BrsuA.10014.w

Oligopeptide transport ATP-binding protein OppF (TC 3.A.1.5.1)

CENTER ID: BrsuA.10014.w
ORGANISM: Brucella suis 1330
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuA.10014.w.B1.GE36798 Full length( BrsuA.10014.w ) 1 334
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruSui107850_3302
BV-BRC: fig|204722.5.peg.3302
RefSeq: NP_700240.1
RefSeq: YP_005614748.1
UniProt: A0A0H3G8D3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRENSTEIAL SVRGLRKSFP VKGGTFIQRD IAAVKAVDDI SFDLRKGETL GLVGESGCGK STLGRCILRL IEPSDGQVLF RGNDMTKLNA RDMRAARKHL QFVFQDPIAS LHPRMRIKES IAEPLRISDL TQAERKERVQ EMLRLVRFNP EHGKRYPHEL SGGQRRRVVI ACALALNPQV MVLDEPVSAL DVSVQAGVLN LLQDIQERFG TSYVFIAHDL SVVRHISHTV AVMYLGRIVE KAPEARLYGH PLHPYTQALM SAVPLADPVI ERKRQQILLK GDVPSPINPP SGCPFRTRCF RAQAVCAEVR PELQSRGGGH EVACHFPEEW RAAS
NT Sequence
ATGCGTGAGA ACTCCACGGA GATCGCGCTT TCGGTTCGCG GGCTGCGAAA GAGCTTTCCC GTCAAGGGCG GCACGTTCAT TCAGCGCGAT ATTGCCGCGG TCAAAGCCGT CGATGATATC TCGTTCGATC TGCGCAAAGG GGAAACGCTC GGCCTCGTCG GCGAGTCCGG CTGCGGAAAG TCCACGCTGG GACGATGTAT CCTTCGGCTG ATCGAACCGT CGGACGGGCA GGTCCTGTTC CGGGGGAACG ATATGACCAA ACTCAATGCG CGCGACATGC GCGCCGCGCG CAAGCATCTC CAGTTCGTGT TTCAGGACCC CATCGCCTCG CTTCATCCGC GCATGCGCAT CAAGGAAAGC ATTGCGGAGC CGTTACGCAT CAGCGATCTC ACGCAAGCAG AGCGCAAGGA GCGCGTGCAG GAAATGCTGC GTCTGGTGCG GTTCAACCCG GAGCACGGCA AACGCTATCC GCATGAGCTT TCCGGCGGGC AGCGTCGGCG CGTAGTGATT GCGTGTGCCT TGGCCCTCAA TCCGCAGGTC ATGGTGCTGG ATGAGCCCGT CTCGGCGCTC GATGTTTCCG TTCAGGCCGG TGTCTTGAAT CTCCTTCAGG ATATTCAGGA GCGGTTCGGG ACATCTTATG TTTTCATTGC CCATGACCTG TCGGTTGTGC GCCATATCTC GCACACCGTT GCCGTCATGT ATCTTGGCCG CATCGTGGAA AAGGCCCCCG AAGCCCGGCT TTATGGGCAT CCGCTGCATC CCTACACGCA GGCCTTGATG TCGGCAGTGC CTCTCGCCGA TCCGGTCATC GAGCGCAAGC GGCAGCAGAT ATTGCTGAAA GGCGACGTGC CAAGCCCGAT CAATCCGCCT TCCGGCTGTC CGTTCCGCAC CCGTTGTTTC AGGGCACAGG CAGTCTGCGC CGAAGTGAGG CCCGAATTGC AGAGCCGGGG AGGCGGCCAT GAGGTGGCGT GCCACTTCCC GGAGGAATGG CGGGCAGCAT CC
Details for BrsuA.10014.w.B1.GE36798
HARVESTED ON: 3/27/2013
SEQUENCED ON: 3/30/2013
EXPECTED MW: 38kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 96
PERCENT COVERAGE: 92
Validated AA Sequence
MAHHHHHHMR ENSTEIALSV RGLRKSFPVK GGTFIQRDIA AVKAVDDISF DLRKGETLGL VGESGCGKST LGRCILRLIE PSDGQVLFRG NDMTKLNARD MRAARKHLQF VFQDPIASLH PRMRIKESIA EPLRISDLTQ AERKERVQEM LRLVRFNPEH GKRYPHELSG GQRRRVVIAC ALALNPQVMV LDEPVSALDV SVQAGVLNLL QDIQERFGTS YVFIAHDLSV VRHISHTVAV MYLGRIVEKX PEARLYGHPL HPYTQALMSA VPLADPVIER KRQQILLKGD VPSPINPPSG CPFXTRCXXX XQSAPX
Validated NT Sequence
attttgttta ctttaagaag gaganatacc atggctcacc accaccacca ccatatgcgt gagaactcca cggagatcgc gctttcggtt cgcgggctgc gaaagagctt tcccgtcaag ggcggcacgt tcattcagcg cgatattgcc gcggtcaaag ccgtcgatga tatctcgttc gatctgcgca aaggggaaac gctcggcctc gtcggcgagt ccggctgcgg aaagtccacg ctgggacgat gtatccttcg gctgatcgaa ccgtcggacg ggcaggtcct gttccggggg aacgatatga ccaaactcaa tgcgcgcgac atgcgcgccg cgcgcaagca tctccagttc gtgtttcagg accccatcgc ctcgcttcat ccgcgcatgc gcatcaagga aagcattgcg gagccgttac gcatcagcga tctcacgcaa gcagagcgca aggagcgcgt gcaggaaatg ctgcgtctgg tgcggttcaa cccggagcac ggcaaacgct atccgcatga gctttccggc gggcagcgtc ggcgcgtagt gattgcgtgt gccttggccc tcaatccgca ggtcatggtg ctggatgagc ccgtctcggc gctcgatgtt tccgttcagg ccggtgtctt gaatctcctt caggatattc aggagcggtt cgggacatct tatgttttca ttgcccatga cctgtcggtt gtgcgccata tctcgcacac cgttgccgtc atgtatcttg gccgcatcgt ggaaaaggnc cccgaagccc ggctttatgg gcatccgctg catccctaca cgcaggcctt gatgtcggca gtgcctctcg ccgatccggt catcgagcgc aagcggcagc agatattgct gaaaggcgac gtgccaagcc cgatcaatcc gccttccggc tgtccgttcc ncacccgttg ttnnnnnnac nngcagtctg cgccnaantg angnccgaan tgcnnannnn ggnaggcngn catgnnnnnn gnnnnnnnnc ttnnnnnatg nnngnancnt nnnnnnnnnt annnnnnnnn nnntnnnnnn nnnnc
Expected Protein Sequence
MAHHHHHHMR ENSTEIALSV RGLRKSFPVK GGTFIQRDIA AVKAVDDISF DLRKGETLGL VGESGCGKST LGRCILRLIE PSDGQVLFRG NDMTKLNARD MRAARKHLQF VFQDPIASLH PRMRIKESIA EPLRISDLTQ AERKERVQEM LRLVRFNPEH GKRYPHELSG GQRRRVVIAC ALALNPQVMV LDEPVSALDV SVQAGVLNLL QDIQERFGTS YVFIAHDLSV VRHISHTVAV MYLGRIVEKA PEARLYGHPL HPYTQALMSA VPLADPVIER KRQQILLKGD VPSPINPPSG CPFRTRCFRA QAVCAEVRPE LQSRGGGHEV ACHFPEEWRA AS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgtg agaactccac ggagatcgcg ctttcggttc gcgggctgcg aaagagcttt cccgtcaagg gcggcacgtt cattcagcgc gatattgccg cggtcaaagc cgtcgatgat atctcgttcg atctgcgcaa aggggaaacg ctcggcctcg tcggcgagtc cggctgcgga aagtccacgc tgggacgatg tatccttcgg ctgatcgaac cgtcggacgg gcaggtcctg ttccggggga acgatatgac caaactcaat gcgcgcgaca tgcgcgccgc gcgcaagcat ctccagttcg tgtttcagga ccccatcgcc tcgcttcatc cgcgcatgcg catcaaggaa agcattgcgg agccgttacg catcagcgat ctcacgcaag cagagcgcaa ggagcgcgtg caggaaatgc tgcgtctggt gcggttcaac ccggagcacg gcaaacgcta tccgcatgag ctttccggcg ggcagcgtcg gcgcgtagtg attgcgtgtg ccttggccct caatccgcag gtcatggtgc tggatgagcc cgtctcggcg ctcgatgttt ccgttcaggc cggtgtcttg aatctccttc aggatattca ggagcggttc gggacatctt atgttttcat tgcccatgac ctgtcggttg tgcgccatat ctcgcacacc gttgccgtca tgtatcttgg ccgcatcgtg gaaaaggccc ccgaagcccg gctttatggg catccgctgc atccctacac gcaggccttg atgtcggcag tgcctctcgc cgatccggtc atcgagcgca agcggcagca gatattgctg aaaggcgacg tgccaagccc gatcaatccg ccttccggct gtccgttccg cacccgttgt ttcagggcac aggcagtctg cgccgaagtg aggcccgaat tgcagagccg gggaggcggc catgaggtgg cgtgccactt cccggaggaa tggcgggcag catcctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat