SSGCID: Target Page

BrsuA.17194.a

BspD VirB type IV secretion effector protein

CENTER ID:	BrsuA.17194.a
ORGANISM:	Brucella suis 1330
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuA.17194.a.B2.GE38173	soluble domain		27	311

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuA.17194.a.B2.PW37625	soluble domain		27	311

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|204722.5.peg.1622
RefSeq:	NP_698585.1
RefSeq:	YP_005616408.1
UniProt:	A0A0H3G412

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIQSALFLML GVLVAVFVVV LLGPSVWRRA FFLARRQVQA ELPITLAEIR ADRDGLRAEH AVAVSKLEQL LKLERGKTAE QKVTLARQQD ELKRIPLLEQ NIARLEKRLG EEEKGAAEAR QARDTALEKA EILQAELERV QGHYVALESL ADTLRIEISA HEAEISRLMS EITEMRHDRK DATARYNELS TQLTAAQTKL KSETRRNGEL QQKLEKLISE LSDAQEKLER QNRHGDGALM SADQIEIARQ NAALREEMAT LAARMVAITA EREGPDSPIH KLLKGTALTE KGKGKKNKAP KSLATGIRDM Q

NT Sequence

gtgattcaat ccgctctctt tttgatgctt ggcgttctcg tggcggtttt cgtcgtggtt ctgcttggtc cttccgtatg gcggcgcgcc ttctttctgg cgcggcgtca ggtgcaggcc gaactgccga taaccttagc tgaaatccgt gccgaccgag acggccttcg cgccgagcac gctgtcgccg tgagcaagct tgaacaattg ctgaagctgg aacgcggcaa gacggcggaa caaaaagtga cattggcgcg ccagcaggat gagctgaaac gcattcccct tcttgaacaa aacattgcca ggcttgaaaa gcggcttggt gaggaggaaa agggagccgc tgaagcccgg caggcgcgcg atacggcgct tgaaaaagcg gaaattctcc aggccgaact ggagcgggtg cagggccatt atgttgcact tgagagcctt gcggatacgc tgcgcatcga aataagcgcg catgaggccg agattagtcg tttaatgagc gagattaccg aaatgcgcca tgaccgaaag gatgcaactg cccgctataa cgagctttca acgcagttga cggcggcgca gacgaaactg aaaagcgaga cgcgccgcaa tggtgaattg cagcagaagc tggaaaagct gatttccgaa ctttccgatg cgcaggaaaa actggagcgg caaaatcgtc atggcgatgg cgcgctcatg agtgccgatc agattgagat tgcgcgccaa aatgctgcgc tgcgtgagga aatggccacc cttgccgccc gcatggttgc cattacggcc gaaagggaag gacccgattc accgatccat aagctgctca agggaacggc cctgacggaa aagggcaaag gcaaaaagaa taaagcgccc aaaagcctcg caaccggcat ccgcgacatg caatag

Details for BrsuA.17194.a.B2.GE38173

HARVESTED ON:	6/11/2014
SEQUENCED ON:	6/12/2014
EXPECTED MW:	33kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMW RRAFFLARRQ VQAELPITLA EIRADRDGLR AEHAVAVSKL EQLLKLERGK TAEQKVTLAR QQDELKRIPL LEQNIARLEK RLGEEEKGAA EARQARDTAL EKAEILQAEL ERVQGHYVAL ESLADTLRIE ISAHEAEISR LMSEITEMRH DRKDATARYN ELSTQLTAAQ TKLKSETRRN GELQQKLEKL ISELSDAQEK LERQNRHGDG ALMSADQIEI ARQNAALREE MATLAARMVA ITAEREGPDS PIHKLLKGTA LTEKGKGKKN KAPKSLATGI RDMQ

Validated NT Sequence

atggctcacc accaccacca ccatatgtgg cggcgcgcct tctttctggc gcggcgtcag gtgcaggccg aactgccgat aaccttagct gaaatccgtg ccgaccgaga cggccttcgc gccgagcacg ctgtcgccgt gagcaagctt gaacaattgc tgaagctgga acgcggcaag acggcggaac aaaaagtgac attggcgcgc cagcaggatg agctgaaacg cattcccctt cttgaacaaa acattgccag gcttgaaaag cggcttggtg aggaggaaaa gggagccgct gaagcccggc aggcgcgcga tacggcgctt gaaaaagcgg aaattctcca ggccgaactg gagcgggtgc agggccatta tgttgcactt gagagccttg cggatacgct gcgcatcgaa ataagcgcgc atgaggccga gattagtcgt ttaatgagcg agattaccga aatgcgccat gaccgaaagg atgcaactgc ccgctataac gagctttcaa cgcagttgac ggcggcgcag acgaaactga aaagcgagac gcgccgcaat ggtgaattgc agcagaagct ggaaaagctg atttccgaac tttccgatgc gcaggaaaaa ctggagcggc aaaatcgtca tggcgatggc gcgctcatga gtgccgatca gattgagatt gcgcgccaaa atgctgcgct gcgtgaggaa atggccaccc ttgccgcccg catggttgcc attacggccg aaagggaagg acccgattca ccgatccata agctgctcaa gggaacggcc ctgacggaaa agggcaaagg caaaaagaat aaagcgccca aaagcctcgc aaccggcatc cgcgacatgc aataagtgag taagat

Expected Protein Sequence

MAHHHHHHWR RAFFLARRQV QAELPITLAE IRADRDGLRA EHAVAVSKLE QLLKLERGKT AEQKVTLARQ QDELKRIPLL EQNIARLEKR LGEEEKGAAE ARQARDTALE KAEILQAELE RVQGHYVALE SLADTLRIEI SAHEAEISRL MSEITEMRHD RKDATARYNE LSTQLTAAQT KLKSETRRNG ELQQKLEKLI SELSDAQEKL ERQNRHGDGA LMSADQIEIA RQNAALREEM ATLAARMVAI TAEREGPDSP IHKLLKGTAL TEKGKGKKNK APKSLATGIR DMQ

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattggcggc gcgccttctt tctggcgcgg cgtcaggtgc aggccgaact gccgataacc ttagctgaaa tccgtgccga ccgagacggc cttcgcgccg agcacgctgt cgccgtgagc aagcttgaac aattgctgaa gctggaacgc ggcaagacgg cggaacaaaa agtgacattg gcgcgccagc aggatgagct gaaacgcatt ccccttcttg aacaaaacat tgccaggctt gaaaagcggc ttggtgagga ggaaaaggga gccgctgaag cccggcaggc gcgcgatacg gcgcttgaaa aagcggaaat tctccaggcc gaactggagc gggtgcaggg ccattatgtt gcacttgaga gccttgcgga tacgctgcgc atcgaaataa gcgcgcatga ggccgagatt agtcgtttaa tgagcgagat taccgaaatg cgccatgacc gaaaggatgc aactgcccgc tataacgagc tttcaacgca gttgacggcg gcgcagacga aactgaaaag cgagacgcgc cgcaatggtg aattgcagca gaagctggaa aagctgattt ccgaactttc cgatgcgcag gaaaaactgg agcggcaaaa tcgtcatggc gatggcgcgc tcatgagtgc cgatcagatt gagattgcgc gccaaaatgc tgcgctgcgt gaggaaatgg ccacccttgc cgcccgcatg gttgccatta cggccgaaag ggaaggaccc gattcaccga tccataagct gctcaaggga acggccctga cggaaaaggg caaaggcaaa aagaataaag cgcccaaaag cctcgcaacc ggcatccgcg acatgcaatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat

Details for BrsuA.17194.a.B2.PW37625

PURIFICATION DATe:	1/6/2015
CONCENTRATION:	9mg/ml
OBSERVED MW:	35kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	1
VIAL VOLUME:	110µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTGGCGGC GCGCCTTCTT TCTGGCGCGG CGTCAGGTGC AGGCCGAACT GCCGATAACC TTAGCTGAAA TCCGTGCCGA CCGAGACGGC CTTCGCGCCG AGCACGCTGT CGCCGTGAGC AAGCTTGAAC AATTGCTGAA GCTGGAACGC GGCAAGACGG CGGAACAAAA AGTGACATTG GCGCGCCAGC AGGATGAGCT GAAACGCATT CCCCTTCTTG AACAAAACAT TGCCAGGCTT GAAAAGCGGC TTGGTGAGGA GGAAAAGGGA GCCGCTGAAG CCCGGCAGGC GCGCGATACG GCGCTTGAAA AAGCGGAAAT TCTCCAGGCC GAACTGGAGC GGGTGCAGGG CCATTATGTT GCACTTGAGA GCCTTGCGGA TACGCTGCGC ATCGAAATAA GCGCGCATGA GGCCGAGATT AGTCGTTTAA TGAGCGAGAT TACCGAAATG CGCCATGACC GAAAGGATGC AACTGCCCGC TATAACGAGC TTTCAACGCA GTTGACGGCG GCGCAGACGA AACTGAAAAG CGAGACGCGC CGCAATGGTG AATTGCAGCA GAAGCTGGAA AAGCTGATTT CCGAACTTTC CGATGCGCAG GAAAAACTGG AGCGGCAAAA TCGTCATGGC GATGGCGCGC TCATGAGTGC CGATCAGATT GAGATTGCGC GCCAAAATGC TGCGCTGCGT GAGGAAATGG CCACCCTTGC CGCCCGCATG GTTGCCATTA CGGCCGAAAG GGAAGGACCC GATTCACCGA TCCATAAGCT GCTCAAGGGA ACGGCCCTGA CGGAAAAGGG CAAAGGCAAA AAGAATAAAG CGCCCAAAAG CCTCGCAACC GGCATCCGCG ACATGCAATG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT