BrsuA.17285.b

Amino acid ABC transporter, periplasmic amino acid-binding protein

CENTER ID: BrsuA.17285.b
ORGANISM: Brucella suis 1330
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuA.17285.b.B2.GE36803 Mature protein( BrsuA.17285.b ) 24 245
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruSui107850_0873

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRGFKRIAAC AVIAGFAAIG AARAEEPLKL VIGTEGAYPP FNFINPDGSL SGFDVDIARA LCDEMKAECT FITQEWDGTI PALQAGKFDA YVSSMSITEE RKKQVDFSDK YYNTPPGVAA PKDTDIKGVT KEDLAGKTIG VQVSTTHANY AEQTFTDSTI KAYPTAEEYR LDLANGRLDA VNDDSVTLSE WLKTPDGACC KTVGNLPAGR CNPWCRRWRC LQEGPPGTGR KVQRCHQGYS QEWQV
NT Sequence
ATGCGCGGTT TTAAGCGTAT TGCGGCATGT GCCGTGATTG CGGGTTTTGC GGCTATTGGG GCAGCAAGGG CTGAAGAGCC CCTCAAGCTC GTCATCGGAA CCGAGGGGGC ATATCCGCCA TTTAATTTCA TCAATCCTGA TGGCTCGCTA TCGGGCTTCG ATGTGGATAT TGCTCGGGCG CTTTGCGATG AGATGAAGGC GGAGTGCACA TTCATTACGC AGGAATGGGA TGGCACCATC CCGGCATTGC AGGCAGGCAA GTTCGACGCC TATGTGTCTT CCATGTCGAT TACGGAAGAA CGCAAGAAGC AGGTGGACTT TTCGGATAAA TATTACAACA CGCCGCCCGG CGTGGCCGCG CCCAAGGATA CCGACATCAA GGGCGTGACC AAGGAAGACC TTGCAGGCAA GACCATCGGC GTCCAGGTTT CCACAACCCA CGCGAACTAT GCCGAGCAGA CCTTCACCGA CAGCACGATC AAGGCTTATC CCACGGCGGA AGAATATCGC CTTGATCTTG CCAATGGTCG TCTCGATGCG GTCAATGACG ACAGCGTGAC CTTGTCGGAA TGGCTGAAGA CGCCGGATGG CGCCTGCTGC AAGACGGTCG GCAACCTACC CGCCGGACGA TGCAATCCAT GGTGTCGGCG TTGGCGTTGC CTTCAAGAAG GGCCGCCCGG AACTGGTCGA AAAGTTCAAC GCTGCCATCA AGGCTATTCG CAAGAATGGC AAGTA
Details for BrsuA.17285.b.B2.GE36803
HARVESTED ON: 3/27/2013
SEQUENCED ON: 3/30/2013
EXPECTED MW: 25kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA EEPLKLVIGT EGAYPPFNFI NPDGSLSGFD VDIARALCDE MKAECTFITQ EWDGTIPALQ AGKFDAYVSS MSITEERKKQ VDFSDKYYNT PPGVAAPKDT DIKGVTKEDL AGKTIGVQVS TTHANYAEQT FTDSTIKAYP TAEEYRLDLA NGRLDAVNDD SVTLSEWLKT PDGACCKTVG NLPAGRCNPW CRRWRCLQEG PPGTGRKVQR CHQGYSQEWQ V
Validated NT Sequence
ttgnnnnntt taagaaggag anataccatg gctcaccacc accaccacca tatggctgaa gagcccctca agctcgtcat cggaaccgag ggggcatatc cgccatttaa tttcatcaat cctgatggct cgctatcggg cttcgatgtg gatattgctc gggcgctttg cgatgagatg aaggcggagt gcacattcat tacgcaggaa tgggatggca ccatcccggc attgcaggca ggcaagttcg acgcctatgt gtcttccatg tcgattacgg aagaacgcaa gaagcaggtg gacttttcgg ataaatatta caacacgccg cccggcgtgg ccgcgcccaa ggataccgac atcaagggcg tgaccaagga agaccttgca ggcaagacca tcggcgtcca ggtttccaca acccacgcga actatgccga gcagaccttc accgacagca cgatcaaggc ttatcccacg gcggaagaat atcgccttga tcttgccaat ggtcgtctcg atgcggtcaa tgacgacagc gtgaccttgt cggaatggct gaagacgccg gatggcgcct gctgcaagac ggtcggcaac ctacccgccg gacgatgcaa tccatggtgt cggcgttggc gttgccttca agaagggccg cccggaactg gtcgaaaagt tcaacgctgc catcaaggct attcgcaaga atggcaagta taagtgagta agataggatc cggctgctac anacccccnn aaannnnnnn nnnnnccncn nnnncntnan cnnnggtncc ngcnncannn nacnnnncnn nnttnnnngn nnnnnnnnnn nnnnnnncnn cnnngcgtnt cgnngnnnnn ncnnnncncn nnctccnnnn nnnnccnnnc nnnnnnnnnn nnnnnnnnnn nntnnnnnnn ccnncccnnn ntctnnncgn ncnnnnnnnn natnccnnnn nnnnnncnnn nncctntnnn ncnnnnnnnn nnnnnncnnc nnnnnnnacn nnncnnnccn nnnnnnnccc ttnnctnnnc cnnncnnnnn naannnccgn nncnccccnc nnnnnnnntn cnnnnncngn nnctnncccc ccnnnnnnnn nnttnnnc
Expected Protein Sequence
MAHHHHHHAE EPLKLVIGTE GAYPPFNFIN PDGSLSGFDV DIARALCDEM KAECTFITQE WDGTIPALQA GKFDAYVSSM SITEERKKQV DFSDKYYNTP PGVAAPKDTD IKGVTKEDLA GKTIGVQVST THANYAEQTF TDSTIKAYPT AEEYRLDLAN GRLDAVNDDS VTLSEWLKTP DGACCKTVGN LPAGRCNPWC RRWRCLQEGP PGTGRKVQRC HQGYSQEWQV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgctgaag agcccctcaa gctcgtcatc ggaaccgagg gggcatatcc gccatttaat ttcatcaatc ctgatggctc gctatcgggc ttcgatgtgg atattgctcg ggcgctttgc gatgagatga aggcggagtg cacattcatt acgcaggaat gggatggcac catcccggca ttgcaggcag gcaagttcga cgcctatgtg tcttccatgt cgattacgga agaacgcaag aagcaggtgg acttttcgga taaatattac aacacgccgc ccggcgtggc cgcgcccaag gataccgaca tcaagggcgt gaccaaggaa gaccttgcag gcaagaccat cggcgtccag gtttccacaa cccacgcgaa ctatgccgag cagaccttca ccgacagcac gatcaaggct tatcccacgg cggaagaata tcgccttgat cttgccaatg gtcgtctcga tgcggtcaat gacgacagcg tgaccttgtc ggaatggctg aagacgccgg atggcgcctg ctgcaagacg gtcggcaacc tacccgccgg acgatgcaat ccatggtgtc ggcgttggcg ttgccttcaa gaagggccgc ccggaactgg tcgaaaagtt caacgctgcc atcaaggcta ttcgcaagaa tggcaagtat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat