BrsuA.10014.u

Nickel transport ATP-binding protein NikD (TC 3.A.1.5.3)

CENTER ID: BrsuA.10014.u
ORGANISM: Brucella suis 1330
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuA.10014.u.B1.GE36796 Full length( BrsuA.10014.u ) 1 262
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruSui107850_3017
BV-BRC: fig|204722.5.peg.3017
RefSeq: NP_699974.1
RefSeq: YP_005614481.1
UniProt: Q8FVM9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTRKTLAIEG LTATTVIDGQ QRVLVDNLSL GVQRGRILAL VGASGSGKSM TCSAALGVLP PGVTASRGRV TIDGVPYAAN ALRGRHVATI MQNPRGAFNP VRTMRDHAIE TLQALGKLSS NPQDQIVHCM RAAGLEDVKT ILSLHPFEMS GGMLQRMMIA LALLSEAPFL FADEPTTDLD LVVQLRVLEL LEKLVEERDL GILLVTHDMG VVARLAHDVA VLDHGRLIEQ APVMDIFQTP GHEVTRMLVS AHLSLYGMEL NA
NT Sequence
TTGACCAGGA AGACACTCGC CATTGAAGGC CTGACCGCGA CCACCGTAAT AGACGGGCAG CAGCGCGTCC TTGTCGATAA TCTTTCTCTT GGCGTTCAAC GCGGACGCAT TCTCGCACTG GTCGGAGCAA GCGGTTCCGG CAAGTCGATG ACCTGTTCGG CAGCACTTGG TGTCCTGCCG CCCGGCGTCA CGGCCTCGCG CGGGCGTGTG ACGATAGATG GGGTTCCTTA TGCGGCAAAC GCGCTGCGCG GGCGGCATGT CGCGACCATC ATGCAAAACC CGCGCGGCGC CTTTAATCCT GTTCGCACCA TGCGCGACCA TGCCATCGAG ACCCTTCAGG CGCTTGGCAA GCTCAGTTCA AATCCGCAGG ATCAGATAGT GCATTGTATG CGGGCGGCCG GGCTGGAAGA CGTTAAAACC ATCCTCTCCT TACATCCGTT CGAGATGAGC GGCGGCATGT TGCAGCGGAT GATGATTGCG CTGGCGCTGC TCAGTGAGGC GCCATTCCTG TTTGCCGATG AGCCGACCAC CGATCTTGAT CTCGTCGTCC AGCTTCGGGT TCTGGAGCTT CTGGAAAAAC TGGTCGAGGA ACGCGACCTC GGCATTCTTC TTGTAACCCA TGATATGGGC GTCGTCGCTA GACTGGCCCA TGATGTCGCC GTCCTCGATC ATGGCCGCCT TATCGAACAG GCCCCGGTGA TGGATATCTT TCAAACGCCC GGACATGAGG TCACACGCAT GCTGGTCAGC GCGCATCTGT CGCTCTACGG TATGGAGCTC AATGCA
Details for BrsuA.10014.u.B1.GE36796
HARVESTED ON: 3/27/2013
SEQUENCED ON: 3/30/2013
EXPECTED MW: 29kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML TRKTLAIEGL TATTVIDGQQ RVLVDNLSLG VQRGRILALV GASGSGKSMT CSAALGVLPP GVTASRGRVT IDGVPYAANA LRGRHVATIM QNPRGAFNPV RTMRDHAIET LQALGKLSSN PQDQIVHCMR AAGLEDVKTI LSLHPFEMSG GMLQRMMIAL ALLSEAPFLF ADEPTTDLDL VVQLRVLELL EKLVEERDLG ILLVTHDMGV VARLAHDVAV LDHGRLIEQA PVMDIFQTPG HEVTRMLVSA HLSLYGMELN A
Validated NT Sequence
ttnnttactt taagaaggag anataccatg gctcaccacc accaccacca tatgttgacc aggaagacac tcgccattga aggcctgacc gcgaccaccg taatagacgg gcagcagcgc gtccttgtcg ataatctttc tcttggcgtt caacgcggac gcattctcgc actggtcgga gcaagcggtt ccggcaagtc gatgacctgt tcggcagcac ttggtgtcct gccgcccggc gtcacggcct cgcgcgggcg tgtgacgata gatggggttc cttatgcggc aaacgcgctg cgcgggcggc atgtcgcgac catcatgcaa aacccgcgcg gcgcctttaa tcctgttcgc accatgcgcg accatgccat cgagaccctt caggcgcttg gcaagctcag ttcaaatccg caggatcaga tagtgcattg tatgcgggcg gccgggctgg aagacgttaa aaccatcctc tccttacatc cgttcgagat gagcggcggc atgttgcagc ggatgatgat tgcgctggcg ctgctcagtg aggcgccatt cctgtttgcc gatgagccga ccaccgatct tgatctcgtc gtccagcttc gggttctgga gcttctggaa aaactggtcg aggaacgcga cctcggcatt cttcttgtaa cccatgatat gggcgtcgtc gctagactgg cccatgatgt cgccgtcctc gatcatggcc gccttatcga acaggccccg gtgatggata tctttcaaac gcccggacat gaggtcacac gcatgctggt cagcgcgcat ctgtcgctct acggtatgga gctcaatgca taagtgagta ananagnanc cnnnnnnnnn nnncccnnnn nnnnaaannn nnnnnnnnnn nnnnnnnntn ngnncncnnn nnncnnnnnn nnnncgntnn nnnnnnnnnn cnnnnnnnnn nngcgacttn ctncccnnnt nnnnnnnnan anannnnnnn nnnnnnnnnn nnncccnnnn nnnnnnnnnn nctcctcntc nncnnnnnnn nnnnnccnnc nnnccnnnnn nnncnnncnc cnnnnnnnnn nnnnntnnnc nannnnnnnn nnnnnnnnnc nnnnnnnnnn nnnnnnnnnc ncccncnctn nnnnnnntnn nnnnnnnnnn nnnnnnnnnn cnnnnnnccc cnncnnnnnn nnnnnnnncc
Expected Protein Sequence
MAHHHHHHMT RKTLAIEGLT ATTVIDGQQR VLVDNLSLGV QRGRILALVG ASGSGKSMTC SAALGVLPPG VTASRGRVTI DGVPYAANAL RGRHVATIMQ NPRGAFNPVR TMRDHAIETL QALGKLSSNP QDQIVHCMRA AGLEDVKTIL SLHPFEMSGG MLQRMMIALA LLSEAPFLFA DEPTTDLDLV VQLRVLELLE KLVEERDLGI LLVTHDMGVV ARLAHDVAVL DHGRLIEQAP VMDIFQTPGH EVTRMLVSAH LSLYGMELNA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgacca ggaagacact cgccattgaa ggcctgaccg cgaccaccgt aatagacggg cagcagcgcg tccttgtcga taatctttct cttggcgttc aacgcggacg cattctcgca ctggtcggag caagcggttc cggcaagtcg atgacctgtt cggcagcact tggtgtcctg ccgcccggcg tcacggcctc gcgcgggcgt gtgacgatag atggggttcc ttatgcggca aacgcgctgc gcgggcggca tgtcgcgacc atcatgcaaa acccgcgcgg cgcctttaat cctgttcgca ccatgcgcga ccatgccatc gagacccttc aggcgcttgg caagctcagt tcaaatccgc aggatcagat agtgcattgt atgcgggcgg ccgggctgga agacgttaaa accatcctct ccttacatcc gttcgagatg agcggcggca tgttgcagcg gatgatgatt gcgctggcgc tgctcagtga ggcgccattc ctgtttgccg atgagccgac caccgatctt gatctcgtcg tccagcttcg ggttctggag cttctggaaa aactggtcga ggaacgcgac ctcggcattc ttcttgtaac ccatgatatg ggcgtcgtcg ctagactggc ccatgatgtc gccgtcctcg atcatggccg ccttatcgaa caggccccgg tgatggatat ctttcaaacg cccggacatg aggtcacacg catgctggtc agcgcgcatc tgtcgctcta cggtatggag ctcaatgcat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat