SSGCID: Target Page

BrsuA.17285.a

Amino acid ABC transporter, periplasmic amino acid-binding protein

CENTER ID:	BrsuA.17285.a
ORGANISM:	Brucella suis 1330
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuA.17285.a.A1.GE34010	full length		1	268

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|204722.5.peg.968
RefSeq:	NP_697965.1
UniProt:	A0A0H3G6N1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRVLKRIAMA TSVAALAITV GSMAAGVANA EEPLKLTIGT EGAYPPFNFI KPDGTLSGFD VDIARALCDQ MKAKCEFVTQ DWDGAIPALQ SGKFDAFIAS MSITDERKKQ VDFTNKYYNT PPGIAAPKDT DIKGVTKEDL AGKTIGVQGS TTHANYSEKT YTDSTIKSYP TAEEYRLDLA NGRLDAVNDD SVTLAEWLKS PDGACCKMVG TFPPVTEIHG PGAGIAIKKG RPELVAKFNA AIKAIRANGK YKEINDKYFD FDAYGSEN

NT Sequence

atgcgcgtat tgaagcgtat cgctatggcg acatcggttg cagccttggc aatcacggtt ggcagcatgg ctgcgggcgt cgccaatgca gaagaaccgc tcaagctcac aatcggcacc gaaggcgcct atcctccttt caatttcatc aagccggatg gcacgctatc gggcttcgac gttgacattg cccgcgcgct ctgtgaccaa atgaaagcca agtgcgagtt cgtgacccag gactgggatg gcgcaattcc agcacttcag tccggcaagt tcgatgcctt catcgcctcc atgtccatca cggacgagcg caagaagcag gtggacttca ccaataaata ttacaatacg ccacccggca tcgccgctcc gaaggatacg gatatcaagg gcgtaaccaa ggaagacctt gcaggcaaga ccatcggtgt gcagggttcc acgacgcacg ccaactattc ggaaaagacc tataccgaca gcaccatcaa gtcctatccg acagcggaag aatatcgcct tgatcttgcc aatggccgcc ttgatgccgt caatgatgac agtgttacgc ttgctgaatg gctgaagtca ccggatggcg cctgctgcaa gatggtcggc accttcccgc ccgtgacgga aattcatggc ccgggcgcag gcatcgccat caagaagggc cgcccggaac ttgtcgcgaa attcaacgcc gcgatcaagg caatccgggc caacggcaag tataaggaaa tcaacgataa atatttcgac ttcgatgctt acggttccga aaactga

Details for BrsuA.17285.a.A1.GE34010

HARVESTED ON:	11/21/2011
SEQUENCED ON:	11/29/2011
EXPECTED MW:	31kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	91
PERCENT COVERAGE:	111

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMRVLKRIAM ATSVAALAIT VGSMAAGVAN AEEPLKLTIG TEGAYPPFNF IKPDGTLSGF DVDIARALCD QMKAKCEFVT QDWDGAIPAL QSGKFDAFIA SMSITDERKK QVDFTNKYYN TPPGIAAPKD TDIKGVTKED LAGKTIGVQG STTHANYSEK TYTDSTIKSY PTAEEYRLDL ANGRLDAVXD DXVTLAEWLK SPDGAXCKXV GTFPPVTEFM ARGAXXAXKX GRPELVAKFN AAIKAIRANG XYKEINDNIS TSMHGXXXNK QHEQXXQPSF SRXXXXXKPE XXLXXXXXXX XK

Validated NT Sequence

atttnnnnnn ntttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcgcg tattgaagcg tatcgctatg gcgacatcgg ttgcagcctt ggcaatcacg gttggcagca tggctgcggg cgtcgccaat gcagaagaac cgctcaagct cacaatcggc accgaaggcg cctatcctcc tttcaatttc atcaagccgg atggcacgct atcgggcttc gacgttgaca ttgcccgcgc gctctgtgac caaatgaaag ccaagtgcga gttcgtgacc caggactggg atggcgcaat tccagcactt cagtccggca agttcgatgc cttcatcgcc tccatgtcca tcacggacga gcgcaagaag caggtggact tcaccaataa atattacaat acgccacccg gcatcgccgc tccgaaggat acggatatca agggcgtaac caaggaagac cttgcaggca agaccatcgg tgtgcagggt tccacgacgc acgccaacta ttcggaaaag acctataccg acagcaccat caagtcctat ccgacagcgg aagaatatcg ccttgatctt gccaatggcc gccttgatgc cgtcnatgat gacngtgtta cgcttgctga atggctgaag tcaccggatg gcgccngctg caaganggtc ggcaccttcc cgcccgtgac ggaattcatg gcccggggcg cangcntcgc cntcaagaan ggccgcccgg aacttgtcgc gaaattcaac gccgcgatca aggcaatccg ggccaacggc nagtataagg aaatcaacga taatatttcg acntcnatgc acggttnnna aantaataaa cagcacgaac aanttcngca gccnagcttc tcgaggancn nncngcnanc aaagcccgaa angaanctna nttngncnnn nnnnnnnnnn nnaaaaa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMRVLKRIAM ATSVAALAIT VGSMAAGVAN AEEPLKLTIG TEGAYPPFNF IKPDGTLSGF DVDIARALCD QMKAKCEFVT QDWDGAIPAL QSGKFDAFIA SMSITDERKK QVDFTNKYYN TPPGIAAPKD TDIKGVTKED LAGKTIGVQG STTHANYSEK TYTDSTIKSY PTAEEYRLDL ANGRLDAVND DSVTLAEWLK SPDGACCKMV GTFPPVTEIH GPGAGIAIKK GRPELVAKFN AAIKAIRANG KYKEINDKYF DFDAYGSEN

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgcgtattg aagcgtatcg ctatggcgac atcggttgca gccttggcaa tcacggttgg cagcatggct gcgggcgtcg ccaatgcaga agaaccgctc aagctcacaa tcggcaccga aggcgcctat cctcctttca atttcatcaa gccggatggc acgctatcgg gcttcgacgt tgacattgcc cgcgcgctct gtgaccaaat gaaagccaag tgcgagttcg tgacccagga ctgggatggc gcaattccag cacttcagtc cggcaagttc gatgccttca tcgcctccat gtccatcacg gacgagcgca agaagcaggt ggacttcacc aataaatatt acaatacgcc acccggcatc gccgctccga aggatacgga tatcaagggc gtaaccaagg aagaccttgc aggcaagacc atcggtgtgc agggttccac gacgcacgcc aactattcgg aaaagaccta taccgacagc accatcaagt cctatccgac agcggaagaa tatcgccttg atcttgccaa tggccgcctt gatgccgtca atgatgacag tgttacgctt gctgaatggc tgaagtcacc ggatggcgcc tgctgcaaga tggtcggcac cttcccgccc gtgacggaaa ttcatggccc gggcgcaggc atcgccatca agaagggccg cccggaactt gtcgcgaaat tcaacgccgc gatcaaggca atccgggcca acggcaagta taaggaaatc aacgataaat atttcgactt cgatgcttac ggttccgaaa actaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa