BrsuA.17368.a

ABC transporter, substrate-binding protein

CENTER ID: BrsuA.17368.a
ORGANISM: Brucella suis 1330
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuA.17368.a.B2.GE36808 Mature protein( BrsuA.17368.a ) 23 368
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruSui107850_0016
BV-BRC: fig|204722.5.peg.16
RefSeq: NP_697057.1
RefSeq: YP_005614876.1
UniProt: Q8G3D4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNLKLLSSLA FAATIGFASA AYADITIGVI APLTGPVAAF GDQVKKGAET AVEVINKAGG IKGEKVVLKF ADDAGEPKQG VSAANQIVGD GIKFVVGPVT TGVAVPVSDV LSENGVLMVT PTATGPDLTA RGLENVFRTC GRDDQQAEVM ADYVLKNMKD KKVAVIHDKG AYGKGLADAF KAAINKGGIT EVHYDSVTPG DKDFSALVTK LKSAGAEVVY FGGYHAEGGL LSRQLHDAGM QALVLGGEGL SNTEYWAIGG TNAQGTLFTN AKDATKNPAA KDAIQALKAK NIPAEAFTMN AYAAVEVIKA GIERAGSTDD SAAVAKALHD GKPIETAIGT LTYSETGDLS SPSFDIFKWD DGKIVGLE
NT Sequence
ATGAACCTGA AACTTCTGTC CAGCTTGGCT TTTGCCGCTA CCATCGGCTT TGCCAGCGCC GCTTACGCAG ACATCACCAT CGGTGTGATT GCACCGCTGA CGGGCCCGGT TGCCGCTTTC GGCGATCAGG TGAAAAAGGG CGCGGAAACC GCCGTGGAAG TCATCAACAA GGCTGGCGGC ATCAAGGGTG AGAAAGTCGT CCTGAAGTTT GCCGACGATG CGGGCGAGCC GAAGCAGGGC GTTTCCGCTG CCAACCAGAT CGTTGGCGAT GGCATCAAGT TTGTCGTCGG CCCGGTTACG ACCGGCGTTG CCGTCCCCGT TTCCGACGTT TTGTCTGAAA ACGGCGTCCT CATGGTCACC CCGACGGCTA CCGGCCCGGA CCTGACCGCG CGCGGCCTGG AAAACGTGTT CCGTACCTGC GGCCGCGACG ACCAGCAGGC TGAAGTCATG GCTGATTACG TCCTGAAAAA CATGAAGGAC AAGAAGGTTG CCGTGATCCA CGACAAGGGC GCTTATGGCA AGGGTCTTGC CGATGCGTTC AAGGCGGCCA TCAACAAGGG CGGCATCACT GAAGTCCATT ATGATTCGGT CACACCGGGC GACAAGGATT TTTCCGCGCT CGTCACCAAG CTGAAGTCTG CCGGTGCTGA AGTGGTCTAT TTCGGCGGCT ACCATGCTGA AGGCGGTCTC CTGTCGCGCC AGCTCCACGA TGCTGGCATG CAGGCCCTGG TGCTCGGCGG CGAAGGCCTC TCCAACACCG AATATTGGGC AATCGGCGGC ACAAACGCAC AGGGTACGCT CTTCACCAAC GCCAAGGATG CCACGAAGAA CCCGGCTGCC AAGGACGCTA TCCAAGCCCT GAAGGCGAAG AACATCCCGG CTGAAGCTTT CACGATGAAC GCCTATGCGG CTGTGGAAGT CATCAAGGCC GGCATCGAAC GCGCCGGTTC GACTGACGAT TCCGCTGCCG TTGCCAAGGC GCTGCATGAC GGCAAGCCGA TCGAAACCGC AATCGGCACG CTGACCTATA GCGAAACCGG CGACCTTAGC TCGCCAAGCT TTGATATCTT CAAGTGGGAT GACGGCAAGA TCGTCGGTCT TGAA
Details for BrsuA.17368.a.B2.GE36808
HARVESTED ON: 3/27/2013
SEQUENCED ON: 3/30/2013
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 95
Validated AA Sequence
MADITIGVIA PLTGPVAAFG DQVKKGAETA VEVINKAGGI KGEKVVLKFA DDAGEPKQGV SAANQIVGDG IKFVVGPVTT GVAVPVSDVL SENGVLMVTP TATGPDLTAR GLENVFRTCG RDDQQAEVMA DYVLKNMKDK KVAVIHDKGA YGKGLADAFK AAINKGGITE VHYDSVTPGD KDFSALVTKL KSAGAEVVYF GGYHAEGGLL SRQLHDAGMQ ALVLGGEGLS NTEYWAIGGT NAQGTLFTNA KDATKNPAAK DAIQALKAKN IPAEAFTMNA YAAVEVIKAG IERAGSTDDS AAVAKALHDG KPIETAIGTL TYSETGDLSS PSFDIFKW
Validated NT Sequence
tcccacttga agatatcaaa gcttggcgag ctaaggtcgc cggtttcgct ataggtcagc gtgccgattg cggtttcgat cggcttgccg tcatgcagcg ccttggcaac ggcagcggaa tcgtcagtcg aaccggcgcg ttcgatgccg gccttgatga cttccacagc cgcataggcg ttcatcgtga aagcttcagc cgggatgttc ttcgccttca gggcttggat agcgtccttg gcagccgggt tcttcgtggc atccttggcg ttggtgaaga gcgtaccctg tgcgtttgtg ccgccgattg cccaatattc ggtgttggag aggccttcgc cgccgagcac cagggcctgc atgccagcat cgtggagctg gcgcgacagg agaccgcctt cagcatggta gccgccgaaa tagaccactt cagcaccggc agacttcagc ttggtgacga gcgcggaaaa atccttgtcg cccggtgtga ccgaatcata atggacttca gtgatgccgc ccttgttgat ggccgccttg aacgcatcgg caagaccctt gccataagcg cccttgtcgt ggatcacggc aaccttcttg tccttcatgt ttttcaggac gtaatcagcc atgacttcag cctgctggtc gtcgcggccg caggtacgga acacgttttc caggccgcgc gcggtcaggt ccgggccggt agccgtcggg gtgaccatga ggacgccgtt ttcagacaaa acgtcggaaa cggggacggc aacgccggtc gtaaccgggc cgacgacaaa cttgatgcca tcgccaacga tctggttggc agcggaaacg ccctgcttcg gctcgcccgc atcgtcggca aacttcagga cgactttctc acccttgatg ccgccagcct tgttgatgac ttccacggcg gtttccgcgc cctttttcac ctgatcgccg aaagcggcaa ccgggcccgt cagcggtgca atcacaccga tggtgatgtc tgccatatgg tggtggtggt ggtgagcca
Expected Protein Sequence
MAHHHHHHAD ITIGVIAPLT GPVAAFGDQV KKGAETAVEV INKAGGIKGE KVVLKFADDA GEPKQGVSAA NQIVGDGIKF VVGPVTTGVA VPVSDVLSEN GVLMVTPTAT GPDLTARGLE NVFRTCGRDD QQAEVMADYV LKNMKDKKVA VIHDKGAYGK GLADAFKAAI NKGGITEVHY DSVTPGDKDF SALVTKLKSA GAEVVYFGGY HAEGGLLSRQ LHDAGMQALV LGGEGLSNTE YWAIGGTNAQ GTLFTNAKDA TKNPAAKDAI QALKAKNIPA EAFTMNAYAA VEVIKAGIER AGSTDDSAAV AKALHDGKPI ETAIGTLTYS ETGDLSSPSF DIFKWDDGKI VGLE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcagaca tcaccatcgg tgtgattgca ccgctgacgg gcccggttgc cgctttcggc gatcaggtga aaaagggcgc ggaaaccgcc gtggaagtca tcaacaaggc tggcggcatc aagggtgaga aagtcgtcct gaagtttgcc gacgatgcgg gcgagccgaa gcagggcgtt tccgctgcca accagatcgt tggcgatggc atcaagtttg tcgtcggccc ggttacgacc ggcgttgccg tccccgtttc cgacgttttg tctgaaaacg gcgtcctcat ggtcaccccg acggctaccg gcccggacct gaccgcgcgc ggcctggaaa acgtgttccg tacctgcggc cgcgacgacc agcaggctga agtcatggct gattacgtcc tgaaaaacat gaaggacaag aaggttgccg tgatccacga caagggcgct tatggcaagg gtcttgccga tgcgttcaag gcggccatca acaagggcgg catcactgaa gtccattatg attcggtcac accgggcgac aaggattttt ccgcgctcgt caccaagctg aagtctgccg gtgctgaagt ggtctatttc ggcggctacc atgctgaagg cggtctcctg tcgcgccagc tccacgatgc tggcatgcag gccctggtgc tcggcggcga aggcctctcc aacaccgaat attgggcaat cggcggcaca aacgcacagg gtacgctctt caccaacgcc aaggatgcca cgaagaaccc ggctgccaag gacgctatcc aagccctgaa ggcgaagaac atcccggctg aagctttcac gatgaacgcc tatgcggctg tggaagtcat caaggccggc atcgaacgcg ccggttcgac tgacgattcc gctgccgttg ccaaggcgct gcatgacggc aagccgatcg aaaccgcaat cggcacgctg acctatagcg aaaccggcga ccttagctcg ccaagctttg atatcttcaa gtgggatgac ggcaagatcg tcggtcttga atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat