BrsuA.17377.b

Transcriptional regulator, LacI family

CENTER ID: BrsuA.17377.b
ORGANISM: Brucella suis 1330
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuA.17377.b.B1.GE36811 Full length( BrsuA.17377.b ) 1 203
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuA.17377.b.B1.PS01933 Full length( BrsuA.17377.b ) 1 203

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruSui107850_0213

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQELTASAMP FARVAGDANV HPTDSVTIDD EAAAVDLMNY LLELGHRKIA IVIGDPTHRS AELRLEGYRR ALREAGIEGN SDYEVQGGFS FASGLVAGRK LLSLPNRPTA IFASNDDMAA AVMQVAYDAG IAVPDDLTVV GFDDSAIATM VSPQITTVRQ PIFEMTRDAA DMLLRQMESG ETSPAQRIDY KLIVRQSSAR VPA
NT Sequence
TTGCAGGAAT TGACGGCCTC GGCCATGCCT TTCGCGCGTG TGGCGGGGGA TGCGAATGTG CATCCGACCG ACAGCGTCAC CATCGACGAC GAGGCGGCGG CTGTCGATCT GATGAACTAT CTCCTGGAAC TCGGTCATCG AAAGATCGCC ATCGTGATTG GCGATCCGAC ACACCGTTCT GCCGAATTGC GCCTGGAAGG CTATCGCCGC GCGCTGCGTG AGGCGGGGAT CGAAGGCAAT TCCGATTATG AGGTGCAGGG CGGGTTCAGT TTTGCAAGCG GCCTTGTGGC GGGCCGCAAG CTGCTTTCGC TCCCGAACAG GCCGACCGCC ATCTTCGCCT CCAATGACGA TATGGCAGCC GCCGTGATGC AGGTCGCCTA TGATGCCGGG ATCGCCGTTC CCGATGATCT TACGGTCGTC GGTTTTGACG ATAGTGCGAT TGCAACTATG GTCTCGCCGC AGATCACCAC TGTTCGCCAG CCGATTTTCG AGATGACGCG GGATGCCGCC GATATGCTTT TGCGCCAGAT GGAGAGCGGG GAAACCTCGC CTGCACAGCG GATCGACTAT AAGCTCATCG TTCGCCAGTC TTCCGCCAGA GTTCCTGCC
Details for BrsuA.17377.b.B1.GE36811
HARVESTED ON: 3/27/2013
SEQUENCED ON: 3/30/2013
EXPECTED MW: 23kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML QELTASAMPF ARVAGDANVH PTDSVTIDDE AAAVDLMNYL LELGHRKIAI VIGDPTHRSA ELRLEGYRRA LREAGIEGNS DYEVQGGFSF ASGLVAGRKL LSLPNRPTAI FASNDDMAAA VMQVAYDAGI AVPDDLTVVG FDDSAIATMV SPQITTVRQP IFEMTRDAAD MLLRQMESGE TSPAQRIDYK LIVRQSSARV PA
Validated NT Sequence
atcttactca cttaggcagg aactctggcg gaagactggc gaacgatgag cttatagtcg atccgctgtg caggcgaggt ttccccgctc tccatctggc gcaaaagcat atcggcggca tcccgcgtca tctcgaaaat cggctggcga acagtggtga tctgcggcga gaccatagtt gcaatcgcac tatcgtcaaa accgacgacc gtaagatcat cgggaacggc gatcccggca tcataggcga cctgcatcac ggcggctgcc atatcgtcat tggaggcgaa gatggcggtc ggcctgttcg ggagcgaaag cagcttgcgg cccgccacaa ggccgcttgc aaaactgaac ccgccctgca cctcataatc ggaattgcct tcgatccccg cctcacgcag cgcgcggcga tagccttcca ggcgcaattc ggcagaacgg tgtgtcggat cgccaatcac gatggcgatc tttcgatgac cgagttccag gagatagttc atcagatcga cagccgccgc ctcgtcgtcg atggtgacgc tgtcggtcgg atgcacattc gcatcccccg ccacacgcgc gaaaggcatg gccgaggccg tcaattcctg caacatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMQ ELTASAMPFA RVAGDANVHP TDSVTIDDEA AAVDLMNYLL ELGHRKIAIV IGDPTHRSAE LRLEGYRRAL REAGIEGNSD YEVQGGFSFA SGLVAGRKLL SLPNRPTAIF ASNDDMAAAV MQVAYDAGIA VPDDLTVVGF DDSAIATMVS PQITTVRQPI FEMTRDAADM LLRQMESGET SPAQRIDYKL IVRQSSARVP A
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgcagg aattgacggc ctcggccatg cctttcgcgc gtgtggcggg ggatgcgaat gtgcatccga ccgacagcgt caccatcgac gacgaggcgg cggctgtcga tctgatgaac tatctcctgg aactcggtca tcgaaagatc gccatcgtga ttggcgatcc gacacaccgt tctgccgaat tgcgcctgga aggctatcgc cgcgcgctgc gtgaggcggg gatcgaaggc aattccgatt atgaggtgca gggcgggttc agttttgcaa gcggccttgt ggcgggccgc aagctgcttt cgctcccgaa caggccgacc gccatcttcg cctccaatga cgatatggca gccgccgtga tgcaggtcgc ctatgatgcc gggatcgccg ttcccgatga tcttacggtc gtcggttttg acgatagtgc gattgcaact atggtctcgc cgcagatcac cactgttcgc cagccgattt tcgagatgac gcgggatgcc gccgatatgc ttttgcgcca gatggagagc ggggaaacct cgcctgcaca gcggatcgac tataagctca tcgttcgcca gtcttccgcc agagttcctg cctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for BrsuA.17377.b.B1.PS01933
PURIFICATION DATe: 11/13/2013
CONCENTRATION: 41.9mg/ml
OBSERVED MW: 25kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML QELTASAMPF ARVAGDANVH PTDSVTIDDE AAAVDLMNYL LELGHRKIAI VIGDPTHRSA ELRLEGYRRA LREAGIEGNS DYEVQGGFSF ASGLVAGRKL LSLPNRPTAI FASNDDMAAA VMQVAYDAGI AVPDDLTVVG FDDSAIATMV SPQITTVRQP IFEMTRDAAD MLLRQMESGE TSPAQRIDYK LIVRQSSARV PA
Validated NT Sequence
atcttactca cttaggcagg aactctggcg gaagactggc gaacgatgag cttatagtcg atccgctgtg caggcgaggt ttccccgctc tccatctggc gcaaaagcat atcggcggca tcccgcgtca tctcgaaaat cggctggcga acagtggtga tctgcggcga gaccatagtt gcaatcgcac tatcgtcaaa accgacgacc gtaagatcat cgggaacggc gatcccggca tcataggcga cctgcatcac ggcggctgcc atatcgtcat tggaggcgaa gatggcggtc ggcctgttcg ggagcgaaag cagcttgcgg cccgccacaa ggccgcttgc aaaactgaac ccgccctgca cctcataatc ggaattgcct tcgatccccg cctcacgcag cgcgcggcga tagccttcca ggcgcaattc ggcagaacgg tgtgtcggat cgccaatcac gatggcgatc tttcgatgac cgagttccag gagatagttc atcagatcga cagccgccgc ctcgtcgtcg atggtgacgc tgtcggtcgg atgcacattc gcatcccccg ccacacgcgc gaaaggcatg gccgaggccg tcaattcctg caacatatgg tggtggtggt ggtgagccat
Expressed Protein Sequence
MAHHHHHHMQ ELTASAMPFA RVAGDANVHP TDSVTIDDEA AAVDLMNYLL ELGHRKIAIV IGDPTHRSAE LRLEGYRRAL REAGIEGNSD YEVQGGFSFA SGLVAGRKLL SLPNRPTAIF ASNDDMAAAV MQVAYDAGIA VPDDLTVVGF DDSAIATMVS PQITTVRQPI FEMTRDAADM LLRQMESGET SPAQRIDYKL IVRQSSARVP A
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTTGCAGG AATTGACGGC CTCGGCCATG CCTTTCGCGC GTGTGGCGGG GGATGCGAAT GTGCATCCGA CCGACAGCGT CACCATCGAC GACGAGGCGG CGGCTGTCGA TCTGATGAAC TATCTCCTGG AACTCGGTCA TCGAAAGATC GCCATCGTGA TTGGCGATCC GACACACCGT TCTGCCGAAT TGCGCCTGGA AGGCTATCGC CGCGCGCTGC GTGAGGCGGG GATCGAAGGC AATTCCGATT ATGAGGTGCA GGGCGGGTTC AGTTTTGCAA GCGGCCTTGT GGCGGGCCGC AAGCTGCTTT CGCTCCCGAA CAGGCCGACC GCCATCTTCG CCTCCAATGA CGATATGGCA GCCGCCGTGA TGCAGGTCGC CTATGATGCC GGGATCGCCG TTCCCGATGA TCTTACGGTC GTCGGTTTTG ACGATAGTGC GATTGCAACT ATGGTCTCGC CGCAGATCAC CACTGTTCGC CAGCCGATTT TCGAGATGAC GCGGGATGCC GCCGATATGC TTTTGCGCCA GATGGAGAGC GGGGAAACCT CGCCTGCACA GCGGATCGAC TATAAGCTCA TCGTTCGCCA GTCTTCCGCC AGAGTTCCTG CCTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT