCAT GCCGCCGGAA GCACTGAAAG CAGTGCAGGA GCGATTTGGT GGCGAACGTG CAAGCGCTGC CATCGAAGAT TTGTTCGGGC AGCTTGCGAA ACGTCTCGAA GCCGAAGGGT TTACGCGCTT CATTGTTGCA GGCGGAGAAA CATCCGGGGC TGTGACACAG GCTTTGGCAA TCGATGGGTT TACCATCAGG CCCCAGATTG CGCCCGGCGT TCCCTGGGTA CGCGGGATCG GAAAACCATT ATCGCTTGCA CTGAAATCCG GAAATTTCGG CACTGAGGCT TTCTTCTTTG AAGCCCAAAA GATTGCCAAT CAGGAAGGAG ATAAATGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT
Details for BrsuA.18082.a.B1.PS01860
PURIFICATION DATe: 7/1/2013
CONCENTRATION: 46.5mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 63
Protocol Notes
notes unavailable
Validated AA Sequence
MRNHPVTPMT DSNIMRVMES QSRGRAGNIS STIVDQGSDA VRDALRKLQS EGIRYAVLDA LNDQHIETLG RAVSQMKLVT GGSGLADGMA RAWTQLRGKN VAAAEAAGAP VKGRTVILSG SCSQMTNAQV AAYKAKAPAL AMDVEKAIND AAYIDVLAEW VLAQSGDALA PLVYATMPPE ALKAVQERFG GERASAAIED LFGQLAKRLE AEGFTRFIVA GGETSGAVTQ ALAIDGFTIR PQIAPGVPWV RGIGKPLSLA LKSGNFGTEA FFF
Validated NT Sequence
tcaaagaaga aagcctcagt gccgaaattt ccggatttca gtgcaagcga taatggtttt ccgatcccgc gtacccaggg aacgccgggc gcaatctggg gcctgatggt aaacccatcg attgccaaag cctgtgtcac agccccggat gtttctccgc ctgcaacaat gaagcgcgta aacccttcgg cttcgagacg tttcgcaagc tgcccgaaca aatcttcgat ggcagcgctt gcacgttcgc caccaaatcg ctcctgcact gctttcagtg cttccggcgg catggttgca taaacgagcg gcgccaaagc atcgccagac tgtgcgagca cccattcggc cagaacatcg atataggccg cgtcgtttat ggccttctcg acatccatgg ccagcgcggg ggcctttgcc ttgtatgcgg ccacctgcgc attggtcatc tgggagcagg aaccggagag gatgaccgtg cgtcccttca caggcgcgcc ggcagcttca gccgccgcaa cattcttgcc gcgcagttgt gtccatgcgc gcgccatccc gtctgcaagg cccgagccgc ccgtaaccag cttcatttgc gatacggcac gcccaagcgt ttctatatgc tggtcgttca gggcgtcgag gacggcatag cggatgcctt cggactgaag cttcctgagt gcgtcgcgca ctgcatcgct gccctgatcg acgatagtgc tggaaatatt tcctgcacgt ccgcgcgact gggattccat tacccgcatg atgttgctgt cggtcatggg cgtgacgggg tggtttcgca tcccggtttc gctcaacaaa acgccgttca caaacagata gccattatag atggtacggc cattgacggg cagggcaggg cagatgacgg tgaattcttc acccagtgcc tcaagcaatg catcggtgac aggcccgata ttgccttttg gcgtgctgtc gaaggtggag caatatttga agaaaaattg ctggcagttg tttttttgca gccatttcag cgccgcgagg ctttcggcaa ttgcttgccc ggtcgggcag gaacgggtct tcaggctgat gacgaccgca tccgcatcca cggcaaggtc gtccgctgga acgccattca gctgaatggt gcgcaggccg ttgccaacca gaaatcctgc gatatcggtt gctccggtaa aatcgtctgc aatgacgcca ag
Expressed Protein Sequence
MAHHHHHHMR LGVIADDFTG ATDIAGFLVG NGLRTIQLNG VPADDLAVDA DAVVISLKTR SCPTGQAIAE SLAALKWLQK NNCQQFFFKY CSTFDSTPKG NIGPVTDALL EALGEEFTVI CPALPVNGRT IYNGYLFVNG VLLSETGMRN HPVTPMTDSN IMRVMESQSR GRAGNISSTI VDQGSDAVRD ALRKLQSEGI RYAVLDALND QHIETLGRAV SQMKLVTGGS GLADGMARAW TQLRGKNVAA AEAAGAPVKG RTVILSGSCS QMTNAQVAAY KAKAPALAMD VEKAINDAAY IDVLAEWVLA QSGDALAPLV YATMPPEALK AVQERFGGER ASAAIEDLFG QLAKRLEAEG FTRFIVAGGE TSGAVTQALA IDGFTIRPQI APGVPWVRGI GKPLSLALKS GNFGTEAFFF EAQKIANQEG DK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCGCC TTGGCGTCAT TGCAGACGAT TTTACCGGAG CAACCGATAT CGCAGGATTT CTGGTTGGCA ACGGCCTGCG CACCATTCAG CTGAATGGCG TTCCAGCGGA CGACCTTGCC GTGGATGCGG ATGCGGTCGT CATCAGCCTG AAGACCCGTT CCTGCCCGAC CGGGCAAGCA ATTGCCGAAA GCCTCGCGGC GCTGAAATGG CTGCAAAAAA ACAACTGCCA GCAATTTTTC TTCAAATATT GCTCCACCTT CGACAGCACG CCAAAAGGCA ATATCGGGCC TGTCACCGAT GCATTGCTTG AGGCACTGGG TGAAGAATTC ACCGTCATCT GCCCTGCCCT GCCCGTCAAT GGCCGTACCA TCTATAATGG CTATCTGTTT GTGAACGGCG TTTTGTTGAG CGAAACCGGG ATGCGAAACC ACCCCGTCAC GCCCATGACC GACAGCAACA TCATGCGGGT AATGGAATCC CAGTCGCGCG GACGTGCAGG AAATATTTCC AGCACTATCG TCGATCAGGG CAGCGATGCA GTGCGCGACG CACTCAGGAA GCTTCAGTCC GAAGGCATCC GCTATGCCGT CCTCGACGCC CTGAACGACC AGCATATAGA AACGCTTGGG CGTGCCGTAT CGCAAATGAA GCTGGTTACG GGCGGCTCGG GCCTTGCAGA CGGGATGGCG CGCGCATGGA CACAACTGCG CGGCAAGAAT GTTGCGGCGG CTGAAGCTGC CGGCGCGCCT GTGAAGGGAC GCACGGTCAT CCTCTCCGGT TCCTGCTCCC AGATGACCAA TGCGCAGGTG GCCGCATACA AGGCAAAGGC CCCCGCGCTG GCCATGGATG TCGAGAAGGC CATAAACGAC GCGGCCTATA TCGATGTTCT GGCCGAATGG GTGCTCGCAC AGTCTGGCGA TGCTTTGGCG CCGCTCGTTT ATGCAACCAT GCCGCCGGAA GCACTGAAAG CAGTGCAGGA GCGATTTGGT GGCGAACGTG CAAGCGCTGC CATCGAAGAT TTGTTCGGGC AGCTTGCGAA ACGTCTCGAA GCCGAAGGGT TTACGCGCTT CATTGTTGCA GGCGGAGAAA CATCCGGGGC TGTGACACAG GCTTTGGCAA TCGATGGGTT TACCATCAGG CCCCAGATTG CGCCCGGCGT TCCCTGGGTA CGCGGGATCG GAAAACCATT ATCGCTTGCA CTGAAATCCG GAAATTTCGG CACTGAGGCT TTCTTCTTTG AAGCCCAAAA GATTGCCAAT CAGGAAGGAG ATAAATGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT