SSGCID: Target Page

BrsuA.11416.a

Chromosome (plasmid) partitioning protein ParA / Sporulation initiation inhibitor protein Soj

CENTER ID:	BrsuA.11416.a
ORGANISM:	Brucella suis 1330
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuA.11416.a.B1.GE36801	Full length( BrsuA.11416.a )		1	265

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruSui107850_2093
BV-BRC:	fig\|204722.5.peg.2093
RefSeq:	NP_699034.1
RefSeq:	YP_005616858.1
UniProt:	A0A0H3GDQ5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNKMSRIITI ANQKGGVGKT TTAINLATAL AAIGETVLIV DLDPQGNAST GLGIDRHNRP LSSYDVLTQA SSVPEAAMQT DVPNLFIVPS TLDLLGIEME IAQSADRTRR LRDALRFDSG VSERFTYVLV DCPPSLNLLT LNAMAAADSV LVPLQCEFFA LEGLSQLLQT VDQVRSTINP ELSIQGIVLT MFDSRNNLAT QVVDDVRAFM GEKVYRTVIP RNVRVSEAPS HGKPAILYDL KCAGSQAYLQ LASEVIQRER QLQAA

NT Sequence

ATGAACAAGA TGTCCAGGAT CATAACCATT GCGAACCAGA AGGGTGGGGT GGGCAAGACC ACTACCGCCA TTAATCTGGC GACCGCGCTT GCTGCCATCG GCGAGACGGT GCTGATTGTC GATCTTGATC CGCAGGGCAA TGCCAGCACG GGTCTCGGCA TTGACCGGCA TAACCGCCCT TTGTCCTCCT ATGATGTGCT GACGCAGGCT TCGTCCGTAC CGGAAGCTGC GATGCAGACG GATGTGCCCA ACCTCTTTAT TGTGCCATCA ACGCTCGATC TTCTGGGCAT TGAAATGGAA ATCGCCCAAT CGGCAGATCG TACCCGGCGT TTGCGCGATG CATTGCGTTT CGATTCTGGT GTTTCGGAGC GATTTACTTA CGTTCTGGTC GATTGCCCGC CGTCCCTTAA TCTGCTGACA TTGAATGCAA TGGCCGCTGC CGACTCCGTT CTGGTGCCGC TGCAATGCGA ATTTTTCGCG CTTGAGGGGT TGAGCCAGCT CTTGCAAACT GTCGATCAGG TGCGTTCCAC CATCAATCCT GAACTGTCCA TACAGGGGAT TGTGTTGACA ATGTTTGACA GCCGCAACAA TCTGGCAACG CAAGTGGTAG ATGATGTGCG TGCTTTCATG GGCGAAAAGG TCTATCGCAC GGTTATTCCG CGCAATGTTC GTGTTTCGGA AGCACCGTCC CATGGAAAAC CAGCTATTCT CTACGATCTG AAATGCGCCG GTAGCCAGGC CTATCTGCAA CTCGCCTCGG AGGTAATCCA GCGCGAGCGG CAATTACAGG CTGCC

Details for BrsuA.11416.a.B1.GE36801

HARVESTED ON:	3/27/2013
SEQUENCED ON:	3/30/2013
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMN KMSRIITIAN QKGGVGKTTT AINLATALAA IGETVLIVDL DPQGNASTGL GIDRHNRPLS SYDVLTQASS VPEAAMQTDV PNLFIVPSTL DLLGIEMEIA QSADRTRRLR DALRFDSGVS ERFTYVLVDC PPSLNLLTLN AMAAADSVLV PLQCEFFALE GLSQLLQTVD QVRSTINPEL SIQGIVLTMF DSRNNLATQV VDDVRAFMGE KVYRTVIPRN VRVSEAPSHG KPAILYDLKC AGSQAYLQLA SEVIQRERQL QAA

Validated NT Sequence

atcttactca cttaggcagc ctgtaattgc cgctcgcgct ggattacctc cgaggcgagt tgcagatagg cctggctacc ggcgcatttc agatcgtaga gaatagctgg ttttccatgg gacggtgctt ccgaaacacg aacattgcgc ggaataaccg tgcgatagac cttttcgccc atgaaagcac gcacatcatc taccacttgc gttgccagat tgttgcggct gtcaaacatt gtcaacacaa tcccctgtat ggacagttca ggattgatgg tggaacgcac ctgatcgaca gtttgcaaga gctggctcaa cccctcaagc gcgaaaaatt cgcattgcag cggcaccaga acggagtcgg cagcggccat tgcattcaat gtcagcagat taagggacgg cgggcaatcg accagaacgt aagtaaatcg ctccgaaaca ccagaatcga aacgcaatgc atcgcgcaaa cgccgggtac gatctgccga ttgggcgatt tccatttcaa tgcccagaag atcgagcgtt gatggcacaa taaagaggtt gggcacatcc gtctgcatcg cagcttccgg tacggacgaa gcctgcgtca gcacatcata ggaggacaaa gggcggttat gccggtcaat gccgagaccc gtgctggcat tgccctgcgg atcaagatcg acaatcagca ccgtctcgcc gatggcagca agcgcggtcg ccagattaat ggcggtagtg gtcttgccca ccccaccctt ctggttcgca atggttatga tcctggacat cttgttcata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaca agatgtccag gatcataacc attgcgaacc agaagggtgg ggtgggcaag accactaccg ccattaatct ggcgaccgcg cttgctgcca tcggcgagac ggtgctgatt gtcgatcttg atccgcaggg caatgccagc acgggtctcg gcattgaccg gcataaccgc cctttgtcct cctatgatgt gctgacgcag gcttcgtccg taccggaagc tgcgatgcag acggatgtgc ccaacctctt tattgtgcca tcaacgctcg atcttctggg cattgaaatg gaaatcgccc aatcggcaga tcgtacccgg cgtttgcgcg atgcattgcg tttcgattct ggtgtttcgg agcgatttac ttacgttctg gtcgattgcc cgccgtccct taatctgctg acattgaatg caatggccgc tgccgactcc gttctggtgc cgctgcaatg cgaatttttc gcgcttgagg ggttgagcca gctcttgcaa actgtcgatc aggtgcgttc caccatcaat cctgaactgt ccatacaggg gattgtgttg acaatgtttg acagccgcaa caatctggca acgcaagtgg tagatgatgt gcgtgctttc atgggcgaaa aggtctatcg cacggttatt ccgcgcaatg ttcgtgtttc ggaagcaccg tcccatggaa aaccagctat tctctacgat ctgaaatgcg ccggtagcca ggcctatctg caactcgcct cggaggtaat ccagcgcgag cggcaattac aggctgcctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat