SSGCID: Target Page

BrsuA.10014.t

Nickel transport ATP-binding protein NikE (TC 3.A.1.5.3)

CENTER ID:	BrsuA.10014.t
ORGANISM:	Brucella suis 1330
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuA.10014.t.B1.GE36795	Full length( BrsuA.10014.t )		1	266

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruSui107850_3016
BV-BRC:	fig\|204722.5.peg.3016
RefSeq:	NP_699973.1
RefSeq:	YP_005614480.1
UniProt:	Q8FVN0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSLISADNIV KIYQSHSLVG ASARKTVLHD ISISIGQGET VALLGRSGCG KSTLARLLVG LERPTSGEVR FRGVPLTKLD RSGMKAFRRE VQLIFQDSPG AVNARSSVRA IIGEPLRHLT SLDETRREER IQELLRLVEL PPEIADRLPA QVSGGQLQRI CIARALAVNP KLIILDEAVS NLDIHLQASA LALLTKLQQE GGIAYLFVTH DLRLVQKFAA RCLVMDEGQI VEEIKTADLD SMRHPASRLL REAVLPPLPV RAVETN

NT Sequence

ATGAGCCTGA TCAGCGCAGA CAATATCGTC AAAATCTATC AATCACATTC GCTCGTGGGT GCGAGCGCGC GTAAGACTGT GCTGCATGAT ATATCGATTT CTATCGGGCA AGGGGAGACG GTTGCCCTTC TTGGCCGAAG TGGTTGCGGC AAGAGCACGC TTGCCCGGTT GCTGGTGGGG CTGGAACGGC CAACTTCCGG CGAGGTCCGG TTCAGGGGCG TGCCCCTGAC AAAACTCGAC CGTTCAGGCA TGAAGGCGTT CCGGCGCGAG GTCCAGCTCA TTTTTCAGGA TTCGCCCGGC GCCGTGAATG CCCGCTCCAG CGTTCGGGCG ATCATTGGGG AGCCTCTGCG GCATCTTACT TCTCTGGATG AAACCCGCAG AGAAGAGCGT ATTCAGGAGC TTTTGAGGCT GGTTGAGTTG CCACCGGAAA TCGCTGACCG CCTGCCTGCC CAGGTCAGTG GCGGGCAGTT GCAGCGGATT TGTATTGCCC GTGCCCTGGC TGTTAATCCA AAGCTGATTA TCCTCGATGA AGCGGTTTCC AATCTCGACA TTCATCTTCA GGCATCCGCG CTGGCGCTTC TGACCAAACT GCAACAGGAA GGCGGCATAG CCTATCTCTT CGTCACGCAT GATTTGAGGC TGGTGCAAAA GTTTGCCGCA AGGTGCCTCG TTATGGACGA AGGGCAAATC GTGGAAGAGA TCAAAACGGC CGACCTCGAC AGTATGAGGC ACCCGGCATC GCGCCTTTTA CGCGAGGCCG TGCTGCCGCC GCTGCCTGTT CGGGCTGTAG AGACCAAT

Details for BrsuA.10014.t.B1.GE36795

HARVESTED ON:	3/27/2013
SEQUENCED ON:	3/30/2013
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS LISADNIVKI YQSHSLVGAS ARKTVLHDIS ISIGQGETVA LLGRSGCGKS TLARLLVGLE RPTSGEVRFR GVPLTKLDRS GMKAFRREVQ LIFQDSPGAV NARSSVRAII GEPLRHLTSL DETRREERIQ ELLRLVELPP EIADRLPAQV SGGQLQRICI ARALAVNPKL IILDEAVSNL DIHLQASALA LLTKLQQEGG IAYLFVTHDL RLVQKFAARC LVMDEGQIVE EIKTADLDSM RHPASRLLRE AVLPPLPVRA VEXN

Validated NT Sequence

ttttnnnnnn nttaagaagg aganntacca tggctcacca ccaccaccac catatgagcc tgatcagcgc agacaatatc gtcaaaatct atcaatcaca ttcgctcgtg ggtgcgagcg cgcgtaagac tgtgctgcat gatatatcga tttctatcgg gcaaggggag acggttgccc ttcttggccg aagtggttgc ggcaagagca cgcttgcccg gttgctggtg gggctggaac ggccaacttc cggcgaggtc cggttcaggg gcgtgcccct gacaaaactc gaccgttcag gcatgaaggc gttccggcgc gaggtccagc tcatttttca ggattcgccc ggcgccgtga atgcccgctc cagcgttcgg gcgatcattg gggagcctct gcggcatctt acttctctgg atgaaacccg cagagaagag cgtattcagg agcttttgag gctggttgag ttgccaccgg aaatcgctga ccgcctgcct gcccaggtca gtggcgggca gttgcagcgg atttgtattg cccgtgccct ggctgttaat ccaaagctga ttatcctcga tgaagcggtt tccaatctcg acattcatct tcaggcatcc gcgctggcgc ttctgaccaa actgcaacag gaaggcggca tagcctatct cttcgtcacg catgatttga ggctggtgca aaagtttgcc gcaaggtgcc tcgttatgga cgaagggcaa atcgtggaag agatcaaaac ggccgacctc gacagtatga ggcacccggc atcgcgcctt ttacgcgagg ccgtgctgcc gccgctgcct gttcgggctg tagagancaa ttaagtgagt aananaggan ccnnnnnnnn nnnnannnnn nnnnaaaaaa annnnnnnnn nnnnnnnnnn naannnnnnn ncnnngnnnn nnnnnnngcg ngggnnngnn nnnnnnnnnn tnnactnnnn ttnnnnnnnn nncnnnnnnn nnnnnnntnn nnnnntnncc tcntcctnnn cnncnnnnnc cnnnnnnann nnnnnnnnnn ncnnnnncnn nnnnnnttnc ntncnnctnn nnnnncnncn nnnannnnnc tnnnncccnc nncnnnnnnn tannncnnnn nnnccccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc c

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcc tgatcagcgc agacaatatc gtcaaaatct atcaatcaca ttcgctcgtg ggtgcgagcg cgcgtaagac tgtgctgcat gatatatcga tttctatcgg gcaaggggag acggttgccc ttcttggccg aagtggttgc ggcaagagca cgcttgcccg gttgctggtg gggctggaac ggccaacttc cggcgaggtc cggttcaggg gcgtgcccct gacaaaactc gaccgttcag gcatgaaggc gttccggcgc gaggtccagc tcatttttca ggattcgccc ggcgccgtga atgcccgctc cagcgttcgg gcgatcattg gggagcctct gcggcatctt acttctctgg atgaaacccg cagagaagag cgtattcagg agcttttgag gctggttgag ttgccaccgg aaatcgctga ccgcctgcct gcccaggtca gtggcgggca gttgcagcgg atttgtattg cccgtgccct ggctgttaat ccaaagctga ttatcctcga tgaagcggtt tccaatctcg acattcatct tcaggcatcc gcgctggcgc ttctgaccaa actgcaacag gaaggcggca tagcctatct cttcgtcacg catgatttga ggctggtgca aaagtttgcc gcaaggtgcc tcgttatgga cgaagggcaa atcgtggaag agatcaaaac ggccgacctc gacagtatga ggcacccggc atcgcgcctt ttacgcgagg ccgtgctgcc gccgctgcct gttcgggctg tagagaccaa ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat