BrsuA.17369.a

Flagellum-specific ATP synthase FliI

CENTER ID: BrsuA.17369.a
ORGANISM: Brucella suis 1330
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuA.17369.a.B1.GE36809 Full length( BrsuA.17369.a ) 1 422
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruSui107850_2350
BV-BRC: fig|204722.5.peg.2350
RefSeq: NP_699332.1
RefSeq: YP_005613842.1
UniProt: A0A0H3GE83

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTPDLPLTRL AAFVRQQTIA PEPLTGIGGT VSDVSRSSIA VRGLSRDARL GDTVAIRAGE GAPSLAEIIR VADLQVLVKP FDDRIMPSLG AAVFEEGPLR IRPAPEWRGR VINALGNAID GKGALKLGTR PMAAESLAPA ALRRARVDRG LRTGVNVIDI FTPLCFGQRI GIFAGSGVGK STLLAMMTRA ADFDTVVLAL TGERGREVRE MLEETMAGHL GKTITVVATG DESPMMRRLA PNTATAIAEY FRDLGQNVLL IVDSVTRFAH AAREVAIAAE EPPVARGYPP SVFSQLPRLL ERAGPGSAEA GGSIAGIYSV LVDGDDHNDP ASVSRLAKHN WTPEQRKLVM QLRSMVARFE ETRDLRAIGA YQKGHDGLLD QAVDFVPRIY EALQQSPETG LSDDPYNDLA AALRGDRQAQ NA
NT Sequence
ATGACGCCTG ATCTGCCACT TACCCGCCTT GCTGCTTTTG TTCGCCAGCA AACAATAGCG CCAGAGCCGC TCACCGGTAT CGGGGGCACG GTGAGTGACG TTTCGCGTTC GTCTATTGCA GTGCGTGGCC TTTCGCGCGA TGCGCGGCTT GGCGATACGG TGGCAATCCG CGCTGGCGAG GGGGCGCCGA GCCTTGCCGA AATCATCCGT GTTGCCGATT TGCAGGTGCT GGTGAAACCG TTCGATGACC GGATCATGCC TTCGCTGGGG GCAGCGGTTT TCGAGGAAGG CCCGTTGCGC ATCCGCCCGG CGCCGGAATG GCGGGGCCGG GTCATCAATG CGCTTGGAAA TGCCATTGAC GGAAAGGGCG CGCTGAAACT GGGCACCCGC CCCATGGCGG CAGAAAGCCT TGCTCCGGCG GCTTTGCGCC GTGCGCGGGT GGATCGCGGC TTGCGTACCG GCGTTAATGT GATCGATATT TTCACGCCGC TCTGTTTTGG CCAGCGCATC GGCATTTTCG CAGGCTCCGG TGTCGGCAAA TCAACGCTTC TCGCCATGAT GACGCGCGCT GCCGATTTCG ATACGGTCGT GCTGGCGCTG ACGGGCGAGC GCGGGCGCGA AGTGCGCGAA ATGCTGGAAG AAACCATGGC CGGGCATTTG GGCAAGACCA TAACTGTGGT GGCGACCGGC GATGAAAGCC CGATGATGCG CCGCCTTGCG CCCAATACGG CGACGGCGAT TGCCGAATAT TTCCGCGATC TTGGCCAGAA TGTTCTGTTG ATCGTCGATT CCGTCACACG CTTTGCCCAT GCGGCACGCG AAGTGGCCAT TGCTGCCGAG GAGCCGCCGG TTGCACGCGG TTATCCGCCA AGTGTTTTCA GCCAGTTGCC CCGCCTTCTG GAGCGTGCCG GTCCCGGCAG TGCCGAAGCG GGTGGCAGCA TCGCCGGTAT TTATTCCGTG CTGGTGGATG GTGACGATCA TAACGATCCG GCTTCCGTTT CGCGCCTTGC CAAACACAAC TGGACGCCGG AACAGCGTAA GCTGGTGATG CAATTGCGCA GCATGGTTGC CCGGTTCGAG GAAACGCGCG ACCTGCGCGC CATTGGCGCT TATCAGAAAG GCCATGACGG GCTTCTGGAC CAGGCGGTCG ATTTTGTGCC GCGTATTTAT GAAGCTTTAC AGCAATCACC GGAAACCGGC CTATCGGACG ATCCCTATAA CGACCTTGCC GCAGCATTGC GCGGCGACAG ACAGGCGCAA AACGCA
Details for BrsuA.17369.a.B1.GE36809
HARVESTED ON: 3/27/2013
SEQUENCED ON: 3/30/2013
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 82
PERCENT COVERAGE: 89
Validated AA Sequence
MAHHHHHHMT PDLPLTRLAA FVRQQTIAPE PLTGIGGTVS DVSRSSIAVR GLSRDARLGD TVAIRAGEGA PSLAEIIRVA DLQVLVKPFD DRIMPSLGAA VFEEGPLRIR PAPEWRGRVI NALGNAIDGK GALKLGTRPM AAESLAPAAL RRARVDRGLR TGVNVIDIFT PLCFGQRIGI FAGSGVGKST LLAMMTRAAD FDTVVLALTG ERGREVREML EETMAGHLGK TITVVATGDE SPMMRRLAPN TATAIAEYFR DLGQNVLLIV DSVTRFAHAA REVXIAAEEP PVARGYPPSV FSQLPRLLER AXPXSAXXGX XSXXLXRAXX XXIXXXLXFX XXXXXXXXXX TXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXX
Validated NT Sequence
cnnnnnnnat tttgntnnnt ttaagaagga ganntaccat ggctcaccac caccaccacc atatgacgcc tgatctgcca cttacccgcc ttgctgcttt tgttcgccag caaacaatag cgccagagcc gctcaccggt atcgggggca cggtgagtga cgtttcgcgt tcgtctattg cagtgcgtgg cctttcgcgc gatgcgcggc ttggcgatac ggtggcaatc cgcgctggcg agggggcgcc gagccttgcc gaaatcatcc gtgttgccga tttgcaggtg ctggtgaaac cgttcgatga ccggatcatg ccttcgctgg gggcagcggt tttcgaggaa ggcccgttgc gcatccgccc ggcgccggaa tggcggggcc gggtcatcaa tgcgcttgga aatgccattg acggaaaggg cgcgctgaaa ctgggcaccc gccccatggc ggcagaaagc cttgctccgg cggctttgcg ccgtgcgcgg gtggatcgcg gcttgcgtac cggcgttaat gtgatcgata ttttcacgcc gctctgtttt ggccagcgca tcggcatttt cgcaggctcc ggtgtcggca aatcaacgct tctcgccatg atgacgcgcg ctgccgattt cgatacggtc gtgctggcgc tgacgggcga gcgcgggcgc gaagtgcgcg aaatgctgga agaaaccatg gccgggcatt tgggcaagac cataactgtg gtggcgaccg gcgatgaaag cccgatgatg cgccgccttg cgcccaatac ggcgacggcg attgccgaat atttccgcga tcttggccag aatgttctgt tgatcgtcga ttccgtcaca cgctttgccc atgcggcacg cgaagtgnnc attgctgccg aggagccgcc ggttgcacgc ggttatccgc cnagtgtttt cagccagttg ccccgccttc tngagcgtgc cngtccnngc agtgccnaan cgggngnnnc atcnnnnnnn ttantncgng ctgnngnngn nnnnatcnna nancnncttn cntttnnnnn nnnnnnnnan ngnnnncnna nnnnnnnnac tngnnanncn nnnnnnnnnn nnnnnnnnnn nnannnnnnc nnnnnncngn cnnnnnannn nnnnngnnnn nnnnnnnnnn nnnnnnnnnn tntnttnnnn nnnnc
Expected Protein Sequence
MAHHHHHHMT PDLPLTRLAA FVRQQTIAPE PLTGIGGTVS DVSRSSIAVR GLSRDARLGD TVAIRAGEGA PSLAEIIRVA DLQVLVKPFD DRIMPSLGAA VFEEGPLRIR PAPEWRGRVI NALGNAIDGK GALKLGTRPM AAESLAPAAL RRARVDRGLR TGVNVIDIFT PLCFGQRIGI FAGSGVGKST LLAMMTRAAD FDTVVLALTG ERGREVREML EETMAGHLGK TITVVATGDE SPMMRRLAPN TATAIAEYFR DLGQNVLLIV DSVTRFAHAA REVAIAAEEP PVARGYPPSV FSQLPRLLER AGPGSAEAGG SIAGIYSVLV DGDDHNDPAS VSRLAKHNWT PEQRKLVMQL RSMVARFEET RDLRAIGAYQ KGHDGLLDQA VDFVPRIYEA LQQSPETGLS DDPYNDLAAA LRGDRQAQNA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacgc ctgatctgcc acttacccgc cttgctgctt ttgttcgcca gcaaacaata gcgccagagc cgctcaccgg tatcgggggc acggtgagtg acgtttcgcg ttcgtctatt gcagtgcgtg gcctttcgcg cgatgcgcgg cttggcgata cggtggcaat ccgcgctggc gagggggcgc cgagccttgc cgaaatcatc cgtgttgccg atttgcaggt gctggtgaaa ccgttcgatg accggatcat gccttcgctg ggggcagcgg ttttcgagga aggcccgttg cgcatccgcc cggcgccgga atggcggggc cgggtcatca atgcgcttgg aaatgccatt gacggaaagg gcgcgctgaa actgggcacc cgccccatgg cggcagaaag ccttgctccg gcggctttgc gccgtgcgcg ggtggatcgc ggcttgcgta ccggcgttaa tgtgatcgat attttcacgc cgctctgttt tggccagcgc atcggcattt tcgcaggctc cggtgtcggc aaatcaacgc ttctcgccat gatgacgcgc gctgccgatt tcgatacggt cgtgctggcg ctgacgggcg agcgcgggcg cgaagtgcgc gaaatgctgg aagaaaccat ggccgggcat ttgggcaaga ccataactgt ggtggcgacc ggcgatgaaa gcccgatgat gcgccgcctt gcgcccaata cggcgacggc gattgccgaa tatttccgcg atcttggcca gaatgttctg ttgatcgtcg attccgtcac acgctttgcc catgcggcac gcgaagtggc cattgctgcc gaggagccgc cggttgcacg cggttatccg ccaagtgttt tcagccagtt gccccgcctt ctggagcgtg ccggtcccgg cagtgccgaa gcgggtggca gcatcgccgg tatttattcc gtgctggtgg atggtgacga tcataacgat ccggcttccg tttcgcgcct tgccaaacac aactggacgc cggaacagcg taagctggtg atgcaattgc gcagcatggt tgcccggttc gaggaaacgc gcgacctgcg cgccattggc gcttatcaga aaggccatga cgggcttctg gaccaggcgg tcgattttgt gccgcgtatt tatgaagctt tacagcaatc accggaaacc ggcctatcgg acgatcccta taacgacctt gccgcagcat tgcgcggcga cagacaggcg caaaacgcat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat