SSGCID: Target Page

BrsuA.10014.x

Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1)

CENTER ID:	BrsuA.10014.x
ORGANISM:	Brucella suis 1330
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuA.10014.x.B1.GE36799	Full length( BrsuA.10014.x )		1	242

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruSui107850_3428
BV-BRC:	fig\|204722.5.peg.3428
RefSeq:	NP_700349.1
RefSeq:	YP_005614856.1
UniProt:	A0A0H3GGH8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSPHDSNIIL DVRNLSAGYG ELPVIRDLNL HVKEAEIVAL VGSNGAGKTT FLRALSHLIH SEGQIEFCGE DVSRSTSDYV FRRGLVHIPE GRQLFDRMSV EENLLMGAYR SKDKARTKAD LEKVYVLFPR LAERRKQLAG SMSGGEQQMC AMARALMANP VLQLVDEMSL GLAPVVVDQL MTILHDIRKS EQVTVLLVEQ DIHLALDGAD RGYVMETGTF TLEGKAQDLI HDERMQKAYL GL

NT Sequence

ATGAGCCCCC ACGACAGCAA CATCATTCTC GATGTACGCA ACCTATCGGC TGGCTATGGT GAGCTGCCGG TCATTCGTGA TCTTAATCTT CATGTCAAGG AAGCGGAAAT CGTAGCACTG GTTGGCAGCA ACGGCGCTGG CAAGACAACG TTTCTGCGCG CGCTTTCTCA TCTGATCCAT TCCGAGGGCC AAATCGAGTT TTGTGGCGAA GACGTTTCCA GATCGACATC GGATTATGTA TTTCGACGCG GATTGGTACA TATCCCCGAA GGGCGGCAAC TCTTCGACCG TATGTCGGTG GAAGAAAACT TGTTGATGGG CGCTTATCGC AGCAAGGACA AGGCCCGGAC CAAGGCTGAT CTGGAAAAGG TCTATGTTCT ATTTCCCCGC CTCGCCGAAA GGCGCAAGCA GTTGGCTGGC TCCATGTCAG GCGGAGAGCA GCAGATGTGC GCCATGGCTC GGGCGCTGAT GGCCAATCCC GTTTTACAGC TGGTTGATGA AATGAGCCTC GGCCTTGCGC CCGTGGTTGT CGACCAGTTG ATGACGATCC TGCATGACAT TCGCAAGTCA GAGCAGGTAA CCGTGCTTCT GGTCGAGCAG GATATTCATC TTGCTCTGGA CGGCGCAGAC CGTGGATATG TGATGGAAAC CGGCACCTTC ACGCTGGAAG GCAAGGCGCA GGATCTTATT CACGATGAGA GGATGCAAAA AGCATATTTG GGTTTG

Details for BrsuA.10014.x.B1.GE36799

HARVESTED ON:	3/27/2013
SEQUENCED ON:	3/30/2013
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS PHDSNIILDV RNLSAGYGEL PVIRDLNLHV KEAEIVALVG SNGAGKTTFL RALSHLIHSE GQIEFCGEDV SRSTSDYVFR RGLVHIPEGR QLFDRMSVEE NLLMGAYRSK DKARTKADLE KVYVLFPRLA ERRKQLAGSM SGGEQQMCAM ARALMANPVL QLVDEMSLGL APVVVDQLMT ILHDIRKSEQ VTVLLVEQDI HLALDGADRG YVMETGTFTL EGKAXDLIHD ERXQKAYLGL

Validated NT Sequence

ttnnnnnnnt taagaaggag anataccatg gctcaccacc accaccacca tatgagcccc cacgacagca acatcattct cgatgtacgc aacctatcgg ctggctatgg tgagctgccg gtcattcgtg atcttaatct tcatgtcaag gaagcggaaa tcgtagcact ggttggcagc aacggcgctg gcaagacaac gtttctgcgc gcgctttctc atctgatcca ttccgagggc caaatcgagt tttgtggcga agacgtttcc agatcgacat cggattatgt atttcgacgc ggattggtac atatccccga agggcggcaa ctcttcgacc gtatgtcggt ggaagaaaac ttgttgatgg gcgcttatcg cagcaaggac aaggcccgga ccaaggctga tctggaaaag gtctatgttc tatttccccg cctcgccgaa aggcgcaagc agttggctgg ctccatgtca ggcggagagc agcagatgtg cgccatggct cgggcgctga tggccaatcc cgttttacag ctggttgatg aaatgagcct cggccttgcg cccgtggttg tcgaccagtt gatgacgatc ctgcatgaca ttcgcaagtc agagcaggta accgtgcttc tggtcgagca ggatattcat cttgctctgg acggcgcaga ccgtggatat gtgatggaaa ccggcacctt cacgctggaa ggcaaggcgc angatcttat tcacgatgag aggangcaaa aagcatattt gggtttgtaa gtgagtaana taggatccgg ctgnnnnnnn nnccccnaan nnnnnnnnnn nnnnnaannc nnncnnngnc gnnnnnnnnn nnnncnnnnn nnnncnnnnn cnannnnnnn nnncnnnnnn tgncccnnnn ncnncntnnn nncnccnnnn nnncnggnnn ncnnnannna nnnnnncccn nnnggnnnnn nnntntnnnn nnnnnnnnng nccnnnnnnn ntnncnnnnn nnnnccnnnn nnnnncnnnn nnnnnnnnnn ntntcnnctn nnnnnntnnn cnccnnnnnn nnnnnnnnnn nnnncnnnnt nnnnnntnnn ncnntnnnnn nnncnccccc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcc cccacgacag caacatcatt ctcgatgtac gcaacctatc ggctggctat ggtgagctgc cggtcattcg tgatcttaat cttcatgtca aggaagcgga aatcgtagca ctggttggca gcaacggcgc tggcaagaca acgtttctgc gcgcgctttc tcatctgatc cattccgagg gccaaatcga gttttgtggc gaagacgttt ccagatcgac atcggattat gtatttcgac gcggattggt acatatcccc gaagggcggc aactcttcga ccgtatgtcg gtggaagaaa acttgttgat gggcgcttat cgcagcaagg acaaggcccg gaccaaggct gatctggaaa aggtctatgt tctatttccc cgcctcgccg aaaggcgcaa gcagttggct ggctccatgt caggcggaga gcagcagatg tgcgccatgg ctcgggcgct gatggccaat cccgttttac agctggttga tgaaatgagc ctcggccttg cgcccgtggt tgtcgaccag ttgatgacga tcctgcatga cattcgcaag tcagagcagg taaccgtgct tctggtcgag caggatattc atcttgctct ggacggcgca gaccgtggat atgtgatgga aaccggcacc ttcacgctgg aaggcaaggc gcaggatctt attcacgatg agaggatgca aaaagcatat ttgggtttgt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat